Y Shiotsuka, S Hayashi, M Hayashi, T Sahashi, K Kobayashi, M Sase, S Takada, T Katsunuma
{"title":"[A study on the mechanism of production and secretion of HPL (author's transl)].","authors":"Y Shiotsuka, S Hayashi, M Hayashi, T Sahashi, K Kobayashi, M Sase, S Takada, T Katsunuma","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Our preliminary data suggest that HPL was present in the largest quantity in chorionic villi of 16-18 weeks of pregnancy but the concentration of HPL in mother blood was maximum at 30-40 weeks of pregnancy. To clarify the discrepancy of term of those maximum values, the following experiments were done. The immuno-precipitate caused by the reaction with anti-HPL serum in the crude extract from chorionic villi incubated with MEM medium containing [3H]-leucine was subjected to SDS gel electrophoresis. Three radioactive bands were observed. Their molecular weights were 22000, 29000 and 48000, native HPL is 22000 molecular weights and other heavier molecular weights protein are surmised pre- or pro-HPL. Native HPL was few in 30 min. incubated cases but increased in over night incubated cases. Other heavier molecular weights protein changed into native HPL with the lapse of time. The concentration of HPL in mother blood is surmised to reflect the production of two pro-HPL is syncytium cells, change into free HPL and furthermore their secretion.</p>","PeriodicalId":75398,"journal":{"name":"Acta obstetrica et gynaecologica Japonica","volume":"33 6","pages":"819-24"},"PeriodicalIF":0.0,"publicationDate":"1981-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18259692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M Higuchi, H Hirano, H Takahashi, O Mochizuki, M Maki, M Hasegawa
{"title":"[Human fetal breathing movements: basic analysis and clinical relationship to fetal well-being (author's transl)].","authors":"M Higuchi, H Hirano, H Takahashi, O Mochizuki, M Maki, M Hasegawa","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>By means of real time B-scan, human fetal breathing movements (FBM) was basically and clinically investigated on 244 observations in 215 pregnancies. The results obtained were as follows. (1) FBM was frequently observed during active sleep in which FHR variability and fetal movement increased. (2) The incidence of FBM increased rapidly from 12.5% at 23-27 weeks to 77-100% at more than 35 weeks gestation, but reduced to 44% at delivery. (3) FBM became more regular and deeper with advancing weeks of pregnancy, especially after 36 weeks of gestation. The frequency of FBM in normal pregnancy was slightly high at 28-29 weeks, but almost the same rates at 30-41 weeks. (4) Maternal mean blood glucose level of 6 cases (72.3 +/- 5.8 mg/dl) in which FBM absent in repeated observations was significantly lower than those of 10 cases (94.7 +/- 14 mg/dl) in which the percentage time spent breathing were 50% or more. (5) Clinically, a relationship between the presence or absence of FBM and Apgar score was not recognized. However, when the percentage time spent breathing was less than 30% or FBM was continuous and considerably deep, the incidence of neonatal asphyxia or FHR abnormality during labor was significantly increased.</p>","PeriodicalId":75398,"journal":{"name":"Acta obstetrica et gynaecologica Japonica","volume":"33 5","pages":"597-606"},"PeriodicalIF":0.0,"publicationDate":"1981-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18248888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N Umesaki, J Nakade, H Nakamori, H Sako, T Lee, T Sugawa
{"title":"[Study of monocyte function in patients with cervical cancer by using T-lymphocyte monocyte interaction in response to phytohemagglutinin (author's transl)].","authors":"N Umesaki, J Nakade, H Nakamori, H Sako, T Lee, T Sugawa","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Monocytes have been thought to play an important role in the immune surveillance system of cancer patients since monocytes were shown to participate as accessory cells in the induction of T-cell proliferation to PHA by Potter et al. in 1977. We had already reported the depression of monocyte function with the progress of cancer after PHA response of mononuclear cells obtained by centrifugation of Ficoll-Hypaque and lymphocytes obtained by depletion of monocytes ingesting Silica from mononuclear cells was compared in cancer patients. In that study, however, monocyte function was considered not to be indicated truly because responding cells were different at each examination. Therefore, in this study responding cells were graded as T-cells obtained from healthy volunteers and monocytes were added to the T-cells. Monocyte function in this study was as follows: PHA response to T-cells and monocytes over PHA response to T-cells. In this study, monocytes were allogenic to T-cells. At first, the influence of monocytes to allogenic T-cells was studied but it was found to be negligible. The ratio of monocytes and T-cells was 1:1. The concentration of PHA was 20 micrograms/ml. Finally, the monocyte function of patients with cervical cancer was studied. It was increased at the stage of CIS and then gradually decreased. At the advanced stage, it was significantly decreased to comparison with that of healthy volunteers.</p>","PeriodicalId":75398,"journal":{"name":"Acta obstetrica et gynaecologica Japonica","volume":"33 5","pages":"664-70"},"PeriodicalIF":0.0,"publicationDate":"1981-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18021037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A clinicopathological study on glassy cell carcinoma of the cervix.","authors":"Y Tsukahara, Y Sakai, J Ishii, S Iwai, T Fukuta","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>From the present investigation, it was verified that glassy cell carcinoma is a clinicopathologically independent tumor. As a result, the following conclusions are drawn: 1. The frequency of glassy cell carcinoma is 1.3% of all cervical carcinomas. 2. The prognosis of this type of tumor is poor. Death occurred in 13 of 14 cases studied. The survival period was an average of 16.1 months. 3. The following factors contribute to poor prognosis of this type of tumor: rapid growth, readiness for distant metastasis, and resistance to irradiation. 4. Histologically, undifferentiated tumor cells with distinct nucleoli, including many mitotic figures, form a mantle-like layer around small blood vessels. Cells characterized by the presence of cytoplasm with a ground-glass appearance are found outside this layer. 5. The authors have proposed a form of carcinoma possessing the characteristics of both squamous cell carcinoma and adenocarcinoma, and called it an intermediate group. Glassy cell carcinoma seems to be classified into a subtype of this group. Some other subtypes closely related to this carcinoma were noticed. 6. Tumors to be distinguished from glassy cell carcinoma, large cell, non-keratinizing squamous cell carcinoma and lobular type carcinoma were pointed out in this report.</p>","PeriodicalId":75398,"journal":{"name":"Acta obstetrica et gynaecologica Japonica","volume":"33 5","pages":"699-704"},"PeriodicalIF":0.0,"publicationDate":"1981-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18247751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A sex difference of the concentrations of gonadotropins, its subunits and sex steroids in cord veins.","authors":"U Miyamoto","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In order to elucidate the sex difference of cord blood hormones, concentration of hCG, hCG-alpha, hCG-beta, hLH, hFSH, estradiol (E2), progesterone (P) and testosterone (T) in cord veins were assayed by radioimmunoassays. Mean concentrations of cord vein hCG, hCG-alpha and hLH were significantly higher in female than in male, but those of hFSH and T were significantly higher in male than in female. Male and female cord vein levels of hCG-beta, E2 and P were not significantly different and hCG-beta and hFSh levels in cord veins at term are low in both sexes. These lower concentrations of hLH in male may be a result of the feedback inhibitory action by the higher concentrations of T in male at term. These data suggest a sex difference in the synthesis and secretion of gonadotropins by the pituitary gland in the human foetus at term.</p>","PeriodicalId":75398,"journal":{"name":"Acta obstetrica et gynaecologica Japonica","volume":"33 5","pages":"711-3"},"PeriodicalIF":0.0,"publicationDate":"1981-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17838036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Studies on the mechanism and the significance of prostaglandin biosynthesis by the ovary--conversion of 14C-arachidonic acid to prostaglandins (author's transl)].","authors":"N Mitsuhashi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Biosynthesis of prostaglandins (PGs) from 14C-arachidonic acid was studied using homogenates of the ovaries from rats, gilts and human. Among the radioactive metabolites extracted, PGE2 and 6 keto PGF1 alpha were purified and identified by silicic acid column-, thin layers-, reversed phase partition chromatographies, and radiogaschromatography. Treatment with PMS-hCG activated metabolism of arachidonic acid into radioactive metabolites including PGE2 and 6 keto PGF1 alpha to a large extent. Therefore it is concluded that LH or (hCG) activate the PG synthetase system through the induction of synthetase itself and/or a stimulatory factor.</p>","PeriodicalId":75398,"journal":{"name":"Acta obstetrica et gynaecologica Japonica","volume":"33 5","pages":"591-6"},"PeriodicalIF":0.0,"publicationDate":"1981-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18249054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Committee report on nutrition and metabolism in gyneco-obstetrics (Survey on pregnancy in diabetes mellitus in Japan)].","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75398,"journal":{"name":"Acta obstetrica et gynaecologica Japonica","volume":"33 5","pages":"731-40"},"PeriodicalIF":0.0,"publicationDate":"1981-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18247754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Committee report on neonatal care registration (neonatal mortality registration in 1978)].","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75398,"journal":{"name":"Acta obstetrica et gynaecologica Japonica","volume":"33 5","pages":"715-29"},"PeriodicalIF":0.0,"publicationDate":"1981-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18247753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Urinary total estrogens in normal menstrual cycle and early pregnancy (author's transl)].","authors":"T Yamane, M Tatsumura, S Uzu, H Kawamoto","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A ratio of urinary total estrogens to creatinine (E/C, micrograms/g) was measured in every morning through the cycle in normal menstruating cycling women and during her early pregnancy. The level of follicular phase was 17.7 +/- 5.4 micrograms/g, ovulatory phase 78.8 +/- 8.8 micrograms/g and luteal phase 26.7 +/- 11.2 micrograms/g. In the spontaneous pregnancies E/C levels elevated sharply after 15 weeks of gestation. In the pregnancy after induced ovulation with clomifene citrate and conjugated estrogens, E/C levels were decreased from 7 days prior to the initiation of genital bleeding of spontaneous abortion. The need for multiple determinations over considerable periods of time is solved by our methods of easily repeated sequential measurements of urinary total estrogens estimated by E/C ratio.</p>","PeriodicalId":75398,"journal":{"name":"Acta obstetrica et gynaecologica Japonica","volume":"33 5","pages":"619-24"},"PeriodicalIF":0.0,"publicationDate":"1981-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18248890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Studies on the placental damage and IUGR caused by intravaginally inoculated herpes simplex virus type-2 (HSV-2) during pregnancy (author's transl)].","authors":"Y Ando","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>It has been recognized that intrauterine growth retardation (IUGR) could be caused by viral infection during pregnancy. We have experimentally attempted the intravaginal inoculation of HSV-2 (UW-268) 1.2-2.0 X 10(6) plaque forming unit in the pregnant rat. HSV-2 inoculated at 8th and 11th day of gestation, the virus invaded in the basal layer of placenta after 15th day of gestation, but no evidence of intrauterine infection on fetus was identified. The invading route of the virus was investigated by the use of autoradiography. The effect of the virus for fetal growth retardation and placental damage was studied by the use of 14C-leucine, 14C-alanine and 3H-glucose. The virus ascended in the myometrium and endometrium of pregnant uterus and finally it was observed in the basal layer of placenta, but it disappeared rapidly in the myometrium and endometrium. The incorporation of 14C-leucine into fetal liver protein was decreased at the 20th day of gestation in the inoculated groups. The 14C-alanine transference to fetus across the placenta, gradually decreased at the 18th, 20th day of gestation in the inoculated groups. From these results we suspected the viral invasion ascending from vagina to placenta was occurred, then the IUGR was caused by damage of placenta function.</p>","PeriodicalId":75398,"journal":{"name":"Acta obstetrica et gynaecologica Japonica","volume":"33 5","pages":"681-9"},"PeriodicalIF":0.0,"publicationDate":"1981-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18248895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}