M.A. Muros, A.E. Aránega, C. Vélez, F.J. González, J.E. Fernández, L. Alvarez, A. Aránega
{"title":"胚胎心肌细胞原代培养过程中原肌球蛋白的变化","authors":"M.A. Muros, A.E. Aránega, C. Vélez, F.J. González, J.E. Fernández, L. Alvarez, A. Aránega","doi":"10.1016/S0309-1651(06)80064-0","DOIUrl":null,"url":null,"abstract":"<div><p>We chose the Hamburger and Hamilton's stage 29 (HH 29) to investigate the expression of tropomyosin in chick myocardiocytes during 14 days on culture. Throughout 14 days of cell culture, changes in cell morphology were accompained by a redistribution of tropomyosin in different cell compartments. We used FACScan, SDS-PAGE and densitometric analysis to quantify total cell tropomyosin and concentrations of this protein in different cell fractions. Tropomyosin was found mostly in the cytoskeletal fraction than in the cytoplasmic.</p><p>When we compared the densitometric values from SDS-PAGE of cells in different stages of development we found that in HH 19, tropomyosin was more abundant in the cytoplasmic than in the cytoskeletal fraction. By HH 29, the two fractions had become inverted, and in HH 39, tropomyosin was clearly more abundant in the cytoskeletal than in the cytoplasmic fraction.</p><p>In the IFI analysis, tropomyosin was found to label the Stress fiberlike structures (SFL) in different patterns depending on the area of the cell which expressed this protein.</p></div>","PeriodicalId":75683,"journal":{"name":"Cell biology international reports","volume":"16 5","pages":"Pages 451-464"},"PeriodicalIF":0.0000,"publicationDate":"1992-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0309-1651(06)80064-0","citationCount":"2","resultStr":"{\"title\":\"Changes in tropomyosin during primary culture of embryonic myocardiocytes\",\"authors\":\"M.A. Muros, A.E. Aránega, C. Vélez, F.J. González, J.E. Fernández, L. Alvarez, A. Aránega\",\"doi\":\"10.1016/S0309-1651(06)80064-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>We chose the Hamburger and Hamilton's stage 29 (HH 29) to investigate the expression of tropomyosin in chick myocardiocytes during 14 days on culture. Throughout 14 days of cell culture, changes in cell morphology were accompained by a redistribution of tropomyosin in different cell compartments. We used FACScan, SDS-PAGE and densitometric analysis to quantify total cell tropomyosin and concentrations of this protein in different cell fractions. Tropomyosin was found mostly in the cytoskeletal fraction than in the cytoplasmic.</p><p>When we compared the densitometric values from SDS-PAGE of cells in different stages of development we found that in HH 19, tropomyosin was more abundant in the cytoplasmic than in the cytoskeletal fraction. By HH 29, the two fractions had become inverted, and in HH 39, tropomyosin was clearly more abundant in the cytoskeletal than in the cytoplasmic fraction.</p><p>In the IFI analysis, tropomyosin was found to label the Stress fiberlike structures (SFL) in different patterns depending on the area of the cell which expressed this protein.</p></div>\",\"PeriodicalId\":75683,\"journal\":{\"name\":\"Cell biology international reports\",\"volume\":\"16 5\",\"pages\":\"Pages 451-464\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1992-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0309-1651(06)80064-0\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Cell biology international reports\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0309165106800640\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cell biology international reports","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0309165106800640","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Changes in tropomyosin during primary culture of embryonic myocardiocytes
We chose the Hamburger and Hamilton's stage 29 (HH 29) to investigate the expression of tropomyosin in chick myocardiocytes during 14 days on culture. Throughout 14 days of cell culture, changes in cell morphology were accompained by a redistribution of tropomyosin in different cell compartments. We used FACScan, SDS-PAGE and densitometric analysis to quantify total cell tropomyosin and concentrations of this protein in different cell fractions. Tropomyosin was found mostly in the cytoskeletal fraction than in the cytoplasmic.
When we compared the densitometric values from SDS-PAGE of cells in different stages of development we found that in HH 19, tropomyosin was more abundant in the cytoplasmic than in the cytoskeletal fraction. By HH 29, the two fractions had become inverted, and in HH 39, tropomyosin was clearly more abundant in the cytoskeletal than in the cytoplasmic fraction.
In the IFI analysis, tropomyosin was found to label the Stress fiberlike structures (SFL) in different patterns depending on the area of the cell which expressed this protein.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
