Acta microbiologica Academiae Scientiarum Hungaricae最新文献

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The stability of enterotoxin production in Yersinia enterocolitica and the methanol solubility of heat-stable enterotoxin. 小肠结肠炎耶尔森菌产肠毒素的稳定性及热稳定型肠毒素的甲醇溶解度。
D Velin, L Emödy
{"title":"The stability of enterotoxin production in Yersinia enterocolitica and the methanol solubility of heat-stable enterotoxin.","authors":"D Velin,&nbsp;L Emödy","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Twenty five strains of Yersinia enterocolitica serogroup O3, were isolated from human enteritis and studied for heat-stable enterotoxin production. Enterotoxin production was found even in the crude supernatant fluid of cultures that had been stored in stock agar for a year. According to the suckling mice and rabbit gut loop tests, after 1 to 5 years storage the filtrate showed heat-stable enterotoxin activity only in a purified and concentrated form. Following more than 5 years storage positive results could be obtained only in rabbit gut loop test. After 9 years the freeze dried strains still showed a full capacity of heat-stable enterotoxin production. Studies with concentrated substances showed that even after more than 9 years, there was no spontaneous loss of heat-stable enterotoxin production, only quantitative changes occurred. The methanol solubility of the heat-stable enterotoxin of Y. enterocolitica is--as distinct from the heat-stable enterotoxin of Escherichia coli--homogeneous and only the methanol soluble fractions showed any activity. The activity of methanol soluble enterotoxin from several years old subcultures could be demonstrated in an isolated rabbit gut loop model even when it failed to show any activity in suckling mice.</p>","PeriodicalId":75387,"journal":{"name":"Acta microbiologica Academiae Scientiarum Hungaricae","volume":"29 4","pages":"227-33"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17817409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Campylobacter jejuni enteritis: incidence, age distribution and clinical symptoms. 空肠弯曲杆菌性肠炎的发病率、年龄分布及临床症状。
M Kálmán, E Nagy, I Kiss, M Horváth
{"title":"Campylobacter jejuni enteritis: incidence, age distribution and clinical symptoms.","authors":"M Kálmán,&nbsp;E Nagy,&nbsp;I Kiss,&nbsp;M Horváth","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75387,"journal":{"name":"Acta microbiologica Academiae Scientiarum Hungaricae","volume":"29 3","pages":"217-9"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18183721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of endotoxin and radio-detoxified endotoxin on cell membranes in vitro. 内毒素和放射线解毒内毒素对体外细胞膜的影响。
T Kubasova, Z Somosy, L Bertók, G J Köteles
{"title":"Effect of endotoxin and radio-detoxified endotoxin on cell membranes in vitro.","authors":"T Kubasova,&nbsp;Z Somosy,&nbsp;L Bertók,&nbsp;G J Köteles","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The effect of parent lipopolysaccharide (LPS) and radio-detoxified endotoxin (rdLPS) on various human blood cell membranes proved to be different as detected by 3H-concanavalin A-binding technique in vitro. The lectin-binding ability of erythrocyte membranes did not change upon treatment by either endotoxin, whereas that of lymphocytes was stimulated by LPS at 10-50 micrograms/ml concentration as well as by rdLPS at the lowest dose applied, i.e. 0.1 micrograms/ml. The LPS-treated platelets bound 3H-concanavalin A less than did the untreated controls; on the other hand, the rdLPS did not change the lectin-binding surface of these cells. The affection by radiation of cell membranes could be prevented by pretreatment with endotoxins. This fact, however, could not be considered a radioprotective effect. The micromorphological investigations by scanning electron microscopy (SEM) support our data concerning the functional alterations of plasma membranes of platelets and lymphocytes after LPS and rdLPS treatment as well as after the combined effect of endotoxin pretreatment and X-irradiation, since a severe smoothening of the cell surface could be observed.</p>","PeriodicalId":75387,"journal":{"name":"Acta microbiologica Academiae Scientiarum Hungaricae","volume":"29 4","pages":"235-43"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17817410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Amikacin in experimental tuberculosis of guinea pigs. 阿米卡星在豚鼠实验性肺结核中的作用。
T Fodor
{"title":"Amikacin in experimental tuberculosis of guinea pigs.","authors":"T Fodor","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75387,"journal":{"name":"Acta microbiologica Academiae Scientiarum Hungaricae","volume":"29 2","pages":"137-8"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18153181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Higher resistance of germfree mice to dianhydrodulcitol, a lymphotropic cytostatic agent. 无菌小鼠对嗜淋巴细胞抑制剂二水杨醇的更高耐药性。
P Anderlik, I Szeri, Z Bános, M Wessely, B Radnai
{"title":"Higher resistance of germfree mice to dianhydrodulcitol, a lymphotropic cytostatic agent.","authors":"P Anderlik,&nbsp;I Szeri,&nbsp;Z Bános,&nbsp;M Wessely,&nbsp;B Radnai","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The same dose of dianhydrodulcitol (DAD) produced a lower mortality rate among germfree mice than among SPF or conventional C3H mice. On the other hand, it caused graver lymphoid atrophy in germfree mice. Their higher resistance, as evidenced by the mortality rate, can be explained on the basis of a histological study of the ileum. It showed milder alterations of the intestinal wall in germfree than in SPF mice. The lymphotropic cytostatic agent had a less direct toxic effect in germfree mice, due to the lacking damaging effect of endotoxin from the normal intestinal flora.</p>","PeriodicalId":75387,"journal":{"name":"Acta microbiologica Academiae Scientiarum Hungaricae","volume":"29 1","pages":"33-40"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17275062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cytotoxic material released from Staphylococcus epidermidis. II. Fractionation and some effects of the fractions on lymphocytes and hepatocytes. 表皮葡萄球菌释放的细胞毒性物质。2分离及其对淋巴细胞和肝细胞的影响。
M Solymossy, J Mandl, Z Nagy, F Antoni
{"title":"Cytotoxic material released from Staphylococcus epidermidis. II. Fractionation and some effects of the fractions on lymphocytes and hepatocytes.","authors":"M Solymossy,&nbsp;J Mandl,&nbsp;Z Nagy,&nbsp;F Antoni","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Cytotoxic substance(s) of about 4 X 10(3) molecular weight, containing 9.5% peptide and 73% carbohydrate was released from Staphylococcus epidermidis in phosphate buffered saline. The material was soluble in ethanol and was heat-resistant. It blocked amino acid uptake and E-rosette formation of human tonsillar and blood lymphocytes. In isolated mouse hepatocytes the toxin inhibited protein synthesis, but only in the presence of calcium ions. The results suggest that eukaryotic cell membranes are damaged by the coccal agent.</p>","PeriodicalId":75387,"journal":{"name":"Acta microbiologica Academiae Scientiarum Hungaricae","volume":"29 4","pages":"277-84"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18201519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Immune regulatory factors in sheep fetuses. 羊胎儿的免疫调节因子。
S Tuboly
{"title":"Immune regulatory factors in sheep fetuses.","authors":"S Tuboly","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>After an antigenic stimulus in vitro, a fraction was found in the supernatant fluids of spleen (S-1) and thymus (T-1) cell cultures of 70 to 80-day-old sheep fetuses infected with ovine adenovirus in utero. The fraction agreed with alpha-1-fetoprotein in mobility and its molecular weight was 67 000. Injected into adult sheep, S-1 and T-1 had a suppressive effect on the humoral immune response to both adenovirus and Clostridium perfringens D vaccine. Tests with peripheral lymphocytes suggested that fetal cell supernatants do not affect blastogenesis but markedly inhibit Ig synthesis as it could be demonstrated by immunofluorescence tests.</p>","PeriodicalId":75387,"journal":{"name":"Acta microbiologica Academiae Scientiarum Hungaricae","volume":"29 1","pages":"9-16"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17345567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Klebsiella pneumoniae enterotoxin. III. Effect of Klebsiella pneumoniae enterotoxin on the intestinal transport and histopathological changes in rabbit ileal loop. 肺炎克雷伯氏菌肠毒素。3肺炎克雷伯菌肠毒素对家兔回肠袢肠运输及组织病理学改变的影响。
P J Asnani, A Jhanjee
{"title":"Klebsiella pneumoniae enterotoxin. III. Effect of Klebsiella pneumoniae enterotoxin on the intestinal transport and histopathological changes in rabbit ileal loop.","authors":"P J Asnani,&nbsp;A Jhanjee","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Klebsiella pneumoniae strain B-5-1 was used to study the effect of its enterotoxin on the intestinal transport and histopathological changes in the rabbit ileal loop. Enterotoxin increased the net flux of water and of electrolytes. Increased amounts of phospholipids and proteins were also detected together with structural and functional abnormalities in the ileum. All these suggest that K. pneumoniae enterotoxin not only induce a net flux of fluid and imbalance of electrolytes but also damages the intestinal structure.</p>","PeriodicalId":75387,"journal":{"name":"Acta microbiologica Academiae Scientiarum Hungaricae","volume":"29 3","pages":"147-54"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17815633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Protective role of O antigen in Salmonella typhi-murium infection. O抗原对伤寒沙门氏菌感染的保护作用。
I Jaszovszky
{"title":"Protective role of O antigen in Salmonella typhi-murium infection.","authors":"I Jaszovszky","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Mice were actively immunized with preparations produced from different Salmonella strains. They were challenged with 2 LD50 of a virulent S. typhi-murium strain and examined for viable cell counts in the liver 4, 7 and 11 days postinfection. Whole cell vaccines, ribosomal extracts and endotoxin preparations of the O antigen-deficient variant of S. typhi-murium strain LT2-MI failed to protect the mice or did so in a much lower degree than preparations of the corresponding O antigen-bearing variant. Preparations from other salmonellae exerted a protective action only if the strain had an O-antigen identical with that of S. typhi-murium. The results pointed to a considerable protective role of the O antigen.</p>","PeriodicalId":75387,"journal":{"name":"Acta microbiologica Academiae Scientiarum Hungaricae","volume":"29 2","pages":"105-13"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17247838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Epidemic pulmonary infection associated with Mycobacterium xenopi indigenous in sewage-sludge. 流行性肺部感染与污水污泥中特有的异种分枝杆菌有关。
I Szabó, K K Kiss, I Várnai
{"title":"Epidemic pulmonary infection associated with Mycobacterium xenopi indigenous in sewage-sludge.","authors":"I Szabó,&nbsp;K K Kiss,&nbsp;I Várnai","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Mycobacterium xenopi was isolated from the sputum of 21 patients with clinical signs of pulmonary disease and of 52 asymptomatic subjects living in the environment of a sludge pool. M. xenopi was cultured in high numbers from sludge samples. The infections were assumed to occur partly via dry sludge particles scattered by the wind in summer, and partly by sludge used as fertilizer.</p>","PeriodicalId":75387,"journal":{"name":"Acta microbiologica Academiae Scientiarum Hungaricae","volume":"29 4","pages":"263-6"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17370333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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