Andrew Liu, Jessica O'Connell, Farley Wall, Richard W Carthew
{"title":"Scaling between cell cycle duration and wing growth is regulated by Fat-Dachsous signaling in <i>Drosophila</i>.","authors":"Andrew Liu, Jessica O'Connell, Farley Wall, Richard W Carthew","doi":"10.1101/2023.08.01.551465","DOIUrl":"10.1101/2023.08.01.551465","url":null,"abstract":"<p><p>The atypical cadherins Fat and Dachsous (Ds) signal through the Hippo pathway to regulate growth of numerous organs, including the <i>Drosophila</i> wing. Here, we find that Ds-Fat signaling tunes a unique feature of cell proliferation found to control the rate of wing growth during the third instar larval phase. The duration of the cell cycle increases in direct proportion to the size of the wing, leading to linear-like growth during the third instar. Ds-Fat signaling enhances the rate at which the cell cycle lengthens with wing size, thus diminishing the rate of wing growth. We show that this results in a complex but stereotyped relative scaling of wing growth with body growth in <i>Drosophila</i>. Finally, we examine the dynamics of Fat and Ds protein distribution in the wing, observing graded distributions that change during growth. However, the significance of these dynamics is unclear since perturbations in expression have negligible impact on wing growth.</p>","PeriodicalId":76429,"journal":{"name":"Revista brasileira de biologia","volume":"60 3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11030236/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89289383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Influence of the pineal gland on the physiology, morphometry and morphology of pancreatic islets in rats","authors":"L. M. B. Lima, L. Reis, M. A. D. Lima","doi":"10.1590/S0034-71082001000200018","DOIUrl":"https://doi.org/10.1590/S0034-71082001000200018","url":null,"abstract":"To investigate the influence of the pineal gland through melatonin secretion on the physiological and morphological parameters of pancreatic islets, we studied the plasma biochemistry and morphological and morphometric characteristics of the endocrine pancreas of male Wistar rats. The animals were distributed into five groups of ten rats each: NC - normal control group; NS - sham-operated group; Px (25) - pinealectomised group, studied 15-25 days after surgery; Px (70) - pinealectomised group, studied 60-70 days after surgery; ALX - alloxan monohydrate-treated group. Data are analyzed statistically by ANOVA and by the Kruskal-Wallis test. Although there was no significant difference in plasma glucose or insulin levels between the Px (25), Px (70) and NC groups, Px (25) animals showed a tendency to increased glucose and reduced insulin levels. The ALX group showed a clear elevation of plasma glucose and a reduction of plasma insulin compared to the other groups. Morphometric analysis showed a larger pancreatic islet area and a lower pancreatic islet density in the pancreas of Px (70) animals and an increase in degenerative pathological processes in the pancreatic islets of the Px (25) and ALX groups. The present results suggest that melatonin, in addition to acting on tissue sensitivity to insulin (as reported in other studies), affects the secretory action of beta cells, as demonstrated by the morphological and morphometric changes observed in pinealectomised animals.","PeriodicalId":76429,"journal":{"name":"Revista brasileira de biologia","volume":"61 1","pages":"333-340"},"PeriodicalIF":0.0,"publicationDate":"2001-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1590/S0034-71082001000200018","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67178073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Dissolved inorganic carbon and metabolism of an eutrophic lacustrine system: variations from a 36-hours study","authors":"P. Pedrosa, C. Rezende","doi":"10.1590/S0034-71082000000400010","DOIUrl":"https://doi.org/10.1590/S0034-71082000000400010","url":null,"abstract":"Este artigo fornece estimativas de produtividade primaria e mineralizacao liquidas ¾ metabolismo liquido do carbono ¾ ocorrentes na Lagoa de Cima (Estado do Rio de Janeiro), em funcao das variacoes das concentracoes de carbono inorgânico dissolvido (CID) no meio pelagico. As concentracoes de CID abrangeram o intervalo entre 203 e 773 µmol L-1 e o %CO2(aq) (saturacao) entre 339 e 4.901. O metabolismo liquido do carbono por periodo individual (claro/escuro) variou entre -556 e 503 mmol DIC m-2 12 h-1 no intervalo de 36 horas de estudo. Tambem foram observadas variacoes acentuadas no metabolismo liquido do carbono para ciclos de 24 horas. Os valores estenderam-se entre -25 e -194 mmol DIC m-2 day-1. Os resultados sao discutidos como indicadores de um comportamento biologico dinâmico direcionado a producao ou mineralizacao da materia orgânica no sistema. E o sistema pareceu deslocar-se de uma funcao heterotrofica para autotrofica. Em adicao, orientados pelos resultados obtidos, enfatizamos a importância axial da estrategia de amostragem e do tratamento cientifico de dados obtidos para a caracterizacao/interpretacao do metabolismo de sistemas aquaticos.","PeriodicalId":76429,"journal":{"name":"Revista brasileira de biologia","volume":"60 1","pages":"607-614"},"PeriodicalIF":0.0,"publicationDate":"2000-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1590/S0034-71082000000400010","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67177963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Hidrogeochemical comparative study of the Ja and Jacar-Guau River watersheds, So Paulo, Brazil","authors":"Á. Souza, J. Tundisi","doi":"10.1590/S0034-71082000000400004","DOIUrl":"https://doi.org/10.1590/S0034-71082000000400004","url":null,"abstract":"The main subject of the present work was to carry out a hydrochemistry comparative study between two river basins in Sao Paulo State: the Jacare-Guacu Basin (21°37'-22°22'S and 47°43'-48°57'W) and the Jau Basin (22°09'-22°28'S and 48°16'-48°47'W). Nine sampling points in the Jacare-Guacu River and eight in the Jau River were established. The water sampling was performed once each two months from March/95 to September/95. The following variables were analyzed: temperature (°C), pH, conductivity (mScm¾1), dissolved oxygen (mg/L) and ionic water composition. Comparatively the Jau River showed higher spatial variability, less oxygenated water and higher mineralization, due to high pedological and geological substrate richness, point sources existence and less riparian forests. The Jacare-Guacu River showed less spatial variability, more oxygenated water with lower ionic concentration due to the lower geological and pedological watershed richness, absence of pollution from point sources and higher riparian forest protection.","PeriodicalId":76429,"journal":{"name":"Revista brasileira de biologia","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2000-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1590/S0034-71082000000400004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67177301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
José Roberto Trigo, José Roberto Trigo, W. F. D. Santos
{"title":"Insect mortality in Spathodea campanulata Beauv. (Bignoniaceae) flowers.","authors":"José Roberto Trigo, José Roberto Trigo, W. F. D. Santos","doi":"10.1590/S0034-71082000000300019","DOIUrl":"https://doi.org/10.1590/S0034-71082000000300019","url":null,"abstract":"","PeriodicalId":76429,"journal":{"name":"Revista brasileira de biologia","volume":"60 3 1","pages":"537-8"},"PeriodicalIF":0.0,"publicationDate":"2000-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67177243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Zahorcsak, Renato Azevedo Matias Silvano, I. Sazima
{"title":"Feeding biology of a guild of benthivorous fishes in a sandy shore on south-eastern Brazilian coast.","authors":"P. Zahorcsak, Renato Azevedo Matias Silvano, I. Sazima","doi":"10.1590/S0034-71082000000300016","DOIUrl":"https://doi.org/10.1590/S0034-71082000000300016","url":null,"abstract":"The feeding biology of eight species of benthivorous fishes was studied in a sandy shore at Anchieta Island, south-eastern Brazilian coast. The fishes fed mainly on Amphipoda and Mysidacea crustaceans. The diet of the most abundant species, the drum Umbrina coroides, was analyzed in three standard length classes (20-55, 56-90 and 91-135 mm). This sciaenid showed an ontogenetic diet shift from Mysidacea to Amphipoda. The feeding behaviour of the sciaenid U. coroides and the gerreid Eucinostomus gula was recorded while snorkeling. During their foraging both species uncovered small organisms buried in the sand. Notwithstanding general similarities in diet, U. coroides and E. gula presented differences in feeding behaviour and morphology. Two carangid species of the genus Trachinotus differed in diet composition and consumed a larger array of food items than the remaining fish species. Differences in diet and feeding activity between the remaining benthivorous species were noted. These differences possibly reduce overlap in resource use and favour the coexistence of guilds of benthivorous fishes on sandy shores.","PeriodicalId":76429,"journal":{"name":"Revista brasileira de biologia","volume":"60 3 1","pages":"511-8"},"PeriodicalIF":0.0,"publicationDate":"2000-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1590/S0034-71082000000300016","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67177470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. E. Almeida, E. F. Ramos, E. Gouvêa, M. do Carmo-Silva, J. Costa
{"title":"Natural history of Ctenus medius Keyserling, 1891 (Araneae, Ctenidae) I: observations on habitats and the development of chromatic patterns.","authors":"C. E. Almeida, E. F. Ramos, E. Gouvêa, M. do Carmo-Silva, J. Costa","doi":"10.1590/S0034-71082000000300015","DOIUrl":"https://doi.org/10.1590/S0034-71082000000300015","url":null,"abstract":"Ctenus medius Keyserling, 1891 is a common species in several spots of Mata Atlântica, however there is a great lack of studies in all aspects of its natural history. This work aims to elucidate aspects of ecotope preference compared to large spiders, and to provide data on the development of chromatic patterns during its life cycle. The observations on the behavior of C. medius were done in the campus of Centro Universitário de Barra Mansa (UBM) by means of observations and nocturnal collections using cap lamps. For observations on the development of chromatic patterns, spiderlings raised in laboratory, hatched from an oviposition of a female from campus of UBM, and others spiderlings collected in field were used. The field observations indicate that: C. medius seems to prefer ecotopes characterized by dense shrub vegetation or herbal undergrowth; Lycosa erythrognatha and L. nordeskioldii seems to prefer open sites; Phoneutria nigriventer seems to prefer shrub vegetation and anthropogenic ecotopes as rubbish hills; Ancylometes sp. seems to prefer ecotopes near streams. Concerning chromatic patterns, it was observed that males and females show well distinct patterns during the last two instars, allowing distinction by sex without the use of a microscope. Through chromatic patterns it was also possible to draw a distinction between C. medius and C. ornatus longer that 3 mm cephalothorax width. 69 specimens of C. medius (males and females) collected in the campus of UBM did not show a striking polymorphism in chromatic pattern, but one among 7 adult females collected in National Park of Itatiaia, showed a distinct chromatic pattern.","PeriodicalId":76429,"journal":{"name":"Revista brasileira de biologia","volume":"60 3 1","pages":"503-9"},"PeriodicalIF":0.0,"publicationDate":"2000-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1590/S0034-71082000000300015","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67177407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Improvement of silver impregnation technique (Protargol) to obtain morphological features of protists ciliates, flagellates and opalinates","authors":"I. D. Silva-Neto","doi":"10.1590/S0034-71082000000300010","DOIUrl":"https://doi.org/10.1590/S0034-71082000000300010","url":null,"abstract":"The research on ciliates, flagelates and opalinates have been widespread by the utilization of techniques employing silver impregnation (Protargol), modified by several authors. However, these are time consuming and its results are variable. The present work is a variant of the technique described by Tuffrau (1964, 1967) showing some adaptations made in our laboratory. The organisms can be preserved by different fixatives (alcoholic Bouin, Stieve's fluid, 2.5% glutaraldehyde and others) and then rinsed in destilled water followed by a fast clarification by 3% sodium hypochloride. If the organism is very sensitive to hypochloride, 4% sodium lauryl sulfate may be used and then washed 3 times in distilled water. The protista can be adhered to the glass slides with Mayer's glycerinated-albumin (1 glycerin vol. to 1 or 2 albumin vol.), diluted in water at a proportion of 1:10 Cv/v., or with 1% polylysine followed by fast washes with distilled water. After the slide preparation, they were covered with a layer of 0,8% Silver proteinate. Right after that, the slide has to be placed in a glass tray lined with moist tissue and covered to prevent the proteinate to dry. The tray was placed in a incubator at 40o-50oC for 30 minutes. The slides are rinsed for 1 minute. with warm (35oC) distilled water. The development of the material should be done with 0.4% hydroquinone with a maximum incubation time of 1 minute. It should be developed gradually, controlling the silver impregnation intensity by observation under optical microscope. Next, rinse in distilled water for 1 minute, and then, fix in 2,5% Sodium thiosulfate. Rinse the slide for two minutes before dehydrating it in an alcoholic serial 50-100o. Finally rinse the slides in xylene. Mount the slides with Entellan MerckTM or Canada balsam.","PeriodicalId":76429,"journal":{"name":"Revista brasileira de biologia","volume":"60 1","pages":"451-459"},"PeriodicalIF":0.0,"publicationDate":"2000-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1590/S0034-71082000000300010","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67177437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Raptors and \"campo-cerrado\" bird mixed flock LED BY Cypsnagra hirundinacea (Emberizidae:Thraupinae).","authors":"J. Ragusa-Netto","doi":"10.1590/S0034-71082000000300011","DOIUrl":"https://doi.org/10.1590/S0034-71082000000300011","url":null,"abstract":"Bird mixed flocks including Cypsnagra hirundinacea and Neothraupis fasciata as species with sentinels were studied in \"campo-cerrado\" in order to investigate the possible relationship between alertness and the mixed flock leadership. This study was conducted from March to September 1996 and mixed flocks were observed on average for 2:30h. The time with sentinels were recorded for C. hirundinacea and N. fasciata. The sentinels of Cypsnagra hirundinacea performed most of the vigilance (time with sentinel was on average 42 +/- 17%, 41 +/- 17% of which by C. hirundinacea, whereas only 1.2% by N. fasciata) and gave all the alarm calls recorded (54% of the encounters with raptors stimulated alarm calls). A relationship was verified between time with sentinel and the rate of encounters with raptor (ANOVA, F = 3.0. P < 0.05). The results of this study are an evidence for the anti-predatory function of mixed flocks, in campo-cerrado, and the alertness as a major feature of a leader species, since C. hirundinacea always led those flocks.","PeriodicalId":76429,"journal":{"name":"Revista brasileira de biologia","volume":"60 3 1","pages":"461-7"},"PeriodicalIF":0.0,"publicationDate":"2000-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1590/S0034-71082000000300011","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67177487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Biological observations on Tupiperla (Plecoptera: Gripopterygidae).","authors":"C. Froehlich","doi":"10.1590/S0034-71082000000300009","DOIUrl":"https://doi.org/10.1590/S0034-71082000000300009","url":null,"abstract":"Tupiperla illiesi fly along the year in the coastal mountains of southeastern Brazil, with larger numbers during the warmer rainy season. In the seasonal tropical climate of Central Brazil and Serra do Cipó, MG, T oliveirai and T. gracilis, respectively, fly along the year but the pattern is still not known. In the higher elevation (ca. 1600 m a.s.l.) of Campos do Jordao, SP, species of Tupiperla may not fly during the colder months. Seasonal size variation, a phenomenon still little understood, occurred both in tropical and subtropical climates of the states of Goiás and São Paulo.","PeriodicalId":76429,"journal":{"name":"Revista brasileira de biologia","volume":"60 3 1","pages":"447-50"},"PeriodicalIF":0.0,"publicationDate":"2000-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1590/S0034-71082000000300009","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67176750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}