I Ioniţă, F Căruntu, M Dragomirescu, D Petraşincu, O Dorobăţ, A Streinu-Cercel, C Antipa
{"title":"Interferon detection in patients with neuroinfections: possibilities of clinical use.","authors":"I Ioniţă, F Căruntu, M Dragomirescu, D Petraşincu, O Dorobăţ, A Streinu-Cercel, C Antipa","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75543,"journal":{"name":"Archives roumaines de pathologie experimentales et de microbiologie","volume":"49 2","pages":"177-82"},"PeriodicalIF":0.0,"publicationDate":"1990-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12877341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V F Dima, I N Mihăilescu, S V Dima, L Chivu, M Stirbeţ, M Udrea, A Popa
{"title":"Studies of the effects of associated photodynamic therapy and drugs on macromolecular synthesis of tumoral cells grown in vitro.","authors":"V F Dima, I N Mihăilescu, S V Dima, L Chivu, M Stirbeţ, M Udrea, A Popa","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>HeLa S3 tumoral cells were used as an experimental model for studying the association of photodynamic therapy (PDT) and antitumoral agents. Tumoral monolayer cultures were incubated 18 hours at 37 degrees C with Photofrin II, trypsinized and suspended in Eagle medium supplemented with 10% FCS and then treated with antitumoral agents 90 minutes before He-Ne laser exposure. The tumoral cells were exposed to antitumoral agents in the following concentrations (equivalent to ED70): adriamycin (0.0297 micrograms); mitomycin C (0.0199 micrograms); 5-FU (0.4937 micrograms) and vinblastine (0.0109 micrograms) per 10(5) cells. Macromolecular syntheses (DNA, RNA and proteins) were investigated by use of radioactive precursors: 3H-thymidine, 3H-uridine and 3H-leucine, as expressed in percent referring to Photofrin II-pretreated controls; they were exposed to He-Ne laser but not treated with antitumoral agents. All experiments were followed for 72 hours incubation at 37 degrees C. The conclusions of the results of PDT associated with antitumoral agents sustain the following aspects: a) the antitumoral agents activity (adriamycin, mitomycin C, 5-FU, vinblastine) was more noticeable when applied 90 minutes before He-Ne laser irradiation; b) inhibition of radioactive precursors uptake in DNA, RNA and proteins was accompanied by suppression of in vitro tumoral cells development and c) PDT association with antitumoral agents could manifest at least three positive effects upon animals; 1) PDT potentiating effects with antitumoral agents; 2) suppressing effects on tumoral macromolecular synthesis; 3) antitumoral agents cytotoxic elimination (due to the low doses used).</p>","PeriodicalId":75543,"journal":{"name":"Archives roumaines de pathologie experimentales et de microbiologie","volume":"49 2","pages":"155-75"},"PeriodicalIF":0.0,"publicationDate":"1990-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13283865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Serotyping of Pseudomonas aeruginosa strains by slide coagglutination.","authors":"E Meitert, A M Petrescu, A Butoianu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Serological typing of Pseudomonas aeruginosa strains (228 strains) by slide coagglutination, using our own reagents (5 polyvalent and 22 monovalent ones, corresponding to the 22 serotypes in Meitert-Meitert scheme), led to identical results obtained by conventional slide agglutination. Utilization of live Ps. aeruginosa cells suspensions, killed by boiling or autoclaving, showed a 100% concordance of results, when using the second and the third suspension types and a 97.37% one between them and the live cells suspension. We noticed that reactions intensity was higher when using bacterial suspensions, boiled for 2.5 hours, in comparison with autoclaved cells suspensions, 30 minutes at 120 C. Compared to conventional slide agglutination, the slide coagglutination presents more advantages, being simple, rapid, specific and economical.</p>","PeriodicalId":75543,"journal":{"name":"Archives roumaines de pathologie experimentales et de microbiologie","volume":"49 1","pages":"37-42"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13283862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Seroepidemiological evidence of widespread enteroviral infection in Anambra State, Nigeria.","authors":"C U Iroegbu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Serum samples from 339 apparently healthy subjects, 298 adult blood donors and 41 infants aged 21 months and below, were screened for neutralising activity against poliovirus 1 and Coxsackieviruses A7 and B4. Among the adult serum samples 85% showed high activity (greater than or equal to 320) against poliovirus 1 while frequencies of similar activities were 65% and 64% for Coxsackieviruses A7 and B4 respectively. About 60% showed multiple activity against all three viruses; 15% against both Coxsackieviruses A7 and B4, and less than 5% for Coxsackievirus A7 and poliovirus 1, and Coxsackievirus B4 and poliovirus 1, respectively. Similar activities were detected in the sera of the infants. These show that enterovirus infections are endemic, multiple infections being the rule.</p>","PeriodicalId":75543,"journal":{"name":"Archives roumaines de pathologie experimentales et de microbiologie","volume":"49 1","pages":"63-9"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13125481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Standardised reproducible hemagglutination test technique for simple veterinary clinics in developing countries.","authors":"L O Wosu, U T Ugochukwu","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75543,"journal":{"name":"Archives roumaines de pathologie experimentales et de microbiologie","volume":"49 1","pages":"93-100"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13256262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G Dimache, C Stoean, S Durbacă, M Croitoru, M Ionescu, I N Nedelcu, I Corbu
{"title":"Study of specific immune response to unadsorbed concentrated tetanus vaccine administered by intradermal route to non-immunized persons in the last ten years.","authors":"G Dimache, C Stoean, S Durbacă, M Croitoru, M Ionescu, I N Nedelcu, I Corbu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Investigations of anti-tetanus response, in 404 subjects, most of them aged 60, being non-immunized for at least 10 years, stressed out the fact that 28.7% were not protected and 6.18% presented a protecting titer of 0.01 IU/ml, evaluated by \"in vivo\" protection test in mice. Some subjects were immunized with unadsorbed Tetanus vaccine (10 Lf/0.1 ml/dose) by i.d. route, using Jet-injector, and the others with adsorbed Tetanus vaccine (0.5 ml/dose), by i.m. route, using the needle and syringe. The vaccines were well tolerated and adverse reactions were not recorded. After 30 days, a single vaccine dose produced a protecting effect in 97.45% of non-protected subjects, belonging to i.d. immunized group, and also in 93.33% belonging to i.m. immunized group. 30 days after the administration of a second dose, protection set up in all subjects, no matter of vaccine type and administration route used. For a continuous reduction of tetanus morbidity, the authors suggest a specific periodical immunization of non-protected persons, selected by serological screening, using unadsorbed Tetanus vaccine, administered by i.d. route by means of the Jet-injector.</p>","PeriodicalId":75543,"journal":{"name":"Archives roumaines de pathologie experimentales et de microbiologie","volume":"49 1","pages":"51-62"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13256309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M Dumitrescu, D Panaitescu, M Hillebrand, E Volanschi, A Bălan
{"title":"Spectrophotometric assay of the interaction between malaria erythrocyte lysates with methylene blue and neutral red dyes.","authors":"M Dumitrescu, D Panaitescu, M Hillebrand, E Volanschi, A Bălan","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Changes of oxidative processes induced in mouse erythrocytes by Plasmodium berghei were studied in the presence of methylene blue, neutral red or of both cationic redox dyes. The results are discussed in terms of redox and metachromatic modifications of the dyes which are produced by malarial and normal erythrocyte lysates.</p>","PeriodicalId":75543,"journal":{"name":"Archives roumaines de pathologie experimentales et de microbiologie","volume":"49 1","pages":"71-8"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13256269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Nuclear bodies and virus-like particles in gingival tissue of periodontopathic patients.","authors":"B Burghelea, H Serb","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75543,"journal":{"name":"Archives roumaines de pathologie experimentales et de microbiologie","volume":"49 1","pages":"89-92"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13256830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E Neguţ, R Arion, I Peligrad, L Dincă, C Matache, I Dinu, G Dinu, Z Langa, G Szegli
{"title":"Biological preparations with clinical efficacy in the prevention and therapy of allergic syndromes.","authors":"E Neguţ, R Arion, I Peligrad, L Dincă, C Matache, I Dinu, G Dinu, Z Langa, G Szegli","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75543,"journal":{"name":"Archives roumaines de pathologie experimentales et de microbiologie","volume":"49 1","pages":"17-30"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13256306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Bacterial agents associated with bronchopulmonary disorders in eastern Nigeria.","authors":"C U Iroegbu, A N Njoku-Obi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Altogether 16,539 sputum specimens were examined microbiologically from 1980 to 1984. Out of these 12,588 were screened by Ziehl-Neelsen's staining technique and 782 were (6.3%) found AFB-positive. Age and sex distributions of the AFB-positive individuals were statistically significant (at 0.05), incidence being most prevalent among those 20 years and above (90.2%) and among males (61.2%). From other specimens cultured, non-AFB organisms were isolated at the following frequencies: coliform-like organisms (15.1%), Streptococcus pneumoniae (55.5%). Klebsiella pneumoniae (5.3%), Streptococcus pyogenes (3.9%), Pseudomonas aeruginosa (3.4%), Haemophilus influenzae (3.0%). Proteus Spp. (0.7%) and Escherichia coli (0.5%). The antibiogram of these isolates revealed a high incidence of multiple antibiotic resistance, a situation that has most probably arisen from the high degree of antibiotic misuse in Nigeria.</p>","PeriodicalId":75543,"journal":{"name":"Archives roumaines de pathologie experimentales et de microbiologie","volume":"49 1","pages":"43-50"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13256307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}