CRC critical reviews in biochemistry最新文献_第7页

Synthesis and properties of N-, O-, and S-phospho derivatives of amino acids, peptides, and proteins. 氨基酸、多肽和蛋白质的N-、O-和s -磷酸衍生物的合成和性质。

CRC critical reviews in biochemistry Pub Date : 1984-01-01 DOI: 10.3109/10409238409102806

A W Frank

引用次数: 26

Thermodynamics of hapten-antibody interactions. 半抗原抗体相互作用的热力学。

CRC critical reviews in biochemistry Pub Date : 1984-01-01 DOI: 10.3109/10409238409102301

T K Mukkur

引用次数: 0

Dye-ligand affinity chromatography: the interaction of Cibacron Blue F3GA with proteins and enzymes. 染料配体亲和层析:Cibacron蓝F3GA与蛋白质和酶的相互作用。

CRC critical reviews in biochemistry Pub Date : 1984-01-01 DOI: 10.3109/10409238409102302

S Subramanian

{"title":"Dye-ligand affinity chromatography: the interaction of Cibacron Blue F3GA with proteins and enzymes.","authors":"S Subramanian","doi":"10.3109/10409238409102302","DOIUrl":"https://doi.org/10.3109/10409238409102302","url":null,"abstract":"The dye Cibacron Blue F3GA has a high affinity for many proteins and enzymes. It has therefore been attached to various solid supports such as Sephadex, Sepharose, polyacrylamide, and the like. In the immobilized form the dye has rapidly been exploited as an affinity chromatographic medium to separate and purify a variety of proteins including dehydrogenases, kinases, serum albumin, interferons, several plasma proteins, and a host of other proteins. Such a diversity shown by the blue dye in binding several unrelated classes of proteins has generated considerable work in terms of studies of the chromophore itself and also the immobilized ligand. As a prelude to realizing the full potential of the immobilized Cibacron Blue F3GA, an understanding of the basic interactions of the dye with its surroundings must be gained. It has been recognized that the dye is capable of hydrophobic and/or electrostatic interactions at the instance of the ambient conditions. The study of interactions of the dye with salts, solvents, and other small molecules indicates the nature of the interactions of the dye with different kinds of groups at the interacting sites of proteins. The review will cover such interactions of the dye with the proteins, the interactions of the proteins with the immobilized ligand, and the media used to elute the bound protein in several cases, and thus consolidate the available information on such studies into a cogent and comprehensive explanation.","PeriodicalId":75744,"journal":{"name":"CRC critical reviews in biochemistry","volume":"16 2","pages":"169-205"},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10409238409102302","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17267883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 121

Introduction of purified genes into mammalian cells. 将纯化基因导入哺乳动物细胞。

CRC critical reviews in biochemistry Pub Date : 1984-01-01 DOI: 10.3109/10409238409108719

R Kucherlapati, A I Skoultchi

{"title":"Introduction of purified genes into mammalian cells.","authors":"R Kucherlapati, A I Skoultchi","doi":"10.3109/10409238409108719","DOIUrl":"https://doi.org/10.3109/10409238409108719","url":null,"abstract":"There are a number of methods to introduce genes into mammalian cells. These include cell hybridization, chromosome-mediated and DNA-mediated gene transfer. DNA-mediated transfer can be achieved by direct microinjection methods or by indirect methods. The DNA enters the nucleus and is expressed in a high proportion of cells transiently. The DNA then becomes integrated into host cell DNA at random sites resulting in more stably expressing transformants. A number of genes for which selection systems exist can be introduced into mammalian cells. Nonselectable genes can also be introduced into cells by either ligating them to a selectable gene or by mixing them with carrier DNA and a selectable gene. If an amplifiable gene sequence is introduced into cells, it and other genes in its proximity can be coamplified. Amplification of the genes can also be achieved by the use of appropriate viral vectors and recipient cells. The foreign genes are expressed in the recipient cells if they contain the appropriate recognition signals for initiation and termination of transcription. Transfection systems are thus permitting identification of DNA sequences which have a regulatory role in gene expression. The identification of transcriptional signal sequences has formed the basis for construction of appropriate molecules which would permit expression of genes which cannot normally be expressed in mammalian cells (e.g., bacterial genes). The foreign genes are not only expressed in the recipient cells but they can also be subject to regulation in the appropriate environment. This observation is paving the way for identification of regulatory sequences. The foreign DNA sequences integrated into the host genome can be recovered by a variety of methods. Such methods permit isolation of genes which code for a selectable gene product.","PeriodicalId":75744,"journal":{"name":"CRC critical reviews in biochemistry","volume":"16 4","pages":"349-79"},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10409238409108719","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17161311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 57

Processing of tRNA in prokaryotes and eukaryotes. tRNA在原核生物和真核生物中的加工。

CRC critical reviews in biochemistry Pub Date : 1984-01-01 DOI: 10.3109/10409238409110269

M P Deutscher

引用次数: 73

The synthesis and biology of fluorinated prostacyclins. 氟化前列环素的合成及生物学研究。

CRC critical reviews in biochemistry Pub Date : 1984-01-01 DOI: 10.3109/10409238409102802

W. E. Barnette

引用次数: 27

Structural and functional aspects of domain motions in proteins. 蛋白质结构域运动的结构和功能方面。

CRC critical reviews in biochemistry Pub Date : 1984-01-01 DOI: 10.3109/10409238409117796

W S Bennett, R Huber

引用次数: 219

The proton inventory technique. 质子库存技术。

CRC critical reviews in biochemistry Pub Date : 1984-01-01 DOI: 10.3109/10409238409110268

K S Venkatasubban, R L Schowen

引用次数: 152

Sucrase-isomaltase: a stalked intrinsic protein of the brush border membrane. 蔗糖酶-异麦芽糖酶:一种毛囊边缘膜的内在蛋白。

CRC critical reviews in biochemistry Pub Date : 1983-01-01 DOI: 10.3109/10409238309102798

H Hauser, G Semenza

引用次数: 22

Conformation and activity of beta-lactam antibiotics. -内酰胺类抗生素的构象和活性。

CRC critical reviews in biochemistry Pub Date : 1983-01-01 DOI: 10.3109/10409238309102793

V S Rao, T K Vasudevan

引用次数: 13