Direct immunoperoxidase test in the diagnosis of rabies--an alternative to fluorescent antibody test.

Abstract

Direct immunoperoxidase test was run parallel to fluorescent antibody test using known rabies-positive and negative materials. Both the tests had 100.0% sensitivity and specificity when mice brain infected with CVS strain of Rabies virus was used. With the field samples and the mice brains infected with these, the sensitivity of direct immunoperoxidase test and also of fluorescent antibody test dropped a little, giving an overall sensitivity of 95.3 and 99.0 percent respectively. The standardization of immunoperoxidase test is described and the results discussed.