{"title":"[At the background of ecological parasitology].","authors":"S I Fokin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The data on scientific and teaching activities of A. A. Filipchenko, doctor of medicine and scientist-parasitologist, one of the founders of ecological parasitology in Russia is presented in this article. His fate and participation in the formation of this biological discipline were concealed for a long time. One of the reasons for this situation is the destruction of the scientist in 1938 during the course of mass repressions performed by Soviet State against its own citizens.</p>","PeriodicalId":76305,"journal":{"name":"Parazitologiia","volume":"48 6","pages":"472-9"},"PeriodicalIF":0.0,"publicationDate":"2014-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33270024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Homoxenous trypanosomatids from true bugs Pyrrhocoris apterus (L.) in the north of the Pskov region].","authors":"A O Frolov, M N Malysheva, A Yu Kostygov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In the north of the Pskov region (58 degrees 35' N, 28 degrees 55' E) the appearance of a single colony of true bugs Pyrrhocoris apterus has been recorded. Dissection of 95 individuals from this colony revealed 100% prevalence of infection with homoxenous trypanosomatids. In 3% of the cases intestinal infection was accompanied by hyperinvasion into the salivary glands and hemolyph of the hosts. Analysis of trypanosomatid morphotypes demonstrated mixed infections in all studied P. apterus individuals. At least 4 forms of promastigotes along with epimastigotes, choanomastigotes and amastigotes were found. The distribution of the trypanosomatid morphotypes over all intestinal parts as well as salivary glands and hemolymph was investigated. Three isolates of the flagellates were deposited into the living cultures collection of the laboratory of Protozoology of the Zoological institute of the Russian Academy of Sciences.</p>","PeriodicalId":76305,"journal":{"name":"Parazitologiia","volume":"48 6","pages":"461-71"},"PeriodicalIF":0.0,"publicationDate":"2014-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33270027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Mites of the genus Schizocarpus Trouessart, 1896 (Acariformes: Chirodiscidae) from the North American beavers (Castor canadensis) in Russia.","authors":"A V Bochkov, A P Saveljev","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Four native species of parasitic mites belonging to the genus Schizocarpus Trouessart, 1896 (Acariformes: Chirodiscidae) are recorded on the North American beaver Castor canadensis Kuhl, 1820 (Rodentia: Castoridae) from Russia. Totally ten beavers from all three main geographically isolated populations of in Russia (Leningrad Province, Voronezh Biosphere Reserve (beaver farm) and Khabarovsk Territory) were examined. Additionally, in captivity (Voronezh beaver farm) eight species were recorded switched from the Eurasian beaver Castor fiber Linnaeus, 1758 on C. canadensis.</p>","PeriodicalId":76305,"journal":{"name":"Parazitologiia","volume":"48 6","pages":"430-6"},"PeriodicalIF":0.0,"publicationDate":"2014-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33270567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Contribution to the black fly fauna of the tribes Nevermanniini and Wilhelmiini (Diptera: Simuliidae) of the Sakha Republik (Yakutia)].","authors":"S V Aibulatov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In this paper, the data on fauna of Nevermanniini and Wilhelmiini tribes (Diptera: Simuliidae) were analyzed. All the accessible literature sources, institute collections and new materials recently collected in Yakutia were examined. Regional fauna was supplemented by two species: Schoenbaueria dendrofila (Patrusheva, 1962) and S. furculata Shewell, 1952, the latter being new for the Russian fauna also. The distribution of five more species from Simuliidae family was clarified. Processing of the collected material proved that 10 species previously registered in Yakutia can still be found in the region.</p>","PeriodicalId":76305,"journal":{"name":"Parazitologiia","volume":"48 6","pages":"409-22"},"PeriodicalIF":0.0,"publicationDate":"2014-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33270565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[The analysis of climatic and biological parameters for the pest spread risk modelling of the wood nematode species Bursaphelenchus spp. and Devibursaphelenchus teratospicularis (Rhabditida: Aphelenchoidea)].","authors":"A Y Ryss, M V Mokrousov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Based on the forest woody species wilt areassurvey in Nizhniy Novgorod region in August 2014, the possible factors of the pest spread risk modelling were analysed on six species of the genus Bursaphelenchus and Devibursaphelenchus teratospicularis using six parameters: plant host species, beetle vector species, average temperatures in July and January, annual precipitation. It was concluded that these parameters in the evaluated wilt spots correspond to climatic and biological data of the already published woody plants wilt records in Europe and Asia caused by the same nematode pest species. It was speculated that the annual precipitation of 600 mm and average July temperature of 25 degrees C or higher, are the critical combination that may be used to develop the predicative risk modelling in the forests' and parks' wilt monitoring.</p>","PeriodicalId":76305,"journal":{"name":"Parazitologiia","volume":"48 6","pages":"454-60"},"PeriodicalIF":0.0,"publicationDate":"2014-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33270022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Early stages of development of Trypanosoma rotatorium (Mayer, 1843) from peripheral blood and internal organs of Anurans Bufo bufo (Linnaeus) and Rana sp. (Anura)].","authors":"M N Malysheva","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The data on the fauna of trypanosomes of Anura of the Leningrad Province are given. The initial development stages of Trypanosoma rotatorium in peripheral blood and internal organs of the frog are described for the first time.</p>","PeriodicalId":76305,"journal":{"name":"Parazitologiia","volume":"48 5","pages":"373-81"},"PeriodicalIF":0.0,"publicationDate":"2014-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33264660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Goussia obstinata sp. n. (Sporozoa: Eimeriidae), a new coccidian species from intestines of the Amur sleeper Perccottus Glenii Dybowski, 1877 (Perciformes: odontobutidae)].","authors":"S G Sokolov, A Ja Moshu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Goussia obstinata sp. n. is described from the intestine epithelium of the Amur sleeper Perccottus glenii Dybowski, 1877 from Russia and Moldova. The species was examined in an optical microscope. Merogonic, gamogonic and sporogonic stages of the species are located in cells of the gut epithelium. Sporulation is endogenous; oocysts are released in the gut lumen. Non-sporulated oocysts are spherical or ellipsoidal; sporulated oocysts are rounded, 6.7-11.4 μm in diameter, with a colorless, single-layer, very fine and easily ruptured wall. Micropyle and oocyst residuum is absent; 1-2 small polar granules of 1.0-2.5 μm are sometimes present. Oocysts contain four compact widely oval, slightly narrow-ended sporocysts. The sheath of the sporocyst is formed of two folds divided by a slightly S-shaped longitudinal suture. Coarsely granulated, globular or oval compact sporocyst's residuumis located between sporozoites. Sausage-shaped sporozoits are subdivided by a bend into two unequal parts, being 6.4-9.8 μm (long part) and 2.6-4.8 μm (short part) long, arranged in a top-to-tail position. Our preliminary data suggests that infestation of the sleeper with the examined parasite is not associated with the morbidity and mortality of the fish. A new combination Goussia marmorata (Molnár, 1996) comb. n. is proposed for a species originally described in the content of the genus Eimeria Schneider, 1875 from the Western tubenose goby Proterorhinus semilunaris.</p>","PeriodicalId":76305,"journal":{"name":"Parazitologiia","volume":"48 5","pages":"382-92"},"PeriodicalIF":0.0,"publicationDate":"2014-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33264661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S A Timofeev, I V Sendersky, O A Pavlova, V V Dolgikh
{"title":"[Peculiarities of the expression, structure, and localization of the subtilisin-like protease in the microsporidium Paranosema locustae].","authors":"S A Timofeev, I V Sendersky, O A Pavlova, V V Dolgikh","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Peculiarities of the expression, localization, and structure of the subtilisin-like protease from the microsporidium Paranosema locustae, a parasite of the migratory locust and other orthopteran species, are analyzed. Heterologous expression of the microsporidian ferment in the bacterium Escherichia coli allowed obtaining antibodies to the recombinant protein and to start its examination. In spite of the presence of the N-tail signal peptide in the ferment, potentially able to secret it into the cytoplasm of the infected cell, immunoblotting with obtained antibodies had demonstrated specific accumulation of the protease in the insoluble fraction of spore homogenate. At the same time, the ferment was absent in intracellular stages.of the parasite and also in the cytoplasm of infested host cells. Accumulation of mRNA, coding the studied protein in microsporidian spores was confirmed with the use of RT-PCR method. Heterologous expression of the protease in the methylotrophic yeast Pichiapastoris demonstrated the same result. The ferment of P. locustae was not secreted into a culture medium and was absent in the cytoplasm of yeast cells, accumulating in a dissoluble (membrane) fraction of the homogenate. On the whole, the obtained data testify to the fact that the subtilisin-like protease of P. locustae plays an important role in the physiology of spores rather than participates in host-parasite relations during intra-cellular development.</p>","PeriodicalId":76305,"journal":{"name":"Parazitologiia","volume":"48 5","pages":"337-47"},"PeriodicalIF":0.0,"publicationDate":"2014-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33265301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Infestation of lower crustaceans (Copepoda, Cladocera) with microsporidians (Microsporidia) in Western Siberia].","authors":"V V Lukyantsev, A V Simakova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The search for intermediate hosts of microsporidians of bloodsucking mosquitoes of the family Culicidae with complicated two-host developmental cycles in Western Siberia resulted in revealing of 19 microsporidian species in crustaceans. Crustacean microsporidians are represented as by specialized parasites of crustacean, being or being not related to microsporidians parasitizing mosquitoes, and by parasites of mosquitoes having only a part of their complicate life cycle in crustaceans. Sequencing of ssrDNA of microsporidins from copepods had demonstrated that Acanthocyclops venustus Norman et Scott can be an intermediate host of Amblyospora rugosa Simakova et Pankova, 2005 .from mosquitoes Oc. cataphylla Dyar., and Acantocyclops reductus (Chappuis) can be an intermediate host of Trichoctosporea pygopellita Larsson, 1994, a parasite of the mosquito Oc. excrucians (Walker). According to their fine structure, microsporidians from Daphnia Muller belong to the genera Bervaldia Larsson, 1981 and Agglomerata Larrson et Yan, 1988. The infestation rate in natural population of crustaceans was low, constituting about 2%. The maximal infestation rate was observed in temporary reservoirs since late April till early May.</p>","PeriodicalId":76305,"journal":{"name":"Parazitologiia","volume":"48 5","pages":"358-72"},"PeriodicalIF":0.0,"publicationDate":"2014-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33265303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}