{"title":"[The Herbst-Volkheimer effect].","authors":"O Prokop","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>More than 150 years ago the foundations were laid for the so-called HERBST effect which was subsequently forgotten. In the sixties the phenomenon was rediscovered by VOLKHEIMER at the Charité Hospital in Berlin and then reviewed through many experiments and publications. What is meant by the HERBST effect? If an experimental animal or even human being is given a larger amount of maize starch or also biscuits or some other products containing starch, starch bodies can be detected rapidly in venous blood already after minutes or half an hour later and in the urine after one hour and later. The term \"persorption\" has been coined for this interesting phenomenon. It is indeed surprising that it has met with so little attention. As a matter of fact, it constitutes the basis for our understanding of peroral immunization and of allergies. In the same way, feeding of carbon particles results in their appearance and detection in blood, kidney and urine. The same result is obtained by the intake of diatoms and what is even more important with meat fibres. I hope you are aware of the implications. When Professor NAGAI stayed in Berlin, we tried to receive the phenomenon. Since only a few cell nuclei are necessary for \"genetic fingerprinting\" we thought that after intake of 200 or 400 g of raw meat the type of food eaten could be determined from the urinary sediment by means of the fingerprint method which would be of forensic significance. Therefore, we eat meat and raw liver and examined the urinary sediment.(ABSTRACT TRUNCATED AT 250 WORDS)</p>","PeriodicalId":76691,"journal":{"name":"The Kitasato archives of experimental medicine","volume":"63 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"1990-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13424546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M Kondoh, Y Aoki, Z Ogawa, Y Hata, H Suzuki, H Itoh
{"title":"Effect of chlorpromazine on transmigration of mitochondrial aspartate aminotransferase in rat liver.","authors":"M Kondoh, Y Aoki, Z Ogawa, Y Hata, H Suzuki, H Itoh","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Cholestatic liver injury was experimentally induced in rats by administration of chlorpromazine (CPZ). The peak activity of mitochondrial aspartate aminotransferase (AST) released in serum was found to precede the peak of total AST activity. The liver mitochondria obtained from rats treated with CPZ was fractionated into two subfractions: one containing the intermembrane space, and the other containing the matrix and the membranes. As a results, the relative activity of AST in the intermembrane space was significantly increased at 12 h after CPZ administration. This result suggests that mitochondrial AST, which is dominantly located in the mitochondrial matrix, transmigrated to the intermembrane space via the inner membrane under the effect of CPZ administration.</p>","PeriodicalId":76691,"journal":{"name":"The Kitasato archives of experimental medicine","volume":"62 4","pages":"181-6"},"PeriodicalIF":0.0,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13783235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K Takehara, C Kawai, A Seki, N Hashimoto, M Yoshimura
{"title":"Identification and characterization of an avian reovirus isolated from black-tailed gull (Larus crassirostris).","authors":"K Takehara, C Kawai, A Seki, N Hashimoto, M Yoshimura","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>An isolated virus from the feces of nestlings of Black-tailed gulls living on the Kabu-Island, Hachinohe-city, Aomori-prefecture, was identified as an avian reovirus from its morphological, physicochemical and biological features. Serologically, the isolate has a 62% of relatedness to TS-17 strain, a prototype of avian reovirus in Japan. The isolate showed no significant virulences to one-day-old SPF chickens, and showed low mortalities to chicken embryos, although it formed remarkable lesion onto chorioallantoic membrane.</p>","PeriodicalId":76691,"journal":{"name":"The Kitasato archives of experimental medicine","volume":"62 4","pages":"187-98"},"PeriodicalIF":0.0,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13705447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Pseudomonas fluorescens isolated from the diseased rainbow trout, Oncorhynchus mykiss.","authors":"M Sakai, S Atsuta, M Kobayashi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Pseudomonas fluorescens was isolated from the diseased rainbow trout, Oncorhynchus mykiss, in Iwate Prefecture. Typical symptoms of the diseased fish were hemorrhage at the bases of fin and anal region, and internally, petechia of each organ, especially the intestine. This bacterium was pathogenic to rainbow trout and Tilapia, Sarotherodon niloticus.</p>","PeriodicalId":76691,"journal":{"name":"The Kitasato archives of experimental medicine","volume":"62 4","pages":"157-62"},"PeriodicalIF":0.0,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13664509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M Doi, H Kuramoto, S Hasegawa, Y Tanaka, Y Fujisaki
{"title":"A modified method for the determination of aluminium-gel in vaccine.","authors":"M Doi, H Kuramoto, S Hasegawa, Y Tanaka, Y Fujisaki","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The conventional method of aluminium analysis, described in the Biological Preparation Standard for Animals, was found to give erroneous results in the case of vaccine containing aluminium-gel as an adjuvant. This is because of incomplete decomposition of the gel and unsettled reaction system for the determination. But these problems were settled definitely. Heating on a direct fire for 15 min, by which temperature of the content attained 115-120 degrees C at atmospheric pressure, allowed the gel to complete decomposition and the result came to close the theoretical value. And a constant result by the decomposition itself was obtained with 2 volumes of nitric acid against 1 volume indicated in the standard method. Quantitative reaction of the gel-decomposed solution did hold in range of about 0.75-2.0 micrograms aluminium per ml. Use of acetate buffer solution for dilution of the decomposed solution following by sampling in 2 volumes of the dilution into the reaction mixture made the variation to the least as compared with the water-dilution following by 1 volume sampling indicated in the standard method. By these modified conditions, we succeeded in reducing the relative error and the coefficient of variation to less than +/- 5% and +/- 2% respectively.</p>","PeriodicalId":76691,"journal":{"name":"The Kitasato archives of experimental medicine","volume":"62 4","pages":"171-80"},"PeriodicalIF":0.0,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13783234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Serological comparison of Kagoshima and Chuzan viruses of Palyam serogroup orbivirus isolated in Japan.","authors":"H Kurogi, M Matumoto","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76691,"journal":{"name":"The Kitasato archives of experimental medicine","volume":"62 4","pages":"199-201"},"PeriodicalIF":0.0,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13783236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The influence of Hymenolepis nana infection on antibody responses to sheep red blood cells in mice.","authors":"M Shinoda, K Asano","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The influence of Hymenolepis nana infection on the primary antibody responses to both sheep red blood cells (SRBC) and dinitrophenylated Ficoll-coated SRBC (DNP-coated SRBC) was examined in BALB/c mice. The in vitro cultures of spleen cells (Sp C) obtained from mice inoculated with either 100 or 1,000 eggs developed fewer plaque forming cells (PFC) than those of Sp C from non-infected mice, when the cells were exposed to SRBC. Thereafter, the number of PFC was gradually increased and almost reached the initial levels by day 21. On the other hand, the ability of Sp C obtained from mice infected with 10,000 eggs to produce PFC gradually dropped as the course of infection proceeded. However, H. nana infection did not reduce the capacity of Sp C to produce PFC to DNP-coated SRBC. Addition of T-cell enriched population from infected mice, which was obtained by passing through a nylon wool column, to anti-Thy-1.2 antibody treated non-infected Sp C significantly inhibited the production of PFC to SRBC. These results strongly suggest that H. nana infection impairs T-cell function in mice.</p>","PeriodicalId":76691,"journal":{"name":"The Kitasato archives of experimental medicine","volume":"62 4","pages":"163-9"},"PeriodicalIF":0.0,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13678607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Survival of microfilariae released in vitro by the filarial worm, Dipetalonema viteae.","authors":"J Maki, P P Weinstein","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76691,"journal":{"name":"The Kitasato archives of experimental medicine","volume":"62 4","pages":"203-5"},"PeriodicalIF":0.0,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13783237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}