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Reversible norepinephrine binding to rabbit myometrium: relationship to sites of known biological significance. 可逆去甲肾上腺素与兔肌层的结合:与已知生物学意义位点的关系。
Gynecologic investigation Pub Date : 1976-01-01 DOI: 10.1159/000301390
J M Roberts, D W Keller, A Goldfien
{"title":"Reversible norepinephrine binding to rabbit myometrium: relationship to sites of known biological significance.","authors":"J M Roberts,&nbsp;D W Keller,&nbsp;A Goldfien","doi":"10.1159/000301390","DOIUrl":"https://doi.org/10.1159/000301390","url":null,"abstract":"<p><p>Catecholamines bind specifically to several cell types and subcellular fractions. This binding is not consistent with binding to the adrenergic receptor. Catecholamines would be expected to bind to sites other than the adrenergic receptor (uptake 1, uptake 2, nerve vesicles, catecholamine-O-methyl transferase and serum albumin). We characterized the binding of 3H-norepinephrine to microsomes prepared from rabbit myometrium. In the presence of antioxidant binding was reversible and 3H-norepinephrine could be recovered bound to microsomes or free in incubation media. The binding was not characteristic of binding to any one known biological site. It may represent binding to a mixture of these sites or may be fortuitous binding to sites of unknown biological significance.</p>","PeriodicalId":75889,"journal":{"name":"Gynecologic investigation","volume":"7 5","pages":"293-305"},"PeriodicalIF":0.0,"publicationDate":"1976-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000301390","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11235500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
An analysis of the mechanisms of egg transport in the ampulla of the rabbit oviduct. 家兔输卵管壶腹内卵子转运机制的分析。
Gynecologic investigation Pub Date : 1976-01-01 DOI: 10.1159/000301391
S A Halbert, P Y Tam, R J Adams, R J Blandau
{"title":"An analysis of the mechanisms of egg transport in the ampulla of the rabbit oviduct.","authors":"S A Halbert,&nbsp;P Y Tam,&nbsp;R J Adams,&nbsp;R J Blandau","doi":"10.1159/000301391","DOIUrl":"https://doi.org/10.1159/000301391","url":null,"abstract":"<p><p>Ampullary transport of supravitally stained cumulus egg masses was studied in intact oviducts of anesthetized rabbits whose abdomens had been opened for observation. Following observations of normal transport, muscular activity of the ampulla was inhibited pharmacologically with Acepromazine, a preanesthetic tranquilizer. With muscle contractions blocked, egg transport continued but in a dramatically altered fashion; in the final two thirds of the ampulla the motion changed from rapid to-and-fro movements to a slow uniform prouterine movement which was attributed to ciliary activity. However, the net velocity of transport did not change when the smooth muscle was inhibited indicating that muscle contractions are at least unnecessary and perhaps ineffective for ampullary egg transport in the rabbit.</p>","PeriodicalId":75889,"journal":{"name":"Gynecologic investigation","volume":"7 5","pages":"306-20"},"PeriodicalIF":0.0,"publicationDate":"1976-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000301391","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12183742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 14
Intrauterine fetal sex determonation by radioimmunoassay of amniotic fluid testosterone. 羊水睾酮放射免疫法测定宫内胎儿性别。
Gynecologic investigation Pub Date : 1975-11-01 DOI: 10.1097/00006254-197511000-00005
H. Giles, C. Lox, M. W. Heine, C. Christian
{"title":"Intrauterine fetal sex determonation by radioimmunoassay of amniotic fluid testosterone.","authors":"H. Giles, C. Lox, M. W. Heine, C. Christian","doi":"10.1097/00006254-197511000-00005","DOIUrl":"https://doi.org/10.1097/00006254-197511000-00005","url":null,"abstract":"","PeriodicalId":75889,"journal":{"name":"Gynecologic investigation","volume":"5 5-6 1","pages":"317-23"},"PeriodicalIF":0.0,"publicationDate":"1975-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1097/00006254-197511000-00005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"61910500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Egg transport in the fallopian tube. 卵子在输卵管中的运输。
Gynecologic investigation Pub Date : 1975-01-01 DOI: 10.1159/000301518
H B Croxatto, M E Ortiz
{"title":"Egg transport in the fallopian tube.","authors":"H B Croxatto,&nbsp;M E Ortiz","doi":"10.1159/000301518","DOIUrl":"https://doi.org/10.1159/000301518","url":null,"abstract":"<p><p>The transport of eggs from the site of ovulation to the site of implantation is a fundamental step of the reproductive process in the female. The fallopian tube effects the major part of this function and times the passage of eggs into the endometrial environment. As a result of different combinations of speed of progression and retention periods through the various regions of the oviduct, the pattern of transport differs from one species to another. The role of muscular contraction, ciliary movement and flow of secretions in the mechanisms of progression and retention are still poorly understood. Estrogens and progestins have a pronounced influence upon egg transport, but the responses to exogenous hormones are quite variable and depend mainly upon species, dose and time of administration. Species differences prevent from broader generalizations at this time and indicate the need for further comparative studies.</p>","PeriodicalId":75889,"journal":{"name":"Gynecologic investigation","volume":"6 3-4","pages":"215-25"},"PeriodicalIF":0.0,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000301518","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11997365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 56
Capacitation, ovum maturation, fertilization and preimplantation development in the oviduct. 输卵管内的获能、卵子成熟、受精和着床前发育。
Gynecologic investigation Pub Date : 1975-01-01 DOI: 10.1159/000301519
L Mastroianni, J Komins
{"title":"Capacitation, ovum maturation, fertilization and preimplantation development in the oviduct.","authors":"L Mastroianni,&nbsp;J Komins","doi":"10.1159/000301519","DOIUrl":"https://doi.org/10.1159/000301519","url":null,"abstract":"<p><p>The oviduct provides a temporary environment for spermatozoa, the ovum, and the embryo during the first 3 days of development. The milieu in the tubal lumen is ideal for fertilization and then events which precede it. Conditions which influence spermatozoa and the ovum are assessed, and the factors responsible for fertilization and normal development thereafter analyzed. Constituents of tubal fluid which have functional significance include bicarbonate, lactate, and pyruvate as well as ions in a suitably balanced ratio following ovulations. The functional significance of cyclic changes in protein patterns remains to be explored. Further analysis of tubal fluid content and its regulation, and additional exploration of conditions which support fertilization and early development are needed to provide a more complete understanding of the role of the oviduct in reproduction.</p>","PeriodicalId":75889,"journal":{"name":"Gynecologic investigation","volume":"6 3-4","pages":"226-33"},"PeriodicalIF":0.0,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000301519","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11997366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 13
Contractility of the fallopian tube. 输卵管的收缩。
Gynecologic investigation Pub Date : 1975-01-01 DOI: 10.1159/000301514
E M Coutinho, H Maia, C E Mattos
{"title":"Contractility of the fallopian tube.","authors":"E M Coutinho,&nbsp;H Maia,&nbsp;C E Mattos","doi":"10.1159/000301514","DOIUrl":"https://doi.org/10.1159/000301514","url":null,"abstract":"<p><p>Recordings of intratubal pressure in women reveal a complex pattern of tubal contractions. Overall activity of the fallopian tube is increased during ovulation. During the luteal phase of the menstrual cycle, activity is depressed but never suppressed. The fallopian tube remains active during gestation in contrast with the uterus which is quiescent. The early puerperal tube displays a pattern of activity similar to that recorded during the late luteal phase of the menstrual cycle. Activation occurs during suckling and in response to exogenous oxytocin, prostaglandins, nor-adrenergic compounds, and ergot derivatives. It is concluded that contractility of the fallopian tube is less influenced by ovarian steroids than that of the uterus.</p>","PeriodicalId":75889,"journal":{"name":"Gynecologic investigation","volume":"6 3-4","pages":"146-61"},"PeriodicalIF":0.0,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000301514","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11388070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 18
Early stimulation of human chorionic gonadotropin secretion by dibutyryl cyclic AMP and theophylline in human malignant trophoblast cells in vitro: inhibition by actinomycin D, alpha-amanitin, and cordycepin. 二丁基环AMP和茶碱对体外人恶性滋养细胞绒毛膜促性腺激素分泌的早期刺激:放线菌素D、α -amanitin和虫草素的抑制作用
Gynecologic investigation Pub Date : 1975-01-01 DOI: 10.1159/000301538
R A Pattillo, O Hussa
{"title":"Early stimulation of human chorionic gonadotropin secretion by dibutyryl cyclic AMP and theophylline in human malignant trophoblast cells in vitro: inhibition by actinomycin D, alpha-amanitin, and cordycepin.","authors":"R A Pattillo,&nbsp;O Hussa","doi":"10.1159/000301538","DOIUrl":"https://doi.org/10.1159/000301538","url":null,"abstract":"<p><p>Human malignant trophoblast cells (BeWo line) in culture were employed to investigate the early stimulation of human chorionic gonadotropin (hCG) secretion by 1 mM dibutyryl cyclic AMP and 1 mM theophylline (dbT). The earliest increase in secreted immunoreactive hCG occurred at 3 1/2 h following addition of dbT, and was preceded by an increase in intracellular hCG. These results suggested that dbT stimulated hCG synthesis, rather than release. Addition of either 0.062 mug/ml actinomycin D or 100 mug/ml cordycepin along with dbT, or 3 mug/ml alpha-amanitin 9 h prior to addition of dbT, prevented the increase in hCG secretion at 3 1/2-8 h. It was concluded that synthesis of RNA (possibly messenger RNA) is required for the early stimulation of hCG secretion by dbT.</p>","PeriodicalId":75889,"journal":{"name":"Gynecologic investigation","volume":"6 6","pages":"365-77"},"PeriodicalIF":0.0,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000301538","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11392271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 18
Antibacterial activity of human cervical mucus. 人宫颈黏液的抗菌活性。
Gynecologic investigation Pub Date : 1975-01-01 DOI: 10.1159/000301522
H Zuckerman, A Kahana, S Carmel
{"title":"Antibacterial activity of human cervical mucus.","authors":"H Zuckerman,&nbsp;A Kahana,&nbsp;S Carmel","doi":"10.1159/000301522","DOIUrl":"https://doi.org/10.1159/000301522","url":null,"abstract":"<p><p>The antimicrobial property of human uterine cervical mucus was tested in three groups of women. Healthy women, using no contraception, women using an intrauterine device and women receiving hormonal treatment for contraception. Cervical mucus was taken on the 10th, 14th, 18th and 22nd day of the menstrual cycle. Cervical mucus had a strong inhibitory effect on the growth of Micrococcus lysodeicticus in all three groups. The strength of the inhibitory effect on the other microorganisms were in the following order: Staphylococcus albus, Staphylococcus aureus, Proteus mirabilis, Escherichia coli, Candida albicans, Streptococcus haemolyticus, Streptococcus faecalis. Use of an intrauterine device did not affect the antimicrobial effect of cervical mucus. The use of hormonal contraceptive canceled the antimicrobial effect on the series of microorganisms, with the exception of M. lysodeicticus. The maximum inhibitory effect occurred on the 14th day and declined toward the end of the menstrual cycle.</p>","PeriodicalId":75889,"journal":{"name":"Gynecologic investigation","volume":"6 5","pages":"265-71"},"PeriodicalIF":0.0,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000301522","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11997369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 21
Experimental production of aneuploidy in mouse oocytes. 小鼠卵母细胞非整倍体的实验产生。
Gynecologic investigation Pub Date : 1975-01-01 DOI: 10.1159/000301532
L E Karp, W D Smith
{"title":"Experimental production of aneuploidy in mouse oocytes.","authors":"L E Karp,&nbsp;W D Smith","doi":"10.1159/000301532","DOIUrl":"https://doi.org/10.1159/000301532","url":null,"abstract":"<p><p>The normal course of meiosis can be interrupted in cultured mouse oocytes by exposre to low temperature (23 degrees C). Re-transfer of the oocytes to a 37 degrees C environment will permit the meiotic process to resume and progress as usual to second metaphase. However, a significant number of oocytes treated in this manner prove to contain abnormal numbers of chromosomes.</p>","PeriodicalId":75889,"journal":{"name":"Gynecologic investigation","volume":"6 6","pages":"337-41"},"PeriodicalIF":0.0,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000301532","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12388737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 24
Localization of anti-HPL in fetal, placental, and maternal renal tissues. 抗hpl在胎儿、胎盘和母体肾组织中的定位。
Gynecologic investigation Pub Date : 1975-01-01 DOI: 10.1159/000301528
J P Gudson, M R Caudle, G A Herbst
{"title":"Localization of anti-HPL in fetal, placental, and maternal renal tissues.","authors":"J P Gudson,&nbsp;M R Caudle,&nbsp;G A Herbst","doi":"10.1159/000301528","DOIUrl":"https://doi.org/10.1159/000301528","url":null,"abstract":"<p><p>Anti-human placental lactogen (HPL) was given to pregnant rats. An analysis of various tissues including lung, muscle, placenta, kidney and fetal localizes the heterologous antibody at the placenta. It is presumed that whatever destructive effect it has takes place there.</p>","PeriodicalId":75889,"journal":{"name":"Gynecologic investigation","volume":"6 6","pages":"321-8"},"PeriodicalIF":0.0,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000301528","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12418109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
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