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Influence of low density lipoproteins on vascular smooth muscle cell growth and motility: Modulation by extracellular matrix 低密度脂蛋白对血管平滑肌细胞生长和运动的影响:细胞外基质的调节
Cell biology international reports Pub Date : 1992-05-01 DOI: 10.1016/S0309-1651(06)80063-9
Sandra Harris-Hooker , Gary L. Sanford , Vergena Montgomery , Ricardo Rivers , Nerimiah Emmett
{"title":"Influence of low density lipoproteins on vascular smooth muscle cell growth and motility: Modulation by extracellular matrix","authors":"Sandra Harris-Hooker ,&nbsp;Gary L. Sanford ,&nbsp;Vergena Montgomery ,&nbsp;Ricardo Rivers ,&nbsp;Nerimiah Emmett","doi":"10.1016/S0309-1651(06)80063-9","DOIUrl":"10.1016/S0309-1651(06)80063-9","url":null,"abstract":"<div><p>Low density lipoproteins (LDL) are thought to play a major role in cardiovascular diseases such as atherosclerosis. Much remains to be done to understand the cellular effects of LDL and how the extracellular matrix (ECM) influences these effects. We found that LDL produced a dose dependent increase in vascular smooth muscle cell (SMC) proliferation. The ECM altered the proliferative response of SMC to LDL: on collagen I there was a 66% inhibition, endothelial cell derived-ECM a 2-fold increase, and collagen IV no difference in proliferation compared to paired controls. LDL affected SMC motility (cell area and shape factor) but the extent and direction of the effect depended on whether the cells were cultured on uncoated or coated dishes. LDL treated cultures had a 5-fold lower migration rate but net movement was not different, suggesting that LDL decreased SMC random movement. There was a dose-dependent accumulation of lipid by SMC incubated with LDL and, subsequently, cytoplasmic lipid droplets were observed. Cells cultured on uncoated plates showed an increased cholesterol content as a function of LDL concentration. In contrast, cells cultured on a collagen IV matrix showed no net change in cholesterol content over the range of LDL concentrations studied. Hence, the uptake of LDL cholesterol appears to be completely inhibited by this matrix. These studies indicate that the influence of LDL on several SMC parameters is modulated by ECM components.</p></div>","PeriodicalId":75683,"journal":{"name":"Cell biology international reports","volume":"16 5","pages":"Pages 433-450"},"PeriodicalIF":0.0,"publicationDate":"1992-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0309-1651(06)80063-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12794214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 17
A cell surface antigen associated with meiosis in male Staurdoderus scalaris(orthopteran) 一种与雄性鳞叔翅虫减数分裂相关的细胞表面抗原
Cell biology international reports Pub Date : 1992-05-01 DOI: 10.1016/S0309-1651(06)80060-3
Melanie J. Percy, Hugh L. Fletcher, Alan Trudgett
{"title":"A cell surface antigen associated with meiosis in male Staurdoderus scalaris(orthopteran)","authors":"Melanie J. Percy,&nbsp;Hugh L. Fletcher,&nbsp;Alan Trudgett","doi":"10.1016/S0309-1651(06)80060-3","DOIUrl":"10.1016/S0309-1651(06)80060-3","url":null,"abstract":"<div><p>Immunofluorescence has identified seven monoclonal antibodies reactive with the surface of meiotic cells and absent in premeiotic cells. Analysis by immunogold electron microscopy indicated that these antigens were present on the external surface of the cells and were coincident with the presence of synaptonemal complexes in the nucleus. On immunoblots a common glycosylated protein of 205 kDa was recognized, in addition to smaller subunits, suggesting the presence of a protein complex comprised of smaller peptides.</p></div>","PeriodicalId":75683,"journal":{"name":"Cell biology international reports","volume":"16 5","pages":"Pages 407-413"},"PeriodicalIF":0.0,"publicationDate":"1992-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0309-1651(06)80060-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12794213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Meetings and conferences 会议和会议
Cell biology international reports Pub Date : 1992-05-01 DOI: 10.1016/S0309-1651(06)80073-1
{"title":"Meetings and conferences","authors":"","doi":"10.1016/S0309-1651(06)80073-1","DOIUrl":"https://doi.org/10.1016/S0309-1651(06)80073-1","url":null,"abstract":"","PeriodicalId":75683,"journal":{"name":"Cell biology international reports","volume":"16 5","pages":"Pages 499-502"},"PeriodicalIF":0.0,"publicationDate":"1992-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0309-1651(06)80073-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"137155019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Microscopic Anatomy of Invertebrates, Vols. 1–3 《无脊椎动物的显微解剖学》1-3卷
Cell biology international reports Pub Date : 1992-05-01 DOI: 10.1016/S0309-1651(06)80070-6
I.D. Bowen
{"title":"Microscopic Anatomy of Invertebrates, Vols. 1–3","authors":"I.D. Bowen","doi":"10.1016/S0309-1651(06)80070-6","DOIUrl":"10.1016/S0309-1651(06)80070-6","url":null,"abstract":"","PeriodicalId":75683,"journal":{"name":"Cell biology international reports","volume":"16 5","pages":"Pages 493-495"},"PeriodicalIF":0.0,"publicationDate":"1992-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0309-1651(06)80070-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56257067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Homologous antigen for T cell receptor in axial organ cells from the asterid Asterias rubens 星形星轴器官细胞T细胞受体的同源抗原
Cell biology international reports Pub Date : 1992-05-01 DOI: 10.1016/S0309-1651(06)80068-8
Michel Leclerc, M. Bajelan
{"title":"Homologous antigen for T cell receptor in axial organ cells from the asterid Asterias rubens","authors":"Michel Leclerc,&nbsp;M. Bajelan","doi":"10.1016/S0309-1651(06)80068-8","DOIUrl":"10.1016/S0309-1651(06)80068-8","url":null,"abstract":"<div><p>The axial organ (A.O. cells) of the star <em>Asterias rubens</em> is a primitive immune organ. The total population was fractionated into two populations: adherent (B-like) and non-adherent (T-like) to nylon wool. The adherent cells resemble mammalian B lymphocytes and bear homologous human T cell receptor (β chain) to a higher degree than T-like cells which resemble T lymphocytes.</p></div>","PeriodicalId":75683,"journal":{"name":"Cell biology international reports","volume":"16 5","pages":"Pages 487-490"},"PeriodicalIF":0.0,"publicationDate":"1992-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0309-1651(06)80068-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12794748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
High molecular weight microtubule-associated proteins within testes of hemipteran insects 半翅目昆虫睾丸内的高分子量微管相关蛋白
Cell biology international reports Pub Date : 1992-05-01 DOI: 10.1016/S0309-1651(06)80065-2
Cherryl Hunt, Howard Stebbings
{"title":"High molecular weight microtubule-associated proteins within testes of hemipteran insects","authors":"Cherryl Hunt,&nbsp;Howard Stebbings","doi":"10.1016/S0309-1651(06)80065-2","DOIUrl":"10.1016/S0309-1651(06)80065-2","url":null,"abstract":"<div><p>Results presented here indicate that the high molecular weight microtubule-associated proteins isolated from the ovaries of the hemipterans, <u>Oncopeltus fasciatus</u> and <u>Notonecta glauca</u>, while absent from nervous tissue, are present in the testes of the respective species. Here they are seen to be located to extensive microtubule aggregates within the cells surrounding the sperm, but not obviously to the sperm themselves.</p></div>","PeriodicalId":75683,"journal":{"name":"Cell biology international reports","volume":"16 5","pages":"Pages 465-474"},"PeriodicalIF":0.0,"publicationDate":"1992-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0309-1651(06)80065-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12794745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
The Practical Approach Series Cumulative Methods Index 实用方法系列累积方法索引
Cell biology international reports Pub Date : 1992-05-01 DOI: 10.1016/S0309-1651(06)80071-8
{"title":"The Practical Approach Series Cumulative Methods Index","authors":"","doi":"10.1016/S0309-1651(06)80071-8","DOIUrl":"https://doi.org/10.1016/S0309-1651(06)80071-8","url":null,"abstract":"","PeriodicalId":75683,"journal":{"name":"Cell biology international reports","volume":"16 5","pages":"Pages 496-497"},"PeriodicalIF":0.0,"publicationDate":"1992-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0309-1651(06)80071-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"137155007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Inhibition of production of monocyte/macrophage-derived angiogenic activity by oxygen free-radical scavengers 氧自由基清除剂对单核细胞/巨噬细胞衍生血管生成活性的抑制作用
Cell biology international reports Pub Date : 1992-05-01 DOI: 10.1016/S0309-1651(06)80061-5
Alisa E. Koch , Michael Cho , James C. Burrows , Peter J. Polverini , S. Joseph Leibovich
{"title":"Inhibition of production of monocyte/macrophage-derived angiogenic activity by oxygen free-radical scavengers","authors":"Alisa E. Koch ,&nbsp;Michael Cho ,&nbsp;James C. Burrows ,&nbsp;Peter J. Polverini ,&nbsp;S. Joseph Leibovich","doi":"10.1016/S0309-1651(06)80061-5","DOIUrl":"10.1016/S0309-1651(06)80061-5","url":null,"abstract":"<div><p>We showed previously that thiol-containing compounds inhibited the production of macrophage-mediated angiogenic activity. Since thiol-containing compounds may act on macrophages by affecting activation and inhibiting the production of oxygen free-radicals, we studied the effects of oxygen free-radical scavengers on production of angiogenic activity by elicited mouse peritoneal macrophages and lipopolysaccharide stimulated normal human monocytes. Monocyte/macrophage conditioned media were potently angiogenic when assayed in rat corneas, while conditioned media from oxygen free-radical scavenger-treated cells were not. The inhibitory effect of oxygen free-radical scavengers was due to a direct effect on monocyte/macrophage production of angiogenic activity but was not due solely to a decrease in the production of the macrophage-derived angiogenic cytokine tumor necrosis factor-α. We conclude that oxygen free-radical scavengers are potent inhibitors of the production of macrophage-mediated angiogenic activity.</p></div>","PeriodicalId":75683,"journal":{"name":"Cell biology international reports","volume":"16 5","pages":"Pages 415-425"},"PeriodicalIF":0.0,"publicationDate":"1992-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0309-1651(06)80061-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12551302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 29
Identification of a 5-HT1A receptor positively coupled to planarian adenylate cyclase 5-HT1A受体与涡虫腺苷酸环化酶正偶联的鉴定
Cell biology international reports Pub Date : 1992-05-01 DOI: 10.1016/S0309-1651(06)80062-7
P. Cretì , A. Capasso , M. Grasso , E. Parisi
{"title":"Identification of a 5-HT1A receptor positively coupled to planarian adenylate cyclase","authors":"P. Cretì ,&nbsp;A. Capasso ,&nbsp;M. Grasso ,&nbsp;E. Parisi","doi":"10.1016/S0309-1651(06)80062-7","DOIUrl":"10.1016/S0309-1651(06)80062-7","url":null,"abstract":"<div><p>The adenylate cyclase system present in particulate fractions prepared from two planarian species was tested for sensitivity to various neurotransmitters. While dopamine and other catecholamines were ineffective, serotonin was capable of stimulating the enzyme. Among the various serotonin agonists tested, only 8-OH-DPAT resulted effective on the adenylate cyclase activity, thus suggesting the presence in planarians of a serotonin receptor of the type 5-HT<sub>1A</sub>.</p></div>","PeriodicalId":75683,"journal":{"name":"Cell biology international reports","volume":"16 5","pages":"Pages 427-432"},"PeriodicalIF":0.0,"publicationDate":"1992-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0309-1651(06)80062-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12557273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 17
Localization of chromatin in “persistent” nucleoli in okadaic acid treated hela cells 冈田酸处理的hela细胞中染色质在“持久”核仁中的定位
Cell biology international reports Pub Date : 1992-05-01 DOI: 10.1016/S0309-1651(06)80066-4
Sibdas Ghosh , Neidhard Paweletz
{"title":"Localization of chromatin in “persistent” nucleoli in okadaic acid treated hela cells","authors":"Sibdas Ghosh ,&nbsp;Neidhard Paweletz","doi":"10.1016/S0309-1651(06)80066-4","DOIUrl":"10.1016/S0309-1651(06)80066-4","url":null,"abstract":"<div><p>In okadaic acid treated HeLa cells, the chromosomes sometimes condense without being accompanied by nuclear envelope breakdown. These cells show “persistent” nucleoli. Within these “persistent” nucleoli the intranucleolar chromatin condenses and can be observed in the region of the dense nucleolar component (DNC) of the nucleoli. Other nucleolar components, namely the fibrillar centre (FC) and the granular component (GC) remain unchanged. These observations strongly speak for the localization of nucleolar chromatin (ribosomal cistrons) within the dense nucleolar component of the interphase nucleolus.</p></div>","PeriodicalId":75683,"journal":{"name":"Cell biology international reports","volume":"16 5","pages":"Pages 475-480"},"PeriodicalIF":0.0,"publicationDate":"1992-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0309-1651(06)80066-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12794746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
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