{"title":"Evaluation of brucellin skin test for bovine brucellosis.","authors":"C C Chukwu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The diagnostic efficiency of the Brucellin skin test for bovine brucellosis was evaluated using cattle of known history. The test was negative in six out of 14 heifers (42.9%) infected with virulent Brucella abortus (Br. abortus) strain 544. In four cattle vaccinated with a reduced dose of Br. abortus strain 19 vaccine (5 X 10(9) live organisms) the skin test became positive in all the animals but two weeks after immunization. However, all the vaccinates became negative 14 weeks after vaccination, whereas nine out of 14 heifers (64.3%) vaccinated with killed Br. abortus 45/20 adjuvant vaccine (Duphavac vaccine) were still positive 18 months post immunization. Four control cattle were persistently negative. It was considered that the procedure would be most useful for testing non-exposed cattle and should be used as a screen test. It is not useful as a diagnostic test for cattle immunized with Duphavac vaccine. A recommendation is made for interpreting the Brucellin skin test for the diagnosis of bovine brucellosis in non-exposed herds.</p>","PeriodicalId":75942,"journal":{"name":"International journal of zoonoses","volume":"12 1","pages":"6-13"},"PeriodicalIF":0.0,"publicationDate":"1985-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15048004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Direct immunoperoxidase test in the diagnosis of rabies--an alternative to fluorescent antibody test.","authors":"S Kotwal, K G Narayan","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Direct immunoperoxidase test was run parallel to fluorescent antibody test using known rabies-positive and negative materials. Both the tests had 100.0% sensitivity and specificity when mice brain infected with CVS strain of Rabies virus was used. With the field samples and the mice brains infected with these, the sensitivity of direct immunoperoxidase test and also of fluorescent antibody test dropped a little, giving an overall sensitivity of 95.3 and 99.0 percent respectively. The standardization of immunoperoxidase test is described and the results discussed.</p>","PeriodicalId":75942,"journal":{"name":"International journal of zoonoses","volume":"12 1","pages":"80-5"},"PeriodicalIF":0.0,"publicationDate":"1985-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15016904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Shedding of Coxiella burnetii in milk by Nigerian dairy and dual purposes cows.","authors":"A A Adesiyun, A G Jagun, J K Kwaga, L B Tekdek","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Fresh milk from dairy cows kept at semi-intensive husbandry in governmental and institutional farms and dual purpose Fulani nomadic husbandry around Zaria, Nigeria were screened for Coxiella burnetii using the mouse inoculation test. Of the 20 herds from both management systems tested, 16(80.0%) contained dairy cows shedding C. burnetii in their milk. Of a total of 169 cows tested, 41(24.3%) were shedders. Eighteen (22.0%) of 82 cows kept under semi-intensive and 23(26.4%) of 87 cows kept under Fulani nomadic systems were shedding C. burnetii. The difference in frequency of shedding C. burnetii between both system was not statistically significant (P greater than 0.05, X2). Of the 88 dairy cows serologically tested for C. burnetii agglutinins by the capillary agglutination test (C.A.T), 48(54.5%) were sero-positive and 40(45.5%) were seronegative. Among Q-fever sero-positive cows, 11(22.9%) were shedders and 37(77.1%) were not shedding C. burnetii in their milk. Of the sero-negative cows, 6(15.0%) and 34(85.0%) were shedders and non-shedders, respectively. With milk from Fulani nomadic cows predominantly taken raw or made into unheated fermented milk products, the health risk to the consumers of such products cannot be over-emphasized.</p>","PeriodicalId":75942,"journal":{"name":"International journal of zoonoses","volume":"12 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"1985-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15166843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparative study of four saprophytic leptospira strains as screening antigens in the serodiagnosis of leptospirosis in water buffaloes (Bubalus bubalis).","authors":"R J Girio, R M Yanaguita, L A Mathias","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The profitability of four saprophytic leptospira strains (Buenos Aires, Patoc 1, Rufino and São Paulo) as polyvalent antigen in the serodiagnosis of leptospirosis in water buffaloes was studied by the microscopic agglutination test. From 104 examined sera tested against 16 pathogenic leptospira serotypes, 52 were positives and 52 were negatives. The results led to the conclusion that from four studied strains, Buenos Aires strain showed the best results when utilized in screening tests for the serodiagnosis of leptospirosis in water buffaloes. The other three strains showed a good specificity but their sensibilities were poor and therefore should not be recommended for diagnosing water buffalo leptospirosis.</p>","PeriodicalId":75942,"journal":{"name":"International journal of zoonoses","volume":"12 1","pages":"61-6"},"PeriodicalIF":0.0,"publicationDate":"1985-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15166848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Serological investigation on cattle vaccinated with a killed Brucella abortus strain 45/20 adjuvant vaccine.","authors":"C C Chukwu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Serum antibody reactions were monitored for a period of 18 months in 18 heifers which had been vaccinated with Brucella abortus Killed 45/20 adjuvant vaccine, using four standard serological procedures: the Rose Bengal, Serum agglutination, Complement fixation and Coombs antibovine globulin tests. The Rose Bengal and Serum agglutination tests were persistently negative during the experiment. Titres to the complement fixation test rose sharply for only four weeks after the second dose of the vaccine. Thereafter the titres fluctuated to positive levels at unpredictable intervals. One out of 14 heifers tested 12 months after vaccination was strongly positive to the complement fixation test but all the animals were absolutely negative at the end of the experiment. The Coombs test became strongly positive after the second dose of the vaccine for up to 34 weeks after primary vaccination. The animals were negative to the test at the end of the experimental period but some still retained suspicious titres. It was concluded that the Brucella abortus killed 45/20 adjuvant vaccine did not interfer stall with the Rose Bengal and Serum agglutination tests, that the fluctuations of the Complement fixation titres to positive levels at unpredictable intervals were undesirable and that the Coombs test showed little promise as an assay which would be used to differentiate antibody titres due to Brucella abortus Killed 45/20 adjuvant vaccine from those of natural infection. It would appear that the Complement fixation test and the Coombs antibovine globulin test could be safely used 12 and 18 months respectively, after immunization of cattle with the vaccine.</p>","PeriodicalId":75942,"journal":{"name":"International journal of zoonoses","volume":"12 1","pages":"14-21"},"PeriodicalIF":0.0,"publicationDate":"1985-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15048003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Gastrointestinal parasites of cattle in Nigeria. I. An abattoir survey on the prevalence of trematode parasites.","authors":"A F Ogunrinade","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75942,"journal":{"name":"International journal of zoonoses","volume":"12 1","pages":"35-8"},"PeriodicalIF":0.0,"publicationDate":"1985-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14992329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The numbers and varieties of bacteria carried by filth flies in sanitary and unsanitary city area.","authors":"O Adeyemi, O O Dipeolu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Filth flies caught with nets in various localities of varying sanitary conditions in Ibadan city were predominantly Musca domestica and a few Fannia cannicularis. Seven genera of bacteria, some of which were pathogenic to humans, were isolated from the legs, wings, mouthparts and midguts of the flies. Flies were very abundant in areas where unsanitary conditions prevailed and scarce where sanitary conditions were enforced. The total number of bacteria isolated from flies caught in the market places was higher than those isolated from flies caught in any other locality; low numbers of bacteria were isolated from flies caught in areas where hygienic conditions prevailed. Bacillus spp. were the most numerous of the bacteria isolated. The greatest numbers of bacteria were found on the legs. From the house-flies caught on dairy animals were isolated a high number of Escherichia coli. The public health significance of these findings is discussed.</p>","PeriodicalId":75942,"journal":{"name":"International journal of zoonoses","volume":"11 2","pages":"195-203"},"PeriodicalIF":0.0,"publicationDate":"1984-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17592191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Epidemiology of bovine brucellosis in Mexicali Valley, Mexico: use of path analysis to refine the existing control program.","authors":"M D Salman, M E Meyer, G Peralta","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>This paper describes how path analysis, a quantitative analytical technique, was used to evaluate details on the epidemiology of bovine brucellosis in a discrete geographic area of Baja California Norte, Mexico. The steps involved in the analysis are discussed and details are presented for refining and improving the bovine brucellosis control program in this geographic area.</p>","PeriodicalId":75942,"journal":{"name":"International journal of zoonoses","volume":"11 2","pages":"216-22"},"PeriodicalIF":0.0,"publicationDate":"1984-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17592193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Identification of species and biotypes of the brucella genus in apparently healthy and aborted ewes and goats in Egypt.","authors":"M Abdel-Ghani, S Abdel-Hamed, S M Nada, K Osman","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Brucella micro-organisms was absent in the vaginal swabs collected from apparently healthy ewes. Such incidence among the genital tract of aborted ewes was 4.84%. Among goats, brucella species represented 7.04% in aborted goats, while it was recovered in lower percentage (0.92%) from the vaginal swabs of apparently healthy goats. Br. melitensis biotype \"3\" was the sole species recovered from aborted ewes, while biotypes \"3\" and \"2\" could be obtained from clinically healthy and aborted foeti of goats. The predilection seats of brucella in the genital tracts of aborted animals and their foeti were discussed in details.</p>","PeriodicalId":75942,"journal":{"name":"International journal of zoonoses","volume":"11 2","pages":"139-44"},"PeriodicalIF":0.0,"publicationDate":"1984-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17591151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C E Larsson, C A Santa Rosa, M K Hagiwara, G V Paim, J L Guerra
{"title":"Prevalence of feline leptospirosis: serologic survey and attempts of isolation and demonstration of the agent.","authors":"C E Larsson, C A Santa Rosa, M K Hagiwara, G V Paim, J L Guerra","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The occurrence of leptospiral infection in cats was determined through the detection of specific antibodies based on the results of microscopic agglutination test and the attempts of isolation and histological demonstration of leptospires from the kidneys of these animals. Of 172 serum samples examined by microscopic agglutination test, 22 (12.8%) were positive with titers greater than or equal to 100. The most frequent serovar was pomona. In relation to the sex, significant differences were not seen; however the age distribution showed that feline leptospirosis is more frequent in adult cats. The attempts for isolation and demonstration of L. interrogans from renal parenchyma by culture or Warthin Starry technics were unsuccessful.</p>","PeriodicalId":75942,"journal":{"name":"International journal of zoonoses","volume":"11 2","pages":"161-9"},"PeriodicalIF":0.0,"publicationDate":"1984-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17592187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}