Allergy, Asthma & Immunology Research最新文献

{"title":"Real-World Investigation of Eosinophilic-Associated Disease Overlap (REVEAL): Analysis of a US Claims Database.","authors":"Anamaria Brailean,&nbsp;Justin Kwiatek,&nbsp;Danuta Kielar,&nbsp;Rohit Katial,&nbsp;Xia Wang,&nbsp;Xiao Xu,&nbsp;Yong Jin Kim,&nbsp;Michael Stokes,&nbsp;Heide A Stirnadel-Farrant","doi":"10.4168/aair.2023.15.5.580","DOIUrl":"10.4168/aair.2023.15.5.580","url":null,"abstract":"<p><strong>Purpose: </strong>The epidemiology of eosinophil-associated diseases (EADs) is not yet fully understood. While some studies have been conducted on stand-alone eosinophilic diseases, there is scarce evidence on the degree of overlap among rarer conditions.</p><p><strong>Methods: </strong>The retrospective Real-world inVestigation of Eosinophilic-Associated disease overLap (REVEAL) study used data from the Optum^® Clinformatics^® insurance claims database to describe and characterize disease overlap among 11 EADs: allergic bronchopulmonary aspergillosis, atopic dermatitis, chronic rhinosinusitis with nasal polyps, eosinophilic gastritis/gastroenteritis, eosinophilic granulomatosis with polyangiitis, eosinophilic esophagitis, bullous pemphigoid, chronic obstructive pulmonary disorder, chronic spontaneous urticaria, and non-cystic fibrosis bronchiectasis. Patient records with EADs of interest were identified between January 1, 2015, and June 30, 2018.</p><p><strong>Results: </strong>Overall, 1,326,645 patients were included; 74.4% had 1 EAD, 20.5% had ≥ 2 EADs, and 5.1% had ≥ 3 EADs. Higher rates of disease overlap were associated with older age. Higher blood eosinophil counts were also observed in patients with a greater number of overlapping conditions, suggesting a common role for eosinophilic inflammation in the pathogenesis of multiple diseases. Furthermore, greater disease overlap was associated with higher disease severity in most cohorts.</p><p><strong>Conclusions: </strong>Results from this study have implications for quantifying unmet needs and can be used to inform treatment guidelines and raise the awareness of eosinophilic inflammation and EAD overlap among healthcare professionals from a range of disease specialties.</p>","PeriodicalId":7547,"journal":{"name":"Allergy, Asthma & Immunology Research","volume":"15 5","pages":"580-602"},"PeriodicalIF":4.4,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/bf/be/aair-15-580.PMC10570778.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41188258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of TRIM24 in Allergic Rhinitis.","authors":"Seung Koo Yang,&nbsp;Doo Hee Han","doi":"10.4168/aair.2023.15.5.543","DOIUrl":"10.4168/aair.2023.15.5.543","url":null,"abstract":"https://e-aair.org Allergic rhinitis (AR) is a common inflammatory disease characterized by rhinorrhea, nasal obstruction, sneezing, and itchy nose. The inflammation of nasal mucosa is ultimately caused by an exposure to allergens and immunoglobulin (Ig) E-mediated sensitization, in which T helper type 2 (Th2) cells and cytokines—interleukin (IL)-4, IL-5, and IL-13—play an important role.1,2 IL-4 is crucial in activating Janus kinase (JAK), which phosphorylates transcription factor, signal transducer and activator of transcription 6 (STAT6), a key factor for Th2 polarization.3,4 On the other hand, tripartite motif-containing 24 (TRIM24), promotes STAT6 acetylation by catalyzing the ubiquitination of cAMP-responsive elementbinding protein (CREB)-binding protein at Lys 119.5 Previous studies have proposed the role of TRIM24 in other diseases, such as head and neck squamous cell carcinoma,6 prostate cancer,7 and breast cancer.8 However, its role in AR has not been explored.","PeriodicalId":7547,"journal":{"name":"Allergy, Asthma & Immunology Research","volume":"15 5","pages":"543-544"},"PeriodicalIF":4.4,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/bc/cf/aair-15-543.PMC10570779.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41188259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Atopic Dermatitis and the Risk of Myocardial Infarction and All-Cause Mortality: A Nationwide Population-Based Cohort Study.","authors":"Yu Ri Woo,&nbsp;Minah Cho,&nbsp;Kyung Do Han,&nbsp;Sang Hyun Cho,&nbsp;Ji Hyun Lee","doi":"10.4168/aair.2023.15.5.636","DOIUrl":"10.4168/aair.2023.15.5.636","url":null,"abstract":"<p><strong>Purpose: </strong>Atopic dermatitis (AD) is a chronic inflammatory skin disorder associated with various comorbidities. However, inconsistent results on the risk of myocardial infarction (MI) and mortality have been reported in patients with AD. This study was aimed to evaluate the risk of MI and all-cause mortality in patients with AD.</p><p><strong>Methods: </strong>This nationwide population-based retrospective cohort study enrolled 56,205 adults ≥ 20 years of age with AD and 3,825,609 controls without AD from the Korean National Health Service (NHIS) database from 2009 to 2016.</p><p><strong>Results: </strong>The risk of MI (adjusted hazard ratio [aHR], 1.111, 95% confidence interval [CI], 1.050-1.176) was increased in patients with AD. By AD severity, patients with moderate-to-severe AD had a higher risk of MI (aHR, 1.163, 95% CI, 1.080-1.251) than individuals without AD. The risk of all-cause mortality was only increased for patients with moderate-to-severe AD (aHR, 1.096, 95% CI, 1.040-1.155) compared to individuals without AD. In subgroup analysis, an increased risk of MI was observed in female, non-obese, non-smoking, non-diabetic, and non-dyslipidemic patients with moderate-to-severe AD compared to individuals without AD. An increased risk of all-cause mortality was observed in patients with moderate-to-severe AD compared to non-AD controls among individuals ≥60 years of age and non-smokers.</p><p><strong>Conclusions: </strong>The risk of MI and all-cause death was increased in patients with moderate-to-severe AD. Even without well-known risk factors for MI and mortality, patients with AD require the proper management and screening for comorbidities to prevent MI and decrease all-cause mortality.</p>","PeriodicalId":7547,"journal":{"name":"Allergy, Asthma & Immunology Research","volume":"15 5","pages":"636-646"},"PeriodicalIF":4.4,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b1/15/aair-15-636.PMC10570776.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41188241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eosinophilic-Associated Disease Overlap: What Do We Know About It?","authors":"Noeul Kang,&nbsp;Tae-Bum Kim","doi":"10.4168/aair.2023.15.5.539","DOIUrl":"10.4168/aair.2023.15.5.539","url":null,"abstract":"https://e-aair.org Over the past two decades, it was discovered that a range of inflammatory diseases involving several organ systems with elevated eosinophil counts in blood and/or tissue was primarily driven by abnormal regulation of the number and activation state of eosinophils.1-11 Eosinophil-associated diseases (EADs) refer to these heterogeneous conditions in which eosinophils are believed to play critical pathological roles.1,12-14 EADs encompass common respiratory and dermatologic conditions, such as asthma,3-5 chronic rhinosinusitis with nasal polyps,6 and atopic dermatitis,7 less common eosinophilic gastrointestinal diseases,8 and rare conditions including allergic bronchopulmonary aspergillosis (ABPA),9 eosinophilic granulomatosis with polyangiitis (EGPA)1 and hypereosinophilic syndromes (HES).10","PeriodicalId":7547,"journal":{"name":"Allergy, Asthma & Immunology Research","volume":"15 5","pages":"539-542"},"PeriodicalIF":4.4,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b7/60/aair-15-539.PMC10570782.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41188245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association Between Nasal Colonization of <i>Staphylococcus aureus</i> and Eczema of Multiple Body Sites.","authors":"Yang Guo,&nbsp;Xia Dou,&nbsp;Xiao-Fan Chen,&nbsp;Cong Huang,&nbsp;Ying-Jie Zheng,&nbsp;Bo Yu","doi":"10.4168/aair.2023.15.5.659","DOIUrl":"10.4168/aair.2023.15.5.659","url":null,"abstract":"<p><strong>Purpose: </strong><i>Staphylococcus aureus</i> is the critical pathogenic bacterium of eczema. The relationship between nasal colonization by <i>S. aureus</i> and eczema has not been well studied. We aimed to evaluate the associations between nasal colonization by <i>S. aureus</i> and eczema of multiple body sites, including persistent and ever-reported eczema. We further examined the associations between eczema and different subtypes of <i>S. aureus</i>, that is, methicillin-resistant <i>S. aureus</i> (MRSA) and methicillin-sensitive <i>S. aureus</i> (MSSA).</p><p><strong>Methods: </strong>The real-world data from the US National Health and Nutrition Examination Survey were used. The associations were calculated using survey-weighted multinomial logistic regression models and further calculated in subgroups stratified by demographic factors.</p><p><strong>Results: </strong>In total, 2,941 adults were included. The prevalence rate of <i>S. aureus</i> nasal carriage was significantly higher in adults with persistent hand eczema (51.0%) than in those with ever-reported hand eczema (23.3%) and never eczema (26.9%). <i>S. aureus</i> nasal colonization was associated with an approximately two-fold increased risk of persistent hand eczema (odds ratios ranges in different models: 2.86-3.06) without significant heterogeneity in the association by demographic factors. No significant associations between <i>S. aureus</i> nasal colonization and persistent eczema of other body sites or ever-reported eczema of multiple body sites (including hands) were observed. Furthermore, similar significant association between nasal colonization of MSSA and persistent hand eczema was seen; the association was much stronger (odds ratios ranges in different models: 4.64-6.54) for MRSA, although with borderline significant.</p><p><strong>Conclusions: </strong>Nasal colonization of <i>S. aureu</i>s was associated with increased risk of persistent hand eczema. Our findings imply that preventive measures targeting <i>S. aureus</i> for the anterior nares should be considered in preventing and treating eczema.</p>","PeriodicalId":7547,"journal":{"name":"Allergy, Asthma & Immunology Research","volume":"15 5","pages":"659-672"},"PeriodicalIF":4.4,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f7/8e/aair-15-659.PMC10570784.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41188240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacterial Etiology in Subacute Cough.","authors":"Ji-Yong Moon,&nbsp;Hyun Lee,&nbsp;Min-Hye Kim,&nbsp;Woo-Jung Song,&nbsp;Sang Min Lee,&nbsp;Sae-Hoon Kim,&nbsp;Sang-Heon Kim,&nbsp;Byung-Jae Lee,&nbsp;Ho Joo Yoon,&nbsp;Sang Hoon Kim","doi":"10.4168/aair.2023.15.5.673","DOIUrl":"https://doi.org/10.4168/aair.2023.15.5.673","url":null,"abstract":"<p><p>Although postinfectious etiology is the most common cause of subacute cough, there are insufficient data on the epidemiology of associated bacterial infections. We aimed to identify the etiology of bacterial detection in subjects with subacute cough. A multicenter prospective observational study of 142 patients with postinfectious subacute cough was performed between August 2016 and December 2017 in Korea. We obtained 2 nasal swabs from each patient and used a multiplex bacterial polymerase chain reaction (PCR) kit that simultaneously detects <i>Bordetella pertussis</i>, <i>Chlamydophila pneumoniae</i>, <i>Haemophilus influenzae</i>, <i>Legionella pneumophilia</i>, <i>Mycoplasma pneumoniae</i>, and <i>Streptococcus pneumoniae</i>. About 29% (n = 41) of patients with subacute cough were positive for bacterial PCR in nasal swabs. The most common bacteria detected by bacterial PCR was <i>H. influenzae</i> (n = 19, 13.4%), followed by <i>S. pneumoniae</i> (n = 18, 12.7%), <i>B. pertussis</i> (n = 7, 4.9%), <i>M. pneumoniae</i> (n = 3, 2.1%), <i>L. pneumophilia</i> (n = 2, 1.4%), and <i>C. pneumoniae</i> (n = 1, 0.7%). Nine patients had dual positivity for the PCR. In conclusion, bacterial PCR was positive in the nasal swabs of about 29% of subjects with subacute cough, including 5% of positive PCR results for <i>B. pertussis.</i></p>","PeriodicalId":7547,"journal":{"name":"Allergy, Asthma & Immunology Research","volume":"15 5","pages":"673-681"},"PeriodicalIF":4.4,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/02/6c/aair-15-673.PMC10570775.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41188242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunonutrition: Diet Diversity, Gut Microbiome and Prevention of Allergic Diseases.","authors":"Carina Venter","doi":"10.4168/aair.2023.15.5.545","DOIUrl":"10.4168/aair.2023.15.5.545","url":null,"abstract":"<p><p>Allergic diseases are increasing both in morbidity and mortality. Genetic, environmental, and dietary factors may all be involved in this increase. Nutrition during pregnancy, breastfeeding, and early life may play a particularly important role in preventing allergic diseases. Based on current systematic reviews, the intake of specific nutrients has failed to prevent allergic disease. Prevention strategies have shifted their focus to the overall diet which can be described using diet diversity. Infant and maternal diet diversity in pregnancy has been associated with reduced allergy outcomes in childhood. Overall, diet also seems to have a marked effect on the microbiome compared to single foods. Factors that may negate the allergy-preventative effect of overall diet diversity include the addition of emulsifiers, advanced glycation end-product content, and overuse of commercial baby foods. There is a need to perform randomized controlled trials using overall dietary intake to support international food allergy guidelines. These studies should ideally be conducted by multi-professional teams.</p>","PeriodicalId":7547,"journal":{"name":"Allergy, Asthma & Immunology Research","volume":"15 5","pages":"545-561"},"PeriodicalIF":4.4,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c5/83/aair-15-545.PMC10570780.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41188247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TRIM24-Mediated Acetylation of STAT6 Suppresses Th2-Induced Allergic Rhinitis.","authors":"Liyan Yue,&nbsp;Qiaojing Jia,&nbsp;Jinhui Dong,&nbsp;Jianxing Wang,&nbsp;Xiumin Ren,&nbsp;Ou Xu","doi":"10.4168/aair.2023.15.5.603","DOIUrl":"10.4168/aair.2023.15.5.603","url":null,"abstract":"<p><strong>Purpose: </strong>Allergic rhinitis (AR) is a T helper type 2 (Th2)-mediated inflammatory disease. The E3 ligase tripartite motif-containing 24 (TRIM24) regulates the recruitment of acetyltransferase CREB-binding protein (CBP) to signal transducer and activator of transcription 6 (STAT6). CBP mediates the acetylation of STAT6 and decreases its activity. To date, the precise role of TRIM24 in AR has not been fully interpreted. Herein, our study aimed to explore the functions of TRIM24 in AR.</p><p><strong>Methods: </strong>The expression of TRIM24 in peripheral blood mononuclear cells (PBMCs) and CD4⁺ T cells from patients with AR was measured. TRIM24-conditional knockout mice with TRIM24 deficiency in CD4⁺ T cells were generated. Wide-type (WT) AR mice and TRIM24-conditional knockout AR mice were established. Then, AR symptoms and interleukin (IL)-4 levels were compared. Further, the proliferation, activation and polarization of CD4⁺ T cells from WT mice and TRIM24 knockout mice after stimulation were determined. The effects of TRIM24 deficiency on STAT6 activities were also evaluated.</p><p><strong>Results: </strong>Downregulated TRIM24 expression was detected in PBMCs and CD4⁺ T cells from patients with AR. TRIM24 conditional knockout mice had more sever AR symptoms with elevated IL-4 production. TRIM24-knockout CD4⁺ T cells had similar proliferation and activation when compared to WT CD4⁺ T cells, while they had enhanced Th2 polarization. TRIM24-knockout CD4⁺ T cells had decreased acetylation of STAT6 and enhanced STAT6 activities after IL-4 stimulation. The regulation of STAT6 activities by TRIM24 depended on TRIM24 N terminal RIGN domain and Lys383 acetylation site of STAT6.</p><p><strong>Conclusions: </strong>TRIM24 suppresses Th2-mediated AR by regulating the acetylation of STAT6.</p>","PeriodicalId":7547,"journal":{"name":"Allergy, Asthma & Immunology Research","volume":"15 5","pages":"603-613"},"PeriodicalIF":4.4,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/2b/b7/aair-15-603.PMC10570786.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41188260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Causal Link between Human Blood Metabolites and Asthma: An Investigation Using Mendelian Randomization","authors":"Yong-Qing Zhu, Xiao-Yan Meng, Jing-Hua Yang","doi":"10.29328/journal.aaai.1001032","DOIUrl":"https://doi.org/10.29328/journal.aaai.1001032","url":null,"abstract":"Background: Asthma, a chronic inflammatory respiratory ailment, is characterized by variable airflow obstruction and heightened bronchial reactivity. Despite therapeutic advancements, a comprehensive comprehension of its underlying metabolic mechanisms remains elusive. Metabolomics has emerged as a powerful approach to investigating the complex connections between serum metabolites and disease pathogenesis. However, exploring the causal relationship between serum metabolites and asthma susceptibility demands meticulous examination to unveil potential therapeutic targets. Methods: Mendelian randomization (MR) approach was explored to investigate the potential causal associations between serum metabolites and asthma risk. The main analysis employed the inverse variance weighted method, supported by supplementary approaches such as MR-Egger, weighted median, weighted mode, and sample mode. To enhance the strength and credibility of our results, we conducted sensitivity analyses encompassing heterogeneity testing, assessment of horizontal pleiotropy, and leave-one-out analysis. Additionally, pathway enrichment analysis was performed to further elucidate the results. Results: We identified 18 known and 12 unknown metabolites with potential associations with asthma risk. Among known metabolites, seven exhibited protective effects (e.g., 4-acetamidobutanoate, allantoin, kynurenine, oxidized bilirubin*), while eleven were considered risk factors (e.g., ornithine, N-acetylornithine, alanine). Through the integration of four additional MR models and sensitivity analyses, we revealed a connection between 4-acetamidobutanoate and approximately 6% lower asthma risk (OR = 0.94, 95% CI: 0.90–0.98). Conclusions: Our MR analysis uncovered protective and risk-associated metabolites, alongside 12 unknown metabolites linked to asthma. Notably, 4-acetamidobutanoate demonstrated a nominal 6% reduction in asthma risk, highlighting its potential significance.","PeriodicalId":7547,"journal":{"name":"Allergy, Asthma & Immunology Research","volume":"110 1","pages":""},"PeriodicalIF":4.4,"publicationDate":"2023-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81578558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predictive Significance of Claudin-3 for Epithelial Barrier Dysfunction in Chronic Rhinosinusitis With Nasal Polyps.","authors":"Zhi-Qun Huang,&nbsp;Jing Ye,&nbsp;Jing Liu,&nbsp;Li-Ying Sun,&nbsp;Hsiao Hui Ong,&nbsp;Yong-Hao Wei,&nbsp;Shu-Cai Fu,&nbsp;Xiao-Xun Hu,&nbsp;Yu Xu,&nbsp;De-Yun Wang","doi":"10.4168/aair.2023.15.4.512","DOIUrl":"https://doi.org/10.4168/aair.2023.15.4.512","url":null,"abstract":"<p><strong>Purpose: </strong>The abnormal expression of tight junction (TJ) plays a vital role in the pathogenesis of chronic rhinosinusitis with nasal polyps (CRSwNP). However, there is no appropriate tool to distinguish and diagnose epithelial barrier defects in clinical practice. This study aimed to evaluate the predictive value of claudin-3 for epithelial barrier dysfunction in CRSwNP.</p><p><strong>Methods: </strong>In this study, TJ protein levels were evaluated by real-time quantitative polymerase chain reaction, immunofluorescent, and immunohistochemistry staining in control subjects and CRSwNP patients. The receiver operating characteristic (ROC) curve was created to assess the predictive value of TJ breakdown in clinical outcomes. <i>In vitro,</i> human nasal epithelial cells were cultured at the air-liquid interface to analyze the transepithelial electrical resistance (TER) level.</p><p><strong>Results: </strong>The expression levels of occludin, tricellulin, claudin-3, and claudin-10 were decreased (all <i>P</i> < 0.05), and those of claudin-1 was increased (<i>P</i> < 0.05) in CRSwNP patients as compared to healthy subjects. Additionally, claudin-3 and occludin levels were negatively correlated with the computed tomography score in CRSwNP (all <i>P</i> < 0.05), and the ROC curve indicated that the claudin-3 level had the most predictive accuracy in evaluating epithelial barrier disruption (area under the curve = 0.791, <i>P</i> < 0.001). Finally, the time-series analysis showed the highest correlation coefficient between TER and claudin-3 (cross-correlation function = 0.75).</p><p><strong>Conclusion: </strong>In this study, we suggest that claudin-3 could be a valuable biomarker for predicting nasal epithelial barrier defects and disease severity in CRSwNP.</p>","PeriodicalId":7547,"journal":{"name":"Allergy, Asthma & Immunology Research","volume":"15 4","pages":"512-525"},"PeriodicalIF":4.4,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/da/70/aair-15-512.PMC10359644.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9852534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}