The Kitasato archives of experimental medicine最新文献_第2页

Industrial hygienic study on nursing activities investigation on heart rate and energy expenditure of cranial nerves and ICU ward nurses. 护理活动的工业卫生研究颅神经及ICU病房护士心率、能量消耗调查。

The Kitasato archives of experimental medicine Pub Date : 1993-04-01

S Irimagawa, S Imamiya

{"title":"Industrial hygienic study on nursing activities investigation on heart rate and energy expenditure of cranial nerves and ICU ward nurses.","authors":"S Irimagawa, S Imamiya","doi":"","DOIUrl":"","url":null,"abstract":"Using a Holter electrocardiograph (ECG), we calculated the heart rates of ward nurses during day shifts, a time in which various activities are undertaken. Energy expenditures were calculated from the heart rate data and nursing workloads were evaluated. The following results were obtained. 1) The energy expenditures per day were 3302 kcal in the cranial neurology ward, 3064 kcal in the ICU. 2) The energy expenditures during working hours were 1976 kcal for the day shift (510 minutes) in the cranial neurology ward, 1744 kcal in the ICU, 1867 kcal for the evening shift (495 min) in the cranial neurology ward, 1645 kcal for the evening shift in the ICU and 1947 kcal for the night shift (570 min) in the cranial neurology ward, 1839 kcal for the night shift in the ICU. 3) The highest energy expenditures for different activities were 949 kcal (78.0%) for day shift in direct nursing care in the cranial neurology ward, 722 kcal (43.8%) for the evening shift in assisting with medical examination and treatment in the ICU, 754 kcal (41.0%) for the night shift in assisting with medical examination and treatment in the ICU ward. 4) The longest working hours were 217 min (48.0%) for the day shift in direct nursing care in the cranial neurology ward, 252 min (50.9%) for the evening shift, 234 min (41.0%) for the night shift in assisting with medical examination and treatment in the ICU. The results indicate that the energy expenditures of ward nurses are higher than those of other workers. The reasons for this difference are discussed in this report.","PeriodicalId":76691,"journal":{"name":"The Kitasato archives of experimental medicine","volume":"65 Suppl ","pages":"91-8"},"PeriodicalIF":0.0,"publicationDate":"1993-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18966854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic polymorphism of HLA-DR in the Japanese population. 日本人群HLA-DR基因多态性研究。

The Kitasato archives of experimental medicine Pub Date : 1993-04-01

F Obata

{"title":"Genetic polymorphism of HLA-DR in the Japanese population.","authors":"F Obata","doi":"","DOIUrl":"","url":null,"abstract":"As a result of carrying out sequence analyses on the HLA-DR genes of several Japanese donors, we found three new DRB1 alleles, DRB1-12b, DRB1-14c, and DRB1-JX6, that had not been identified using immunological procedures. Sequence-specific oligonucleotide (SSO) probes directed against various DRB1 alleles, including the above three, enabled DNA typing of all the DRB types in the Japanese population and calculation of their gene frequencies based on this typing to be carried out for the first time. The SSO-DNA typing yielded higher DR13 and DR14 gene frequencies than those reported by serological workshops. Next, we applied this DR-DNA typing to the analysis of Japanese patients with juvenile rheumatoid arthritis (JRA) and found that DRB1*0405 was the allele susceptible to rheumatoid factor-positive polyarticular JRA, which is one of the four types of JRA that has been classified clinically. Analysis of the DR types of patients who suffered unexplained recurrent spontaneous abortion (URSA) using DNA typing demonstrated that 1) URSA is not correlated with any particular DR type and 2) no difference between the DR sharing rate of patients and normal couple was detected, which contradicts the results obtained in some serological studies.","PeriodicalId":76691,"journal":{"name":"The Kitasato archives of experimental medicine","volume":"65 Suppl ","pages":"13-24"},"PeriodicalIF":0.0,"publicationDate":"1993-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18961855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of sodium and chloride ions on blood pressure in deoxycorticosterone acetate-treated rats. 钠和氯离子对醋酸脱氧皮质酮处理大鼠血压的影响。

The Kitasato archives of experimental medicine Pub Date : 1993-04-01

A Kadota, Y Aoki, N Ishii, K Numakami, Z Ogawa, H Itoh, K Mitsuta, M Kohno, H Ikenaga, T Saruta

{"title":"Effects of sodium and chloride ions on blood pressure in deoxycorticosterone acetate-treated rats.","authors":"A Kadota, Y Aoki, N Ishii, K Numakami, Z Ogawa, H Itoh, K Mitsuta, M Kohno, H Ikenaga, T Saruta","doi":"","DOIUrl":"","url":null,"abstract":"The effects of sodium (Na+) and chloride ions (Cl-) on blood pressure were studied in rats treated with deoxycorticosterone acetate (DOCA). Four groups were prepared, each consisting of male Wistar rats that underwent heminephrectomy and administration of DOCA: the control group was maintained with tap water, the NaCl group with tap water containing 1% sodium chloride, the NaCit group with tap water containing 1.67% sodium citrate (including an equivalent dose of Na+ to 1% NaCl), and the ChoCl group with tap water containing 1.15% choline chloride (including an equivalent dose of Cl- to 1% NaCl). The time-course of systolic blood pressure showed only slight change in blood pressure in the control and ChoCl groups, and in the NaCl and NaCit groups. The rotational correlation time, an index of the fluidity of erythrocyte membrane, with spin-labeling of 16-doxyl-stearic acid, was significantly (p < 0.05) higher in the NaCl and NaCit groups than in the control group, indicating an increase in the membrane fluidity, i.e., membrane fragility. The sodium, potassium ions-activated adenosine triphosphatase (Na+,K(+)-ATPase) activity of the erythrocyte membrane was decreased to 22% (P < 0.01) and 24% (P < 0.01) in the NaCl and NaCit groups, respectively, compared with the control groups; this activity was decreased to 43% in the ChoCl group (P < 0.05). The Ca(2+)-ATPase activity showed similar changes. In contrast, there were no marked differences in the erythrocyte electrolyte level between the groups.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":76691,"journal":{"name":"The Kitasato archives of experimental medicine","volume":"65 Suppl ","pages":"65-72"},"PeriodicalIF":0.0,"publicationDate":"1993-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18966851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of Babesia gibsoni in the salivary glands of the tick, Haemaphysalis longicornis. 长角血蜱唾液腺中吉氏巴贝斯虫的发展。

The Kitasato archives of experimental medicine Pub Date : 1993-04-01

S Higuchi, H Hoshina, F Hoshi, S Kawamura, Y Yasuda

引用次数: 0

Industrial hygienic study on nursing activities comparison of energy expenditure between pedometer and Holter electrocardiograph. 工业卫生护理活动的研究计步器与动态心电图能量消耗的比较。

The Kitasato archives of experimental medicine Pub Date : 1993-04-01

S Irimagawa, S Imamiya

{"title":"Industrial hygienic study on nursing activities comparison of energy expenditure between pedometer and Holter electrocardiograph.","authors":"S Irimagawa, S Imamiya","doi":"","DOIUrl":"","url":null,"abstract":"Few objective and quantitative studies have been made on energy expenditure during hospital nursing activities. The pedometer is a light handy instrument, which gives highly accurate results, and is a minimal burden for subjects. To ascertain daily energy expenditures, we used a pedometer. Twelve day-shift nurses served as subjects, in whom the number of steps token in 16 hours, from getting up until going to bed, was measured. We used the data obtained to calculate energy expenditures for 24 hours. In addition, eight nurses were chosen from among these subjects to undergo simultaneous pedometry and Holter electrocardiography. These data were also used to calculate energy expenditures. The following results were obtained. 1) The maximum total number of steps was 13897, the minimum 9899 and the mean +/- standard deviation was 11478.4 +/- 1285.8. 2) As to the energy expenditure of the eight nurses who were both pedometer and Holter electrocardiograph, the highest energy expenditure calculated from pedometry results was 2648 kcal. the lowest 2455 kcal, and the mean +/- standard deviation was 2514.1 +/- 55.5. The highest energy calculated from Holter electrocardiographic data was 3040 kcal, the lowest 2683 kcal, and the mean +/- standard deviation was 2868.1 +/- 108.0. The correlation coefficient was r = 0.59, (P < 0.05).","PeriodicalId":76691,"journal":{"name":"The Kitasato archives of experimental medicine","volume":"65 Suppl ","pages":"99-105"},"PeriodicalIF":0.0,"publicationDate":"1993-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18966855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Methods for detection of MDR1 mRNA expression on acute myelogenous leukemia cells. 急性髓性白血病细胞MDR1 mRNA表达的检测方法。

The Kitasato archives of experimental medicine Pub Date : 1993-04-01

H Sato, T Oonishi, C Wada

{"title":"Methods for detection of MDR1 mRNA expression on acute myelogenous leukemia cells.","authors":"H Sato, T Oonishi, C Wada","doi":"","DOIUrl":"","url":null,"abstract":"Overexpression of the human multidrug resistance gene (MDR1) on acute myelogenous leukemia (AML) correlates with poor prognosis. We evaluated several methods for mRNA estimation to standardize simple and reliable techniques for identifying MDR1 positive leukemia among untreated AMLs in large scale studies. Northern blot detection of MDR1 mRNA suffered from low signal-to-noise ratio under the conventional conditions, that was improved mainly by removing unincorporated radioactivity. The amount of MDR1 transcripts on positive cells was estimated less than 10% of that of constitutive mRNA species. A modified method seemed useful in estimating the total amount of the MDR1 mRNA in a whole leukemic cell population, and suitable to study stock samples or for large prospective clinical trials. RT-PCR was more sensitive in detecting MDR1 mRNA than Northern blot analysis, and the very feature made it virtually impossible to exclude contamination with normal hematopoietic cells. This procedure showed that FAB M3 leukemias were essentially MDR1 negative, and there existed frequently myelodysplastic syndrome subpopulation which had excessive MDR1 transcripts. In situ hybridization of the mRNA with a FITC-labeled phosphorothioate oligonucleotide probe was visualized using flowcytometry or con-focus lightmicroscopy, enabled us to recognize the difference between multidrug resistant K562/ADM and its wild type.","PeriodicalId":76691,"journal":{"name":"The Kitasato archives of experimental medicine","volume":"65 Suppl ","pages":"25-36"},"PeriodicalIF":0.0,"publicationDate":"1993-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18961859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of beta-blocking agents on the release of various enzymes in muscular tissues. 阻滞剂对肌肉组织中各种酶释放的影响。

The Kitasato archives of experimental medicine Pub Date : 1993-04-01

H Itoh, Y Jingu, K Numakami, T Saruta

引用次数: 0

Chemical and pharmacological studies on efficacy of Japanese and Chinese herbal medicines. 日本和中国中草药药效的化学和药理研究。

The Kitasato archives of experimental medicine Pub Date : 1992-12-01

H Yamada

引用次数: 0

Effect of short-term fasting treatment on liver and renal function. 短期禁食治疗对肝肾功能的影响。

The Kitasato archives of experimental medicine Pub Date : 1992-12-01

T Horiuchi, M Tsuchida, Y Kondo, T Sasaki