Journal of clinical & laboratory immunology最新文献_第2页

An analysis of the value of some antigen-antibody interactions used as diagnostic indicators in a treponemal Western blot (TWB) test for syphilis. 梅毒螺旋体免疫印迹(TWB)检测中一些抗原-抗体相互作用作为诊断指标的价值分析。

Journal of clinical & laboratory immunology Pub Date : 1998-01-01

R George, V Pope, M Fears, B Morrill, S Larsen

{"title":"An analysis of the value of some antigen-antibody interactions used as diagnostic indicators in a treponemal Western blot (TWB) test for syphilis.","authors":"R George, V Pope, M Fears, B Morrill, S Larsen","doi":"","DOIUrl":"","url":null,"abstract":"Densiometric quantitation and spreadsheet normalization were used to refine the parameters defining a treponemal Western blot (TWB) test for syphilis. Initially using 84 defined reactive and 105 defined non-reactive sera, we determined that the immune response to the 17 kDa antigen was the most critical of the following three candidate test determinants: the 47 kDa, 17 kDa and 15.5 kDa bands. In a second study using 124 cases of clinically diagnosed syphilis and 354 \"normal\" donors, a diluted serum sample was included as a minimal reactive control for the 17-kDa immune response. Reactivity to all three test determinants was obligatory for a test result to be interpreted as positive. Of the 124 cases of syphilis, 7 were nonreactive by TWB (sensitivity = 94%); of the 354 normal donors, 7 tested reactive (specificity = 98%). Forty (11%) normal serum samples had detectable but less than minimal reactivity to the 17 kDa band. Frequencies of immune response to a larger group of 12 antigens were tallied for the 124 clinically diagnosed cases of syphilis and an equal subset (124) of the normal group. In the normal subset, 72% and 52% of the samples had detectable reactivity to the 47 and 15.5 kDa antigens, respectively, while 10%, 5% and 3% reacted with the 17, 24 and 44.5 kDa antigens, respectively. Follow-up TWB testing of the clinically diagnosed cases revealed that previously untreated patients with primary or secondary syphilis were more likely to a show decrease in TWB reactivity than patients with latent symptoms who had been treated previously. As a diagnostic indicator of syphilis, the 17-kDa antigen was found to have the best combined attributes of sensitivity and specificity. Although, the highly specific 44.5 kDal and 24 kDal bands were often redundant as diagnostic indicators they are useful for the interpretation of borderline results. In addition, absence of the highly sensitive 47 and 15.5 kDa indicators should be useful in resolving some problem diagnoses.","PeriodicalId":75994,"journal":{"name":"Journal of clinical & laboratory immunology","volume":"50 1","pages":"27-44"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21061522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of lymphocyte subpopulations in the peripheral blood of patients with infectious mononucleosis and human immunodeficiency virus infection: a preliminary report. 传染性单核细胞增多症和人类免疫缺陷病毒感染患者外周血淋巴细胞亚群的比较:初步报告。

Journal of clinical & laboratory immunology Pub Date : 1998-01-01

S Zidovec, Z Culig, J Begovac, T Jeren

{"title":"Comparison of lymphocyte subpopulations in the peripheral blood of patients with infectious mononucleosis and human immunodeficiency virus infection: a preliminary report.","authors":"S Zidovec, Z Culig, J Begovac, T Jeren","doi":"","DOIUrl":"","url":null,"abstract":"The aim of this study was to analyse the lymphocyte subpopulations in the peripheral blood of HIV-1-infected adults to compare them with four patients with acute EBV infection. Lymphocyte subsets in 15 healthy controls, 40 HIV-1-infected adults and 4 EBV-infected patients with infectious mononucleosis were analysed by flow cytometry. The immunophenotyping of HIV-1-infected patients in different stages of disease showed a significant reduction in the percentage and absolute count of CD4+ T-lymphocytes, significantly increased percentage of CD8+ T-lymphocytes, inverted CD4/CD8 ratio and an increase in the expression of activation marker HLA-DR compared to controls. The immuno-phenotyping profiles of HIV and EBV infection share some similarities as they both result in the decreased percentage of CD4+ T-lymphocytes, increased CD8+ T-lymphocytes and an inverted CD4/CD8 ratio. Patients with HIV infection could be distinguished from patients with EBV infection by the absolute lymphocytosis and increased expression of HLA-DR seen in the patients with infectious mononucleosis. In conclusion, both HIV-1 and EBV profoundly change the distribution of lymphocyte subpopulations in the peripheral blood. It is our opinion that flow cytometry could be an aid in the rapid distinguishing of patients with suspected primary HIV-1 infection from those with infectious mononucleosis (before serology data are available).","PeriodicalId":75994,"journal":{"name":"Journal of clinical & laboratory immunology","volume":"50 2","pages":"63-9"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21334833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modulation of IL-12 by transforming growth factor-beta (TGF-beta) in Mycobacterium tuberculosis-infected mononuclear phagocytes and in patients with active tuberculosis. 转化生长因子- β (tgf - β)在结核分枝杆菌感染的单核吞噬细胞和活动性结核患者中对IL-12的调节

Journal of clinical & laboratory immunology Pub Date : 1997-01-01

Z Toossi, M Mincek, E Seeholtzer, S A Fulton, B D Hamilton, C S Hirsch

{"title":"Modulation of IL-12 by transforming growth factor-beta (TGF-beta) in Mycobacterium tuberculosis-infected mononuclear phagocytes and in patients with active tuberculosis.","authors":"Z Toossi, M Mincek, E Seeholtzer, S A Fulton, B D Hamilton, C S Hirsch","doi":"","DOIUrl":"","url":null,"abstract":"In humans, tuberculosis is associated with suppression of T-cell responses to antigens of Mycobacterium tuberculosis. Recently, the macrophage product, transforming growth factor-beta (TGF-beta) has been implicated in suppression of T-cell proliferation and cytokine production during tuberculosis. We studied the effect of TGF-beta on production of IL-12, and on the augmentation of M. tuberculosis-induced IFN gamma production by IL-12, in patients with pulmonary tuberculosis and by M. tuberculosis. Induction of IL-12 p35, but not IL-12 p40, by M. tuberculosis in monocytes was dependent on prior priming of the cells with IFN gamma. Expression of both IL-12 p40 and p35, however, was suppressed by TGF-beta. Further, TGF-beta interfered with the bioactivity of IL-12 in the enhancement of M. tuberculosis-induced IFN gamma mRNA expression and cytokine production. However, in mononuclear cells from patients with tuberculosis the main effect of TGF-beta on IL-12 appeared to be counter action to IL-12 induced IFN gamma production in response to M. tuberculosis.","PeriodicalId":75994,"journal":{"name":"Journal of clinical & laboratory immunology","volume":"49 2","pages":"59-75"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20731246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of the I-E(d)--restricted peptide recognized by an anti-idiotypic CD4+ T cell line. 抗独特型CD4+ T细胞系识别的I-E(d)-限制性肽的表征

Journal of clinical & laboratory immunology Pub Date : 1997-01-01

H Masaki, S Yamane, K Irimajiri, A Horiuchi, J Yamaguchi, R Suzuki, I Kurane

{"title":"Characterization of the I-E(d)--restricted peptide recognized by an anti-idiotypic CD4+ T cell line.","authors":"H Masaki, S Yamane, K Irimajiri, A Horiuchi, J Yamaguchi, R Suzuki, I Kurane","doi":"","DOIUrl":"","url":null,"abstract":"We have previously reported a CD3+ CD4+ CD8- T cell line, J-2R which specifically recognized J558 individual idiotope (IdI) of anti-alpha (1-->3) dextran antibodies in an I-E(d) restricted manner. The J-2R proliferated in response to J558 IdI-derived peptides; however, the ability of the peptides to evoke the proliferation of J-2R was different. In the present study, we investigated the interaction between J558 IdI-derived peptides and I-E(d) molecules in competition experiments using a M104E IdI-derived peptide, M88-105. The M88-105 inhibited the proliferation of J-2R induced by J558 IdI-derived peptides. Furthermore, the proliferation induced by the peptides J92-109 and J96-105 was inhibited by the M88-105 at much lower inhibitor/antigenic peptide ratios, compared to the proliferation induced by the J88-105. Thus, shift of the framework to C-terminus and deletion of N-terminus amino acid residues from the 18-mer peptide J88-105 made the peptides more susceptible to the inhibition by the M88-105. Sequencing of the J-2R T cell receptor (TcR) revealed that J-2R used TcR, V alpha 1, J alpha 44; V beta 15, D beta 1, J beta 1.5. These results suggest that the peptides, J88-105, J92-109 and J96-105, directly bind to I-E(d) molecules, and that the capacity of J558 IdI-derived peptides to activate J-2R depends on the affinity to the I-E(d) molecules.","PeriodicalId":75994,"journal":{"name":"Journal of clinical & laboratory immunology","volume":"49 1","pages":"1-14"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20731284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenotypic analysis of PBMCs from uterine cervix cancer patients with high natural killer activity. 高自然杀伤活性子宫癌患者外周血单核细胞表型分析。

Journal of clinical & laboratory immunology Pub Date : 1997-01-01

M I Pinel, V Pires, R C Harab, V M Rumjanek

引用次数: 0

Common variable immunodeficiency following Epstein-Barr virus infection. 爱泼斯坦-巴尔病毒感染后常见的变异性免疫缺陷。

Journal of clinical & laboratory immunology Pub Date : 1997-01-01

G Zuccaro, S Della Bella, B Polizzi, M Vanoli, R Scorza

引用次数: 0

The effects of alpha 2-antiplasmin complex and alpha 2-antiplasmin on the secretion of IgG and IgM by cultured human mononuclear cells. α 2-抗纤溶蛋白复合物和α 2-抗纤溶蛋白对培养的人单核细胞分泌IgG和IgM的影响。

Journal of clinical & laboratory immunology Pub Date : 1997-01-01

S G Zhabin, V S Gorin

引用次数: 0

CD8 lymphocytes in HIV infection: helpful and harmful. CD8淋巴细胞在HIV感染中的利弊。

Journal of clinical & laboratory immunology Pub Date : 1997-01-01

G Famularo, S Moretti, S Marcellini, E Nucera, C De Simone

{"title":"CD8 lymphocytes in HIV infection: helpful and harmful.","authors":"G Famularo, S Moretti, S Marcellini, E Nucera, C De Simone","doi":"","DOIUrl":"","url":null,"abstract":"The part played by CD8 lymphocytes in the pathogenesis of human immunodeficiency virus infection (HIV) is much disputed and the relevant issue of the controversy ranges as to whether the functional activity of these cells is beneficial or detrimental to the host. Even though CD8 cells could efficiently suppress HIV replication through both major histocompatibility complex (MHC)-restricted cytotoxic killing of infected cells, particularly during primary infection, and HIV-suppressing soluble factors, there is evidence that tissue-infiltrating CD8 lymphocytes mediate injury in several organs of HIV-infected subjects. Furthermore, CD8 lymphocytes could contribute to the destruction of CD4 cells in vivo. Of note, the virus has the capability to escape the recognition by cytotoxic CD8 cells and the cytotoxic activity of CD8 cells and their counts decline with evolving HIV infection. Several mechanisms are proposed to explain this latter finding, including the direct in vivo infection of CD8 cells by the virus. It is likely that early during the course of HIV infection when viral loads are generally low an efficient CD8 cell response can control HIV replication whereas in subjects with evolving disease, who have very high viral loads, CD8 lymphocytes remove essential components of the immune response and mediate tissue injury.","PeriodicalId":75994,"journal":{"name":"Journal of clinical & laboratory immunology","volume":"49 1","pages":"15-32"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20731285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combination of immunoadsorption therapy and high-dose methylprednisolone in patients with lupus nephritis; possible indications in patients with early stage. 免疫吸附联合大剂量甲基强的松龙治疗狼疮性肾炎早期患者可能的适应症。

Journal of clinical & laboratory immunology Pub Date : 1997-01-01

M Funauchi, S Ikoma, A Imada, A Kanamaru

{"title":"Combination of immunoadsorption therapy and high-dose methylprednisolone in patients with lupus nephritis; possible indications in patients with early stage.","authors":"M Funauchi, S Ikoma, A Imada, A Kanamaru","doi":"","DOIUrl":"","url":null,"abstract":"Objective: Therapeutic protocol and indication of immunoadsorption therapy (IA) for lupus nephritis (LN) have not been established, although it has been reported to be effective in resistant cases. Here, we performed IA and double filtration plasmapheresis (DFPP) in combination with high-dose methyprednisolone in patients with LN, and studied possible indications of IA.Methods: IA and DFPP were performed in 9 patients each with LN. They were immediately followed by intravenous infusion of 500 mg of methylprednisolone for prevention of rebound phenomenon. After these treatments 1-2 times a week, a total of 4-6 times, clinical findings were observed for 6 months.Results: The effects on clinical findings such as erythema, fever and arthralgia, serum complement activity, mean urinary protein and reduction of dose of adrenocorticosteroids were comparable in both treatments. Serum titers of ADNA decreased by IA more than DFPP (16% in IA, 38% in DFPP in 3 months), while serum immunoglobulins decreased by IA less than DFPP. Responses in urinary protein after IA tended to be better in patients with high titer of serum ADNA and without nephrotic syndrome, and not associated with disease activity of SLE.Conclusion: Removal of ADNA was more selective in IA than in DFPP, and the effects of IA were comparable with those of DFPP. Since patients with low titers of serum ADNA and nephrotic syndrome showed poor responses to IA, it might be worth trying rather in patients with early phase of lupus nephritis.","PeriodicalId":75994,"journal":{"name":"Journal of clinical & laboratory immunology","volume":"49 2","pages":"47-57"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20731245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of age on neutrophil function and its relevance to bacterial infections in the elderly. 年龄对老年中性粒细胞功能的影响及其与细菌感染的关系。

Journal of clinical & laboratory immunology Pub Date : 1997-01-01

P Angelis, S Scharf, N Christophidis

{"title":"Effects of age on neutrophil function and its relevance to bacterial infections in the elderly.","authors":"P Angelis, S Scharf, N Christophidis","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To determine the effect of age on neutrophil function in patients with and without acute bacterial infections.Method: Four groups of patients were recruited: Group 1: 15 elderly patients with infection (mean age 80.4 +/- 1.9 years), Group 2: 15 elderly control patients without infection (mean age 81.1 +/- 2.2 years), Group 3: 8 young patients with infections (mean age 26.7 +/- 2.9 years) and Group 4: 23 young controls (mean age 27.6 +/- 0.9 years). The main outcome measures included neutrophil counts and respiratory burst activation as measured by luminol enhanced chemiluminescence.Results: Mean neutrophil counts were significantly higher in young patients with infections (5.04 +/- 0.96 x 10(9)/L) compared with young controls (2.63 +/- 0.33 x 10(9)/L) (p = 0.008) and in elderly with infections (6.51 +/- 0.97 x 10(9)/L) compared with elderly controls (4.1 +/- 0.88 x 10(9)/L) (p = 0.046). There was no significant difference between neutrophil counts of old and young patients (p = 0.40) or controls (p = 0.16). Mean peak luminol chemiluminescence was significantly increased in young patients (3329 +/- 284 mV) compared with young controls (1398 +/- 108 mV) and in elderly patients (2994 +/- 219 mV) compared with elderly controls (1674 +/- 197 mV) (p < 0.001). There was no significant difference between chemiluminescence activities of young and elderly controls (p = 0.41) or young and elderly patients (p = 0.14).Conclusion: Age is not associated with a change in neutrophil number or activity in the absence of bacterial infection. Infection in both young and elderly produces a significant increase in neutrophil number and chemiluminescence activity.","PeriodicalId":75994,"journal":{"name":"Journal of clinical & laboratory immunology","volume":"49 1","pages":"33-40"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20731286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0