{"title":"Kinetics of the humoral immune response to blood-induced falciparum malaria in the squirrel monkey Saimiri sciureus.","authors":"J Gysin, T Fandeur, L Pereira da Silva","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Twenty squirrel monkeys were inoculated with the Palo Alto (FUP) strain of Plasmodium falciparum and treated with quinine one or two weeks after inoculation. Antibody production, measured by the IFA technique, closely followed the appearance of parasites in the blood, rose to a maximum in about two to three weeks, stayed at this level for one to two months and then declined progressively. IgM antibodies appeared faster than any other class of antibody and at two weeks of infection represented 50-80% of total malarial antibodies. IgM antibodies also declined more rapidly and reached barely detectable levels within two to three months. IgG antibodies rose slowly to a maximal level which was, however, maintained for a longer period. No significant differences were observed between intact and splenectomized monkeys. Challenge with virulent parasites two months after the initial infection showed that both groups of monkeys, particularly the intact ones, had acquired a strong protective immunity. The secondary IgG response was stronger when the challenge was performed more than two months after the primo-inoculation.</p>","PeriodicalId":75508,"journal":{"name":"Annales d'immunologie","volume":"133D 1","pages":"95-102"},"PeriodicalIF":0.0,"publicationDate":"1982-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17813472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Study of adjuvant and mitogenic activities of bacterial peptidoglycans with different structures.","authors":"M Guenounou, A F Goguel, C Nauciel","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The adjuvant and mitogenic properties of bacterial peptidoglycans (PG) of different structures have been compared. The PG from Bacillus megaterium and Staphylococcus aureus were adjuvant-active in the guinea-pig, and mitogenic for guinea-pig and mouse lymphocytes. On the other hand the PG from Micrococcus lysodeikticus and Corynebacterium poinsettiae exerted neither adjuvant nor mitogenic activities. Mitogenic PG were selective B mitogens. They were mitogenic for splenocytes from Nude mice and inactive towards T-cell-enriched populations. They were mitogenic in both germ-free mice and in the C3H/HeJ strain which is unresponsive to lipopolysaccharide.</p>","PeriodicalId":75508,"journal":{"name":"Annales d'immunologie","volume":"133D 1","pages":"3-13"},"PeriodicalIF":0.0,"publicationDate":"1982-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17870564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P K Das, A A Ibrahim, F Binkhuysen, P Thi Hué, T H Qui, R Rangarajan, E J Ruitenberg
{"title":"The use of subcellular components of BCG for studying host-Mycobacterium interaction in relation to leprosy.","authors":"P K Das, A A Ibrahim, F Binkhuysen, P Thi Hué, T H Qui, R Rangarajan, E J Ruitenberg","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75508,"journal":{"name":"Annales d'immunologie","volume":"133D 1","pages":"41-59"},"PeriodicalIF":0.0,"publicationDate":"1982-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17813466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"In vitro histamine release from human basophils triggered by a purified allergen from Dermatophagoides farinae: bimodal aspect of the dose response curve.","authors":"A Weyer, J P Dandeu, F Marchand, B David","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Histamine release experiments were performed over a 10(6)-fold range of allergen concentrations on basophils of patients sensitive to Dermatophagoides farinae. Two extracts of D. farinae were used, a partially purified one (Df 80d) containing several allergens and a highly purified component (Ag11). This histamine release patterns obtained with the complex allergen mixture Df 80d gave broad histamine release curves presenting an initial increase, followed by a decrease or a plateau, with occasionally a small dip. The Ag11-induced histamine release curves presented two peaks (bimodal pattern), separated by a more or less pronounced inhibition dip. No non-specific histamine releasing activity was observed with the basophils of two controls. The possible theoretical models for the bimodal histamine release patterns are discussed.</p>","PeriodicalId":75508,"journal":{"name":"Annales d'immunologie","volume":"133D 1","pages":"87-94"},"PeriodicalIF":0.0,"publicationDate":"1982-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17251760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G Konstantinov, M Karacholeva, M Eskenazy, R Ivanova, J Vassileva, F Naumova, R Tekelieva, D Strahilov
{"title":"Passive protection against heterologous gram-negative bacteria mediated by antiserum to epimeraseless Rc mutant of Salmonella minnesota.","authors":"G Konstantinov, M Karacholeva, M Eskenazy, R Ivanova, J Vassileva, F Naumova, R Tekelieva, D Strahilov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Antiserum to the epimeraseless Rc mutant of Salmonella minnesota was prepared by immunization of rabbits. The antiserum provided good protection in mice i.p. challenged with 125 and 25 LD50 doses of live heterologous Gram-negative bacteria i. e. S. typhimurium, Escherichia coli and Pseudomonas aeruginosa. The protection against Klebsiella pneumoniae was negligible. Pre-immune rabbit serum produced only marginal protection. The protective effect of S. minnesota Rc antibodies was entirely abolished upon absorption of Rc antiserum with homologous bacteria. Pre-immune and absorbed sera exhibited a similar protective effect at lower LD50 values, namely 5 and 1 LD50.</p>","PeriodicalId":75508,"journal":{"name":"Annales d'immunologie","volume":"133D 1","pages":"71-6"},"PeriodicalIF":0.0,"publicationDate":"1982-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17813470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Relationship between the development of the intestinal IgA immune system and the establishment of microbial flora in the digestive tract of young holoxenic mice].","authors":"M C Moreau, P Raibaud, M C Muller","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The intestinal villi of axenic mice contain ten-fold less IgA plasmocytes than that of conventional ones. The major stimulus for proliferation of plasma cells synthetizing IgA in the gut of axenic mice is the total microbial flora from adult conventional mice. In young mice, the intestinal IgA immune system (intestinal IgA IS) is fully developed at the age of six weeks. The purpose of this work was to determine the role of intestinal flora on the development of the intestinal IgA IS. To that end, the complete digestive microflora from 1-25 day-old mice was transferred into adult axenic mice. The adult recipient mice harboured the same proportion of the same bacteria as the 1-4 day-old donor mice, Lactobacillus excepted. Thus, development of Lactobacillus is inhibited in recipient mice, whereas we did not observe any inhibition in young donor mice. For the 7-25 day-old donor mice, we still observed some resemblance between the flora of the donors and that of the recipients. However, the number of bacteria belonging to the genera Bacteroides and Eubacterium was larger in recipient than in donor mice. The microflora obtained from 1-23 day-old donor mice did not fully stimulate the development of the intestinal IgA IS of the recipient mice. In contrast, the microflora obtained from a 25 day-old mouse induced a full stimulation of the intestinal IgA IS in the recipient mice. Attempt to isolate the bacteria responsible for this complete stimulation were unsuccessful. We only know that these bacteria are present in a large proportion (10 8/g of faeces), that they are heat-sensible (70 degrees C, 10 min) and bacitracin-sensible. These results showed the important role of the sequential implantation of intestinal microflora in the development of intestinal IgA IS.</p>","PeriodicalId":75508,"journal":{"name":"Annales d'immunologie","volume":"133D 1","pages":"29-39"},"PeriodicalIF":0.0,"publicationDate":"1982-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17941972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Secondary immune response to oral and nasal rough mutant strains of Salmonella typhimurium.","authors":"B Ivanoff, C André, R Fontanges, G Jourdan","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The variations in anti-Salmonella typhimurium antibody levels was measured in BALB/c mice which had been orally and nasally vaccinated with S. typhimurium live mutants of different lipopolysaccharide lengths. Together with the demonstration of immunological memory, the intensity of the secondary response was correlated with the length of the polysaccharide chain at intestinal and serum levels. Nasally administered antigen seemed to be at least as immunogenic as when orally administered.</p>","PeriodicalId":75508,"journal":{"name":"Annales d'immunologie","volume":"133D 1","pages":"61-70"},"PeriodicalIF":0.0,"publicationDate":"1982-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17813468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P Marconi, M Pitzurra, A Vecchiarelli, L Pitzurra, F Bistoni
{"title":"Resistance induced by concanavalin A and phytohaemagglutinin P against tetanus toxin in mice.","authors":"P Marconi, M Pitzurra, A Vecchiarelli, L Pitzurra, F Bistoni","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The effect of concanavalin A (ConA), phytohaemagglutinin P (PHA) and Limulus polyphemus haemocyanin (LPH) on the lethal activity of tetanus toxin (TT) is reported. C3H mice treated s.c. with ConA or PHA but not with LPH from 48 h before to 12 h after s.c. TT challenge showed a significant increase in median survival time compared to control mice inoculated with toxin alone. This protective effect was also obtained when PHA or ConA was administered by the i.p. route, TT being injected s.c. In further studies, mice treated with ConA or PHA by different routes (s.c., i.p. or i.v.) were challenged s.c. with graded minimal lethal doses of TT, with or without i.p. administration of horse antitetanus serum (HATS) 24 h after toxin inoculation. The mice treated with ConA or PHA + HATS showed a significantly increased survival rate and a higher percentage of cured mice with respect to control animals treated with lectins alone. In contrast, the mice challenged with TT and treated with HATS alone did not show any increased survival with respect to untreated controls. Sera from ConA- or PHA-treated mice were unable to neutralize the TT. Immune depression in mice by total-body irradiation (400 R) did not abolish the protective activity of the lectins. These results show that in vivo treatment of mice with ConA or PHA but not with LPH can protect against the lethal effects of TT.</p>","PeriodicalId":75508,"journal":{"name":"Annales d'immunologie","volume":"133D 1","pages":"15-27"},"PeriodicalIF":0.0,"publicationDate":"1982-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17813582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A F Pestana De Castro, J A Gomes, M B Serafim, D Queiroz Tavares, M H Lemos Dos Reis
{"title":"Permeability factor-like skin reaction in rabbits induced by endotoxin of Salmonella sp.","authors":"A F Pestana De Castro, J A Gomes, M B Serafim, D Queiroz Tavares, M H Lemos Dos Reis","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>An inflammatory skin reaction similar to the permeability factor (PF) described for the thermolabile (TL) enterotoxin of Escherichia coli was induced in rabbits inoculated intradermally with supernatants from cultures of Salmonella typhimurium and S. enteritidis. This PF-like activity was observed with both crude supernatants as well as those which were submitted to gel filtration through Sephadex G-100. PF-like activity was found only in fraction 1 (F1) of the chromatographed materials. It was resistant to boiling, proteolytic enzymes and wide variations of pH. Serological studies based on agglutination and immunodiffusion tests demonstrated that F1 materials were closely related to the somatic antigen of group B Salmonella. No specific TL activity, as detected by the Y-1 adrenal cell assay and the passive immune haemolysis test, could be demonstrated. Furthermore, F1 materials were not enterotoxigenic as assayed by the rabbit ileal loop assay, and no neutralization of PF-like activity could be obtained in tests carried out using F1 preparations pre-incubated with either anti-F1 or cholera antitoxin. Based upon these findings, it seems reasonable to suppose that most PF reactions, already reported as being caused by a TL-like enterotoxin produced by Salmonella, are probably due to endotoxin. In fact, this possibility was reinforced by the Sanarelli-Shwartzman phenomenon which was produced in rabbits inoculated with F1 materials.</p>","PeriodicalId":75508,"journal":{"name":"Annales d'immunologie","volume":"133D 1","pages":"103-17"},"PeriodicalIF":0.0,"publicationDate":"1982-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17813580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Preparation of monospecific anti-Salmonella LPS antibodies from bacteria immobilized in polyacrylamide gel].","authors":"J Goichot, M Duphot","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The use of an immunoabsorbent obtained by entrapping Salmonella cells into a polyacrylamide gel lattice enabled us to obtain anti-O monospecific immune sera which can be used for a quick serological identification of some species of Salmonella in the course of a diagnosis. In this paper we describe a method for immobilization of S. typhimurium as well as the preparation of monospecific anti-05 antibodies from plurispecific anti-S. haifa rabbit immune sera. This separation of anti-O monospecific antibodies avoids the repeated and often tedious adsorption of anti-Salmonella immune sera by the corresponding whole bacteria. Such Salmonella, entrapped into the polyacrylamide gel lattice, can be used several times without appreciable loss of their affinity properties.</p>","PeriodicalId":75508,"journal":{"name":"Annales d'immunologie","volume":"133C 3","pages":"383-6"},"PeriodicalIF":0.0,"publicationDate":"1982-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17811178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}