American Journal of Potato Research最新文献

{"title":"Evaluating the Plant based Volatile 2E-hexenal for the Sustainable Management of Pink Rot in Stored Potatoes","authors":"Phillip S. Wharton,&nbsp;Katie L. Malek,&nbsp;Alan Malek","doi":"10.1007/s12230-025-10018-6","DOIUrl":"10.1007/s12230-025-10018-6","url":null,"abstract":"<div><p><i>Phytophthora erythroseptica</i>, the causal agent of pink rot, is a significant threat to potato production, leading to yield losses and postharvest decay. Conventional management strategies rely on fungicides like phosphorous acid, but increasing consumer demand for reduced fungicide inputs and alternatives necessitates new control methods. This study evaluates the efficacy of 2<i>E</i>-hexenal, a naturally occurring volatile organic compound, in inhibiting <i>P. erythroseptica in vitro</i> and in storage. In vitro experiments demonstrated that 2<i>E</i>-hexenal was fungitoxic and completely inhibited pathogen growth at a minimum concentration of 5 µL/L, while lower concentrations exhibited fungistatic effects. Large-scale storage trials were conducted between 2016 and 2023 to assess the fumigant potential of 2<i>E</i>-hexenal on infected tubers. Initial trials showed no significant reductions in disease incidence and severity. However, subsequent experiments demonstrated that 2<i>E</i>-hexenal treatments significantly reduced disease progression. In the 2020 season-long storage trial, disease incidence in untreated tubers reached 100%, whereas treated tubers maintained up to 75% lower disease incidence rates. The 2023 trial confirmed these findings, with 2<i>E</i>-hexenal reducing disease severity by an average of 75% compared to untreated controls. These results suggest that 2<i>E</i>-hexenal is a promising alternative for managing pink rot in stored potatoes. Its ability to reduce disease incidence and severity over extended storage periods highlights its potential for integration into postharvest disease management strategies. Further research is needed to optimize application methods and confirm efficacy across different storage conditions.</p></div>","PeriodicalId":7596,"journal":{"name":"American Journal of Potato Research","volume":"103 1","pages":"45 - 58"},"PeriodicalIF":1.8,"publicationDate":"2025-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12230-025-10018-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147341430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanotechnology Applications for Enhancing Potato Productivity, Stress Resilience, and Post-Harvest Quality","authors":"Ramasubramanian Sabarivasan,&nbsp;Selvam Christinal Infanta,&nbsp;Kanagaraj Srimathi,&nbsp;Greeshma Varghese,&nbsp;R Jeevanraj,&nbsp;Shanmugam Gokul","doi":"10.1007/s12230-025-10035-5","DOIUrl":"10.1007/s12230-025-10035-5","url":null,"abstract":"<div><p>Potato (<i>Solanum tuberosum</i> L.) is the third most significant food crop in the world. Still, its yield is limited by climate change, soil erosion, ineffective use of agrochemicals, and multifaceted biotic and abiotic stresses. Nanotechnology, with its unique physicochemical characteristics, is becoming a groundbreaking technology for sustainable crop management. However, a comprehensive synthesis on its role in potato cultivation linking productivity, stress resilience, and post-harvest management remains limited, emphasizing the need for this review. This review synthesizes the up-to-date developments in nanotechnology in potato production, such as nanofertilizers, nano-fungicides, nano-insecticides, and post-harvest quality improvement. Evidence indicates that nanofertilizers improve nutrient uptake and tuber quality, while nanopesticides offer targeted, eco-friendly crop protection. Post-harvest applications, such as nanomaterials for sprout suppression, smart packaging, and nanosensors, extend shelf life and maintain tuber quality. Although these results are promising, they have not been widely adopted because of the lack of field-level validation, biosafety and environmental risks, high production costs, and unclear regulatory frameworks. Coordinated research, farmer-focused extension, and policies to facilitate its safe, effective, and sustainable integration into potato-based agri-food systems, contributing to food security and the UN Sustainable Development Goals.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7596,"journal":{"name":"American Journal of Potato Research","volume":"103 1","pages":"17 - 33"},"PeriodicalIF":1.8,"publicationDate":"2025-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147339799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Induction of Tolerance to Cryogenic Stress in Solanum tuberosum by Hydrogen Peroxide Mediated by Enzymatic Antioxidant Activity","authors":"Diana Roció Ruiz-Sáenz,&nbsp;Humberto Antonio López-Delgado,&nbsp;Diana Daniela Ayala Hernández,&nbsp;Martha Elena Mora-Herrera,&nbsp;Carlos Trejo,&nbsp;Ebandro Uscanga Mortera,&nbsp;Román Flores López","doi":"10.1007/s12230-025-10031-9","DOIUrl":"10.1007/s12230-025-10031-9","url":null,"abstract":"<div><p>Cryopreservation allows preservation of germplasm for long periods. Cryogenics involves oxidative stress. Hydrogen peroxide as a signal, is involved in tolerance to stress factors. The objective was to evaluate the effect of hydrogen peroxide on the induction of tolerance to cryogenic stress and the relationship with antioxidant enzymes. Pre-treatments with hydrogen peroxide for one hour one month before cryogenics, induced significant tolerance to cryogeny. Tolerance was associated with changes in the enzymatic antioxidants catalase and peroxidase. Induction of tolerance could be mediated by an increase in hydrogen peroxide internal concentration before cryogenics, suggesting the signaling role of this molecule in the long term. This work demonstrates the potential use of hydrogen peroxide in the culture phase prior to cryogenics, inducing long-term effects on enhancement of survival, reduction in catalase activity, increase in hydrogen peroxide content, and a higher water potential before cryogenics.</p></div>","PeriodicalId":7596,"journal":{"name":"American Journal of Potato Research","volume":"103 2","pages":"176 - 187"},"PeriodicalIF":1.8,"publicationDate":"2025-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147735065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Economic Assessment of Potato Response to Struvite and Conventional Phosphorus Fertilizer in Eastern Canada","authors":"M. Khakbazan,&nbsp;J. Nyiraneza,&nbsp;R. Benjannet,&nbsp;A. N. Cambouris,&nbsp;K. Fuller,&nbsp;S. Hann,&nbsp;N. Ziadi,&nbsp;D. Biswas","doi":"10.1007/s12230-025-10030-w","DOIUrl":"10.1007/s12230-025-10030-w","url":null,"abstract":"<div><p>Phosphorus (P) fertilizers are essential but potentially inefficient, especially in shallowrooted potatoes. A 3-year field experiment (2016-2018) was conducted in four distinct sites in Eastern Canada to investigate economic and environmental impacts of struvite-based slow-release and conventional P fertilizers in potato (<i>Solanum tuberosum</i> L.). Marginal return (MR = gross revenue-total operating cost) was assessed for different P treatments (0, 60, 120, 180, and 240 kg P₂O₅ ha^-1) from triple super phosphate (TSP) and for different mixtures of struvite (25%, 50%, and 75%) and TSP at 180 P₂O₅ kg ha^-1. Comparable MRs to TSP at a rate of 180 kg P2O5 ha-¹ were obtained with a mixture of 75% TSP and 25% struvite across sites and years. Results demonstrated that a struvite-based mixture can be a viable alternative to conventional P fertilizers, improving profitability and lowering potential cumulative environmental costs associated with P application by $8-$24 ha^-1 year^-1 for potato production.</p></div>","PeriodicalId":7596,"journal":{"name":"American Journal of Potato Research","volume":"103 2","pages":"164 - 175"},"PeriodicalIF":1.8,"publicationDate":"2025-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12230-025-10030-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147735059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temporal Epidemics of Potato Late Blight (Phytophthora Infestans) in Major Potato Growing Areas of Ethiopia","authors":"Beka Biri,&nbsp;Chemeda Fininsa,&nbsp;Habtamu Terefe,&nbsp;Zelalem Bekeko,&nbsp;Abdi Mohammed","doi":"10.1007/s12230-025-10026-6","DOIUrl":"10.1007/s12230-025-10026-6","url":null,"abstract":"<div><p>Potato late blight, caused by the oomycete Phytophthora infestans, is a catastrophic disease worldwide and causes an estimated potato yield loss of about 30–100% in Ethiopia. The research was conducted at Haramaya, Negele Arsi, and Holeta during the 2022 and 2023 cropping seasons to analyze the dynamics of potato late blight epidemics under natural infection. Four potato varieties (Gudene, Jalene, Belete, and Bubu) were arranged in a randomized complete block design with three replications. Disease progress was assessed using percentage of severity index (PSI), area under disease progress curve (AUDPC), relative AUDPC (rAUDPC) and disease progress rate. Significant differences (P &lt; 0.001) were observed among varieties. The variety Gudene and Bubu consistently exhibited lower PSI values at Haramaya, while the variety Gudene recorded the lowest PSI (22.5%), AUDPC (549.07% - days), and disease progress rate ((0.097 units day^˗1) at Holeta. In contrast, Jalene showed the highest rAUDPC values, reaching 40.11% in 2022 and 37.67% in 2023 at Haramaya, and constantly suffered maximum disease severity up to 65.2%. At Negele Arsi, variety Belete and Gudene displayed lower PSI, disease progress rates, and AUDPC values. Across all locations the variety Gudene exhibited the lowest potato late blight severity, whereas variety Jalene was the most susceptible. Based on late blight severity assessment and tuber yield measured, it is recommended that farmers in Haramaya should cultivate Gudene and Bubu, while those in Negele Arsi and Holeta consider Belete and Gudene varieties to reduce potato late blight epidemics and increase potato yield.</p></div>","PeriodicalId":7596,"journal":{"name":"American Journal of Potato Research","volume":"103 1","pages":"95 - 107"},"PeriodicalIF":1.8,"publicationDate":"2025-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147336465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Remote Sensing and Machine Learning for Irrigation Management in Potato Cultivation: A Systematic Review","authors":"Ravinder Singh,&nbsp;Lakesh Sharma,&nbsp;Hardeep Singh,&nbsp;Lincoln Zotarelli","doi":"10.1007/s12230-025-10029-3","DOIUrl":"10.1007/s12230-025-10029-3","url":null,"abstract":"&lt;div&gt;&lt;p&gt;Irrigation efficiency is vital for sustaining potato productivity under increasing climate variability and global water scarcity. As a shallow-rooted, water-sensitive crop, potato requires precise irrigation scheduling to optimize yield and tuber quality. Recent advances in remote sensing and machine learning (ML) have enabled high-resolution monitoring of crop water status, offering promising tools for automated irrigation management. This systematic review synthesizes research from 49 peer-reviewed studies (2000–2025), identified through Scopus and Web of Science using PRISMA guidelines. The findings highlight a growing use of UAV-based multispectral and thermal imaging to estimate evapotranspiration, soil moisture, and canopy temperature. ML algorithms, particularly random forest (RF), support vector machine (SVM), and partial least squares regression (PLSR), have been used to model crop water needs. However, gaps remain in the application of UAV-based hyperspectral imaging, root-zone moisture modeling, and accurate crop coefficient (K&lt;sub&gt;c&lt;/sub&gt;) estimation. Common challenges include cloud interference, limited field-scale validation, and poor system interoperability. To achieve scalable and site-specific irrigation in potato systems, future research should prioritize sensor fusion, robust model development across diverse environments, and user-focused decision support tools. Addressing these gaps is essential for advancing sustainable irrigation strategies tailored to potato’s unique water requirements. &lt;/p&gt;&lt;p&gt;Resumen La eficiencia del riego es vital para mantener la productividad de la papa bajo la creciente variabilidad climática y la escasez mundial de agua. Como cultivo de raíces superficiales y sensible al agua, la papa requiere una programación de riego precisa para optimizar el rendimiento y la calidad de los tubérculos. Los avances recientes en teledetección y aprendizaje automático (ML) han permitido el monitoreo de alta resolución del estado del agua de los cultivos, ofreciendo herramientas prometedoras para la gestión automatizada del riego. Esta revisión sistemática sintetiza la investigación de 49 estudios revisados por pares (2000-2025), identificados a través de Scopus y red de ciencias (Web of Science) utilizando las pautas PRISMA. Los hallazgos destacan un uso creciente de imágenes térmicas y multiespectrales basadas en UAV para estimar la evapotranspiración, la humedad del suelo y la temperatura del dosel. Los algoritmos de ML, en particular los bosques aleatorios, las máquinas de vectores de soporte y la regresión de mínimos cuadrados parciales, se han utilizado para modelar las necesidades de agua de los cultivos. Sin embargo, siguen existiendo lagunas en la aplicación de imágenes hiperespectrales basadas en UAV, el modelado de la humedad de la zona radical y la estimación precisa del coeficiente de cultivo (Kc). Los desafíos comunes incluyen interferencia en la nube, validación limitada a escala de campo y poca ","PeriodicalId":7596,"journal":{"name":"American Journal of Potato Research","volume":"103 1","pages":"1 - 16"},"PeriodicalIF":1.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147335882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Supplemental Application of Potassium Acetate Increased Yield of Mesa Russet Potatoes in Sandy Loam Soil with High Potassium Levels","authors":"Salah M. Abdelsalam,&nbsp;Samuel YC Essah,&nbsp;Jessica G. Davis","doi":"10.1007/s12230-025-10022-w","DOIUrl":"10.1007/s12230-025-10022-w","url":null,"abstract":"<div><p>Potato responses to potassium (K) fertilizer have sometimes been reported in high K soils. This study was designed to investigate the effects of supplemental application of K acetate, a new potassium fertilizer source, to KCl, on the production of Mesa Russet potatoes at the San Luis Valley of Colorado, USA. Treatments included (1) the control treatment where no K fertilizer was applied, (2) application of KCl pre-plant (KCl PP), (3) application of K acetate pre-plant (KAc PP), (4) foliar application of K acetate (KAc F), (5) K acetate pre-plant and foliar (KAc PP + F), and (6) foliar application of K acetate twice (KAc 2 F). KAc PP and KAc F treatments increased total tuber yield and marketable tuber yield (&gt; 114 g) compared to KCl PP treatment in both years. Measurements of tuber quality (tuber dry weight, specific gravity, cracks, knobs, and hollow heart) and plant growth (tuber bulking, aboveground biomass, stem number, and tuber number) showed no consistent treatment impacts. Supplemental application of K acetate (pre-plant or foliar) improved tuber yield of Mesa Russet potatoes; however, the mechanism through which K acetate achieved these yield gains remains unclear and warrants additional research.</p></div>","PeriodicalId":7596,"journal":{"name":"American Journal of Potato Research","volume":"103 3","pages":"277 - 289"},"PeriodicalIF":1.8,"publicationDate":"2025-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12230-025-10022-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148237256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Potato Bruises as Influenced by Cultivar, Impact Height, and Holding Temperature","authors":"R. L. Hendricks,&nbsp;N. Olsen,&nbsp;M. Thornton,&nbsp;P. Hatzenbuehler","doi":"10.1007/s12230-025-10027-5","DOIUrl":"10.1007/s12230-025-10027-5","url":null,"abstract":"<div><p>Handling potatoes at harvest and throughout transloading operations can result in two main impact injuries: blackspot and shatter bruises. The objective of this study was to examine the development of blackspot and shatter bruises over a 24 to 48-h period as influenced by cultivar, impact height and holding temperature with the intent to identify whether more rapid, on-farm bruise evaluations could be used to facilitate change in harvest management. Tubers (8.9 C pulp temperature) were bruised using a free-falling 100 g weight set at a height of 18 or 30 cm (Russet Burbank and Ranger Russet) and 30 cm for Russet Norkotah to deliver two uniform impacts on each tuber end. Impacted tubers were held at either 21.1 C or 8.9 C and evaluated after 1, 2, 3, 4, 5, 24 and/or 48 h. Peeled tubers were evaluated for incidence, color intensity, and depth of blackspot bruise and shatter bruise incidence. The change in flesh color at the impact site from pink to brown primarily occurred within one to three hours after impact, and as a result the incidence of pink discoloration declined rapidly after that time. Over 70% of the total blackspot bruise incidence was observed after four hours, depending on the impact force and cultivar. Likewise, 70% of the total bruise depth observed tended to develop within five hours after impact. The bud end had lower overall blackspot bruise incidence, color intensity and depth and was slower to develop discoloration compared to the stem end. Time after impact was not a major component to shatter bruise development. Rapid bruise assessment can be conducted between two to five hours for Russet Burbank, two to three hours for Russet Norkotah, and by one to two hours for Ranger Russet. This study confirmed monitoring for new and developing bruises can be utilized as an early indicator of damage incidence in samples from handling operations.</p></div>","PeriodicalId":7596,"journal":{"name":"American Journal of Potato Research","volume":"103 3","pages":"290 - 308"},"PeriodicalIF":1.8,"publicationDate":"2025-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12230-025-10027-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148237110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Creation of Clones with Multiple Multiplexed Resistance Genes Useful in Potato Breeding","authors":"Kazuyoshi Hosaka,&nbsp;Rena Sanetomo,&nbsp;Kenji Katayama,&nbsp;Seiji Tamiya,&nbsp;Kenji Asano,&nbsp;Etsuo Shimosaka,&nbsp;Kotaro Akai,&nbsp;Hitomi Nakajima,&nbsp;Satoshi Okamoto","doi":"10.1007/s12230-025-10032-8","DOIUrl":"10.1007/s12230-025-10032-8","url":null,"abstract":"<div><p>To broaden genetic diversity in the breeders’ gene pool, we previously selected 95 long-day-adapted hybrid clones from Andean cultivated potatoes. In this study, these clones were crossed to combine resistance genes for golden cyst nematode (<i>H1</i>), <i>Potato virus Y</i> (<i>Ry</i>_<i>chc</i>), and <i>Potato virus X</i> (<i>Rx1</i>) into individual clones using marker assays through multiplex PCR. Additionally, effective late blight resistance genes, <i>Rpi-blb3</i> and/or <i>R2</i>, were incorporated. After field evaluation, the selected clones were crossed and marker-assayed with real-time PCR to increase the copy number (dosage) of the resistance genes. After two rounds of increasing the copy number, 16 clones exhibited an average copy number of 3.00 for <i>H1</i>, 2.56 for <i>Ry</i>_<i>chc</i>, and 2.81 for <i>Rx1</i>, while 18 clones showed an average copy number of 2.56 for <i>H1</i>, 1.94 for <i>Ry</i>_<i>chc</i>, and 2.06 for <i>Rx1,</i> along with 0.83 for <i>Rpi-blb3</i> and 0.61 for <i>R2</i>. These 34 clones possess multiple, multiplexed resistance genes, which are valuable for breeding programs.</p></div>","PeriodicalId":7596,"journal":{"name":"American Journal of Potato Research","volume":"103 2","pages":"188 - 199"},"PeriodicalIF":1.8,"publicationDate":"2025-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12230-025-10032-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147735064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of Tissue Culture Media for Microtuber Development Incorporating Sugars and Zinc Nanoparticles","authors":"Muzammil Anwar Khan,&nbsp;Naima Huma Naveed,&nbsp;Aamir Ali,&nbsp;Muhammad Adeel,&nbsp;Aima Iram Batool,&nbsp;Tahir Shahzad,&nbsp;Muhammad Rizwan Khan,&nbsp;Salman Majeed","doi":"10.1007/s12230-025-10028-4","DOIUrl":"10.1007/s12230-025-10028-4","url":null,"abstract":"<div><p>This study deals with the long term problem of seed tuber scarcity to plant potato. The given work puts an emphasis on the effects of sucrose, cytokinins and zinc nanoparticles (ZnNPs) on potato microtuberization. The optimum level was screened by using different levels (3%, 5%, 6%, 7%, and 8%) of sucrose in Murashige and Skoog media. 6% sucrose emerged as the most effective concentration and was used in combination with varying concentrations of cytokinins (6-Benzylaminopurine and Kinetin). Cytokinins did not exhibit any significant improvement in microtuberization. The study focused on investigating the effect of ZnNPs in combination with different concentrations of sucrose on the microtuberization. It was found out that 40 mg kg^− 1 ZnNPs and 6% sucrose resulted in the maximum number of microtubers, that is 5.33 ± 0.67 microtubers within 14 days having average tuber weight of 0.70 g. Biochemical analysis was carried out to check the influence of ZnNPs on various parameters. The recorded increment in total soluble protein contents is directly correlated with microtuber formation and growth. Similarly, at the time of microtuber induction total soluble sugar contents, particularly non-reducing sugars were higher than reducing sugar. Specific activity of nitrate reductase and enzymes such as phenylalanine ammonia-lyase, peroxidase and polyphenol oxidase were also enhanced in response to microtuberization. This analysis provided insights into the biochemical changes occurring after application of ZnNPs.</p></div>","PeriodicalId":7596,"journal":{"name":"American Journal of Potato Research","volume":"103 2","pages":"148 - 163"},"PeriodicalIF":1.8,"publicationDate":"2025-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147735061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}