Alternative therapies in health and medicine最新文献

{"title":"Assessment of the Significance and Clinical Implications of Stress Hormones in Acute Pressure Injuries Among Trauma Patients.","authors":"Haishuang Wang, Hongbo Xu, Huanle Shu, Shi Xu, Wei Wang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>This study examines the dynamic changes of stress hormones, including insulin (INS), fasting blood glucose (FBG), glucagon (Glu), and cortisol (Cort), in trauma patients. By monitoring these changes and observing acute pressure injury (API) occurrences on the skin, the research analyzes the influence of stress hormones on API development in trauma patients.</p><p><strong>Methods: </strong>A prospective analysis involved 218 trauma patients admitted to a grade III-A general hospital in Wenzhou from April 2021 to June 2023. Among them, 44 cases developed API (API group), and 174 cases did not (control group). Levels of INS, Cort, Glu, and FBG were measured in both groups. Additionally, Abbreviated Injury Scale-Injury Severity Score (AIS-ISS) surveys and API severity assessments were conducted. Correlations between stress hormone levels and AIS-ISS were discussed. The predictive effects of AIS-ISS and stress hormones on API occurrence in trauma patients were analyzed using receiver operating characteristic (ROC) curves. The relationship between stress hormone levels and API severity was also observed.</p><p><strong>Results: </strong>Study's outcomes indicated distinct relationships between stress hormone levels and API occurrence in trauma patients. Specifically, INS demonstrated a negative correlation with AIS-ISS, highlighting its potential as a significant factor. Glu, Cort, and FBG revealed positive associations, emphasizing their roles in influencing API development (P < .05). The diagnostic efficacy of stress hormones in predicting API occurrence, as represented by the Area Under Curve (AUC) = 0.8100. Notably, within the API group, INS levels demonstrated a decline with worsening API. Conversely, Glu, Cort, and FBG exhibited increases in tandem with the aggravation of API symptoms (P < .05).</p><p><strong>Conclusions: </strong>This research suggests that assessing stress hormone levels in clinical settings can effectively predict API occurrence. Early testing could aid in the development of preventive or intervention measures, reducing the incidence and harm of API in trauma patients.</p>","PeriodicalId":7571,"journal":{"name":"Alternative therapies in health and medicine","volume":" ","pages":"128-133"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140317608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of Breast-Conserving Surgery Versus Modified Radical Surgery in the Treatment of Early Breast Cancer: A Meta-Analysis.","authors":"Tao Li, Peng Zhang, Yantao Zhang, Jun Qi, Yong Sun, Bin Lu","doi":"","DOIUrl":"","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;The study aims to assess and compare the outcomes of modified radical surgery, preserving the nipple-areola complex, against radical mastectomy in patients with triple-negative breast cancer. Emphasis is placed on the clinical significance of this comparison, including its potential impact on patient outcomes, quality of life, and healthcare resources.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Relevant literature from January 2017 to January 2022 was searched in the following databases: PubMed, Embase, MEDLINE, Science Citation Index, Web of Science, China National Knowledge Internet, CCD, and CSPD with keywords. The electric search yielded 613 relevant articles, including 351 from Pubmed, 187 from Embase, 5 from MEDLINE, 21 from CNKI, 4 from CCD, and 45 from CSPD. After duplicate screening, 185 items were eliminated, leaving 428 articles. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated with RevMan 5.3, and sensitivity analysis, cumulative meta-analysis, and publication bias analysis were also performed. A total of 613 relevant articles were collected from the above databases, among which 428 articles remained after the initial screening and were further screened based on the established inclusion and exclusion criteria. The efficacy of modified radical surgery and breast-conserving surgery in the treatment of early breast cancer was assessed by analyzing outcome indicators, including recurrence rate, distant metastatic rate, and three-year survival rate. The methods section details a systematic approach to data collection and analysis, specifying the databases and time frame for the literature search and the statistical tools used for the meta-analysis. The selection process, from the initial number of articles to the final inclusion based on defined criteria, is transparent, ensuring the study's methodological robustness in evaluating the efficacy of surgeries for early breast cancer.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Finally, ten articles were found to match the criteria and included in this study. According to the meta-analysis, there was no statistically significant difference between the breast-conserving therapy (BCT) and modified radical mastectomy (MRM) groups in terms of the recurrence rate (OR = 0.76, 95%CI = 0.39, 1.55, P &gt; .05) and distant metastatic rate (OR = 0.81, 95%CI = 0.46, 1.31, P &gt; .05). Nevertheless, the three-year survival rate was 85.2% in the BCT group and 91.7% in the MRM group; a statistically significant difference was observed in the three-year survival rate (OR = 1.47, 95%CI = 1.01, 2.37, P = .03) between the BCT and MRM groups. Accordingly, breast-conserving surgery and modified radical surgery produced comparable clinical outcomes for the treatment of early breast cancer.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;In the treatment of early breast cancer, breast-conserving surgery has the advantages of less bleeding, fewer clinical complications, and favorable cosmetic outcomes compa","PeriodicalId":7571,"journal":{"name":"Alternative therapies in health and medicine","volume":" ","pages":"238-245"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140847034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Caregiver Burden and Influencing Factors in Autologous Hematopoietic Stem Cell Transplantation Patients with Bendamustine Preconditioning.","authors":"Yanping Chen, Yan Zhang, Qianqian Chen","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>This paper comprehensively analyzes the caregiver burden and its influencing factors on primary caregivers in autologous hematopoietic stem cell transplantation (Auto-HSCT) with bendamustine preconditioning. Auto-HSCT refers to the transplantation of cells back to the patient, aiming to eliminate tumor cells and prolong the patient's life. Bendamustine, while enhancing the success rate of transplantation, has drawn considerable attention to the primary caregivers of patients. Due to the complex nature of the transplantation process, patients have diverse caregiving needs, which caregivers must address to support the entire treatment journey. The caregiver burden of primary caregivers is influenced by various factors, including the patient's disease condition, various aspects of the caregiver as an individual, and psychological factors. The article emphasizes the need for personalized care plans and psychological support to minimize caregiver burden and improve overall quality of life. This study has positive implications for optimizing the implementation of Auto-HSCT therapy.</p>","PeriodicalId":7571,"journal":{"name":"Alternative therapies in health and medicine","volume":" ","pages":"246-250"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140848395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of the Nursing Effect of Hemiplegic Limb Rehabilitation Training in the Care of Stroke Patients.","authors":"Yafen Cao, Simin Chen, Xuejuan Cheng, Rui Ji","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>The objective of this study was to examine the impact of hemiplegic limb rehabilitation training on the nursing care of individuals who have experienced a stroke.</p><p><strong>Methods: </strong>Seventy-six stroke patients from Zhangjiagang Hospital of Traditional Chinese Medicine participated in a study comparing the effects of different nursing interventions. The patients were divided into 2 groups: a control that received standard nursing care and an observation group that received rehabilitation training in addition to standard care. The researchers analyzed the effects on limb movement, daily activities, muscle recovery, and nursing satisfaction between the 2 groups.</p><p><strong>Results: </strong>The nursing intervention in the observation group showed a significantly higher effectiveness rate of 94.74% compared to the control group's rate of 55.26% (P < .05). Prior to the intervention, there was no notable difference in limb movement scores between the 2 groups (P > .05). Similarly, there was no significant difference in Barthel Index scores between the 2 groups before the intervention (P > .05). However, after the intervention, the Bathel Index score in the observation group was significantly higher than that of the control group (P < .05).</p><p><strong>Conclusion: </strong>Hemiplegic limb rehabilitation training nursing care positively impacts stroke patients, improving physical mobility, muscle strength, and overall quality of life. This method should be prioritized and implemented in clinical practice.</p>","PeriodicalId":7571,"journal":{"name":"Alternative therapies in health and medicine","volume":" ","pages":"408-413"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140850141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Application Effect of Evidence-Based Targeted Nursing in Severe Preeclamptic Women and Its Impact on Maternal Psychological Status, Quality of Life, and Maternal-Infant Outcomes.","authors":"Wei Yan, Ying Zhou","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To analyze the application effect of evidence-based targeted nursing in severe preeclamptic women and its impact on maternal psychological status, quality of life, and maternal-infant outcomes.</p><p><strong>Methods: </strong>A retrospective analysis was conducted on clinical data of 97 severe preeclamptic patients admitted to our hospital from June 2021 to June 2023. All patients met the complete inclusion and exclusion criteria. Based on the different nursing intervention plans received by the patients, they were divided into a control group (n=47) and an observation group (n=50). Patients in the control group received routine nursing intervention, while patients in the observation group received evidence-based targeted nursing. A comparison was made between the two groups in terms of levels of psychological status indicators, quality of life, maternal pregnancy outcomes, neonatal outcomes, Apgar scores, and nursing satisfaction.</p><p><strong>Results: </strong>(1) Psychological status indicators: Before the intervention, the two groups had no significant difference in EPDS scores and SAS scores (P > .05). After the intervention, the EPDS scores and SAS scores in the observation group were significantly lower than those in the control group (P < .05, effect size d = 0.65 for EPDS scores and d = 0.72 for SAS scores), indicating a substantial reduction in depression and anxiety levels. (2) Quality of life: Before the intervention, there was no significant difference in the scores for health status, physiological function, and mental status between the two groups (P > .05). After the intervention, the scores for health status, physiological function, and mental status in the observation group were significantly higher than in the control group (P < .05, effect size d = 0.58 for health status, d = 0.63 for physiological function, and d = 0.61 for mental status), suggesting a notable improvement in the overall quality of life for patients. (3) Maternal pregnancy outcomes: The incidence of adverse pregnancy outcomes in the control group was 42.55%, while in the observation group, it was 18.00%. The incidence of adverse pregnancy outcomes in the observation group was significantly lower than in the control group (P < .05, effect size d = 0.82), indicating a substantial reduction in adverse outcomes. (4) Neonatal outcomes and Apgar scores: The incidence of adverse neonatal outcomes in the control group was 46.81%, with an Apgar score of (7.13±1.05), while in the observation group, it was 22.00%, with an Apgar score of (7.96±1.17). The incidence of adverse neonatal outcomes in the observation group was significantly lower, and the Apgar scores were significantly higher than those in the control group (P < .05, effect size d = 0.73 for adverse neonatal outcomes, and d = 0.68 for Apgar scores), indicating improved neonatal outcomes. (5) Nursing satisfaction: The nursing satisfaction in the control group was 80.85%, whereas i","PeriodicalId":7571,"journal":{"name":"Alternative therapies in health and medicine","volume":" ","pages":"414-421"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140848936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Influence of CD40/CD40L, MMP2/MMP9 on Uterine Artery Blood Flow and Their Expression in Recurrent Miscarriage.","authors":"Huirong Li, Liang Ding, Jian Ding, Fudan Huang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>This study aims to investigate the expression levels of soluble CD40L (sCD40L), matrix metalloproteinase 2 (MMP2), and matrix metalloproteinase 9 (MMP9) in the serum of patients experiencing recurrent abortion and their impact on uterine artery blood flow.</p><p><strong>Methods: </strong>A cohort of 200 patients with recurrent abortion was selected for this investigation. The levels of sCD40L, MMP2, and MMP9 in serum were assessed using ELISA, while ultrasound was employed to measure the pulsatility index (PI) and resistance index (RI) in uterine artery blood flow. Pregnancy outcomes were observed, and the expression of CD40/CD40L and MMP2/MMP9 in villi tissues was compared between patients experiencing recurrent abortion failure and those with normal pregnancies.</p><p><strong>Results: </strong>In the successful pregnancy group of patients with recurrent spontaneous abortion (RSA), serum levels of sCD40L, MMP2, and MMP9 were significantly lower than those in the failed pregnancy group. Additionally, both RI and PI were notably reduced. The expression of each gene showed a correlation with RI and PI. Furthermore, the expression levels of CD40, CD40L, MMP2, and MMP9 in the pregnancy failure group were significantly higher than in the normal voluntary termination group.</p><p><strong>Conclusion: </strong>Serum levels of sCD40L, MMP2, and MMP9, along with non-invasive and easily accessible indicators such as PI and RI in uterine artery blood flow measured by ultrasound, emerge as potential predictive markers for the outcome of recurrent miscarriage pregnancies. Moreover, these indicators can serve as valuable evaluation markers in clinical practice, facilitating the monitoring of treatment effectiveness for recurrent miscarriage.</p>","PeriodicalId":7571,"journal":{"name":"Alternative therapies in health and medicine","volume":" ","pages":"222-226"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140849004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Effects of the Unani Medicine Formulations Majoon and Tila Baras-e-Aswad on Plaque Psoriasis: A Single-Arm Clinical Study.","authors":"Abdul Mobeen, Faisel Manzoor","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Background: </strong>Psoriasis is a chronic, autoimmune, T-cell-mediated inflammatory disease with an \"onset-remission-recurrence\" pattern, and is managed symptomatically, therefore the need for treatment is usually lifelong.</p><p><strong>Primary study objective: </strong>This study aimed to evaluate the efficacy of Majoon and Tila Baras-e-Aswad, two combination formulations, consisting of herbal, mineral, and animal drugs (antioxidants, anti-psoriatic, antihistaminic, antipruritic, and skin protection with healing activities), prescribed for the treatment of Baras-e-Aswad, having a plaque psoriasis-like diagnosis in Unani medicine.</p><p><strong>Methods/design: </strong>Open-label single-arm and single-center clinical study with pre-and post-treatment analysis.</p><p><strong>Setting: </strong>Conducted in National Institute of Unani Medicine (NIUM) Hospital, Bangalore, India.</p><p><strong>Participants: </strong>A total of 36 patients with plaque psoriasis with mild to moderate severity and aged 18-60 were included.</p><p><strong>Interventions: </strong>The intervention formulation Majoon 10 g orally and Tila Baras-e-Aswad topically was given for 45 days. Primary Outcome Measures: Evaluation of prognosis with averaged PASI (Psoriasis Area and Severity Index) (EPAP) and dermatology life quality index (DLQI) were the outcome of the study and analyzed per protocol.</p><p><strong>Results: </strong>The data analysis of 30 patients who completed the study showed a statistically significant result in the EPAP scores (P < .05), which reduced from 1112.57 ± 988.45 at baseline to 1108.95 ± 988.87, 1107.71 ± 988.52, and 1105.57 ± 988.76 on 15th, 30th, and 45th day respectively. DLQI score reduced significantly (P < .05) from 18.60 ± 2.72 at baseline to 15.20 ± 2.90, 10.76 ± 2.07, and 8.00 ± 2.43 on 15th, 30th, and 45th day respectively. The test drugs were found to be tolerable without any adverse effects.</p><p><strong>Conclusion: </strong>This study highlights the positive effect of Majoon and Tila Baras-e-Aswad combination formulation on the clinical efficacy, quality of life, and short-term prognosis in chronic plaque psoriasis patients, providing valuable insights for further research in a well-designed study.</p>","PeriodicalId":7571,"journal":{"name":"Alternative therapies in health and medicine","volume":" ","pages":"22-27"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142680388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcranial Direct Current Stimulation Combined with Stabilization Exercises in Chronic Neck Pain: A Randomized Controlled Trial.","authors":"Tuğba Dere, Hanife Abakay, Nazmi Saritaş, Ayşe Güç, Rabia Uğur, Fatma Eda Ekşi, Kübra Seyrek","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>The study aimed to investigate the effect of Transcranial Direct Current Stimulation combined with cervical stabilization exercises on functional status, cognitive functions, and sleep quality in individuals with chronic neck pain.</p><p><strong>Materials and methods: </strong>The study involved 29 individuals (mean age 33.06 ± 14.81 years). The experimental group (n=10) received combined Transcranial Direct Current Stimulation (2 mA/20 min) with cervical stabilization exercises, while the control group (n=10) received only cervical stabilization exercises. The sham group (n=9) received placebo Transcranial Direct Current Stimulation (0 mA/20 min) with cervical stabilization exercises. Assessments were conducted before and after completing 8 weeks with 2 days per week of intervention, i.e., 16 sessions.</p><p><strong>Primary outcome measures: </strong>Pain severity was assessed using the 10 cm Visual Analogue Scale and the Neck Bournemouth Questionnaire. Muscle endurance was measured by testing cervical flexors, extensors, and deep flexor muscles. Functional limitations were evaluated with the Profile Fitness Mapping neck questionnaire. Cognitive status was assessed using the Montreal Cognitive Assessment and the Trail Making Test. Sleep quality was evaluated with the Pittsburgh Sleep Quality Index.</p><p><strong>Results: </strong>After 16 sessions, all groups demonstrated a significant increase in cervical flexor muscle endurance (P < .05) and a significant decrease in Visual Analogue Scale pain scores at rest and at activity. Additionally, the experimental group exhibited a significant improvement in Visual Analogue Scale with activity, Profile Fitness Mapping neck questionnaire, and Montreal Cognitive Assessment scores. No significant difference was found between the groups in cognitive function or sleep quality (P > .05).</p><p><strong>Conclusion: </strong>The importance of Transcranial Direct Current Stimulation combined with cervical stabilization exercises as a tool for pain management and rehabilitation of impaired motor control in cervical muscles in individuals with chronic neck pain is emphasized. It is suggested that combined approaches are considered instead of cervical stabilization exercises alone.</p>","PeriodicalId":7571,"journal":{"name":"Alternative therapies in health and medicine","volume":" ","pages":"36-44"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142680553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Analysis of Clinical Outcomes: Posterior Cervical Endoscopic Discectomy versus Fenestration Laminectomy Discectomy for Cervical Disc Herniation.","authors":"Minhong Zhou, Linxia Wang, Xixi Xu, Lijuan Wang, Peifang Wang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>It aimed to investigate the difference in clinical efficacy between posterior cervical endoscopic discectomy (PCED) and Fenestration laminectomy discectomy (FLD) in cervical disc herniation (CDH).</p><p><strong>Methods: </strong>This retrospective study analyzed 100 CDH patients undergoing nucleotomy and assigned them into the FLD and PCED groups, 50 cases for each group. The differences in operation time, intraoperative blood loss, skin incision, off-bed time, and hospital stay were evaluated. Numeric rating scales (NRS), Oswestry disability Index (ODI), Japanese Orthopaedic Association (JOA), excellent and good clinical efficacy, quality of life (QoL) SF-36 score, and complication rate were compared.</p><p><strong>Results: </strong>The results showed that compared with the FLD group, the PCED group had increased operation time, decreased intraoperative blood loss, skin incision length, off-bed time, and hospital stay (P < .01). Compared with the FLD group, the PCED group had decreased NRS and ODI scores and increased JOA scores at 1 d, 3 d, 1 month, 3 months, 6 months, 12 months, and 24 months after operation (P < .05). Compared with the FLD group, the excellent and good rate of the PCED group increased significantly after 6 months, 1 year, and 2 years (52.0% vs 64.0%, 58.0% vs. 80.0%, 68.0% vs 90.0%, P < .05). Relative to the FLD group, the physical function, emotional function, vitality, social function, and mental health score of the PCED group increased obviously at 2 years after operation (P < .01). The postoperative complication rate was 0% in both FLD and PCED groups. PCED has good long-term clinical efficacy in the treatment of CDH, with excellent recovery and high safety.</p><p><strong>Conclusion: </strong>PCED showed favorable long-term clinical efficacy in the treatment of CDH, with excellent recovery and high safety. Compared to FLD, PCED resulted in reduced intraoperative blood loss, shorter incision length, and faster recovery. It also led to improved pain scores, functional outcomes, and quality of life measures. The absence of postoperative complications further supports the use of PCED as an effective treatment option for CDH.</p>","PeriodicalId":7571,"journal":{"name":"Alternative therapies in health and medicine","volume":" ","pages":"65-71"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140850065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vitro Culture and Biological Characterization of Meniscal Fibrochondrocytes.","authors":"Zhigang Zhou, Yuxin Shen","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Background: </strong>Currently, there is a lot of discussion in clinical and scientific research over the best ways to delay meniscal degeneration and speed up its healing. Meniscal injury and degeneration are significant contributors to the development of knee osteoarthritis in a pathological state.</p><p><strong>Objective: </strong>To isolate, culture and characterize rat meniscal fibrochondrocytes in vitro, and to provide a simple and feasible cell culture method for the study of damage repair of rat meniscal fibrochondrocytes.</p><p><strong>Methods: </strong>The rat medial and lateral meniscus of both knees was surgically isolated. Trypsin and type II collagenase were used to remove the cells, and toluidine blue staining and type II collagen immunofluorescence were used to identify the cells. The cells were then routinely cultured in low-sugar DMEM complete culture medium.</p><p><strong>Results: </strong>At different time points, cells showed different physiological shapes, from polygonal or short spindle to spindle shape, and finally to triangle or ellipse, and cell proliferation ability gradually increased with time. The OD values of cells cultured at 48h and 72h were higher than those at 24h. Comparing OD values of cells cultured for 48h and 72h, although OD value of 72h increased. Toluidine blue staining and type I collagen immunofluorescence staining were positive.</p><p><strong>Conclusion: </strong>A more dependable technique for fibrochondrocyte isolation and culture is offered for the study of meniscus in molecular biology and tissue engineering. The cells cultured using this method are morphologically stable, have a strong proliferation ability, and possess the fundamental biological properties of fibrochondrocytes in vivo.</p>","PeriodicalId":7571,"journal":{"name":"Alternative therapies in health and medicine","volume":" ","pages":"366-371"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140852952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}