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Mikrobiologicheskii zhurnal最新文献

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[The destruction of diethylene glycol by a Pseudomonas putida BS-2 culture]. [恶臭假单胞菌BS-2培养物对二甘醇的破坏]。
Mikrobiologicheskii zhurnal Pub Date : 1992-01-01
S A Sedina
{"title":"[The destruction of diethylene glycol by a Pseudomonas putida BS-2 culture].","authors":"S A Sedina","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"54 1","pages":"61-7"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12751057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[An evaluation of the biochemical typing of Klebsiella cultures]. [克雷伯菌培养物的生化分型评价]。
Mikrobiologicheskii zhurnal Pub Date : 1992-01-01
S I Pokhil, L A Kosobutskiĭ
{"title":"[An evaluation of the biochemical typing of Klebsiella cultures].","authors":"S I Pokhil,&nbsp;L A Kosobutskiĭ","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The paper deals with the results of analysis of 219 strains of Klebsiella using 12 tests--fermentation of adonite, asparaginic acid, sodium citrate, dulcite, d-tartrate, glutamic acid, inosite, L-proline, sodium malonate; reactions with methyl red, Foges-Proscauer, with 5-ASA. A scheme for determining fermentovars of Klebsiella is suggested which includes the tests--fermentation of adonite, dulcite, d-tartrate, glutamic acid; color reaction with 5-ASA. The groups of Klebsiella different in origin are characterized by nonhomogeneous distribution of different fermentovars. The suggested method of biochemical labelling may be one of the basic ones in complex typing of Klebsiella.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"54 1","pages":"3-9"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12751784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[RNAse substrate specificity in Acholeplasma laidlawii PG-8]. [RNAse底物特异性]。
Mikrobiologicheskii zhurnal Pub Date : 1992-01-01
Z Ia Ianchinova, D P Grama, I G Skripal'
{"title":"[RNAse substrate specificity in Acholeplasma laidlawii PG-8].","authors":"Z Ia Ianchinova,&nbsp;D P Grama,&nbsp;I G Skripal'","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A substrate specificity of RNAses of A. laidlawii PG-8 to polynucleotides - poly (C), poly (U), poly (A) has been studied. Due to the data obtained both intracellular and extracellular RNAses of A. laidlawii possess similar specificity to different polynucleotides. Both RNAses preferentially break cytidine-bonds. Specificity of the studied enzymes in respect to polyuridylic and polyadenylic acids was less expressed.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"54 1","pages":"58-61"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12751056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The determination of the location of the antigens of the causative agent of melioidosis at the ultrastructural level by using monoclonal antibodies]. [用单克隆抗体测定类鼻疽病病原抗原在超微结构水平的定位]。
Mikrobiologicheskii zhurnal Pub Date : 1992-01-01
V I Kapliev, N N Piven', N P Khrapova
{"title":"[The determination of the location of the antigens of the causative agent of melioidosis at the ultrastructural level by using monoclonal antibodies].","authors":"V I Kapliev,&nbsp;N N Piven',&nbsp;N P Khrapova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The indirect immunoperoxidase method with the use of monoclonal antibodies has been employed to determine the localization of antigens of melioidosis agents. The formed reaction products were then detected by the electron microscopic analysis. The most important in melioidosis pathogenesis antigens 6 and 8 (AG 6 and AG 8) were determined to localize on the cell wall and in the capsule-like mucous layer, respectively. Monoclonal antibodies to AG 8 due to their marked cross-linking properties promote stabilization, preservation and detection of labile melioidosis agent. At the same time the mucous layer prevents monoclonal antibodies to AG 6 to access to the corresponding antigen, localized on the cell wall and makes its detection difficult.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"54 1","pages":"85-9"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12546993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The chromatographic properties of the DNA-dependent DNA polymerases from Acholeplasma laidlawii PG-8]. [laidlawii PG-8中DNA依赖性DNA聚合酶的色谱性质]。
Mikrobiologicheskii zhurnal Pub Date : 1992-01-01
S V Bezuglyĭ, I G Skripal', V V Babichev
{"title":"[The chromatographic properties of the DNA-dependent DNA polymerases from Acholeplasma laidlawii PG-8].","authors":"S V Bezuglyĭ,&nbsp;I G Skripal',&nbsp;V V Babichev","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The DNA-dependent DNA-polymerase (DNA polymerase I which is not sorbed on the column with DEAE-cellulose, and DNA-polymerase II, which is absorbed by this column and is eluted from it by 0.3 M of NaCl), have been isolated from Acholeplasma laidlawii PG-8. DNA-polymerase I in homogeneous state was obtained as a result of the stepwise treatment by heparin-sepharose (elution at 0.35 M of NaCl) and poly-U-sepharose (elution at 0.3 M of NaCl). It was presented on the electrophoregram by one polypeptide with molecular weight of 72 kDalton. The second form of DNA polymerase was also obtained in homogeneous state as a result of sequential treatment on heparin-sepharose (elution at 0.3 M of NaCl) and on poly-A-sepharose (elution at 0.25 M of NaCl): the protein which had manifested polymerase activity was a polypeptide with molecular weight of 45 kDalton.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"54 1","pages":"51-7"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12751055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The bacterial destruction of glycols]. [细菌对乙醇的破坏]。
Mikrobiologicheskii zhurnal Pub Date : 1992-01-01
S A Sedina, V N Ivanov, V S Podgorskiĭ
{"title":"[The bacterial destruction of glycols].","authors":"S A Sedina,&nbsp;V N Ivanov,&nbsp;V S Podgorskiĭ","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"54 1","pages":"96-112"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12751060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The use of a CHO cell culture in studying Vibrio cholerae grown under different conditions]. [CHO细胞培养在研究不同条件下生长的霍乱弧菌中的应用]。
Mikrobiologicheskii zhurnal Pub Date : 1991-11-01
O I Sal'nikova, V V Lobanov, N Ia Shimaniuk
{"title":"[The use of a CHO cell culture in studying Vibrio cholerae grown under different conditions].","authors":"O I Sal'nikova,&nbsp;V V Lobanov,&nbsp;N Ia Shimaniuk","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Studies of biological activity of cholera vibrios in cultures of chinese hamster ovary cells (CHO) have revealed their strong dependence on culture conditions. Elongation of CHO cells is caused only by choleragenic strains. Under stationary conditions of culture the vibrios were found to release haemolisin into the medium and had a cytotoxic effect. Most of cytotoxic supernatants exhibited a neuraminidase activity. Proteolytic activity was less dependent on the vibrio culture conditions. Strains with a high proteolytic activity caused rounding of the CHO cells.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"53 6","pages":"94-8"},"PeriodicalIF":0.0,"publicationDate":"1991-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12889851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[A trial of cultivating Rickettsiella phytoseiuli on the SM IMV-72 medium used for growing phytopathogenic mycoplasmas]. [在用于培养植物致病性支原体的SM IMV-72培养基上培养植性立克次体的试验]。
Mikrobiologicheskii zhurnal Pub Date : 1991-11-01
G Su'taková, Z Sekejová, J Rechacek, I G Skripal', L P Malinovskaia
{"title":"[A trial of cultivating Rickettsiella phytoseiuli on the SM IMV-72 medium used for growing phytopathogenic mycoplasmas].","authors":"G Su'taková,&nbsp;Z Sekejová,&nbsp;J Rechacek,&nbsp;I G Skripal',&nbsp;L P Malinovskaia","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The work presents data on growing Rickettsiella phytoseiuli in the SM IMV-72 medium which is used for cultivation of phytopathogenic mycoplasmas. Rickettsiella was observed till the 66th day in the primary cultures and till the 16th day after the first passage. Binary division of the cells has been found only in the primary cultures; a complex cycle of the Rickettsiella development ceased at the stage of formation of the crystal-forming cells. Attempts to passivate Rickettsiella were failure.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"53 6","pages":"57-61"},"PeriodicalIF":0.0,"publicationDate":"1991-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12980472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The level of spontaneous phage production and sensitivity to melioidosis phages of museum cultures of Pseudomonas pseudomallei]. [假单胞菌博物馆培养物的自发噬菌体产生水平及对类鼻疽噬菌体的敏感性]。
Mikrobiologicheskii zhurnal Pub Date : 1991-11-01
I I Denisov, V I Kapliev
{"title":"[The level of spontaneous phage production and sensitivity to melioidosis phages of museum cultures of Pseudomonas pseudomallei].","authors":"I I Denisov,&nbsp;V I Kapliev","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The phage-producing activity and sensitivity of museum cultures of a melioidosis agent to melioidosis phages have been studied. Most cultures show spontaneous phage-production, though its level differs in strains. Some of the melioidosis cultures demonstrate lack of phages. The fact that these strains have different sensitivity to chloroform lysate of cultures producing phages indicates their possible lysogenic state. Electron microscopy has shown the presence of at least tow morphological types of phages in P. pseudomallei C-141. These data confirm existence of polysogeny in the melioidosis agent.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"53 6","pages":"66-70"},"PeriodicalIF":0.0,"publicationDate":"1991-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12889848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The bacteriocin typing of Klebsiella pneumoniae strains]. [肺炎克雷伯菌菌素分型]。
Mikrobiologicheskii zhurnal Pub Date : 1991-11-01
S I Pokhil, L A Kosobutskiĭ, B M Ovcharenko, L G Fedorova, N G Liman
{"title":"[The bacteriocin typing of Klebsiella pneumoniae strains].","authors":"S I Pokhil,&nbsp;L A Kosobutskiĭ,&nbsp;B M Ovcharenko,&nbsp;L G Fedorova,&nbsp;N G Liman","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Results of bacteriocin typing of 196 strains of the Klebsiella genus are presented. They are typified by their sensitivity to bacteriocins and by their production using colicinogenic and indicating strains from collection of P. Fredericq [correction of Frederick], D. G. Kudlaĭ, N. I. Koshanova as well as klebocinogenic K-type cultures of Klebsiella previously suggested by the authors. Investigation results have shown sufficient stability of a bacteriocinotype of the cultures confirmed by the population analysis. It is concluded that bacteriocin typing may be recommended as an additional method in epidemiological labelling of Klebsiella cultures.</p>","PeriodicalId":76162,"journal":{"name":"Mikrobiologicheskii zhurnal","volume":"53 6","pages":"88-93"},"PeriodicalIF":0.0,"publicationDate":"1991-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12982720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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