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Induction of sensory neurons from neuroepithelial stem cells by the ISX9 small molecule. ISX9小分子诱导神经上皮干细胞产生感觉神经元。
IF 1.8
American journal of stem cells Pub Date : 2016-05-15 eCollection Date: 2016-01-01
Rouknuddin Qasim Ali, Evelina Blomberg, Anna Falk, Lars Ährlund-Richter, Mats Ulfendahl
{"title":"Induction of sensory neurons from neuroepithelial stem cells by the ISX9 small molecule.","authors":"Rouknuddin Qasim Ali,&nbsp;Evelina Blomberg,&nbsp;Anna Falk,&nbsp;Lars Ährlund-Richter,&nbsp;Mats Ulfendahl","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Hearing impairment most often involves loss of sensory hair cells and auditory neurons. As this loss is permanent in humans, a cell therapy approach has been suggested to replace damaged cells. It is thus of interest to generate lineage restricted progenitor cells appropriate for cell based therapies. Human long-term self-renewing neuroepithelial stem (lt-NES) cell lines exhibit in vitro a developmental potency to differentiate into CNS neural lineages, and importantly lack this potency in vivo, i.e do not form teratomas. Small-molecules-driven differentiation is today an established route obtain specific cell derivatives from stem cells. In this study, we have investigated the effects of three small molecules SB431542, ISX9 and Metformin to direct differentiation of lt-NES cells into sensory neurons. Exposure of lt-NES cells to Metformin or SB431542 did not induce any marked induction of markers for sensory neurons. However, a four days exposure to the ISX9 small molecule resulted in reduced expression of NeuroD1 mRNA as well as enhanced mRNA levels of GATA3, a marker and important player in auditory neuron specification and development. Subsequent culture in the presence of the neurotrophic factors BDNF and NT3 for another seven days yielded a further increase of mRNA expression for GATA3. This regimen resulted in a frequency of up to 25-30% of cells staining positive for Brn3a/Tuj1. We conclude that an approach with ISX9 small molecule induction of lt-NES cells into auditory like neurons may thus be an attractive route for obtaining safe cell replacement therapy of sensorineural hearing loss. </p>","PeriodicalId":7657,"journal":{"name":"American journal of stem cells","volume":"5 1","pages":"19-28"},"PeriodicalIF":1.8,"publicationDate":"2016-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4913294/pdf/ajsc0005-0019.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34604490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Modern Alchemy: Cellular Reprogramming and Transdifferentiation 现代炼金术:细胞重编程和转分化
IF 1.8
American journal of stem cells Pub Date : 2016-01-01 DOI: 10.28967/jscrt.2016.01.16001
Summer A Helmi, D. Rancourt
{"title":"Modern Alchemy: Cellular Reprogramming and Transdifferentiation","authors":"Summer A Helmi, D. Rancourt","doi":"10.28967/jscrt.2016.01.16001","DOIUrl":"https://doi.org/10.28967/jscrt.2016.01.16001","url":null,"abstract":"","PeriodicalId":7657,"journal":{"name":"American journal of stem cells","volume":"1 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69316575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Transdifferentiation of endothelial cells to smooth muscle cells play an important role in vascular remodelling. 内皮细胞向平滑肌细胞的转分化在血管重构中起着重要的作用。
IF 1.8
American journal of stem cells Pub Date : 2015-03-15 eCollection Date: 2015-01-01
Núria Coll-Bonfill, Melina Mara Musri, Victor Ivo, Joan Albert Barberà, Olga Tura-Ceide
{"title":"Transdifferentiation of endothelial cells to smooth muscle cells play an important role in vascular remodelling.","authors":"Núria Coll-Bonfill,&nbsp;Melina Mara Musri,&nbsp;Victor Ivo,&nbsp;Joan Albert Barberà,&nbsp;Olga Tura-Ceide","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Pulmonary artery remodelling it is a major feature of pulmonary hypertension (PH). It is characterised by cellular and structural changes of the pulmonary arteries causing higher pulmonar vascular resistance and right ventricular failure. Abnormal deposition of smooth muscle-like (SM-like) cells in normally non-muscular, small diameter vessels and a deregulated control of endothelial cells are considered pathological features of PH. The origin of the SM-like cells and the mechanisms underlying the development and progression of this remodelling process are not understood. Endothelial cells within the intima may migrate from their organised layer of cells and transition to mesenchymal or SM-like phenotype in a process called endothelial-mesenchymal transition (EnMT). Traditionally, Waddington's epigenetic landscape illustrates that fates of somatic cells are progressively determined to compulsorily follow a downhill differentiation pathway. EnMT induces the transformation of cells with stem cell traits, therefore contrasting Waddington's theory and confirming that cell fate seems to be far more flexible than previously thought. The prospect of therapeutic inhibition of EnMT to delay or prevent PH may represent a promising new treatment modality. </p>","PeriodicalId":7657,"journal":{"name":"American journal of stem cells","volume":"4 1","pages":"13-21"},"PeriodicalIF":1.8,"publicationDate":"2015-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4396157/pdf/ajsc0004-0013.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33182756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The effect of autologous bone marrow mononuclear cell transplantation on the survival duration in Amyotrophic Lateral Sclerosis - a retrospective controlled study. 自体骨髓单个核细胞移植对肌萎缩性侧索硬化症患者存活时间的影响——一项回顾性对照研究。
IF 1.8
American journal of stem cells Pub Date : 2015-03-15 eCollection Date: 2015-01-01
Alok K Sharma, Hemangi M Sane, Amruta A Paranjape, Nandini Gokulchandran, Anjana Nagrajan, Myola D'sa, Prerna B Badhe
{"title":"The effect of autologous bone marrow mononuclear cell transplantation on the survival duration in Amyotrophic Lateral Sclerosis - a retrospective controlled study.","authors":"Alok K Sharma,&nbsp;Hemangi M Sane,&nbsp;Amruta A Paranjape,&nbsp;Nandini Gokulchandran,&nbsp;Anjana Nagrajan,&nbsp;Myola D'sa,&nbsp;Prerna B Badhe","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Amyotrophic Lateral Sclerosis (ALS) is a rapidly progressive neurodegenerative disorder with fatal prognosis. Cellular therapy has been studied for ALS in various animal models and these advances have highlighted its potential to be a treatment modality. This is a retrospective controlled cohort study of total 57 patients. Out of these, 37 patients underwent autologous bone marrow mononuclear cell transplantation in addition to standard rehabilitation and Riluzole. Control group consisted of 20 patients who did not receive cell transplantation. The survival duration since the onset of the disease for both the groups was computed using a Kaplan-Meier Survival analysis and compared using log-rank test. Effect of age at onset, type of onset and lithium on survival duration in the intervention group was analyzed. Mean survival duration of patients in intervention group was 87.76 months which was higher than the control group mean survival duration of 57.38 months. Survival duration was significantly (p = 0.039) higher in people with the onset of the disease below 50 years of age. Limb onset and lithium also showed positive influence on the survival duration. Mean survival duration of the intervention group was also higher than the survival duration of ALS patients in previous epidemiological studies. In addition to the standard treatment with Riluzole, early intervention with combination of BMMNCs transplantation and Lithium may have a positive effect on the survival duration in ALS. Prospective randomized controlled studies with a larger sample size and rigorous methodology are required for conclusive findings. </p>","PeriodicalId":7657,"journal":{"name":"American journal of stem cells","volume":"4 1","pages":"50-65"},"PeriodicalIF":1.8,"publicationDate":"2015-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4396155/pdf/ajsc0004-0050.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33182762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Adipose tissue derived mesenchymal stem cells for musculoskeletal repair in veterinary medicine. 脂肪组织源性间充质干细胞在兽医学肌肉骨骼修复中的应用。
IF 1.8
American journal of stem cells Pub Date : 2015-03-15 eCollection Date: 2015-01-01
Stefan Arnhold, Sabine Wenisch
{"title":"Adipose tissue derived mesenchymal stem cells for musculoskeletal repair in veterinary medicine.","authors":"Stefan Arnhold,&nbsp;Sabine Wenisch","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Adipose tissue derived stem cells (ASCs) are mesenchymal stem cells which can be obtained from different adipose tissue sources within the body. It is an abundant cell pool, which is easy accessible and the cells can be obtained in large numbers, cultivated and expanded in vitro and prepared for tissue engineering approaches, especially for skeletal tissue repair. In the recent years this cell population has attracted a great amount of attention among researchers in human as well as in veterinary medicine. In the meantime ASCs have been well characterized and their use in regenerative medicine is very well established. This review focuses on the characterization of ASCs for their use for tissue engineering approaches especially in veterinary medicine and also highlights a selection of clinical trials on the basis of ASCs as the relevant cell source. </p>","PeriodicalId":7657,"journal":{"name":"American journal of stem cells","volume":"4 1","pages":"1-12"},"PeriodicalIF":1.8,"publicationDate":"2015-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4396154/pdf/ajsc0004-0001.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33182755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Stem cells for the cell and molecular therapy of type 1 diabetes mellitus (T1D): the gap between dream and reality. 干细胞用于1型糖尿病(T1D)的细胞和分子治疗:梦想与现实的差距。
IF 1.8
American journal of stem cells Pub Date : 2015-03-15 eCollection Date: 2015-01-01
Riccardo Calafiore, Giuseppe Basta
{"title":"Stem cells for the cell and molecular therapy of type 1 diabetes mellitus (T1D): the gap between dream and reality.","authors":"Riccardo Calafiore,&nbsp;Giuseppe Basta","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In spite of intense research, over the past 2-3 decades, targeted to validating methods for the cure of T1D, based on cell substitution therapy in the place of exogenously administered insulin injections, achievement of the final goal continues to remain out of reach. In fact, aside of very limited clinical success of the few clinical trials of pancreatic islet cell transplantation in totally immunosuppressed patients with T1D, the vast majority of these diabetic patients invariably is insulin-dependent. New advances for cell and molecular therapy for T1D, including use of stem cells, are reviewed and discussed in an attempt to clearly establish where we are and where are we may go for the final cure for T1DM. </p>","PeriodicalId":7657,"journal":{"name":"American journal of stem cells","volume":"4 1","pages":"22-31"},"PeriodicalIF":1.8,"publicationDate":"2015-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4396156/pdf/ajsc0004-0022.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33182759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Experimental approaches to derive CD34+ progenitors from human and nonhuman primate embryonic stem cells. 从人类和非人类灵长类胚胎干细胞中获得CD34+祖细胞的实验方法。
IF 1.8
American journal of stem cells Pub Date : 2015-03-15 eCollection Date: 2015-01-01
Qiang Shi, John L VandeBerg
{"title":"Experimental approaches to derive CD34+ progenitors from human and nonhuman primate embryonic stem cells.","authors":"Qiang Shi,&nbsp;John L VandeBerg","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Traditionally, CD34 positive cells are predominantly found in the umbilical cord and bone marrow, thus are considered as hematopoietic progenitors. Increasing evidence has suggested that the CD34+ cells represent a distinct subset of cells with enhanced progenitor activity; CD34 is a general marker of progenitor cells in a variety of cell types. Because the CD34 protein shows expression early on in hematopoietic and vascular-associated tissues, CD34+ cells have enormous potential as cellular agents for research and for clinical cell transplantation. Directed differentiation of embryonic stem cells will give rise to an inexhaustible supply of CD34+ cells, creating an exciting approach for biomedical research and for regenerative medicine. Here, we review the main methods that have been published for the derivation of CD34+ cells from embryonic stem cells; specifically those approaches the human and nonhuman primate stem cells. We summarize current status of this field, compare the methods used, and evaluate the issues in translating the bench science to bedside therapy. </p>","PeriodicalId":7657,"journal":{"name":"American journal of stem cells","volume":"4 1","pages":"32-7"},"PeriodicalIF":1.8,"publicationDate":"2015-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4396158/pdf/ajsc0004-0032.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33182760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An effective freezing/thawing method for human pluripotent stem cells cultured in chemically-defined and feeder-free conditions. 一种在化学定义和无饲料条件下培养的人多能干细胞的有效冷冻/解冻方法。
IF 1.8
American journal of stem cells Pub Date : 2015-03-15 eCollection Date: 2015-01-01
Naoki Nishishita, Marie Muramatsu, Shin Kawamata
{"title":"An effective freezing/thawing method for human pluripotent stem cells cultured in chemically-defined and feeder-free conditions.","authors":"Naoki Nishishita,&nbsp;Marie Muramatsu,&nbsp;Shin Kawamata","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Culturing human Pluripotent Stem Cells (hPSC)s in chemically defined medium and feeder-free condition can facilitate metabolome and proteome analysis of culturing cells and medium, and reduce regulatory concerns for clinical application of cells. And in addition, if hPSC are passaged and cryopreserved in single cells it also facilitates quality control of cells at single cell level. Here we report a robust single cell freezing and thawing method of hPSCs cultured in chemically-defined medium TeSR(TM)-E8(TM) and on cost-effective recombinant human Vitronectin-N (rhVTN-N)-coated dish. Cells are dissociated into single cells with recombinant TrypLE(TM) Select and 0.5 mM EDTA/PBS (3:1 solution) in the presence of Rock inhibitor and cryopreserved with chemically defined CryoStem(TM). Approximately 60% of cells were viable after dissociation. Aggrewell(TM) 400 was used to form cell clumps of 500 cells after thaw in the presence of Rock inhibitor and cells were cultured for two days with TeSR-E8. Cells clumps were then seeded on rhVTN-N-coated dish and cultured with TeSR-E8 for two days prior to the first passage after thawing. Number of viable cells at the first passage increased around 10 times of that just before freezing. This robust single cell freezing method for hPSCs cultured in chemically defined medium will facilitate quality control of cultured cells at single cell level before cryopreservation and consequently assure the quality of cells in frozen vials for further manipulation after thawing. </p>","PeriodicalId":7657,"journal":{"name":"American journal of stem cells","volume":"4 1","pages":"38-49"},"PeriodicalIF":1.8,"publicationDate":"2015-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4396159/pdf/ajsc0004-0038.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33182761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Metastatic cancer stem cells: from the concept to therapeutics. 转移性癌症干细胞:从概念到治疗方法。
IF 1.8
American journal of stem cells Pub Date : 2014-09-05 eCollection Date: 2014-01-01
Wen-Ting Liao, Ya-Ping Ye, Yong-Jian Deng, Xiu-Wu Bian, Yan-Qing Ding
{"title":"Metastatic cancer stem cells: from the concept to therapeutics.","authors":"Wen-Ting Liao,&nbsp;Ya-Ping Ye,&nbsp;Yong-Jian Deng,&nbsp;Xiu-Wu Bian,&nbsp;Yan-Qing Ding","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Metastatic cancer stem cells (MCSCs) refer to a subpopulation of cancer cells with both stem cell properties and invasion capabilities that contribute to cancer metastasis. MCSCs have capability of self-renewal, potentials of multiple differentiation and development and/or reconstruction of cancer tissues. As compared with stationary cancer stem cells, MCSCs are capable of invasion to normal tissues such as vasculatures, resistance to chemo- and/or radio-therapies, escape from immune surveillance, survival in circulation and formation of metastasis. MCSCs are derived from invasive cancer stem cells (iCSCs) due to the plasticity of cancer stem cells, which is one of the characteristics of cancer cell heterogeneity. Both stages of iCSCs and MSCSs are the potential therapeutic targets for cancer metastasis in the future strategies of personalized cancer therapy. </p>","PeriodicalId":7657,"journal":{"name":"American journal of stem cells","volume":"3 2","pages":"46-62"},"PeriodicalIF":1.8,"publicationDate":"2014-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4163604/pdf/ajsc0003-0046.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32677517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Emerging paradigms of long non-coding RNAs in gastrointestinal cancer. 长链非编码rna在胃肠道肿瘤中的新范式。
IF 1.8
American journal of stem cells Pub Date : 2014-09-05 eCollection Date: 2014-01-01
Hui-Juan Jiang, Shuang Wang, Yanqing Ding
{"title":"Emerging paradigms of long non-coding RNAs in gastrointestinal cancer.","authors":"Hui-Juan Jiang,&nbsp;Shuang Wang,&nbsp;Yanqing Ding","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A large number of long non-coding RNAs (lncRNAs) have been discovered by genome-wide transcriptional analyses. Emerging evidence has indicated that lncRNAs regulate gene expression at epigenetic, transcription, and post-transcription levels, are widely involved in various pathobiology of human diseases, and may play an important role in the biology of cancer stem cells. Alterations of specific lncRNAs have been revealed to interact with the major pathways of cell proliferation, apoptosis, differentiation, invasion and metastasis in many human malignancies, such as gastrointestinal cancer. This review summarizes the current understandings in biological functions and implications of lncRNAs in gastrointestinal cancer. </p>","PeriodicalId":7657,"journal":{"name":"American journal of stem cells","volume":"3 2","pages":"63-73"},"PeriodicalIF":1.8,"publicationDate":"2014-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4163605/pdf/ajsc0003-0063.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32677516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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