[Studies on the mechanism and the significance of prostaglandin biosynthesis by the ovary--conversion of 14C-arachidonic acid to prostaglandins (author's transl)].

Abstract

Biosynthesis of prostaglandins (PGs) from 14C-arachidonic acid was studied using homogenates of the ovaries from rats, gilts and human. Among the radioactive metabolites extracted, PGE2 and 6 keto PGF1 alpha were purified and identified by silicic acid column-, thin layers-, reversed phase partition chromatographies, and radiogaschromatography. Treatment with PMS-hCG activated metabolism of arachidonic acid into radioactive metabolites including PGE2 and 6 keto PGF1 alpha to a large extent. Therefore it is concluded that LH or (hCG) activate the PG synthetase system through the induction of synthetase itself and/or a stimulatory factor.