Archives roumaines de pathologie experimentales et de microbiologie最新文献

{"title":"Etiologic studies on the association of goat pox with peste des petits ruminants (PPR) disease in Nigeria.","authors":"L O Wosu, C N Chineme","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75543,"journal":{"name":"Archives roumaines de pathologie experimentales et de microbiologie","volume":"48 1","pages":"79-83"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13697014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative profile of cardiolipin and group treponemal IgD antibodies in syphilis estimated by single radial immunodiffusion technique (SRID).","authors":"A D Ionescu,&nbsp;M Petcovici,&nbsp;T Ionescu-Dorohoi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>150 serum samples (reactive in VDRL, Reiter-ELISA, FTA-Abs tests), from male patients 25-45 years old, in various stages of syphilis whether treated or untreated, were tested for IgD by SRID. On 154 sera from healthy males 25-45 years old, the reference normal values for IgD levels were established, as: 0-131.2 IU/ml with a mean of 29.92 +/- 29.61 IU/ml. Cardiolipin and group treponemal fraction values for IgD class were obtained by assessing the difference between the immunodiffusion diameter values produced by sera before and after complete absorption with VDRL antigen or delipidated T. reiteri suspension. The individual, mean +/- SD values (expressed in IU/ml) and the percentage of cardiolipin and treponemal IgD of the total IgD class were calculated for each stage. The mean value of the total IgD class, excepting secondary syphilis (sigma 2) 52.53 +/- 26.66 IU/ml), did not overstep the normal levels but all minimal individual values from syphilitic patients (7.09-14.89 IU/ml) surpassed significantly the normal minimal values which were less than or equal to 3.54 IU/ml. The total lack of cardiolipin (IgD and the presence of group treponemal IgD in all sera of the syphilis stages studied were manifest. The group treponemal IgD mean values ranged between 7-9 IU/ml, with a maximum of 19.32 +/- 10.58 IU/ml in sigma 2 followed by latent syphilis (sigma lat) with a mean value of 9.37 +/- 4.9 IU/ml. A significant percentage of treponemal IgD vs total IgD was recorded: primary syphilis (sigma 1) 32.01%, primary-secondary syphilis (sigma 1-2) 28.76%, sigma 2 36.77%, sigma lat and treated persistent seroreactive syphilis (sigma t+) 29.61%. The high proportion of treponemal IgD in latent and treated persistent reactive syphilis suggests a steady activation of B lymphocytes by treponemal antigens and presumably is an expression of an active infectious process. The absence of cardiolipin IgD and the presence of only the treponemal IgD, in all sera from all stages, might confer to their detection an extremely specific diagnostic value in syphilis.</p>","PeriodicalId":75543,"journal":{"name":"Archives roumaines de pathologie experimentales et de microbiologie","volume":"48 1","pages":"19-31"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13652057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative evaluation of three in vacuo dessication procedures on bacterial cultures.","authors":"E Burlacu,&nbsp;C David,&nbsp;T Vasilescu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The paper presents results obtained for the bacterial cultures preservation (E. coli ATCC 25 922 and S. aureus Wood) by three in vacuo desiccation procedures: freeze-drying (lyophilization or cryo-desiccation at eutectic zone), cryo-desiccation above eutectic zone and direct drying. It has been in view: the survival of liquid cultures as reported to the desiccation procedure per se, the loss of viability of desiccated cultures stored in refrigerator for at least one year and the residual moisture of desiccated cultures. For 3 batches there has been applied the accelerated thermal degradation test. Employing the same protective medium for both cultures, E. coli cultures prove to be more easily affected by lyophilization and cryo-desiccation above eutectic zone as compared to S. aureus cultures, fact that may be due to the differences between the wall structures of G--bacteria and G+ bacteria, respectively. During storage, E. coli and S. aureus cultures proved a quite similar loss of viability. The residual moisture content was quite similar for both E. coli and S. aureus cultures exposed to the same in vacuo desiccation procedure. The lyophilization and the cryo-desiccation above eutectic zone, as compared to direct drying, yielded superior results. The accelerated thermal degradation test provides only informative results, partially confirmed by viable counts determined at stated intervals of storage time in the refrigerator.</p>","PeriodicalId":75543,"journal":{"name":"Archives roumaines de pathologie experimentales et de microbiologie","volume":"48 1","pages":"65-78"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13820380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An experimental model comparing the antineoplastic and the immunosuppressive effects of some cytostatics.","authors":"G Ghyka,&nbsp;L Haraga","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>An experimental model evaluating comparatively the antineoplastic and the immunosuppressive effects of some cytostatics (cyclophosphamide and vinblastine) is described in the present paper. Different cytostatic doses were intraperitoneally administered in mice which were 24 hours later grafted with a suspension of human tumoral KB cells. The xenograft acceptance was macroscopically and microscopically recorded 21 days later. By the Reed and Muench method and also by graphical extrapolation the LD50 (lethal dose for 50% animals) and the TD50 (the immunosuppressive dose enabling 50% animals to accept the xenografts) could thus be determined for both cyclophosphamide and vinblastine. The number (percent) of the tumour bearing animals three weeks after grafting was considered as an indicator of the cytostatic dose at which the immunosuppressive effect exceeded the antineoplastic effect. The in vitro effect of the same drugs on the KB cells was tested by inoculating different cytostatic doses in the cell cultures and counting at different time intervals the adherent as well as the nonadherent cells. The in vitro drug toxicity on the KB cell cultures was also determined by the trypan blue exclusion test. Both cyclophosphamide and vinblastine proved to be in vitro highly potent cytostatics i.e. when directly acting on the KB cells. This effect was dose correlated for both the considered drugs. However our in vivo experiments have shown that none of the observed effects when considering the direct action of the cytostatic on the cultured cells could not safely be extrapolated in vivo. Our results have an obvious practical importance when considering the therapeutical approaches in the neoplastic diseases. They demonstrate that the increase in cytostatic dose is not directly correlated to the antineoplastic effect since it reaches a limit at which the immunosuppressive effect highly exceed the tumour growth inhibition effect. The described experimental model could also be used in comparative estimations of the biological effects of different cytostatic drugs possibly referred to a standard immunosuppressive reagent as an antilymphocyte antiserum.</p>","PeriodicalId":75543,"journal":{"name":"Archives roumaines de pathologie experimentales et de microbiologie","volume":"48 1","pages":"33-45"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13942272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Q fever urban cases in Romania.","authors":"E Crăcea,&nbsp;S Constantinescu,&nbsp;N Tofan,&nbsp;F Căruntu,&nbsp;D Dogaru","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Q fever urban sporadic cases in Romania in the 1981-87 period are reviewed as concerns their incidence, seasonality and epidemiological data. Urban cases represented 76.8% as against 23.2% rural cases from the 134 Q fever sporadic cases detected in this period. Cases were distributed throughout all months with peaks during April (18.4% of cases) and June (19.4%). Infections were not related to contact with livestock or domestic birds or with raw milk drinking. Cases could not be identified as Q fever occupational ones. 36.7% of patients were working in nonalimentary industry and only 12.6% were involved in meat industry, veterinary or human medical practices. New studies concerning unusual sources of urban infection such as dogs, cats or urban street pigeons are emphasized.</p>","PeriodicalId":75543,"journal":{"name":"Archives roumaines de pathologie experimentales et de microbiologie","volume":"48 1","pages":"13-7"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13942270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The presence and significance of some anti-enzyme antibodies (anti-plasminogen, anti-trypsin, anti-phospholipase C) in rheumatoid arthritis (RA) and reactive arthritis (rA).","authors":"M Stefănescu,&nbsp;G Szegli,&nbsp;L Cremer,&nbsp;L Zarma,&nbsp;E Mazilu,&nbsp;M Naghiu,&nbsp;D Niculescu,&nbsp;A Gacheş,&nbsp;A Onu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>It is a well known fact that during the course of RA and rA, due to some cellular and molecular mechanisms, a number of proteolytic enzymes and membranal phospholipases are either activated or their concentration rises significantly. With this aim in view in the present work we have aimed to investigate if, in an inflammatory process associated to RA and rA, the human organism produces or not anti-enzyme antibodies. Using an enzyme-linked immunosorbent assay (ELISA), in the sera of 19 patients of RA and 19 of rA, the presence of the following antibodies has been ascertained: antiplasminogen (antiPAb), antitrypsin (antiTAb) and antiphospholipase C (antiPLCAb). Out of RA cases, 47.3% presented antiPAb, 36.8% antiTAb and 26.3% antiPLCAb. As it was expected, in the rA cases, these antibodies were found in a higher proportion of cases, i.e.: 85.7%, 71.4% and 57.1% respectively. When following the same cases before and during or after the treatment with nonsteroidal antiinflammatory agents (NSAIAs), the antibodies levels were significantly decreased especially in the RA cases. The results obtained indicated a significant rise of seric concentration of antiPAb, antiTAb and antiPLCAb, as well in RA as in rA. While the rise of antiPLCAb in RA and especially in rA could be explained due to microbial infections, the rise of the antiproteolytic enzymes antibody levels as well as the decrease of the antibody titres during or after NSAIAs treatment could be explained, in our opinion, on the basis of some autoimmune processes.</p>","PeriodicalId":75543,"journal":{"name":"Archives roumaines de pathologie experimentales et de microbiologie","volume":"48 1","pages":"47-53"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13942274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative profile of cardiolipin and group treponemal IgG, IgA and IgM antibodies in syphilis assayed by radial immunodiffusion technique.","authors":"A D Ionescu,&nbsp;M Petcovici,&nbsp;T Ionescu-Dorohoi","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75543,"journal":{"name":"Archives roumaines de pathologie experimentales et de microbiologie","volume":"47 3-4","pages":"261-82"},"PeriodicalIF":0.0,"publicationDate":"1988-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14281271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Follow-up cell-mediated immune functions in \"professional\" blood donors.","authors":"A Olinescu,&nbsp;S Hristescu,&nbsp;G Manda,&nbsp;M Neagu,&nbsp;T Georgescu","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75543,"journal":{"name":"Archives roumaines de pathologie experimentales et de microbiologie","volume":"47 3-4","pages":"283-8"},"PeriodicalIF":0.0,"publicationDate":"1988-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14395245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibitory effect of interleukin-1 on the in vitro PHA-stimulated lymphocytes of some blood donors.","authors":"A Olinescu,&nbsp;S Hristescu,&nbsp;G Manda,&nbsp;F Agache,&nbsp;M Neagu","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75543,"journal":{"name":"Archives roumaines de pathologie experimentales et de microbiologie","volume":"47 3-4","pages":"289-97"},"PeriodicalIF":0.0,"publicationDate":"1988-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14395247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of antimicrobial agents on Pseudomonas aeruginosa strains isolated from sporadical cases and nosocomial infections.","authors":"E Meitert,&nbsp;F Sima,&nbsp;C Savulian,&nbsp;E Chersulick,&nbsp;A M Petrescu","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75543,"journal":{"name":"Archives roumaines de pathologie experimentales et de microbiologie","volume":"47 3-4","pages":"227-38"},"PeriodicalIF":0.0,"publicationDate":"1988-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14281270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}