Archives de l'Institut Pasteur de Tunis最新文献_第5页

[Avian infectious bronchitis disease in Tunisia: seroprevalence, pathogenicity and compatibility studies of vaccine-field isolates]. [突尼斯的禽传染性支气管炎:疫苗场分离株的血清流行率、致病性和相容性研究]。

Archives de l'Institut Pasteur de Tunis Pub Date : 2009-01-01

H Bourogaa, K Miled, I Larbi, J Nsiri, L Gribaa, I El Behi, W Ben Rhouma, E Allagui, H Sassi, A Ghram

{"title":"[Avian infectious bronchitis disease in Tunisia: seroprevalence, pathogenicity and compatibility studies of vaccine-field isolates].","authors":"H Bourogaa, K Miled, I Larbi, J Nsiri, L Gribaa, I El Behi, W Ben Rhouma, E Allagui, H Sassi, A Ghram","doi":"","DOIUrl":"","url":null,"abstract":"A sero-epidemiological study was carried out on 5660 sera collected, between 2006 and 2008, from different flocks in different regions of the country. The ELISA results showed low levels of antibodies indicating vaccination failures. 45 to 69% of the flocks showed positive levels of antibodies and only 5 to 15% of these were protected. The pathogenicity studies of the Tunisian field isolates TN20/00 and TN335/01 demonstrated high clinical and lesion scores indicating the pathogenic effect of the two isolates. The challenge experiments conducted to evaluate the cross-protection between the H120 vaccine and the field isolates showed low protection rate, especially against the TN20/00 virus. The overall results allowed the determination of the pathogenic nature of the field isolates and a vaccination program based on the use of the only Massachusetts H120 strain did not reduce tracheal and kidney lesions. To better control the disease, adapting the vaccination program by using vaccine allowing better protection against variant strains, is recommended.","PeriodicalId":75537,"journal":{"name":"Archives de l'Institut Pasteur de Tunis","volume":"86 1-4","pages":"75-83"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29186588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Immuno-modulating effects of eukaryotic expressing vectors of IL-12 and GM-CSF associated to DNA-based vaccination against experimental cutaneous leishmaniasis in BALB/c mouse]. [IL-12和GM-CSF真核表达载体对BALB/c小鼠实验性皮肤利什曼病的免疫调节作用]。

Archives de l'Institut Pasteur de Tunis Pub Date : 2009-01-01

S Ben Hadj Ahmed, K Dellagi, C Bahloul

引用次数: 0

Functional polymorphisms of PTPN22 and FcgR genes in Tunisian patients with rheumatoid arthritis. 突尼斯类风湿性关节炎患者PTPN22和FcgR基因的功能多态性

Archives de l'Institut Pasteur de Tunis Pub Date : 2009-01-01

I Sfar, T Dhaouadi, I Habibi, L Abdelmoula, M Makhlouf, T Ben Romdhane, S Jendoubi-Ayed, H Aouadi, T Ben Abdallah, K Ayed, R Zouari, Y Lakhoua-Gorgi

{"title":"Functional polymorphisms of PTPN22 and FcgR genes in Tunisian patients with rheumatoid arthritis.","authors":"I Sfar, T Dhaouadi, I Habibi, L Abdelmoula, M Makhlouf, T Ben Romdhane, S Jendoubi-Ayed, H Aouadi, T Ben Abdallah, K Ayed, R Zouari, Y Lakhoua-Gorgi","doi":"","DOIUrl":"","url":null,"abstract":"To investigate a possible association between functional polymorphisms of the protein tyrosine phosphatase nonreceptor type 22 (PTPN22-R620W) and receptors for the Fc fragment of IgG (FcgRIIa-H131R, FcgRIIIa-F158V FcgRIIIb-NA1/NA2), and rheumatoid arthritis (RA), 133 Tunisian patients with RA and 100 controls were genotyped. We found strong evidence of an association of PTPN22 620W allele and RA. However, analysis does not detect an association between auto-antibodies seropositivity, presence of nodules or erosions and this allele. No significant skewing of any of the three FcgR polymorphisms was seen in this RA group. Nevertheless, we identified FcgRIIIa-V/V158 as the most important FcgR genotype for severe disease subset with joint erosions and observed that patients with FcgRIIIb-NA2/NA2 genotype had an earlier incidence of clinical symptoms. In conclusion, we have confirmed that PTPN22 620W allele is associated with Tunisian RA but does not constitute a factor influencing clinical manifestations. Conversely, this study supports that the FcgRIIa/IIIa and IIIb polymorphisms could influence the course and the severity of this disease. A large number of samples are required to provide independent confirmation of these findings.","PeriodicalId":75537,"journal":{"name":"Archives de l'Institut Pasteur de Tunis","volume":"86 1-4","pages":"51-62"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29186163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence of autoantibodies in a Tunisian cohort of hepatitis C virus infected dialysis patients. 突尼斯丙型肝炎病毒感染透析患者队列中自身抗体的流行

Archives de l'Institut Pasteur de Tunis Pub Date : 2009-01-01

I Sfar, A Elbeldi, S Aouini, A Chiha, H Aouadi, S Ayed-Jendoubi, E Abderrahim, T Ben Abdallah, A Kheder, K Ayed, Y Gorgi

{"title":"Prevalence of autoantibodies in a Tunisian cohort of hepatitis C virus infected dialysis patients.","authors":"I Sfar, A Elbeldi, S Aouini, A Chiha, H Aouadi, S Ayed-Jendoubi, E Abderrahim, T Ben Abdallah, A Kheder, K Ayed, Y Gorgi","doi":"","DOIUrl":"","url":null,"abstract":"The hepatitis C virus (HCV) is the principal agent of viral chronic hepatitis. Cirrhosis and hepatocellular carcinoma are the major complications of this chronic infection. In haemodialysis, HCV infection remains a very frequent problem. Several autoimmune phenomena have been described during this infection. Two hundred haemodialysis patients, all of them anti-HCV (+), were included in this study to evaluate the frequency of Anti-Nuclear Autoantibodies (ANA), anti-cardiolipine antibodies (ACL), anti-smooth muscle antibodies (ASMA), anti-mitochondria antibodies (AMA), anti-thyroperoxydase antibodies (ATPO) and Rheumatoid Factor (RF) comparing them to healthy controls. Sixty eight serums (34%) patients were positive to at least one of the auto-antibodies tested. The difference between patients and controls was statistically significant. These markers were dominated by RF of the IgM isotype and ACL of the IgG isotype. Nevertheless, the positivity of ANA, ASMA, AMA and ATPO was not statistically different comparing to the controls. In addition, an association between the presence of the auto-antibodies and the viral replication was found suggesting that HCV is responsible for inducing these autoimmune phenomena.","PeriodicalId":75537,"journal":{"name":"Archives de l'Institut Pasteur de Tunis","volume":"86 1-4","pages":"63-8"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29186586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[HLA-G: an immunoregulatory non classical class I HLA molecule]. [HLA- g:一种免疫调节非经典I类HLA分子]。

Archives de l'Institut Pasteur de Tunis Pub Date : 2009-01-01

H Ksouri, R Bardi, F Mellouli, M Bejaoui

引用次数: 0

Comparison of hepatitis A seroprevalence in blood donors in South Tunisia between 2000 and 2007. 2000年至2007年南突尼斯献血者甲型肝炎血清阳性率比较

Archives de l'Institut Pasteur de Tunis Pub Date : 2009-01-01

N Louati, L Feki, H Rekik, H Feki, M Chaabouni, A Hammami, J Gargouri, H Karray-Hakim

引用次数: 0

[Mitochondrial DNA: properties and applications]. [线粒体DNA:性质和应用]。

Archives de l'Institut Pasteur de Tunis Pub Date : 2009-01-01

R Kefi-Ben Atig, S Hsouna, E Beraud-Colomb, S Abdelhak

引用次数: 0

[Apoptosis induced by bleomycin: influence of cellular models]. 博来霉素诱导细胞凋亡:对细胞模型的影响。

Archives de l'Institut Pasteur de Tunis Pub Date : 2009-01-01

S Brahim-Loghmari, A Kenani

引用次数: 0

[Chemical synthesis of lactococcin B and functional evaluation of the N-terminal domain using a truncated synthetic analogue]. [乳球菌蛋白B的化学合成和利用截断的合成类似物对n端结构域的功能评价]。

Archives de l'Institut Pasteur de Tunis Pub Date : 2008-01-01

S Lasta, Z Fajloun, P Mansuelle, J M Sabatier, A Boudabous, F Sampieri

引用次数: 0

[A variant of DDRT-PCR using anchored mini-exon primers for identification of differentially expressed sequences in Leishmania infantum]. [一种DDRT-PCR的变体，使用锚定的迷你外显子引物来鉴定婴儿利什曼原虫的差异表达序列]。

Archives de l'Institut Pasteur de Tunis Pub Date : 2008-01-01

L Turki-Mannoubi, H Kbaier-Hachemi, M Barhoumi, A S Chakroun, I Guizani

{"title":"[A variant of DDRT-PCR using anchored mini-exon primers for identification of differentially expressed sequences in Leishmania infantum].","authors":"L Turki-Mannoubi, H Kbaier-Hachemi, M Barhoumi, A S Chakroun, I Guizani","doi":"","DOIUrl":"","url":null,"abstract":"Leishmania infantum (L.i) is responsible for visceral (VL) or cutaneous (CL) leishmaniasis. Previous studies done in Honduras by differential display reverse transcriptase-polymerase chain reaction (DDRT-PCR) failed to demonstrate differences in expression profiles among L. infantum VL and CL parasites. For purpose of comparing expression among L. infantum isolates in Tunisia, a variant of this technique adapted from a commercial kit was developed involving pairs of random and anchored mini-exon primers for isolation and identification of differentially displayed cDNAs. To assess the efficiency of this variant, 34 pairs were applied to 2 consecutive dilutions of cDNAs from promastigotes at end of in vitro exponential growth of 2 visceral (LV50) and cutaneous (DREP14) isolates from Tunisia, thus increasing chance for observing differences among the cDNAs. Profiles were compared and analyzed as regards number and phenotype of bands displayed in 4 types of highly similar amplification profiles among the 2 cDNAs; 26 primer pair combinations generated in total 6.8% differentially displayed bands that had variable intensities or were present/absent, in comparable proportions in the 2 isolates. Analysis further demonstrated differences in amplification efficiency of some primers, emphasizing on qualitative and quantitative impact of relative proximity of the priming sites. Nine present/absent bands were cloned, sequenced and analyzed in silico. Mismatches at priming sites seem to underlie amplification of such bands. Only five products could be referred to annotated gene. Among the genes identified, we list histone H4, largely known to be differentially expressed among L.i stages, and \"NTF2-like\" for which overexpression in one cDNA was here confirmed. To conclude, the variant developed could be used further in Leishmania expression analysis with appropriate cautions about false positives.","PeriodicalId":75537,"journal":{"name":"Archives de l'Institut Pasteur de Tunis","volume":"85 1-4","pages":"29-44"},"PeriodicalIF":0.0,"publicationDate":"2008-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28271151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0