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Application on Timm sulphide silver method for electron microscope localization of lead ions in blood cells. 硫化银法在血细胞中铅离子电镜定位中的应用。
Folia haematologica (Leipzig, Germany : 1928) Pub Date : 1990-01-01
H Grzybek, W Sliwa-Tomczok, J Tomczok
{"title":"Application on Timm sulphide silver method for electron microscope localization of lead ions in blood cells.","authors":"H Grzybek,&nbsp;W Sliwa-Tomczok,&nbsp;J Tomczok","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The application of the Timm sulphide silver method for the demonstration of lead ions in peripheral blood cells is investigated, using male white adult Wistar rats treated with a single dose of lead acetate, range 150 mg/kg b.w. To improve the Timm reaction for electron microscopy, fixation of whole cells with glutaraldehyde fixative solution saturated with H2S, an agarose embedding and physical development of thick sections without prior cryostat sectioning are presented. At 6.0 hours after injection both erythrocytes as well as white cells reveal the positive Timm reaction. All types of blood cells contain numerous cytoplasmic precipitates illustrating the intracellular lead accumulation. It is shown that the invaginations of white cell nuclear membrane possess a storing function as areas of lead depots. As a rule, the neutrophils display a highest amount of cytoplasmic precipitates and exclusively a low amount of reaction products in basophils is observed. At 14 days after injection, precipitates are present only in erythrocytes and monocytes. A suggestion of a possible functional significance of changes in the Timm staining pattern in blood cell types is discussed in this paper.</p>","PeriodicalId":75853,"journal":{"name":"Folia haematologica (Leipzig, Germany : 1928)","volume":"117 2","pages":"277-82"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12862352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Index of phagocyte activation based on luminescence of dehydrogenase coenzymes. 基于脱氢酶辅酶发光的吞噬细胞活化指标。
Folia haematologica (Leipzig, Germany : 1928) Pub Date : 1990-01-01
A A Slavinsky
{"title":"Index of phagocyte activation based on luminescence of dehydrogenase coenzymes.","authors":"A A Slavinsky","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In the cytoplasm of activated neutrophils and monocytes of blood, as well as microphages and macrophages of festering wounds, the incubation with nitroblue tetrazolium results, in two types of autoluminescence. The blue luminescence as a result of reduced pyridine nucleotides (RP) characterizes the non-active state of phagocytes. The yellow-green luminescence due to oxygenated flavoproteins (OF) signifies the active state of phagocytes. The luminescence intensity ratio of OF to RP reflects the rate of energy output and is the energy index of phagocyte activation.</p>","PeriodicalId":75853,"journal":{"name":"Folia haematologica (Leipzig, Germany : 1928)","volume":"117 3","pages":"365-8"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12867495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Haematological complications in polycythaemia vera and thrombocythaemia patients treated with radiophosphorus (32P). 真性红细胞增多症和血小板增多症患者放射磷治疗的血液学并发症(32P)
Folia haematologica (Leipzig, Germany : 1928) Pub Date : 1990-01-01
M L Randi, F Fabris, L Varotto, C Rossi, C Macri, A Girolami
{"title":"Haematological complications in polycythaemia vera and thrombocythaemia patients treated with radiophosphorus (32P).","authors":"M L Randi,&nbsp;F Fabris,&nbsp;L Varotto,&nbsp;C Rossi,&nbsp;C Macri,&nbsp;A Girolami","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We have evaluated 230 patients with myeloproliferative disorders treated in the last 15 years with 32P. None of the patients affected by essential thrombocythaemia developed haematological complications. In the larger group of polycythaemia patients (214 subjects) only 38 patients (17 males and 21 females) developed complications. 60.5% of these subjects had a minor complications: 1.8% showed a thrombocytopenia lower than 100.10e9/lt, 2.3% anaemia with Hb lower than 10 g%, 2.6% leukopenia lower than 40.10e9/lt and 2.3% a pancytopenia. All these complications were transient and eventually treated with limited blood transfusions. We could not identify a correlation between the dose used and the development of such complications. We noted only that the occurrence of anaemia, given a similar dose, was more frequent in females. Only 7% of all patients presented a major complication after 32P administration. In this case too, there was no correlation with the dose administered. Myelofibrosis and chronic myeloid leukaemia resulted to be the more frequent complication (9 out of 15) but we could not clarify if they represented a natural evolution of polycythaemia vera or were due to the treatment with 32P. Acute leukaemia developed only in 5 patients and again we could not recognized a correlation with the dose administered. Moreover, the time from the diagnosis of polycythaemia vera the onset of acute leukaemia ranged widely. 32P has a definite effect on the prevention of thrombotic and haemorrhagic complications in polycythaemia patients since it prolongs their life but it also increases the incidence of acute leukaemia.</p>","PeriodicalId":75853,"journal":{"name":"Folia haematologica (Leipzig, Germany : 1928)","volume":"117 3","pages":"461-7"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12867925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Computer evaluation of reference limits. 参考限值的计算机评定。
Folia haematologica (Leipzig, Germany : 1928) Pub Date : 1990-01-01
J Berger
{"title":"Computer evaluation of reference limits.","authors":"J Berger","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Reference limits are necessary for medical laboratory to provide objective information on health in concrete characteristics. With respect to changeability of some factors influencing upon homeostasis, reference limits must be determined and redetermined in each clinical laboratory with the high degree of the internal quality control system. The presented program computing reference limits and providing expert consultations facilitates solution.</p>","PeriodicalId":75853,"journal":{"name":"Folia haematologica (Leipzig, Germany : 1928)","volume":"117 3","pages":"475-8"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12867927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Influence of the monocyte culture on thromboxane A2 level and platelet aggregation. 单核细胞培养对血栓素A2水平及血小板聚集的影响。
Folia haematologica (Leipzig, Germany : 1928) Pub Date : 1990-01-01
J Giedrojć, T Czaczkowska, M Bielawiec
{"title":"Influence of the monocyte culture on thromboxane A2 level and platelet aggregation.","authors":"J Giedrojć,&nbsp;T Czaczkowska,&nbsp;M Bielawiec","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The importance of circulating blood cells has been further emphasized as monocytes may also contribute to the pathogenesis of atherosclerosis. The pathological changes in atheroma may be partly mediated by metabolites of arachidonic acid. The aim of this study was determining thromboxane B2 concentrations in the supernatants of monocytes cultures and its influence on platelet aggregation. Human blood monocytes were recovered according to the procedure of Böyum and incubated with LPS. It was shown that monocytes produced thromboxane A2. The culture supernatant of monocytes from 36 h cultivation exerts an independent effect on platelet aggregation, which has been modified by inhibitors of aggregating factors. The results may indicate the influence of monocytes on AA metabolism in particular TXB2 production, platelet function and indirectly on the atherosclerotic process.</p>","PeriodicalId":75853,"journal":{"name":"Folia haematologica (Leipzig, Germany : 1928)","volume":"117 3","pages":"479-84"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12867928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Nebulized heparin and anabolic steroid in the prevention of postoperative deep vein thrombosis following elective abdominal surgery. 肝素和合成代谢类固醇雾化预防选择性腹部手术后深静脉血栓形成。
Folia haematologica (Leipzig, Germany : 1928) Pub Date : 1990-01-01
K Zawilska, A Tokarz, A Misiak, P Psuja, S Wisławski, P Szymczak, J Meissner, J Karoń, K Lewandowski, S Lopaciuk
{"title":"Nebulized heparin and anabolic steroid in the prevention of postoperative deep vein thrombosis following elective abdominal surgery.","authors":"K Zawilska,&nbsp;A Tokarz,&nbsp;A Misiak,&nbsp;P Psuja,&nbsp;S Wisławski,&nbsp;P Szymczak,&nbsp;J Meissner,&nbsp;J Karoń,&nbsp;K Lewandowski,&nbsp;S Lopaciuk","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>One hundred eighty three patients, all over 40 years old, who underwent major abdominal surgery, were randomized into 3 groups: Group I received a single dose of nebulized heparin (800 IU per kg b.w.) administered by inhalation one day prior to surgery. Group II besides the above, also received a single injection of 50 mg of long acting anabolic steroid (nandrolone phenylpropionate) intramuscularly. Group III received 5000 IU heparin subcutaneously on hr prior to surgery as well as every 12 h for the next 5 postoperative days. Postoperatively the patients were evaluated for deep vein thrombosis (DVT) using the 125-I-fibrinogen test. The occurrence of DVT was determined as: in Group I--16%, in Group II--7.9%, in Group III--7.8%. Haemorrhagic complications (clinically important) were observed in 7.8% of patients from Group III, but only in 1.7% of patients in Group I and 1.6% in Group II. For DVT prophylaxis following abdominal surgery a single application of nebulized heparin and long acting anabolic steroid is as effective as conventional low-dose subcutaneous heparin administration, but gives less haemorrhagic complications. This method is also more advantagenous in term of acceptance by the patients and represents considerable saving of nursing time.</p>","PeriodicalId":75853,"journal":{"name":"Folia haematologica (Leipzig, Germany : 1928)","volume":"117 5","pages":"699-707"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12874170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Iron resorption and the effects of iron supplementation in blood donors]. 献血者的铁吸收和补铁的影响。
Folia haematologica (Leipzig, Germany : 1928) Pub Date : 1990-01-01
L H Schmidt, W Gulich, M Schröder, V Drescher
{"title":"[Iron resorption and the effects of iron supplementation in blood donors].","authors":"L H Schmidt,&nbsp;W Gulich,&nbsp;M Schröder,&nbsp;V Drescher","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Topical values for some haematological factors such as Hb and Ery as well as transferrin and ferritin were determined in 7 female blood donors and 8 male blood donors during the years of their work as blood donors. Subsequently, an iron resorption test was carried out which unexpectedly resulted in low rates of resorption ranging from 5.6% in men to 3.7% in women. After supplementing with vitaferro for 3 months the ferritin values which initially lay around the lower limiting value of 20 or 10 mg/l in men or women had doubled.</p>","PeriodicalId":75853,"journal":{"name":"Folia haematologica (Leipzig, Germany : 1928)","volume":"117 6","pages":"823-8"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12878091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Haemocompatibility of extracorporeal circulation technique with autooxygenation: influence on platelet function and homologous blood requirement. 自体氧合体外循环技术的血液相容性:对血小板功能和同源血需氧量的影响。
Folia haematologica (Leipzig, Germany : 1928) Pub Date : 1990-01-01
J Wojnar, A Bochenek, A M Wnuk-Wojnar, Z Religa, J Hołowiecki
{"title":"Haemocompatibility of extracorporeal circulation technique with autooxygenation: influence on platelet function and homologous blood requirement.","authors":"J Wojnar,&nbsp;A Bochenek,&nbsp;A M Wnuk-Wojnar,&nbsp;Z Religa,&nbsp;J Hołowiecki","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Forty male patients: group A-autooxygenation and group B-bubble oxygenator used in extracorporeal circulation (ECC) were studied to evaluate the haemocompatibility of 2 types of ECC. The Plt count dropped significantly in group B patients: -73% of initial value vs only -27% in group A, (p less than 0.001). In both groups a rise in BTG was shown, but higher in group B, p less than 0.001. At the end of CPB aggregation decreased only slightly in group A after epinephrine and 4-ADP, and decreased hardly in group B with the significant difference between two groups (p less than 0.02 and p less than 0.001, respectively). In group A the mean blood loss was 278 +/- 49 ml/m2 and 483 +/- 67 ml/m2 in group B, p less than 0.001. The mean blood transfusion in group A and B was 198 +/- 82 ml/m2 and 427 +/- 85 ml/m2, respectively (p less than 0.001). We are positive that the elimination of artificial oxygenator from the ECC diminished markedly the decline in Plt count and Plt activation during CPB.</p>","PeriodicalId":75853,"journal":{"name":"Folia haematologica (Leipzig, Germany : 1928)","volume":"117 6","pages":"875-85"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12878097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Inhibitors to factor IX contain all IgG subclasses except IgG3. 因子IX抑制剂包含除IgG3外的所有IgG亚类。
Folia haematologica (Leipzig, Germany : 1928) Pub Date : 1990-01-01
K H Orstavik
{"title":"Inhibitors to factor IX contain all IgG subclasses except IgG3.","authors":"K H Orstavik","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Five per cent of patients with haemophilia B develop inhibitors to factor IX. It is of interest to know the immunoglobulin subclass of these IgG antibodies. We have developed a sensitive method for the characterization of the subclass nature of inhibitors to factor IX. The technique is a crossed immunoelectrophoresis for the isolation of factor IX-inhibitor complexes followed by an enzyme-linked immunoassay using monoclonal antibodies to IgG subclasses for the subclass identification. We studied seven inhibitors with both low and high titres. One patient was studied at a very early stage of inhibitor development. All inhibitors gave a strong reaction with antibody to IgG4. Depending on the titre of the inhibitor, a reaction was also found with antibodies to IgG1 and IgG2. No inhibitor contained any detectable IgG3. IgG4 does not bind complement and it is therefore of importance that IgG4 is the main subclass both in high-titred and in low-titred inhibitors. The inhibitors are polyclonal antibodies, also at an early stage of inhibitor development.</p>","PeriodicalId":75853,"journal":{"name":"Folia haematologica (Leipzig, Germany : 1928)","volume":"117 4","pages":"545-8"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12878788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Improving the quality of washed and buffy coat-free erythrocyte concentrates]. [提高水洗和淡黄色无被毛红细胞浓缩物的质量]。
Folia haematologica (Leipzig, Germany : 1928) Pub Date : 1990-01-01
M Kaiser, M Scholz, D Strauss
{"title":"[Improving the quality of washed and buffy coat-free erythrocyte concentrates].","authors":"M Kaiser,&nbsp;M Scholz,&nbsp;D Strauss","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Washing buffy-coat free erythrocyte concentrates three times in bottles used for blood storage will diminish their leukocyte content to 0.22 +/- 0.11 x 10(9) per TE (= 9% of the initial value in whole blood, and the thrombocyte content to 0.3 +/- 0.5 x 10(9) per day (= 2% of the initial value in whole blood). Even 50% of leukocytes (mainly lymphocytes) and 80% of thrombocytes are eliminated simply by buffy coat separation. 30% of erythrocytes are lost by the washing process. Due to increasing haemolysis (0.22%) a subsequent storage of 24 hours should not be exceeded for washed erythrocyte concentrates. Further quality parameters, such as morphological index, pH, ATP, 2,3-P2G and K+ and Na+, were investigated. As far as selected quality parameters are concerned, washing erythrocyte concentrates three times in bottles for blood storage may be compared with washing them once in blood bags. The present findings confirm the conclusion that the washing of erythrocyte concentrates with a solution of sodium chloride in order to eliminate leukocytes may for the most part exclude non-haemolytic febrile transfusion reactions, but not immunization. More effective procedures of eliminating leukocytes, such as filtration, TTK or even glycerin, treatment of erythrocyte concentrates without cryoconservation, are indispensable.</p>","PeriodicalId":75853,"journal":{"name":"Folia haematologica (Leipzig, Germany : 1928)","volume":"117 1","pages":"79-88"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12859768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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