Beitrage zur Pathologie最新文献

{"title":"Ultrastructural and histochemical investigations of spindle-shaped corpuscles in phagocytic cells of lymph nodes","authors":"W. Roggendorf ,&nbsp;U. Gross","doi":"10.1016/S0005-8165(77)80082-6","DOIUrl":"10.1016/S0005-8165(77)80082-6","url":null,"abstract":"<div><p>In connection with various diseases, spindle-shaped or roundish 2-15 μm large corpuscles with a yellowish-green, partly brown color are found in phagocytic cells of lymph nodes of various localisation. There are three groups according to staining and ultrastructural characteristics.</p><ul><li><span>1.</span><span><p>Strongly Fe⁺⁺⁺ positive corpucles only,</p></span></li><li><span>2.</span><span><p>corpuscles with a positive PAS-reaction, Ziehl-Neelsen and Sudan black B stain and slight iron adsorption,</p></span></li><li><span>3.</span><span><p>particles with irregular stainability.</p></span></li></ul><p>Ultrastructurally, the spindle bodies of Group 1 have a uniform membrane, double in some sections and a narrow, finely granular rim, in which 50 ä large iron micelles are stored. The electrondense center contains structures which may correspond to remainders of cell organelles. The similarly shaped particles of Group 2 have a homogeneous, electron-dense center and sometimes half-moon-shaped, homogeneous, less dense appositions. A dense round center with finely honey-combed translucencies and marginal vacuoles characterizes the irregularly shaped, in part spindly corpuscles of Group 3. The energy-dispersion X-ray analysis indicates only iron for the particles of Group 1. A relation of the corpuscles to specific diseases, especially to sarcoidosis and certain therapeutic measures, is not evident.</p></div><div><p>Bei verschiedenen Grundkrankheiten finden sich in phagozytierenden Zellen von Lymphknoten unterschiedlicher Lokalisation spindelförmige oder rundliche 10 × 2 μm groβe Körperchen mit gelblich-grüner, teilweise brauner Eigenfärbe. Nach färberischem und ultrastrukturellem Verhalten gibt es 3 Gruppen.</p><ul><li><span>1.</span><span><p>Körperchen mit ausschlieβlich stark positivem Fe⁺⁺⁺-Nachweis,</p></span></li><li><span>2.</span><span><p>Körperchen mit positiver PAS-Reaktion, Färbung nach Ziehl-Neelsen, Sudanschwarz B und geringer Eisenadsorption,</p></span></li><li><span>3.</span><span><p>Partikel mit uneinheitlicher Färbbarkeit.</p></span></li></ul><p>Ultrastrukturell besitzen die Spindelkörper der 1. Gruppe eine abschnittsweise verdoppelte Einheitsmembran, einen schmalen feingranulären Randsaum, in dem 50 ä groβe Eisenmizellen eingelagert sind. Im elektronendichten, offenbar agglomerierten Zentrum sind vereinzelt Strukturen erkennbar, die Resten von Zellorganellen entsprechen können. Die ähnlich geformten Partikel der 2. Gruppe besitzen ein homogenes elektronendichtes Zentrum und gelegentlich halbmondförmige, weniger dichte, homogene Appositionen. Die unregelmäβig geformten, teilweise spindeligen Körperchen der 3. Gruppe kennzeichnet ein rundliches dichtes Zentrum mit feinwabigen Aufhellungen und randständigen Vakuolen. Die energiedispersive Röntgen-Mikroanalyse weist für die Partikel der 1. Gruppe ausschlieβlich Eisen nach. Eine bestimmte Beziehung der Körperchen zu Grundkrankheiten, insbesondere zu Sarkoid","PeriodicalId":75583,"journal":{"name":"Beitrage zur Pathologie","volume":"161 3","pages":"Pages 267-282"},"PeriodicalIF":0.0,"publicationDate":"1977-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0005-8165(77)80082-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11296924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of Stereologic Methodology to Inflammatory Infiltrates Residing in Oral Mucous Membrane Lesions Structural and Morphometric Comparison of Benign Mucous Membrane Pemphigoid and Reticular Lichen Planus","authors":"G.E. Petrzilka ,&nbsp;H.E. Schroeder","doi":"10.1016/S0005-8165(76)80031-5","DOIUrl":"10.1016/S0005-8165(76)80031-5","url":null,"abstract":"<div><p>In the present study, stereologic procedures are employed to estimate quantitatively the composition of chronic connective tissue infiltrates characterizing various oral mucous membrane diseases. One case each of benign mucous membrane pemphigoid (BMMP) and reticular lichen planus (RLP) served as examples to demonstrate the applicability of this methodology. In both lesions which are described clinically, histologically and on the ultrastructural level and which display a markedly different infiltrate composition, fibroblasts were affected and either proliferated (BMMP) or became cytopathologically altered (RLP). The quantitative data presented, besides providing new information, argue in favour of the stereologic approach.</p></div><div><p>In der vorliegenden Arbeit wird versucht, stereologische Methodik erstmals dazu zu verwenden, quantitativ die Zusammensetzung von chronischen Bindegewebsinfiltraten zu schätzen, welche verschiedenste Mundschleimhauterkrankungen charakterisieren. Um die Verwendbarkeit dieser Methodik zu demonstrieren, wurden je 1 Fall eines gutartigen Schleimhautpemphigoids (BMMP) und eines Lichen planus reticularis (RLP) klinisch, histologisch, ultrastrukturell und quantitativ morphometrisch untersucht. In beiden Läsionen, die eine sehr verschiedene Infiltratzusammensetzung aufwiesen, waren die ortsständigen Fibroblasten entweder proliferierend (BMMP) oder zytopathologisch (RLP) vom Infiltrat betroffen. Die quantitativen Daten, welche für beide Läsionstypen neue Information erbrachten, attestieren die Brauchbarkeit und den Wert einer stereologischen Analyse von chronisch entzündlichen Bindegewebsinfiltraten.</p></div>","PeriodicalId":75583,"journal":{"name":"Beitrage zur Pathologie","volume":"159 4","pages":"Pages 351-370"},"PeriodicalIF":0.0,"publicationDate":"1976-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0005-8165(76)80031-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11987823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electron Microscopic Study of Benign Hepatoma in a Patient on Oral Contraceptives","authors":"M. Balázs","doi":"10.1016/S0005-8165(76)80172-2","DOIUrl":"10.1016/S0005-8165(76)80172-2","url":null,"abstract":"<div><p>Electron microscopic studies of a benign hepatoma in a 31-year-old women who was on contraceptive pills are presented. The electron microscopic picture showed highly differentiated liver cells with regularly developed bile canaliculi. The mitochondrial polymorphism, the formation of paracristalloids and the appearance of giant mitochondria were striking. The main change was the occurence of the numerous capillaries of varying caliber which were highly differentiated and formed an integral part of the tumor. On the basis of these finding the tumor is regarded as a benign mixed tumor. The well developed capillary system explaines the arteriographic characteristics and the frequent bleeding complications noted in the literature.</p></div><div><p>Elektronenmikroskopische Untersuchungen eines gutartigen Hepatoms einer 31jährigen, mit oralen Antikonzeptiva behandelten Patientin werden durchgeführt. Die elektronenmikroskopischen Bilder zeigen reife Leberzellen mit regelmäßig entwickelten Gallenkanälchen. Der Polymorphismus der Mitochondria ist auffällig. Hauptbefunde sind: zahlreiche verschieden große Kapillaren, die gut entwickelt sind und einen fundamentalen Teil des Tumors bilden. Aufgrund dessen wird die Geschwulst für einen gutartigen Mischtumor gehalten. Das reichliche Kapillarsystem erklärt die arteriographische Charakteristik und die Blutungsneigung des Turmorgewebes.</p></div>","PeriodicalId":75583,"journal":{"name":"Beitrage zur Pathologie","volume":"159 3","pages":"Pages 299-306"},"PeriodicalIF":0.0,"publicationDate":"1976-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0005-8165(76)80172-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11406611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tubulusstruktur und Keimzellverteilung in frühkindlichen kryptorchen und normalen Hoden","authors":"H. Knecht","doi":"10.1016/S0005-8165(76)80168-0","DOIUrl":"10.1016/S0005-8165(76)80168-0","url":null,"abstract":"<div><h3>Introduction</h3><p>Many recent publications have demonstrated that the cryptorchid testicle (and, to a lesser extent, the descended partner) are progressively injured from the second year of life onwards. Do these injuries occur in an organ which has been healthy up to this time or are they superimposed on a structurally abnormal testicle? In order to answer this, parts of cryptorchid testicles, of the descended partners, and of normal testicles were compared by histological examination of serial sections.</p></div><div><h3>Material and Methods</h3><p>Parts of four testes from children aged 4–7 months (2 specimens obtained by biopsy and 2 from autoptic material) and parts of four testes from children 1½ years old (2 obtained by biopsy and 2 from autoptic material) were examined. The biopsies were fixed in Stieve's fixative. Tissue samples from clinically healthy children who had died suddenly were fixed in 4% formalin. The tissue was embedded in paraffin and sectioned serially; 6 μn sections were stained with HE.</p><p>The spermatogonia in each cross-section and in each oblique section of a same tubule were counted and the counts of the latter were adjusted to a cross-section 50–60 μn in diameter. This counting technique did not alter the density of spermatogonia. The graphs present data on the density of spermatogonia through the lengths of the tubules examined and demonstrate tubular branching and blind ends.</p><p>In the first year of life the cryptorchid testis and its descended partner showed repeated long sections lacking spermatogonia in the same tubule, whereas in normal testes the spermatogonia were more evenly distributed. The cryptorchid testis showed increased tubule branching in the areas examined.</p><p>In the second year of life the tubules of the cryptorchid testis and its descended partner manifest areas free of germ cells, increased branching, and blind ends. The cryptorchid testis also had a tubule completely free of spermatogonia. The germ cell-free parts were always associated with a smaller tubule diameter than normal. The normal testes did not disclose increased branching or spermatogonium-free areas within similar lengths of tubules and showed an even distribution of spermatogonia.</p></div><div><h3>Discussion</h3><p>The different distribution of spermatogonia within the tubules and the increased branching of the tubules in cryptorchid testes indicate a previous disturbance of testis development.</p></div><div><p>Anhand ausgesuchter Fälle wurden die Spermatogonienverteilung innerhalb längerer Tubulusabschnitte und der räumliche Aufbau tubulärer Strukturen bei kryptorchen Hoden, ihren deszendierten Partnern sowie bei Normalhoden in Serienschnitten vergleichend untersucht. Im ersten Lebensjahr finden sich Unterschiede in der Art der Spermatogonienverteilung und im Auftreten von tubulären Verzweigungen. Längere spermatogonienfreie Abschnitte im gleichen Tubulus wiederholen sich sowohl beim kryptorchen Hoden als auch","PeriodicalId":75583,"journal":{"name":"Beitrage zur Pathologie","volume":"159 3","pages":"Pages 249-270"},"PeriodicalIF":0.0,"publicationDate":"1976-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0005-8165(76)80168-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"55611883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[DNA Turnover in the Whole Body of Tumour Bearing Mice (author's transl)].","authors":"P H Kronenberger,&nbsp;W Porschen,&nbsp;L E Feinendegen","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Unlabelled: </strong>During the last 3 decades several authors have found in tumour-bearing animals an increase of synthesis and content of DNA in various organs which were free from neoplastic cells (Griffin, 1957;Kelly and Jones, 1950; Morgan and Cameron, 1973; Cerecedo et al., 1951; Lombardo et al., 1952). 5-iodo-2'-deoxyuridine (IUdR) is a thymidine analogue and specifically incorporated into DNA. When it is labelled with 125I or 131I it permits to reinvestigate these findings by measuring the rate of precursor incorporation into DNA and the rate of loss of labelled DNA in the living animal by means of counting the gamma emission from the incorporated iodine isotopes. In this paper, therefore, an attempt is made to analyse the DNA turnover in the whole body of liver tumour-bearing mice.</p><p><strong>Material and methods: </strong>5-iodo-2'-deoxyuridine (IUdR) labelled with 125iodine was used as DNA precursor. It is a thymidine analogue 5% of which is specifically incorporated into the DNA of those proliferating cells which are in the phase of DNA synthesis at the moment of tracer application. Non-incorporated IUdR (about 95% of the injected amount) is rapidly degraded and excreted within 24 hours. The tracer remains bound to the cellular DNA druing the life span of the labelled cells. After cell death only about 5% of IUdR from DNA breakdown is reutilized. 125I has a half live of 60 days and therefore allows, over periods of weeks, external measurements of the DNA turnover in the living animal without disturbing the physiological environment. The measured loss of DNA-bound 125I reflects almost exclusively the turnover of the labelled cells. Female albino NMRI mice, 2 months old, bearing sarcoma-180 implanted into the right hind leg were intraveneously injected with 2 muCi 125I-UdR. At the time of injection, the tumour had reached in one group of mice an average volume of about 25 mm3 and in another group an average volume of nearly 850 mm3. When implanted into subcutaneous tissue sarcoma-180 rarely produces metastases in parenchymal organs, never in the spleen and--within the first 30 days after implantation--only in ca. 10% of the animals a small metastasis in a single lymphnode (Deodhar and Crile, 1969; Franchi et al., 1968). Whole body measurements were carried out immediately after tracer injection and then daily during the first week and every second or third day in the following 2 weeks in a NaI well counter with a single channel pulse height analyser. The tumour activity was also determined in vivo by a special counting device.</p><p><strong>Results: </strong>In the normal mouse 4 to 6% of injected 125I-UdR is retained in the whole body 24 hours after tracer injection. During the following five days the 125I activity rapidly declines to 0.8% of that of day O immediately after injection. Thereafter the rate of loss of activity greatly diminishes (Fig. I). The first component of the turnover curve reflects an average daily ce","PeriodicalId":75583,"journal":{"name":"Beitrage zur Pathologie","volume":"159 4","pages":"398-412"},"PeriodicalIF":0.0,"publicationDate":"1976-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12202120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intestinal, Gastric Body- and Antral-Type Mucosal Metaplasia in the Gallbladder","authors":"M. Laitio","doi":"10.1016/S0005-8165(76)80169-2","DOIUrl":"10.1016/S0005-8165(76)80169-2","url":null,"abstract":"<div><p>The material consisted of two intestinal, one gastric body-type and 14 antral-type cases of mucosal metaplasia in the gallbladder.</p><p>Outside the mucosal areas mentioned, the epithelium of the gallbladder had undergone great changes. It usually consisted of goblet cells, enterochromaffin cells, superficial gastric-type epithelial islands and antral-type glands. Extensive areas contained non-sulphated acid mucin and neutral mucin. The areas of normal gallbladder epithelium were small.</p></div><div><p>Das Material bestand aus 17 Fällen von Schleimhautmetaplasien in der Gallenblase. In zwei Fällen war die Schleimhaut intestinalartig umgewandelt, in einem Fall wurden magenkörperartige Veränderungen beobachtet und 14 Fälle wiesen einen antralartigen Umbau der Schleimhaut auf.</p><p>Über die Änderungen in der Gallenblasenschleimhaut hinaus wurden auch im Gallenblasenepithel erhebliche Veränderungen beobachtet. Es handelte sich meistens um Becherzellen, enterochromaffine Zellen, Epithelinseln vom Typ des Oberflächenepithels im Magenkörper sowie Drüsen, die denen des Antralbereiches glichen. In umfangreichen Teilen der Gallenblase konnten nonsulphiertes saures Muzin sowie neutrales Muzin nachgewiesen werden. Intaktes Gallenblasenepithel war nur noch wenig erhalten.</p></div>","PeriodicalId":75583,"journal":{"name":"Beitrage zur Pathologie","volume":"159 3","pages":"Pages 271-279"},"PeriodicalIF":0.0,"publicationDate":"1976-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0005-8165(76)80169-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11357577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative Electron Microscopic Study of Capillaries in Diabetes mellitus","authors":"M. Ravid ,&nbsp;R. Silman-Socher,&nbsp;Y. Ben Shaul,&nbsp;E. Sohar","doi":"10.1016/S0005-8165(76)80170-9","DOIUrl":"10.1016/S0005-8165(76)80170-9","url":null,"abstract":"<div><p>A systematic microscopic examination of all elements of the capillary wall was performed on quadriceps muscle biopsies from 9 diabetic patients and 8 controls.</p><p>The capillary basement membrane (CBM) was markedly thicker in diabetics; it consisted of several lamellae and contained large vacuoles which were never observed in non-diabetic subjects. Large magnifications revealed fibrils in greater number and markedly larger in diameter in diabetics, these accounting for a considerable volume of the CBM and the adventitia and increased diameter and thickness of the capillary wall, without encroaching on the lumen. The intracellular fibrils in pericytes and endothelial cells were also larger and thicker in diabetic subjects.</p><p>The prevalence of fibrillar material in the vascular disease of diabetes mellitus suggests the importance of research into possible measures to arrest fibril formation.</p></div><div><p>Quadriceps-Muskel-Biopsien von 9 diabetischen und 8 nicht-diabetischen Individuen wurden elektronenmikroskopisch untersucht.</p><p>Die Basalmembran der Kapillaren bei Diabetikern war deutlich dicker und bestand aus einigen Lamellen. Sie enthielt große Vakuolen, welche bei den Nicht-Diabetikern fehlten.</p><p>Bei starker Vergrößerung wurden in der Basalmembran bei den Diabetikern zahlreiche dicke Fibrillen gefunden. Diese Fibrillen nahmen einen beträchtlichen Teil des Volumens der kapillaren Basalmembran und der Adventitia ein. Der Durchmesser und die Dicke der Kapillarenwand war vergrößert, ohne jedoch das Kapillarlumen einzuengen.</p><p>Die intrazellulären Fibrillen in den Perizyten und Endothelzellen der Diabetiker waren ebenfalls dicker.</p></div>","PeriodicalId":75583,"journal":{"name":"Beitrage zur Pathologie","volume":"159 3","pages":"Pages 280-291"},"PeriodicalIF":0.0,"publicationDate":"1976-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0005-8165(76)80170-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12195679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Postmortem demonstration of portal vein collateral circulation (author's transl)].","authors":"G Brandt,&nbsp;M Stolte","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Introduction: </strong>Postmortem investigation of esophageal varices and the portal system is difficult, because veins are collapsed at autopsy. The demonstration of varices is possible by illumination of the isolated mucous membrane or by puncturing esophageal varices and filing them with different materials.</p><p><strong>Material and methods: </strong>We use a 15% aqueous gelatine solution (if needed with additional barium sulfate for x-ray examination) which is supplemented by 40% formaldehyde (40 ml in 1 1 gelatine solution). The superior mesenteric vein is catheterized and filled by a clyster-pump.</p><p><strong>Results: </strong>The mixture of gelatine and formaldehyde hardens within a few minutes. The autopsy is delayed only about half an hour. Within this time the portal system is well outlined. This method can show exactly the drainage of the portal system into the inferior vena cava. In cases of portocaval shunt or of esophageal transsection the result of the operation can be verified. The localization of the bleeding source of esophageal varices can be demonstrated by escape of the filling mass.</p>","PeriodicalId":75583,"journal":{"name":"Beitrage zur Pathologie","volume":"159 3","pages":"307-13"},"PeriodicalIF":0.0,"publicationDate":"1976-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12195683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Classification of Tumor Development Based on an Analysis of Enzymes in Tissue Sections of Hypernephroid Carcinoma in Man","authors":"E.D. Wachsmuth ,&nbsp;J.P. Stoye","doi":"10.1016/S0005-8165(76)80167-9","DOIUrl":"10.1016/S0005-8165(76)80167-9","url":null,"abstract":"<div><p>Sections of hypernephroid carcinoma from 20 cases were investigated for aldolase isozymes A and B by a mixed aggregation immuno-cytochemical technique, and for the brush border membrane enzymes aminopeptidase and alkaline phosphatase by conventional histochemical techniques. It was found that the cases could be grouped into four types: type 1 (1 case) contained all 4 enzymes; type 2 (7 cases) contained all enzymes except aldolase-B; type 3 (7 cases) possessed aldolase-A and one brush border membrane enzyme; type 4 (5 cases) contained only aldolase-A. The aldolase-A concentration in all tumor cells was higher than that in proximal tubule cells, whereas the concentration of the two brush border enzymes was lower. In cases type 2 and 3, but not in type 4 occasional patches of cells were found to contain some aldolase-B and/or higher amounts of the brush border enzymes than the surrounding cells. No correlation was observed between clear cell and granular cell hypernephroid carcinomas or the invasiveness or the nuclear polymorphism of the tumors on the one hand with their enzyme type on the other.</p><p>These histological enzyme analyses suggest that most, if not all, hypernephroid carcinomas are derived from kidney proximal tubule cells and that the tumor cells then progressively lose aldolase-B, and subsequently the brush border enzymes, but at the same time producing more aldolase-A. The presence of the enzyme-rich patches suggest different patterns of proliferation and differentiation among the tumor cell population.</p><p>Three tumors other than hypernephroid carcinoma were also examined in this way. The results suggest that histoenzymological analyses are of general applicability in studies of tumor progression. They should also be useful for biopsy and aspiration cytology.</p></div><div><p>Hypernephroide Karzinome von 20 Patienten wurden nach operativer Exstirpation auf ihren Gehalt an Aldolase-A, Aldolase-B und den Bürstensaumenzymen, Aminopeptidase und alkalische Phosphatase, in Gewebehomogenaten und am Gewebeschnitt untersucht. Eine immunozytochemische Methode, beruhend auf dem immunochemischen Prinzip einer gemischten Aggregation, diente dem Nachweis und der Lokalisation der Aldolasen, übliche histochemische Methoden dem Nachweis der beiden Bürstensaummembranenzyme Aminopeptidase und alkalische Phosphatase. Die Ergebnisse von Homogenatanalysen waren für eine Klassifizierung der Progredienz des Karzinoms nicht brauchbar im Gegensatz zur Gewebsschnittanalyse. Letztere erlaubte eine Einteilung der Karzinome in 4 Typen: Typ 1 (1 Fall) besaß alle 4 Enzyme, Typ 2 (7 Fälle) enthielt alle Enzyme außer Aldolase-B, Typ 3 (7 Fälle) besaß Aldolase-A und eines der Bürstensaummembranenzyme, Typ 4 (5 Fälle) enthielt nur Aldolase-A, keines der anderen 3 Enzyme war nachweisbar. Die Konzentrationen an Aldolase-A waren in allen Tumorzellen höher, die der Bürstensaummembranenzyme und der Aldolase-B niedriger als in den Hauptstücken des Nephrons. In de","PeriodicalId":75583,"journal":{"name":"Beitrage zur Pathologie","volume":"159 3","pages":"Pages 229-248"},"PeriodicalIF":0.0,"publicationDate":"1976-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0005-8165(76)80167-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12197098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Lymph Diversion Versus Corticosteroids on Blood and Lymphocyte Pools","authors":"H. Buerki ,&nbsp;B. Massner ,&nbsp;H. Cottier ,&nbsp;A.D. Chanana ,&nbsp;D.D. Joel ,&nbsp;E.P. Cronkite","doi":"10.1016/S0005-8165(76)80030-3","DOIUrl":"10.1016/S0005-8165(76)80030-3","url":null,"abstract":"<div><p>Thoracic duct-rumen fistulae (TDRF) and low or high doses of Prednisolone were employed to induce depletion of blood and lymph node lymphocytes in calves. Elimination of the easily mobilizable pool of lymphocytes was almost complete within 6 days after commencing TDRF. Blood lymphocyte counts were reduced to approximately ⅓ of pre-TDRF levels. Prednisolone in low doses did not decrease the number of blood lymphocytes. High doses of Prednisolone induced a moderate, progressive blood lymphocytopenia. Both TDRF and low and high dose Prednisolone schedules induced a significant reduction of lymphocytes in lymph nodes. The loss of small lymphocytes from lymph nodes was almost entirely restricted to the diffuse cortical (paracortical) zone. The lymphocyte counts in the follicular cortex and in the medulla were not significantly affected. Almost complete disappearance of germinal centers was observed in lymph nodes of calves subjected to TDRF or high doses of Prednisolone.</p></div><div><p>Im Zuge vergleichender Untersuchungen über experimentelle Lymphopenie wurden Kälbern entweder eine Fistel zwischen Ductus thoracicus und Magen (TDRF, thoracic duct-rumen fistula) angelegt oder Prednisolon in niedriger und hoher Dosierung verabreicht. Innerhalb der ersten 6 Tage nach Herstellung der TDRF war fast der ganze leicht mobilisierbare Teil der Lymphozyten ausgeschaltet, und die Blutlymphozytenwerte fielen auf ungefähr ein Drittel der Ausgangswerte. Prednisolon in niedriger Dosierung beeinflußte die Zahl der Blutlymphozyten kaum, bewirkte indessen in hoher Dosis eine mäßige progressive Lymphozytopenie. In den Lymphknoten wurde die Gesamtzahl der Lymphozyten sowohl durch TDRF als auch durch niedrige und hohe Dosen von Prednisolon signifikant vermindert. Dieser Lymphozytenverlust in den Lymphknoten beschränkte sich fast ausschließlich auf die tiefe kortikale (parakortikale) Zone, die überwiegend T-Zellen enthält. Die von mehr oder weniger seßhaften B-Lymphozyten besiedelten Primärfollikel, die lymphozytäre Mantelzone der Sekundärfollikel und die Medulla blieben fast unverändert, während die Keimzentren durch Anlegen einer TDRF oder durch hohe Dosen von Prednisolon weitgehend zum Verschwinden gebracht wurden.</p></div>","PeriodicalId":75583,"journal":{"name":"Beitrage zur Pathologie","volume":"159 4","pages":"Pages 343-350"},"PeriodicalIF":0.0,"publicationDate":"1976-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0005-8165(76)80030-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12202119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}