American journal of physiology. Regulatory, integrative and comparative physiology最新文献

{"title":"Sleep tight with vitamin D's might.","authors":"John J Durocher, Ezra Mutai","doi":"10.1152/ajpregu.00058.2025","DOIUrl":"10.1152/ajpregu.00058.2025","url":null,"abstract":"","PeriodicalId":7630,"journal":{"name":"American journal of physiology. Regulatory, integrative and comparative physiology","volume":" ","pages":"R557-R558"},"PeriodicalIF":2.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Follow the fat. Regulation of metabolic substrates in trout.","authors":"Grant B McClelland, Sulayman A Lyons","doi":"10.1152/ajpregu.00060.2025","DOIUrl":"10.1152/ajpregu.00060.2025","url":null,"abstract":"","PeriodicalId":7630,"journal":{"name":"American journal of physiology. Regulatory, integrative and comparative physiology","volume":" ","pages":"R559-R561"},"PeriodicalIF":2.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heat exposure, heat strain, and off-work recovery of Guatemalan sugarcane workers.","authors":"Lyndsay Krisher, Karely Villarreal Hernandez, Yaqiang Li, Jaime Butler-Dawson, Diana Jaramillo, Hillary A Yoder, Kevin E Miller, Evan Johnson, Katherine A James, Miranda Dally, Elizabeth J Carlton, Daniel Pilloni, Alex Cruz, Joshua Schaeffer, John L Adgate, Lee S Newman","doi":"10.1152/ajpregu.00004.2025","DOIUrl":"https://doi.org/10.1152/ajpregu.00004.2025","url":null,"abstract":"<p><p><b>Background:</b> Agricultural workers are at high risk for heat related illnesses when performing heavy labor in hot conditions. Occupational heat strain, the physiological response to heat stress, is hypothesized to be common in this worker population but has rarely been measured objectively through core body temperature (Tc). The objective of this study was two-fold 1) evaluate workday heat strain and 2) examine the trajectory of heat exposure and Tc from the workday through the off-work hours to advance understanding of the recovery process and conditions of heat-exposed agricultural workers. <b>Methods:</b> Among 55 male Guatemalan agricultural workers, individual heat exposure (using ambient temperature loggers) and Tc (via an ingestible pill) were measured across a 24-hour period including workday and off-work hours. Urine samples were collected to assess hydration status on and off-work. Workers reported off-work activities, hydration practices, sleep, and nutrition through a survey. Data were summarized using descriptive statistics and visualizations. <b>Results:</b> Workers experienced excessive heat strain (44% with Tc > 38.0 °C, 16% with Tc > 38.3 °C, and 6% with Tc > 38.5 °C) during the workday. Approximately 29% achieved a higher maximal Tc during off-work hours than during the workday. Nearly 15% of workers reported sleeping < 7 hours. <b>Discussion:</b> There is a need to understand off-work conditions, practices, and resources available to workers to mitigate heat strain and related illnesses. Heat stress and Tc monitoring should extend to post-work shift for assessment of workers' physiological recovery and to inform more comprehensive interventions to protect worker health.</p>","PeriodicalId":7630,"journal":{"name":"American journal of physiology. Regulatory, integrative and comparative physiology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cardiac Autonomic Function in Young, Healthy Adults: Influence of Race and Sex.","authors":"Ziba Taherzadeh, Claire E Kissell, Benjamin E Young, Taha Ali Alhalimi, Brandi Y Stephens, Jasdeep Kaur, Yungfei Kao, R Matthew Brothers, Paul J Fadel","doi":"10.1152/ajpregu.00288.2024","DOIUrl":"https://doi.org/10.1152/ajpregu.00288.2024","url":null,"abstract":"<p><p>Non-Hispanic Black (BL) adults living in the U.S. are more likely to develop cardiovascular disease (CVD) compared to their Non-Hispanic White (WH) counterparts. While measures of cardiac autonomic function [i.e., spontaneous cardiac baroreflex sensitivity (BRS), heart rate variability (HRV)] have a predictive value for CVD, studies investigating racial differences in cardiac autonomic function are limited and have reported conflicting results. Furthermore, sex differences are often not considered despite BL women having a high prevalence of CVD. We hypothesized that young BL men and women would exhibit lower cardiac BRS and HRV compared to their WH counterparts. Heart rate and beat-to-beat blood pressure were continuously recorded during 5 minutes of supine rest in 145 young (18-33 years), healthy, BL (37 men, 38 women) and WH (39 men, 31 women) adults to assess cardiac BRS and HRV. Overall cardiac BRS (sequence method) was higher in BL adults compared to WH adults (p < 0.001), which was mainly driven by differences between BL and WH men (BL men: 34±16 vs. WH men: 21±9 ms/mmHg, p < 0.001) compared to women (BL women: 27±12 vs. WH women: 24±11 ms/mmHg; p > 0.05). Likewise greater HRV in BL adults, indexed by root mean square of successive differences (RMSSD), was primarily driven by BL men (BL men: 109±59 vs. WH men: 64±33 ms; p < 0.001) rather than BL women (p > 0.05). Thus, contrary to our hypothesis, these results support that reduced cardiac autonomic function does not manifest early in life among young BL adults.</p>","PeriodicalId":7630,"journal":{"name":"American journal of physiology. Regulatory, integrative and comparative physiology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143787518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nutritional modulation of the mTORC1 pathway in muscle: differential effect according to muscle and sex.","authors":"C Breuillard, S Le Plénier, C Guihenneuc, C Choisy, C Hourdé, Christophe Moinard","doi":"10.1152/ajpregu.00156.2024","DOIUrl":"https://doi.org/10.1152/ajpregu.00156.2024","url":null,"abstract":"<p><p>Numerous studies have focused on nutrient-driven regulation of muscle metabolism/homeostasis through the mTORC1 pathway, but their results fail to converge, perhaps due to differences in mTORC1 pathway protein studied, muscle type and/or sex. The aim of this work was to study the influence of these factors on mTORC1 pathway activation in response to food intake. Rats (16 male and 16 female) were fasted for 20 hours then were randomized into two groups: a postabsorptive group in which the animals were euthanized in the fasted state, and a postprandial group in which the animals were euthanized 30 min after food intake (10 g). Plasma glucose, insulin and amino acids were assayed. Muscles (<i>extensor digitorum longus, tibialis, soleus, gastrocnemius and plantaris</i>) were removed and Western blotted to analyze activation of the mTORC1 pathway (phosphorylation of Akt, 4EBP1, S6K1). Levels of Akt, 4EBP1 and S6K1 activation were compared between muscles and by sex in different nutritional states, and a Kruskal-Wallis test was performed to find statistically significant differences. Food intake led to an increase in plasma concentrations of glucose, insulin and total amino acids (p<0.0001). Levels of Akt, 4EBP1 and S6K1 activation differed significantly between muscles and by sex and nutritional state. Different phosphorylation sites in the same muscle were not correlated with each other. These results suggest that mTORC1 activation level is sensitive to muscle type, sex and nutritional state. Studies on this signal transduction pathway therefore require an individualized approach, considering all the factors that may affect it.</p>","PeriodicalId":7630,"journal":{"name":"American journal of physiology. Regulatory, integrative and comparative physiology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143784473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanisms involved in cardiovascular and hydroelectrolytic changes in dehydrated high-fat-diet-fed rats.","authors":"Jéssica Matheus Sá, Marcos Vinícius Fernandes, Roberto Braz Pontes, Eduardo Colombari, José Vanderlei Menani, Débora Simões Almeida Colombari","doi":"10.1152/ajpregu.00171.2024","DOIUrl":"10.1152/ajpregu.00171.2024","url":null,"abstract":"<p><p>Obesity is increasingly prevalent worldwide, and climate change is exacerbating water shortages, leading to dehydration. Both obesity and dehydration cause increased arterial pressure (AP), fluid electrolytic imbalance, and neuroinflammation. Thus, the present study aimed to verify the changes in the cardiovascular system, hydroelectrolytic balance, and microglia and neuronal activation in rats fed with a high-fat diet (HFD) in response to 24 h of water deprivation (WD) and the possible mechanisms involved. Male Holtzman rats (290-310 g) were fed with a standard diet (SD, 10% calories from fat) or HFD (46% calories from fat) for 6 wk before the WD experiments. Compared with WD SD rats, WD HFD rats presented a greater c-Fos immunolabeling in the subfornical organ (SFO) and supraoptic nucleus and greater microglial activation in SFO. WD-induced water intake was lower in HFD rats than in SD rats. WD HFD rats presented greater antidiuresis and lesser natriuresis than WD SD rats. Renal denervation did not change the antidiuresis or natriuresis observed in WD HFD- or SD-fed rats. The lower water intake in WD HFD rats might be due to neuroinflammation and/or decreased urinary output. The increase in AP after WD was similar between HFD and SD, but it is more dependent on angiotensin II type 1 (AT1) receptor activation in HFD rats. Overall, HFD rats seem less responsive to fluid and electrolyte balance responses to WD, highlighting the need for strategies to prevent dehydration in individuals with obesity, particularly during rising drought conditions worldwide.<b>NEW & NOTEWORTHY</b> Obesity and dehydration are common worldwide. Our study with an animal model found that changes in arterial pressure are linked to increased activation of the AT1 receptor in obese, dehydrated rats. The renal nerves appear unrelated to the significant decrease in urinary volume and sodium excretion in these animals. Neuroinflammation and reduced urine output may explain their lower water intake. These findings highlight the need for strategies to prevent dehydration in individuals with obesity.</p>","PeriodicalId":7630,"journal":{"name":"American journal of physiology. Regulatory, integrative and comparative physiology","volume":" ","pages":"R481-R491"},"PeriodicalIF":2.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of successive sets of high-intensity leg press on cerebral hemodynamics across menstrual cycle phases.","authors":"Elric Y Allison, Matin Borhani, Alysha C D'Souza, Huseyn A Ismayilov, Brandan Wilson, Yixue Mei, Patrice Brassard, Stuart M Phillips, Baraa K Al-Khazraji","doi":"10.1152/ajpregu.00257.2024","DOIUrl":"10.1152/ajpregu.00257.2024","url":null,"abstract":"<p><p>This study examined how successive sets of high-intensity leg press (LP) resistance exercise impact the cerebral pressure-flow relationship in untrained males and eumenorrheic females not taking oral contraceptives and assessed how the menstrual cycle (MC) phase influences the cerebral pressure-flow relationship and cerebral hemodynamics (middle cerebral artery blood velocity, MCAv; via transcranial Doppler ultrasound) during and after LP exercise in females. Young adults (11M;11F) performed three sets of leg-press exercises at 90% of their one-repetition maximum. Data from males and females in the early follicular phase were pooled together. Directional sensitivity of the cerebral pressure-flow relationship was calculated as the ratio of the rate of changes in MCAv and mean arterial pressure (MAP) (ΔMCAv_T/ΔMAP_T) per transition between eccentric and concentric muscular contractions during each repetition of LP exercise. ΔMCAv_T/ΔMAP_T was higher during concentric than eccentric phases (<i>P</i> < 0.001) in males and early follicular (EF) phase in females. There were no effects of successive leg press sets on any systemic or cerebral hemodynamic measures. The MC phase affected directional sensitivity and cerebral hemodynamics, with greater responses in the mid-luteal (ML) phase than the EF phase. We observed a MAP direction by MC phase interaction on relative directional sensitivity, with greater sensitivity during concentric contractions in the ML phase (<i>P</i> = 0.02). Our results suggest that successive sets of LP exercises do not impact the cerebral pressure-flow relationship or cerebral hemodynamics during and immediately following LP exercise. The MC phase appears to influence the cerebral pressure-flow relationship and cerebral hemodynamics both during and following LP exercise, mediated by vasoprotective effects of increased estrogen concentration in the ML phase compared with the EF phase.<b>NEW & NOTEWORTHY</b> Successive sets of high-intensity bilateral leg press exercises do not appear to affect cerebral or systemic hemodynamic measures, given adequate recovery time. The menstrual cycle phase impacts the directional sensitivity of the cerebral pressure-flow relationship during high-intensity bilateral leg press exercises. During high-intensity bilateral leg press exercises, the cerebrovasculature appears to be more pressure passive in the mid-luteal phase of the menstrual cycle.</p>","PeriodicalId":7630,"journal":{"name":"American journal of physiology. Regulatory, integrative and comparative physiology","volume":" ","pages":"R447-R459"},"PeriodicalIF":2.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143063153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the link between sleep patterns and early cardiovascular disease markers in young adults with chronic anxiety.","authors":"Jacob W Richardson, Emily A Buck, Jennifer B Weggen, Brad T Bakken, Brandon J Fitzpatrick, Raven G Campbell, Ryan S Garten","doi":"10.1152/ajpregu.00271.2024","DOIUrl":"10.1152/ajpregu.00271.2024","url":null,"abstract":"<p><p>Chronic anxiety is commonly associated with poor sleep patterns, which may contribute to an increased risk of cardiovascular disease (CVD) through mechanisms like oxidative stress, vascular dysfunction, and poor blood pressure control. As sleep disturbances, particularly poor sleep quality and/or regularity, have been independently linked to CVD development, this study explored whether sleep quality/regularity in young adults with chronic anxiety is associated with early indicators of CVD risk, specifically oxidative stress, vascular function, and blood pressure control. Twenty-eight young (24 ± 4 yr) participants with a prior clinical diagnosis of generalized anxiety disorder (GAD) or elevated GAD symptoms (GAD-7 > 10) had their sleep quality [total sleep time (TST) and sleep efficiency (SE)] and regularity [via TST/SE standard deviations (SD)] assessed for seven consecutive days. Various precursors to CVD development such as oxidative stress, brachial artery function, microvascular function, and blood pressure control [exercise pressor responses and cardiovagal baroreflex sensitivity (cBRS)] were also evaluated. Pearson's correlations were used to determine potential relationships between sleep quality/regularity and CVD precursors. Both sleep irregularity variables [SE-SD (<i>r</i> = 0.61; <i>P</i> < 0.01) and TST-SD (<i>r</i> = 0.39; <i>P</i> = 0.04)], but neither of the sleep quality variables, were positively correlated with oxidative stress. TST-SD alone was significantly associated with lower brachial artery function (<i>r</i> = -0.44; <i>P</i> = 0.02) and cBRS (<i>r</i> = 0.43; <i>P</i> = 0.02), with TST-SD median splits further highlighting these differences. The study found that irregular TST in individuals with chronic anxiety was significantly associated with higher oxidative stress, lower brachial artery function, and blunted blood pressure control (lower cBRS), key precursors of CVD.<b>NEW & NOTEWORTHY</b> This study examined the relationship between sleep irregularity and early cardiovascular disease (CVD) precursors in young adults with chronic anxiety. Key findings revealed that irregular total sleep time, rather than overall sleep quality, was significantly associated with greater oxidative stress, lower brachial artery function, and diminished blood pressure control. These results suggest that sleep irregularity in individuals with chronic anxiety may play a critical role in the development of CVD in this population.</p>","PeriodicalId":7630,"journal":{"name":"American journal of physiology. Regulatory, integrative and comparative physiology","volume":" ","pages":"R494-R505"},"PeriodicalIF":2.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143613030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of dietary supplements on core temperature and sweating responses in hot environmental conditions: a meta-analysis and meta-regression.","authors":"Jennifer S Peel, Melitta A McNarry, Shane M Heffernan, Venturino R Nevola, Liam P Kilduff, Mark Waldron","doi":"10.1152/ajpregu.00186.2024","DOIUrl":"10.1152/ajpregu.00186.2024","url":null,"abstract":"<p><p>Dietary supplements are widely used among individuals exposed to hot environments, but whether their consumption confers any thermoregulatory effect is unclear. Therefore, we systematically evaluated the effect of dietary supplementation on key aspects of thermoregulation [core temperature (T_core) and sweating responses] in the heat. Three databases were searched in April 2024. After screening, 124 peer-reviewed articles were identified for inclusion within three separate meta-analyses: <i>1</i>) peak T_core; <i>2</i>) whole body sweat rate (WBSR); <i>3</i>) local sweat rate (LSR). The moderating effect of several variables (e.g., training and heat acclimation status), known to influence thermoregulatory function, were assessed via subanalysis and meta-regression. There was no overall effect of the differing supplement types on WBSR (<i>P</i> = 0.405) and LSR (<i>P</i> = 0.769), despite taurine significantly increasing WBSR (<i>n</i> = 3, Hedges' <i>g</i> = 0.79, <i>P</i> = 0.006). Peak T_core was significantly affected by supplement type (<i>P</i> = 0.011), primarily due to caffeine's \"small\" significant positive effect (<i>n</i> = 30; Hedges' <i>g</i> = 0.44, <i>P</i> < 0.001) and taurine's (<i>n</i> = 3, Hedges' <i>g</i> = -0.66, <i>P</i> = 0.043) and oligonol's (<i>n</i> = 3; Hedges' <i>g</i> = -0.50, <i>P</i> = 0.014) \"medium\" significant negative effects. Dietary supplements, such as amino acids (e.g., taurine), some antioxidants and anti-inflammatories (e.g., oligonol) conferred the greatest thermoregulatory benefits during heat exposure. Taurine ingestion in such conditions may lower heat strain, which is likely through its augmentation of thermal sweating. Conversely, caffeine intake may potentially pose the greatest risk in the heat due to its effect on T_core.<b>NEW & NOTEWORTHY</b> The effects of dietary supplements on core temperature and sweating responses when ingested in the heat varied greatly. Some supplements demonstrated the potential to improve thermoregulatory capacity (e.g., select amino acids, anti-oxidants and anti-inflammatories), while others had no or even deleterious effects on thermal balance (e.g., caffeine). These findings have implications for those ingesting dietary supplements for their health and/or performance effects during exposure to hot environmental conditions. Certain supplements should possibly be avoided in the heat, while others may elicit a thermoregulatory benefit.</p>","PeriodicalId":7630,"journal":{"name":"American journal of physiology. Regulatory, integrative and comparative physiology","volume":" ","pages":"R515-R555"},"PeriodicalIF":2.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143063169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knockdown of the type 1 cannabinoid receptor in the central amygdala increases both spontaneous and water deprivation-induced sodium intake in rats.","authors":"S G Ruginsk, M P Greenwood, M Greenwood, L L K Elias, D Murphy, J Antunes-Rodrigues","doi":"10.1152/ajpregu.00241.2024","DOIUrl":"10.1152/ajpregu.00241.2024","url":null,"abstract":"<p><p>Important inputs originating in the forebrain circumventricular organs and also in the central amygdala (CeA) trigger essential water deprivation (WD)-induced behaviors, such as thirst and sodium appetite. Together with the secretion of the neurohypophysial peptides arginine vasopressin (AVP) and oxytocin (OT), these behavioral responses seek to maintain the normalcy of extracellular fluid (ECF) osmolality and volume. Within this context, the main hypothesis tested by the present study was that CeA type 1 cannabinoid receptors (CB1Rs) were essential for the maintenance of body fluid homeostasis, particularly in response to WD challenge. We found that CeA CB1R knockdown increased spontaneous and WD-induced hypertonic saline intake, without significantly impacting water ingestion. In euhydrated rats, despite unaltered urinary volume, CB1R knockdown reduced urinary osmolality, and diminished urinary nitrate concentrations, suggesting reduced renal sodium excretion. No relevant changes were induced by CeA CB1R knockdown on urinary parameters following WD-induced rehydration, which is consistent with unaltered AVP and OT mRNA transcription and hormone release under the same experimental conditions. Taken together, the present data support the notion that CeA CB1Rs participate in both spontaneous and WD-induced NaCl intake, without significantly affecting neuroendocrine output. Given the well-described facilitatory CeA role on natriorexigenic responses, and the reported interplay between CB1Rs and γ-aminobutyric acid (GABA) within the CeA, the present findings suggest that CB1Rs may indirectly regulate sodium appetite through effects on CeA GABAergic neurotransmission.<b>NEW & NOTEWORTHY</b> CeA CB1R knockdown increased spontaneous and WD-induced hypertonic saline intake, without significantly impacting water ingestion. In euhydrated rats, despite unaltered urinary volume, CB1R knockdown reduced urinary osmolality, and diminished urinary nitrate concentrations, suggesting reduced renal sodium excretion. No relevant changes were induced by CeA CB1R knockdown on urinary parameters following WD-induced rehydration, which is consistent with unaltered AVP and OT mRNA transcription and hormone release under the same experimental conditions.</p>","PeriodicalId":7630,"journal":{"name":"American journal of physiology. Regulatory, integrative and comparative physiology","volume":" ","pages":"R423-R432"},"PeriodicalIF":2.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143447613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}