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Changes in tropomyosin during primary culture of embryonic myocardiocytes 胚胎心肌细胞原代培养过程中原肌球蛋白的变化
Cell biology international reports Pub Date : 1992-05-01 DOI: 10.1016/S0309-1651(06)80064-0
M.A. Muros, A.E. Aránega, C. Vélez, F.J. González, J.E. Fernández, L. Alvarez, A. Aránega
{"title":"Changes in tropomyosin during primary culture of embryonic myocardiocytes","authors":"M.A. Muros,&nbsp;A.E. Aránega,&nbsp;C. Vélez,&nbsp;F.J. González,&nbsp;J.E. Fernández,&nbsp;L. Alvarez,&nbsp;A. Aránega","doi":"10.1016/S0309-1651(06)80064-0","DOIUrl":"10.1016/S0309-1651(06)80064-0","url":null,"abstract":"<div><p>We chose the Hamburger and Hamilton's stage 29 (HH 29) to investigate the expression of tropomyosin in chick myocardiocytes during 14 days on culture. Throughout 14 days of cell culture, changes in cell morphology were accompained by a redistribution of tropomyosin in different cell compartments. We used FACScan, SDS-PAGE and densitometric analysis to quantify total cell tropomyosin and concentrations of this protein in different cell fractions. Tropomyosin was found mostly in the cytoskeletal fraction than in the cytoplasmic.</p><p>When we compared the densitometric values from SDS-PAGE of cells in different stages of development we found that in HH 19, tropomyosin was more abundant in the cytoplasmic than in the cytoskeletal fraction. By HH 29, the two fractions had become inverted, and in HH 39, tropomyosin was clearly more abundant in the cytoskeletal than in the cytoplasmic fraction.</p><p>In the IFI analysis, tropomyosin was found to label the Stress fiberlike structures (SFL) in different patterns depending on the area of the cell which expressed this protein.</p></div>","PeriodicalId":75683,"journal":{"name":"Cell biology international reports","volume":"16 5","pages":"Pages 451-464"},"PeriodicalIF":0.0,"publicationDate":"1992-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0309-1651(06)80064-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12794215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Thapsigargin, an inhibitor of Ca2+-ATPase, antagonizes vacuole formation of hepatoma cells induced by teleocidin Thapsigargin是一种Ca2+- atp酶抑制剂，可拮抗远杀素诱导的肝癌细胞液泡形成
Cell biology international reports Pub Date : 1992-05-01 DOI: 10.1016/S0309-1651(06)80069-X
Ayumi Tsukamoto, Yoshiyasu Kaneko, Kiyoshi Kurokawa
{"title":"Thapsigargin, an inhibitor of Ca2+-ATPase, antagonizes vacuole formation of hepatoma cells induced by teleocidin","authors":"Ayumi Tsukamoto,&nbsp;Yoshiyasu Kaneko,&nbsp;Kiyoshi Kurokawa","doi":"10.1016/S0309-1651(06)80069-X","DOIUrl":"10.1016/S0309-1651(06)80069-X","url":null,"abstract":"","PeriodicalId":75683,"journal":{"name":"Cell biology international reports","volume":"16 5","pages":"Pages 491-492"},"PeriodicalIF":0.0,"publicationDate":"1992-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0309-1651(06)80069-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12526086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
The interaction of staphylococcal protein a with free coelomocytes of annelids 葡萄球菌蛋白a与环节动物游离腔胚细胞的相互作用
Cell biology international reports Pub Date : 1992-05-01 DOI: 10.1016/S0309-1651(06)80067-6
Martin Bilej, Jaroslav Rejnek, Ludmila Tučková
{"title":"The interaction of staphylococcal protein a with free coelomocytes of annelids","authors":"Martin Bilej,&nbsp;Jaroslav Rejnek,&nbsp;Ludmila Tučková","doi":"10.1016/S0309-1651(06)80067-6","DOIUrl":"10.1016/S0309-1651(06)80067-6","url":null,"abstract":"<div><p>The Staphylococcal protein A (SpA) binding protein was detected on the surface of annelid coelomocytes. The flow cytometric analysis revealed that 50 % coelomocytes of <em>Lumbricus terrestris</em> react with SpA, a figure six times higher than the number of positive coelomocytes found in <em>Eisenia foetida</em>.</p></div>","PeriodicalId":75683,"journal":{"name":"Cell biology international reports","volume":"16 5","pages":"Pages 481-485"},"PeriodicalIF":0.0,"publicationDate":"1992-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0309-1651(06)80067-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12794747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
LM Franks和NM Teich，编辑，介绍癌症的细胞和分子生物学(第二版)，牛津大学出版社，剑桥(1991)ISBN 0 19 854734 X。
Cell biology international reports Pub Date : 1992-04-01 DOI: 10.1016/S0309-1651(06)80147-5
P Monaghan
{"title":"","authors":"P Monaghan","doi":"10.1016/S0309-1651(06)80147-5","DOIUrl":"10.1016/S0309-1651(06)80147-5","url":null,"abstract":"","PeriodicalId":75683,"journal":{"name":"Cell biology international reports","volume":"16 4","pages":"Page 387"},"PeriodicalIF":0.0,"publicationDate":"1992-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0309-1651(06)80147-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56257237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Constituent cell biology societies 组成细胞生物学学会
Cell biology international reports Pub Date : 1992-04-01 DOI: 10.1016/S0309-1651(06)80149-9
{"title":"Constituent cell biology societies","authors":"","doi":"10.1016/S0309-1651(06)80149-9","DOIUrl":"https://doi.org/10.1016/S0309-1651(06)80149-9","url":null,"abstract":"","PeriodicalId":75683,"journal":{"name":"Cell biology international reports","volume":"16 4","pages":"Pages 392-395"},"PeriodicalIF":0.0,"publicationDate":"1992-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0309-1651(06)80149-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"137195781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of spermidine synthase inhibition in cultured chick embryo fibroblasts 抑制鸡胚成纤维细胞亚精胺合酶的作用
Cell biology international reports Pub Date : 1992-04-01 DOI: 10.1016/S0309-1651(06)80140-2
A. Caruso , A. Pellati , P. Bosi , N. Arena , G. Stabellini
{"title":"Effects of spermidine synthase inhibition in cultured chick embryo fibroblasts","authors":"A. Caruso ,&nbsp;A. Pellati ,&nbsp;P. Bosi ,&nbsp;N. Arena ,&nbsp;G. Stabellini","doi":"10.1016/S0309-1651(06)80140-2","DOIUrl":"10.1016/S0309-1651(06)80140-2","url":null,"abstract":"<div><p>The administration of bis-cyclohexylammonium sulfate (BCHS), a spermidine synthase inhibitor, to <u>in vitro</u> cultures of chick embryo fibroblasts caused a decrease in cellular spermidine levels and an increase in putrescine and spermine. Cell proliferation rate and DNA synthesis were also inhibited. As protein synthesis did not change, it would seem that low levels of cellular spermidine inhibit cell growth depressing DNA synthesis.</p></div>","PeriodicalId":75683,"journal":{"name":"Cell biology international reports","volume":"16 4","pages":"Pages 349-358"},"PeriodicalIF":0.0,"publicationDate":"1992-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0309-1651(06)80140-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12695143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Meetings and conferences 会议和会议
Cell biology international reports Pub Date : 1992-04-01 DOI: 10.1016/S0309-1651(06)80148-7
{"title":"Meetings and conferences","authors":"","doi":"10.1016/S0309-1651(06)80148-7","DOIUrl":"https://doi.org/10.1016/S0309-1651(06)80148-7","url":null,"abstract":"","PeriodicalId":75683,"journal":{"name":"Cell biology international reports","volume":"16 4","pages":"Pages 388-391"},"PeriodicalIF":0.0,"publicationDate":"1992-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0309-1651(06)80148-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"137195780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Proliferation-dependency of γ-glutamyl hydrolase activity in various mouse cells γ-谷氨酰水解酶活性在多种小鼠细胞中的增殖依赖性
Cell biology international reports Pub Date : 1992-04-01 DOI: 10.1016/S0309-1651(06)80142-6
Ewa Sikora, Bozena Kaminska, Barbara Grzelakowska-Sztabert
{"title":"Proliferation-dependency of γ-glutamyl hydrolase activity in various mouse cells","authors":"Ewa Sikora,&nbsp;Bozena Kaminska,&nbsp;Barbara Grzelakowska-Sztabert","doi":"10.1016/S0309-1651(06)80142-6","DOIUrl":"10.1016/S0309-1651(06)80142-6","url":null,"abstract":"<div><p>The activity of γ-glutamyl hydrolase, the enzyme which deglutamylates folyl and antifolyl polyglutamates, changed significantlly in mouse cells during different phases of growth, being about two times lower in actively proliferating mice splenocytes and fibroblasts than in nondividing cells. In EAC cells growing in vivo the lowest activity was observed in cells in the logarythmic phase. Methotrexate treatment of mice in a dose of 500 mg/kg body weight increased the activity of the enzyme in EAC cells about 1.5 times. We suggest that γ-glutamyl hydrolase is a proliferating dependent enzyme which together with folylpolyglutamate synthetase ensures in cells an appriopriate amount of folates in the form of polyglutamates necessary for optimizing folate-dependent biosynthetic activities.</p></div>","PeriodicalId":75683,"journal":{"name":"Cell biology international reports","volume":"16 4","pages":"Pages 369-375"},"PeriodicalIF":0.0,"publicationDate":"1992-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0309-1651(06)80142-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12695145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Expression of SV40 T-antigen in lipoma cells with a chromosomal translocation T(3;12) is not sufficient for direct immortalization 在染色体易位T的脂肪瘤细胞中表达SV40 T抗原(3;12)并不足以实现直接永生化
Cell biology international reports Pub Date : 1992-04-01 DOI: 10.1016/S0309-1651(06)80139-6
Gazanfer Belge, Bernd Kazmierczak, Kerstin Meyer-Bolte, Sabine Bartnitzke, Jörn Bullerdiek
{"title":"Expression of SV40 T-antigen in lipoma cells with a chromosomal translocation T(3;12) is not sufficient for direct immortalization","authors":"Gazanfer Belge,&nbsp;Bernd Kazmierczak,&nbsp;Kerstin Meyer-Bolte,&nbsp;Sabine Bartnitzke,&nbsp;Jörn Bullerdiek","doi":"10.1016/S0309-1651(06)80139-6","DOIUrl":"10.1016/S0309-1651(06)80139-6","url":null,"abstract":"<div><p>12q13-15 changes are the most frequent cytogenetic abnormalities in human tumor cells. To test their biological significance we used an assay based on lipoma cells with a limited in vitro lifetime and this type of chromosomal aberration. Lipoma cells with a reciprocal translocation t(3;12)(q28;q14) were transformed by transfection with a plasmid containing the SV40 “early region”. The transformed cells showed an altered morphology with loss of contact inhibition, formation of foci, and T-antigen expression. They were immortalized after a growth crisis. The karyotypic patterns before and after the crisis show that the translocation together with expression of SV40 T-antigen is not sufficient for direct immortalization.</p></div>","PeriodicalId":75683,"journal":{"name":"Cell biology international reports","volume":"16 4","pages":"Pages 339-347"},"PeriodicalIF":0.0,"publicationDate":"1992-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0309-1651(06)80139-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12500062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 19
Cell biology international reports Pub Date : 1992-04-01 DOI: 10.1016/S0309-1651(06)80145-1
GRTB
{"title":"","authors":"GRTB","doi":"10.1016/S0309-1651(06)80145-1","DOIUrl":"10.1016/S0309-1651(06)80145-1","url":null,"abstract":"","PeriodicalId":75683,"journal":{"name":"Cell biology international reports","volume":"16 4","pages":"Page 384"},"PeriodicalIF":0.0,"publicationDate":"1992-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0309-1651(06)80145-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"112026038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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