African Journal of Biochemistry Research最新文献_第2页

Profile of CA 15-3 and CEA during breast cancer chemotherapy at Ouagadougou, Burkina Faso 瓦加杜古，布基纳法索乳腺癌化疗期间ca15 -3和CEA的分析

African Journal of Biochemistry Research Pub Date : 2021-10-31 DOI: 10.5897/ajbr2021.1131

Marie Soudre Fabienne, Kiba Alice, Kouraogo Arnaud, Karfo Raoul, B. Aboubacar, Kagambega Arsène, Kabré Elie, Sakandé Jean

引用次数: 1

Phytochemical and biological investigations of extracts from the roots of Cocos nucifera L. (Arecaceae) and Carica papaya L. (Caricaceae), two plants used in traditional medicine 两种传统药用植物槟榔科(槟榔科)和番木瓜果(加勒比科)根提取物的植物化学和生物学研究

African Journal of Biochemistry Research Pub Date : 2021-07-31 DOI: 10.5897/ajbr2020.1107

W. Belem-Kabré, B. Kaboré, Adjaratou Compaoré-Coulibaly, Tata Kadiatou Traoré, Emmanuel A. M. Thiombiano, Mariam Nebié-Traoré, Moussa, Compaoré, F. Kini, S. Ouédraogo, M. Kiendrebeogo, N. Ouedraogo

{"title":"Phytochemical and biological investigations of extracts from the roots of Cocos nucifera L. (Arecaceae) and Carica papaya L. (Caricaceae), two plants used in traditional medicine","authors":"W. Belem-Kabré, B. Kaboré, Adjaratou Compaoré-Coulibaly, Tata Kadiatou Traoré, Emmanuel A. M. Thiombiano, Mariam Nebié-Traoré, Moussa, Compaoré, F. Kini, S. Ouédraogo, M. Kiendrebeogo, N. Ouedraogo","doi":"10.5897/ajbr2020.1107","DOIUrl":"https://doi.org/10.5897/ajbr2020.1107","url":null,"abstract":"and Carica papaya L. (Caricaceae) are two plants used to treat several human pathologies such as oxidative stress and inflammatory diseases. The aim of this study is to assess anti-inflammatory activity, antioxidant activity and acute oral toxicity, of aqueous and methanolic extracts from C. nucifera L. and C. papaya L. using appropriate experimental models. The acute oral toxicity test of extracts on mice was evaluated using Economic Cooperation and Development (OECD) guidelines 423. The capacity of extracts to inhibit key inflammation enzymes such as 15-lipoxygenase, phospholipase A 2 , cyclooxygenases 1 and 2 was evaluated in vitro . The antioxidant activity was assessed in vitro using four methods (inhibition of lipid peroxidation, FRAP, DPPH and ABTS tests). The phytochemical screening showed the presence of sterols and triterpenes, saponins, flavonoids, phenolics and tannins. Methanolic extract from C. nucifera exhibited higher values of 855.06 ± 1.71 mg GAE/ g, 418.22 ± 1.92 mg CE / g, 19.31 ± 0.6 mg GAE / g, 20.6 ± 0.36 mg QE / g dry extract respectively for phenolics, condensed tannins, hydrolysable tannins and flavonoid content. The extracts demonstrated antioxidant capacity, potential to inhibit pro-inflammatory enzymes and a lethal dose (LD 50 ) was estimated to 5000 mg/kg b.w. The results of this study constitute a solid scientific basis that can justify the traditional uses of these plants.","PeriodicalId":7631,"journal":{"name":"African Journal of Biochemistry Research","volume":"108 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77291490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Serum lipid profile based on the prandial state among adult subjects 基于膳食状态的成人血脂分析

African Journal of Biochemistry Research Pub Date : 2021-07-31 DOI: 10.5897/AJBR2021.1118

M. Gomina, Corinne Tchegnonsi Tognon, Tarik Salifou, Gilbert Djidonou, S. Zinsou

{"title":"Serum lipid profile based on the prandial state among adult subjects","authors":"M. Gomina, Corinne Tchegnonsi Tognon, Tarik Salifou, Gilbert Djidonou, S. Zinsou","doi":"10.5897/AJBR2021.1118","DOIUrl":"https://doi.org/10.5897/AJBR2021.1118","url":null,"abstract":"This study was aimed to access the variations in serum lipid parameters based on the prandial state of adult patients. Through a cross-sectional descriptive and analytical study conducted in Parakou (Benin), from February 1 to May 1, 2020, 100 adult subjects (average age 31.21±12.36 years) were selected with systematic census. After ethical advice, a first sample was taken on empty-bellied adult subjects. Then, three additional samples were respectively taken two hours, four hours, and six hours after these subjects had eaten a meal of their choice. Serum lipid parameters were measured through enzymatic colorimetric methods. The average values of fasting lipid parameters in g/L were: Total cholesterol (1.48±0.35), HDL cholesterol (0.49±0.14), LDL cholesterol (0.84±0.36) and triglycerides (0.63±0.34). The comparison of the fasting and postprandial lipid parameters values is presented as follow: Triglyceridemia was significantly higher 2 h (P=0.000) and 4 h after meal (P=0.001). Only one type of food consumed was associated with mixed hyperlipidemia at six hours postprandial state (P=0.022). It may therefore be concluded that consumed foods do not cause most dyslipidemias 6 h in the postprandial state. During the screening for dyslipidemias in adults, the lipid profile can therefore be measured after six hours postprandial state. \u0000 \u0000 \u0000 \u0000 Key words: Lipid profile, fasting, prandial state, adults.","PeriodicalId":7631,"journal":{"name":"African Journal of Biochemistry Research","volume":"75 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83777794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of phytochemical constituents, proximate contents and glycemic index of bambara groundnut (Vigna subterranea L. Verdc) varieties grown in Northeastern Nigeria 尼日利亚东北部班巴拉花生(Vigna subterranea L. Verdc)品种植物化学成分、近似含量和血糖指数的评价

African Journal of Biochemistry Research Pub Date : 2021-07-31 DOI: 10.5897/AJBR2021.1120

A. Mohammed, H. Mhya

{"title":"Evaluation of phytochemical constituents, proximate contents and glycemic index of bambara groundnut (Vigna subterranea L. Verdc) varieties grown in Northeastern Nigeria","authors":"A. Mohammed, H. Mhya","doi":"10.5897/AJBR2021.1120","DOIUrl":"https://doi.org/10.5897/AJBR2021.1120","url":null,"abstract":"Bambara groundnut (Vigna subterranea L. Verdc) is widely used as a source of food and also has rich fibre and nutritional values. Hence, this research aimed at determining the phytochemical, proximate content as well as glycemic index and glycemic load of four varieties of Bambara groundnut found in Alkalere area of Bauchi State northeastern Nigeria for possible nutritional and medicinal utilization. Seeds of Bambara groundnut varieties were processed by heating at 60°C and cooled then grinded into powder using a mechanical grinder. The flour was used for phytochemical and proximate analysis as well as glycemic index (GI) and glycemic load (GL) determinations. The results revealed the presence of saponins, tannins, steroids, cardiac glycosides, alkaloids and flavonoids in all the varieties while anthraquinones was not detected in all the four varieties. Proximate analysis showed high contents of carbohydrates and protein with low ash content in all the four varieties. The study also found that black seeds of Bambara groundnut had the lowest GI of 66.1 and GL at 1.33 while Brown-black seeds Bambara groundnut was the highest values in GI 75.6 and GL 1.51, respectively. The study for the first time reported the GI and GL differed among varieties with different seed color of different varieties of Bambara groundnut where a black seed variety of Bambara groundnuts possessed some good qualities for use in the nutritional management of blood glucose likely for diabetic patients. \u0000 \u0000 \u0000 \u0000 Key words: Bambara groundnut, phytochemical properties, proximate, glycemic index, glycemic load.","PeriodicalId":7631,"journal":{"name":"African Journal of Biochemistry Research","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80490599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Sphenostylis stenocarpa (Hochst. Ex A. Rich) and fermented Parkia biglobosa ((Jacq, R.B.R.) supplementation prevents high fat diet induced derangements in kidney of albino rats 狭叶菊(Hochst.)添加高脂饲料诱导的白化大鼠肾脏紊乱可预防高脂饲料诱导的白化大鼠肾脏紊乱

African Journal of Biochemistry Research Pub Date : 2021-01-31 DOI: 10.5897/AJBR2020.1099

O. Awoyinka, T. Omodara, F. C. Oladele, M. O. Alese, E. O. Odesanmi, D. D. Ajayi, G. S. Adeleye, O. Omoleye

{"title":"Sphenostylis stenocarpa (Hochst. Ex A. Rich) and fermented Parkia biglobosa ((Jacq, R.B.R.) supplementation prevents high fat diet induced derangements in kidney of albino rats","authors":"O. Awoyinka, T. Omodara, F. C. Oladele, M. O. Alese, E. O. Odesanmi, D. D. Ajayi, G. S. Adeleye, O. Omoleye","doi":"10.5897/AJBR2020.1099","DOIUrl":"https://doi.org/10.5897/AJBR2020.1099","url":null,"abstract":"Prevalence of obesity is on the increase globally with high fat diet (HFD) known to be the main contributing factor. This study was carried out to determine the actions of fermented Parkia biglobosa (Iru) and Sphenostyles stenocarpa (Otili) on the kidney of obese induced albino rats. The rats were grouped into a control group fed with normal rats chow and three different high fat diet groups (HFD1, HFD2, HFD3) mixed with different proportions of P. biglobosa and S. stenocarpa. After feeding ad-libitum for six weeks blood samples were collected to determine albumin, total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL) serum low density lipoprotein, enzymes (ALT, ALP, and AST) and Kidney histopathology. Results showed that there was a significant reduced body weight (p<0.05) in the treated rats compared with the control animals. Furthermore, the plasma lipid profiles were also improved, with a decrease in total cholesterol (TC), triglycerides (TG), low density lipoprotein (LDL) while boosting the high density lipoprotein (HDL). Similarly, histological examination revealed normal kidney with no negative changes such as dilation in blood vessels, cell infiltration, tubular defects, etc associated with taking high fat diet. In conclusion, supplementing a combination of fermented P. biglobosa (iru) and S. stenocarpa (otili) into diets show promise as a natural and safe anti-obesity agent that can ameliorate renal biochemical and histopathological changes associated with obesity. \u0000 \u0000 \u0000 \u0000 Key words: Parkia biglobosa, Sphenostyles stenocarpa, high fat diet, kidney, obesity.","PeriodicalId":7631,"journal":{"name":"African Journal of Biochemistry Research","volume":"132 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2021-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72617368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Influence of the genetic polymorphism of haptoglobin in the occurrence of retinopathy and nephropathy in diabetics subjects 接触珠蛋白基因多态性对糖尿病患者视网膜病变和肾病发生的影响

African Journal of Biochemistry Research Pub Date : 2021-01-31 DOI: 10.5897/AJBR2020.1106

Sagne RenÃ© Ngor, Djité Moustapha, Kandji Pape Matar, Diop Jean Pascal Demba, Barry Nene Oumou Kesso, Thioune Ndeye Marieme, N. H. Malick, G. Fatou, N. Rokhaya, Lopez-Sall PhilomeÌ€ne, C. Aynina, D. Amadou, Ndour-Mbaye Maimouna, Gueye Papa Madièye

{"title":"Influence of the genetic polymorphism of haptoglobin in the occurrence of retinopathy and nephropathy in diabetics subjects","authors":"Sagne RenÃ© Ngor, Djité Moustapha, Kandji Pape Matar, Diop Jean Pascal Demba, Barry Nene Oumou Kesso, Thioune Ndeye Marieme, N. H. Malick, G. Fatou, N. Rokhaya, Lopez-Sall PhilomeÌ€ne, C. Aynina, D. Amadou, Ndour-Mbaye Maimouna, Gueye Papa Madièye","doi":"10.5897/AJBR2020.1106","DOIUrl":"https://doi.org/10.5897/AJBR2020.1106","url":null,"abstract":"The objective of this study was to determine the frequency of haptoglobin (Hp) genotypes and their association with nephropathy and retinopathy in diabetic subjects. This is a case-control study conducted in diabetic subjects developing nephropathy and/or retinopathy. Each patient was matched to a control of the same sex and the same age ± 2 years. Hp genotyping was performed by conventional PCR without enzymatic digestion on the Proflex® System PCR (Biosystems, Spain) and the biochemical parameters were determined using enzymatic techniques with the Cobas c311 system (Roche Diagnostics, Switzerland). The study population consisted of 60 diabetic subjects with an average age of 56 years and a sex ratio of 0.43. The Hp2-2 genotype was more frequent in diabetic subjects (40%) compared to control subjects (28.3%) (p= 0.18). The distribution of Hp1-1 and Hp2-1 genotypes in diabetic subjects showed rates of 46.7 and 13.3% respectively. Multivariate analysis showed that the Hp 2-2 genotype was more associated with nephropathy (OR=1.77; p=0.8). These results showed an association between Hp polymorphism and diabetic microangiopathy. Thus, Hp2-2 genotype could be associated with a risk factor predisposing to the onset of diabetic kidney disease. \u0000 \u0000 \u0000 \u0000 Key words: Polymorphism, gene, haptoglobin, diabetes, nephropathy, retinopathy.","PeriodicalId":7631,"journal":{"name":"African Journal of Biochemistry Research","volume":"112 1","pages":"9-14"},"PeriodicalIF":0.0,"publicationDate":"2021-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74768719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insulin resistance and specific biomarkers in blood and urine of type 2 diabetic patients with or without nephropathy in Basrah, Iraq 伊拉克巴士拉伴有或不伴有肾病的2型糖尿病患者血液和尿液中的胰岛素抵抗和特异性生物标志物

African Journal of Biochemistry Research Pub Date : 2020-10-31 DOI: 10.5897/AJBR2020.1101

A. J. Al-Fartosy, N. A. Awad, Sadoun Abbas Alsalimi

{"title":"Insulin resistance and specific biomarkers in blood and urine of type 2 diabetic patients with or without nephropathy in Basrah, Iraq","authors":"A. J. Al-Fartosy, N. A. Awad, Sadoun Abbas Alsalimi","doi":"10.5897/AJBR2020.1101","DOIUrl":"https://doi.org/10.5897/AJBR2020.1101","url":null,"abstract":"Diabetic nephropathy (DN) is a master cause of all surplus death-rate among type 2 diabetes mellitus (T2DM) patients with microalbuminuria. This study aimed to find effective biomarkers for early predicting of DN. Present study included 63 patients with T2DM (31 patients with DN, 32 patients without DN) and 33 healthy controls. These three groups were matched for their glucose, urea, creatinine, insulin, L-Carnitine (LC), osteoprotegerin (OPG), sialic acid (SA), trace elements (Selenium, Zinc, Magnesium), albumin (Alb), and fibronectin (FN). Glucose, urea, and creatinine were determined by spectrophotometer. Insulin, LC, OPG, SA, Alb, and FN were assayed by enzyme-linked immunosorbent assay (ELISA). Insulin resistance (IR) was calculated by the Homeostatic Model Assessment for Insulin Resistance (HOMA-IR) equation. Selenium was measured by hydride generation while Zinc and Magnesium were measured by flame atomic absorption spectrometer. Compared with controls, the results indicated that T2DM patients with or without DN had a significant increase in glucose, urea, creatinine, insulin, IR, OPG, SA, Alb, FN and a significant decrease in LC and trace elements levels. It was concluded that IR is strongly associated with obesity and had an important role in the pathogenesis and increased complication of diabetes which could be used as excellent indicators for early-stage DN in T2DM patients and thus decreasing mortality and morbidity. \u0000 \u0000 \u0000 \u0000 Key words: Diabetic nephropathy, insulin resistance, obesity, oxidative stress, traces elements.","PeriodicalId":7631,"journal":{"name":"African Journal of Biochemistry Research","volume":"6 1","pages":"125-134"},"PeriodicalIF":0.0,"publicationDate":"2020-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80248782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Correlation between glycated hemoglobin and venous blood sugar in diabetic patients monitored in Abidjan 在阿比让监测糖尿病患者糖化血红蛋白和静脉血糖的相关性

African Journal of Biochemistry Research Pub Date : 2020-10-31 DOI: 10.5897/AJBR2020.1102

M. Absalome, C. Massara, K. Gervais, Diallo Issiagha, A. A. Alexandre, Yapo-Ake B en edicte, Ecrabey Yann Christian, Kouakou Francisk, Bene Yao Roger Esp erance, T. G. Georges

{"title":"Correlation between glycated hemoglobin and venous blood sugar in diabetic patients monitored in Abidjan","authors":"M. Absalome, C. Massara, K. Gervais, Diallo Issiagha, A. A. Alexandre, Yapo-Ake B en edicte, Ecrabey Yann Christian, Kouakou Francisk, Bene Yao Roger Esp erance, T. G. Georges","doi":"10.5897/AJBR2020.1102","DOIUrl":"https://doi.org/10.5897/AJBR2020.1102","url":null,"abstract":"The aim of this study was to determine the correlation between glycated hemoglobin and blood sugar levels in diabetic subjects carried out in Abidjan. This cross-sectional study included 100 patients with diabetes monitored, for three months, for whom glycated blood glucose and hemoglobin were performed, this after informed consent of the patients. Pearson and Spearman correlation tests were used, at the 5% threshold. The patients with normal HbA1C and normal blood glucose accounted for 55.34 and 32%, respectively. A sedentary lifestyle and body mass index > 25 kg/m² were associated with a significant increase in the risk of increased blood glucose and HbA1C. The presence of a complication was associated with a 3.06-fold higher risk of high glycated hemoglobin (p= 0.0073), while blood glucose was not significantly associated with the onset of complications. Glycated hemoglobin was significantly correlated with blood glucose with a correlation coefficient of 0.4412 (p 0.0001). In multivariate analysis, hyperglycemia was significantly associated with alcohol consumption and non-compliance with antidiabetic treatment. Poor compliance was significantly associated with increased glycated hemoglobin (>7%) and tobacco consumption. The glycated hemoglobin was highly correlated with blood sugar, and was found to be a better predictor of diabetes complications than glycemia. \u0000 \u0000 \u0000 \u0000 Key words: Diabetes mellitus, glycated hemoglobin, blood sugar, Abidjan, Cote d’Ivoire.","PeriodicalId":7631,"journal":{"name":"African Journal of Biochemistry Research","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78962167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of acute oral toxicity, hemato-biochemical activity and physiological responses of rabbits and rats administered Moringa oleifera leaf extract and meal 辣木叶提取物和辣木粉对家兔和大鼠急性口服毒性、血液生化活性和生理反应的评价

African Journal of Biochemistry Research Pub Date : 2020-10-31 DOI: 10.5897/ajbr2020.1077

Boni Saka Konmy Basile, A. Pascal, Doko-Allou Sanni-yo

{"title":"Evaluation of acute oral toxicity, hemato-biochemical activity and physiological responses of rabbits and rats administered Moringa oleifera leaf extract and meal","authors":"Boni Saka Konmy Basile, A. Pascal, Doko-Allou Sanni-yo","doi":"10.5897/ajbr2020.1077","DOIUrl":"https://doi.org/10.5897/ajbr2020.1077","url":null,"abstract":"The present study aims to evaluate acute oral toxicity of leaf extract of Moringa oleifera and determine the effect of the leaf’s meals of this plant on hematological and biochemical parameters of the rabbit. Ten rats weighing 150 ± 200 g were used for the oral acute toxicity study. Five rats received orally a single dose of 2000 mg/kg of weight of aqueous extract and 5 rats used as control. Forty-eight New Zealand rabbits, weighing 968±100 g were randomly spread into 4 treatments groups containing 12 growing rabbits each. Rabbits are fed rations T0, T1, T2 and T3 containing respectively 0, 5, 10 and 15% of the leaf powder M. oleifera for 56 days. The results show that M. oleifera is not toxic at 2000 mg/kg. The results of the biochemical and hematological parameters obtained in the rabbits do not show any significant difference (p > 0.05) between the treatments. These results suggest that feeding M. oleifera up to 15% inclusion in rabbit diet will not have a deleterious effect on the health of rabbits. However, histological studies of the liver and kidney would be necessary to confirm the innocuousness of the plant. \u0000 \u0000 \u0000 \u0000 Key words: Moringa oleifera, rats, rabbits, oral toxicity, hematological-biochemical parameters.","PeriodicalId":7631,"journal":{"name":"African Journal of Biochemistry Research","volume":"37 1","pages":"142-149"},"PeriodicalIF":0.0,"publicationDate":"2020-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73595114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Sub-acute and protective effect of Cymbopogon citratus against carbon tetrachloride-induced liver damage 香茅对四氯化碳所致肝损伤的亚急性及保护作用

African Journal of Biochemistry Research Pub Date : 2020-10-31 DOI: 10.5897/AJBR2019.1064

M. Oshiobugie, A. Raphael, R. Kareem

{"title":"Sub-acute and protective effect of Cymbopogon citratus against carbon tetrachloride-induced \u0000liver damage","authors":"M. Oshiobugie, A. Raphael, R. Kareem","doi":"10.5897/AJBR2019.1064","DOIUrl":"https://doi.org/10.5897/AJBR2019.1064","url":null,"abstract":"The study evaluates the sub-acute toxicity and antioxidant potential of ethanolic leaf extract of Cymbopogon citratus against CCl4-induced toxicity in Sprague Dawley rats. The ethanolic leaf extract of C. citratus was prepared by solvent maceration method. The phytochemicals present in the extract were determined using standard methods. The potential sub-acute toxicities were evaluated using OECD procedure. The sub-acute toxicity of the extract at the doses of 125, 250 and 500 mg/kg, b.wt. was administered orally for 28 days. Another sets of rats were made hepatotoxic by orally administered with CCl4 (20% CCl4 in olive oil) twice per week for a period of five weeks. They were treated with C. citratus extract (300 and 600 mg/kg body weight) once a day for 35 days. Biochemical parameters were used to assess the hepatoprotective effects of the extract on liver tissues. Phytochemical screening of C citratus shows the presence of anthraquinones, alkaloids, flavonoids, etc. The administration of C. citratus is not hematotoxic and significantly reduced (P<0.05) elevated liver biomarker enzymes, urea, creatinine and the level of malondialdehyde. Treatment with the extract was found to significantly increase (P<0.05) TP level, the activities of superoxide dismutase and catalase. Liver histopathology shows that the extract reduced the incidence of liver lesions induced by CCl4. The administration of C. citratus did not produce any toxic effects in the sub-acute study. The plant exhibits potent protective effects in CCl4-induced liver damage due to decrease in liver biomarker enzymes activities, increase of antioxidant-defense system and inhibition of lipid peroxidation. \u0000 \u0000 \u0000 \u0000 Key words: Sub-acute toxicity, protective effects, Cymbopogon citratus, carbon tetrachloride, hematological, oxidative stress parameters.","PeriodicalId":7631,"journal":{"name":"African Journal of Biochemistry Research","volume":"3 1","pages":"112-124"},"PeriodicalIF":0.0,"publicationDate":"2020-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78733408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5