Archives de l'Institut Pasteur de Tunis最新文献_第4页

High rates of macrolide resistance among clinical isolates of Streptococcus agalactiae in Tunisia. 突尼斯无乳链球菌临床分离株大环内酯类药物耐药率高。

Archives de l'Institut Pasteur de Tunis Pub Date : 2010-01-01

M Rachdi, I Boutiba-ben Boubaker, M Hraoui, S Ben Redjeb

{"title":"High rates of macrolide resistance among clinical isolates of Streptococcus agalactiae in Tunisia.","authors":"M Rachdi, I Boutiba-ben Boubaker, M Hraoui, S Ben Redjeb","doi":"","DOIUrl":"","url":null,"abstract":"One hundred sixty non duplicate erythromycin resistant Streptococcus agalactiae isolates were collected in Tunisia from January 2005 to December 2007 They were investigated to determine their resistance level to different macrolides and the mechanisms involved. Most erythromycin resistant S. agalactiae isolates were isolated from urinary specimens (38.75%, 62/160). The constitutive MLSB phenotype (cMLS) showed in 84.3% (135/160) with high MICs of macrolides and lincosamides (MIC90>256 microg/mL) and 8.2% (13/160) inducible MLSB phenotype (iMLS) with high MICs of macrolides (MIC90>256 microg/mL) and moderately increased MICs of lincosamides (MIC90=8 microg/mL). The M phenotype showed in 7.5% (12/160) with moderately increased MICs of macrolides (MIC90=32 microg/mL) and low MICs of lincosamides (MIC90=0.75 microg/mL). All strains were susceptible to quinupristun-dalfopristin association and linezolid (MIC90: 05 and 0.38 microg/mL respectively). Strains with MLSB phenotype harboured erm(B) gene with 825% (n=132), erm(TR) gene with 8.12% (n=13) and erm(B) plus mef (A) with 1.88% (n=3). All strains categorized as M phenotype carried the mef(A) gene (75%, n=12). cMLSB phenotype conferring cross resistance to macrolides, lincosamides and streptogramins B with high level of resistance was the most prevalent.","PeriodicalId":75537,"journal":{"name":"Archives de l'Institut Pasteur de Tunis","volume":"87 1-2","pages":"35-42"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40097421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of specific amino acid sequence (QRRAA) of HLA-DRB1*0405 with rheumatoid arthritis in a Tunisian population. HLA-DRB1*0405特异性氨基酸序列(QRRAA)与突尼斯人群类风湿关节炎的关联

Archives de l'Institut Pasteur de Tunis Pub Date : 2010-01-01

T Dhaouadi, I Sfar, L Abdelmoula, R Bardi, S Jendoubi-Ayed, M Makhlouf, H Aouadi, T Ben Abdallah, R Zouari, K Ayed, Y Lakhoua-Gorgi

{"title":"Association of specific amino acid sequence (QRRAA) of HLA-DRB1*0405 with rheumatoid arthritis in a Tunisian population.","authors":"T Dhaouadi, I Sfar, L Abdelmoula, R Bardi, S Jendoubi-Ayed, M Makhlouf, H Aouadi, T Ben Abdallah, R Zouari, K Ayed, Y Lakhoua-Gorgi","doi":"","DOIUrl":"","url":null,"abstract":"This study aimed to investigate HLA-DRB1 alleles in rheumatoid arthritis (RA) patients from Tunisia and to examine the effect of these alleles on disease severity. HLA-DRBI alleles and sub-typing of DRBI*04 and *01 were determined in 90 patients and 100 healthy controls, by PCR-SSP. HLA-DRB1*04 was significantly higher in patients (51.1%) than in controls (27%) [OR=2.83, p=0.00066]. DRBJ*0405 was found to be the unique DR4 allele associated with RA (28.88% vs 6%) [OR=6.36, p=0.000059]. A significant decrease in the frequency of HLA-DRB1*0701 was observed in RA patients (16.66%) compared to controls (36%) [p=0.0026]. However, the frequency of patients carrying the shared epitope (SE) QRRAA, was slightly increased compared with controls (37.8% vs 23%) [OR=2.03, p=0.039]. We found that the presence of rheumatoid factor, HLA-DR4 and HLA-DRBI*0405 were not significantly associated with bone erosions or the presence of extra-joint involvement. In our population, the SE (QRRAA) expressed in DRBI*04 alleles is related to the susceptibility to RA but it is not involved in RA severity in Tunisia, while DRBI*0701 might protect against this disease.","PeriodicalId":75537,"journal":{"name":"Archives de l'Institut Pasteur de Tunis","volume":"87 1-2","pages":"53-9"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40097422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Abstracts from the Franco-Tunisian Parasitology Meeting, 11-12 November 2010, Tunis]. [法国-突尼斯寄生虫学会议摘要，2010年11月11-12日，突尼斯]。

Archives de l'Institut Pasteur de Tunis Pub Date : 2010-01-01

引用次数: 0

[Immune recovery after allogeneic stem cell transplantation: study of 19 patients]. 同种异体干细胞移植后免疫恢复:19例患者的研究。

Archives de l'Institut Pasteur de Tunis Pub Date : 2010-01-01

E Mellouli, M Ben Khaled, Z Regaya, N Dhouib, M Ouederni, R Kouki, F Jenhani, M Bejaoui

{"title":"[Immune recovery after allogeneic stem cell transplantation: study of 19 patients].","authors":"E Mellouli, M Ben Khaled, Z Regaya, N Dhouib, M Ouederni, R Kouki, F Jenhani, M Bejaoui","doi":"","DOIUrl":"","url":null,"abstract":"The aim of this study was to access average delays for novogeneration of myeloid and lymphoid cells after allogeneic bone marrow transplantation (BMT) outcome and factors affecting this organization. A prospective analysis over 2 years (01/01/07 to 31/12/08) enrolling 19 children treated with allogeneic intrafamilial bone marrow transplantation. Indications for bone marrow transplantation were: aplastic anemia (3 cases), bemoglobinopathies (9 cases), myelodysplastic syndrome (1 case) and primary immunodeficiency (6 cases). Different conditioning regiments were used according to the indication. The study of immune reconstitution was based on the quantitative determination of immunoglobulin and lymphocyte subpopulation. These tests were routinely requested to 1 month, 2 months, 3 months, 6 months, 9 months and 12 months. The average time of engraftment was 18 days (12-24). A rate of CD4+T lymphocytes>200/mm3 was provided within an average of 2,5 months (1-7). The average time to obtain CD8+T lymphocytes>200/mm3 was 2 months (1-5). The humoral immune reconstitution was made within an average of 2 months (1-4). A report of CD4+/CD8+T lymphocytes>I was obtained within 10 months and a half (1-24). Univaried analysis showed a correlation between the bone marrow sex matched and the faster reorganization of CD8+T cells (p=0.042). A quantity of CD34+>6 10(6)/kg was significantly associated with the recapture of a formula lymphocyte CD4+/CD8+T>1 (p=0.03) Immune recovery post bone marrow transplantation in children begins with myeloid lineage then lymphoid B then lymphoid T The inversion of the report CD4+/CD8+T lymphocytes, seems to be influenced by the high contain of CD34+cells in the graft as well as the type of conditioning.","PeriodicalId":75537,"journal":{"name":"Archives de l'Institut Pasteur de Tunis","volume":"87 1-2","pages":"61-8"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40103124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Relationship between temperature and mortality in the city of Tunis: 2005-2007]. [突尼斯城市温度与死亡率的关系:2005-2007]。

Archives de l'Institut Pasteur de Tunis Pub Date : 2010-01-01

J Bettaieb, A Toumi, K Leffondre, N Ben Alaya, A Boukthir, S Chlif, S Hajem, A Ben Salah

引用次数: 0

Presence of STRA-STRB linked streptomycin-resistance genes in clinical isolate of Escherichia coil 2418. 大肠杆菌2418临床分离株中存在STRA-STRB连锁链霉素耐药基因。

Archives de l'Institut Pasteur de Tunis Pub Date : 2010-01-01

K Ben-Mahrez, M Sioud

引用次数: 0

[Neonatal screening of G6PD deficiency in Tunisia]. [突尼斯新生儿G6PD缺乏筛查]。

Archives de l'Institut Pasteur de Tunis Pub Date : 2010-01-01

N Guellouz, I Ben Mansour, M Ouederni, S Jabnoun, S Kacem, Ch Mokrani, R Kastally, M K Chahed, N Khrouf

{"title":"[Neonatal screening of G6PD deficiency in Tunisia].","authors":"N Guellouz, I Ben Mansour, M Ouederni, S Jabnoun, S Kacem, Ch Mokrani, R Kastally, M K Chahed, N Khrouf","doi":"","DOIUrl":"","url":null,"abstract":"The Glucose-6-phosphate dehydrogenase (G6PI) deficiency is the most common enzymopathy worldwide. WHO had classified Tunisia among countries that are moderately affected by this affection. However, no mass-screening reflecting the real incidence was realized. The aim of this study is to determine the prevalence of this enzymopathy and its molecular basis in Tunisia. A total of 1102 neonates, born in CMNT center of Maternity and of Neonatology of Tunis during the going periods from April, 2005 till May, 2005 and from June, 2006 till September, 2006, have been enclosed in the study. The samplings included 953peripheral venous blood and 149 blood cordon. Among 1102 samplings, only 976 were of use to the screening. In our mass-screening, we consider all newborns that were born in the CMNT during the period of study and were included in the screening. A dosage of the enzymatic activity was realized using spectrophotometric method. G6PD electrophoresis and molecular study by PCR/RFLP were realized for the overdrawn newborn children. Among 976 screening neonates, 43 individuals (4.4%) were found to be G6PD deficient by quantitative enzyme assay. Newborn affected were distributed in 23 boys and 20 girls (sex ratio of 1.15). The electrophoretic mobility and the molecular biology were realized for the affected newborn. Molecular characterization of 30 G6PD deficient neonates revealed that the G6PD A- was the most common and was detected in 20 of 43 individuals (66.7%), followed by G6PD Mediterranean that was detected in 6 (13.3%). At least, 4 other unknown mutations were not able to be determined by PCR/RFLP (n=4). In conclusion G6PD deficiency is frequent in our country, justifying a systematic neonatal screening, to avoid the arisen of grave consequences of this affection. The African variant is the most frequent in our country followed by the Mediterranean one.","PeriodicalId":75537,"journal":{"name":"Archives de l'Institut Pasteur de Tunis","volume":"87 1-2","pages":"69-76"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40103035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Snake venom metalloproteinases: structure, biosynthesis and function(s)]. [蛇毒金属蛋白酶:结构、生物合成和功能]

Archives de l'Institut Pasteur de Tunis Pub Date : 2010-01-01

I Limam, M El Ayeb, N Marrakchi

引用次数: 0

[Risk of vertical transmission of hepatitis B virus in Tunisia]. [乙型肝炎病毒在突尼斯垂直传播的风险]。

Archives de l'Institut Pasteur de Tunis Pub Date : 2010-01-01

N Hannachi, O Bahri, N Ben Fredj, J Boukadida, H Triki

{"title":"[Risk of vertical transmission of hepatitis B virus in Tunisia].","authors":"N Hannachi, O Bahri, N Ben Fredj, J Boukadida, H Triki","doi":"","DOIUrl":"","url":null,"abstract":"The risk of vertical transmission of hepatitis B virus (HBV) varies with type of viral endemicity, degree of maternal infection and genomic characteristics of the virus. The aim of this study is to estimate this risk in Tunisia using serological and molecular methods to evaluate HBV replication, to determine viral genotypes and to detect presence of occult hepatitis in 2709 pregnant women. Serological markers were detected by ELISA methods, Genotype was determined by PCR-RFLP and occult hepatitis by nested-PCR. Four percent of women were positive for HBsAg; only 3% of them were also positive for HBeAg. Viral replication, over than 10(3) copies/ml, was detected in 61% of positive HBsAg patients. Three viral genotypes were detected: D (95%), B (3%) and A (3%). Occult hepatitis was detected in 4% of sera with \"anti-HBc isolated\" profile. In conclusion, the risk of vertical transmission of HBV exists in Tunisia. It increases by frequency of precore mutants, predominance of the genotype previously associated with high levels of replication and possibility of occult hepatitis B. These results show the importance of screening by serological HBV markers systematically during pregnancy with evaluation of viral replication in order to prevent vertical risk by efficient tools.","PeriodicalId":75537,"journal":{"name":"Archives de l'Institut Pasteur de Tunis","volume":"87 1-2","pages":"17-24"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40103121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Distribution of surgical hydatidosis in Tunisia, results of 2001-2005 study and trends between 1977 and 2005]. [突尼斯手术包虫病分布，2001-2005年研究结果及1977 -2005年趋势]。

Archives de l'Institut Pasteur de Tunis Pub Date : 2010-01-01

M K Chahed, H Bellali, H Touinsi, R Cherif, Z Ben Safta, M Essoussi, T Kilani

{"title":"[Distribution of surgical hydatidosis in Tunisia, results of 2001-2005 study and trends between 1977 and 2005].","authors":"M K Chahed, H Bellali, H Touinsi, R Cherif, Z Ben Safta, M Essoussi, T Kilani","doi":"","DOIUrl":"","url":null,"abstract":"We have performed an exhaustive retrospective study in all surgical wards (54 services in 35 hospitals) which usually carry out surgical treatment of hydatic cysts in the country, covering the period between January 2001 and December 2005, in order to determine the annual surgical incidence of human cystic hydatidosis in Tunisia. A total of 6249 surgical interventions were recorded during the period 2001-2005. The highest proportion was recorded in the hospitals of Tunis District (42.9%). The service of thoracic surgery from Ariana hospital occupies the first rank (95%). The yearly incidence rate varies between 11 and 13.6 per 100,000. Calculated over the 5 years period, the incidence rate is 63.2 per 100,000 inhabitants, which means an average yearly incidence rate of 12.6 per 100,000 [12.28-12.99]. Governorates of the North West and the Western Central regions of the country are the most endemic area with an average annual incidence rates varying between 19.2 and 33.9 per 100,000, which is at least once and half higher than the national level. After 30 years (1977-2005), the average annual incidence rate slightly dropped, from 15 to 12.6 per 100,000, proving that such zoonosis remains a problem of public health in Tunisia. In order, to control in more or less short term this heavy burden disease and public health expenditure, the only efficient way is the prevention of the diseases with a mass treatment campaign of dogs, principal host of the parasite.","PeriodicalId":75537,"journal":{"name":"Archives de l'Institut Pasteur de Tunis","volume":"87 1-2","pages":"43-52"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40103123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0