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Acta microbiologica Academiae Scientiarum Hungaricae最新文献

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Vital fluorescence microscopy of lysosomes in cultured mouse peritoneal macrophages during their interactions with microorganisms and active substances. III. Interactions of macrophages with endozoits of Toxoplasma gondii RH strain and their soluble substance. 培养的小鼠腹膜巨噬细胞与微生物和活性物质相互作用时溶酶体的荧光显微镜。3刚地弓形虫RH株巨噬细胞与内殖体及其可溶性物质的相互作用。
Acta microbiologica Academiae Scientiarum Hungaricae Pub Date : 1980-01-01
T N Khavkin, I S Freidlin, A K Shustrov
{"title":"Vital fluorescence microscopy of lysosomes in cultured mouse peritoneal macrophages during their interactions with microorganisms and active substances. III. Interactions of macrophages with endozoits of Toxoplasma gondii RH strain and their soluble substance.","authors":"T N Khavkin,&nbsp;I S Freidlin,&nbsp;A K Shustrov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Macrophages with lysosomes pinpointed by quinacrine-induced fluorescence were infected with the endozoits of Toxoplasma gondii RH strain (peritoneal exudate of infected mouse), or treated with liquid (acellular) fraction of the same exudate. Dead toxoplasmas ingested by macrophages come into contact with the stained lysosomes of the cell and acquire a diffuse fluorescence. Viable toxoplasmas do not give fluorescence, which means that they do not come into contact with lysosomes, either primary or secondary. This supports the hypothesis that toxoplasmas can prevent lysosomes from fusing with the phagosomes of the host cell. Moderate doses of soluble products of toxoplasmas contained in peritoneal exudate cause an excessive output of macrophage lysosomes which points to the activation of macrophages; high doses of challenge inhibit the phagocytosis of toxoplasmas and damage macrophages. The pathogenicity of toxoplasmas due to their ability to inhibit the fusion of lysosomes and phagosomes and the cellular action of their soluble products is discussed.</p>","PeriodicalId":75387,"journal":{"name":"Acta microbiologica Academiae Scientiarum Hungaricae","volume":"27 1","pages":"9-21"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18426842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Production and antigenic effect of experimental sheep adenovirus vaccines. 绵羊腺病毒实验疫苗的制备及其抗原效应。
Acta microbiologica Academiae Scientiarum Hungaricae Pub Date : 1980-01-01
V Pálfi, S Belák
{"title":"Production and antigenic effect of experimental sheep adenovirus vaccines.","authors":"V Pálfi,&nbsp;S Belák","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Adenovirus vaccines inactivated and adsorbed with different methods were prepared for the specific control of pneumoenteritis of lambs. Beta-propiolactone and formalin as inactivating agents and aluminium hydroxide gel and DEAE dextran as adjuvants were compared. Vaccines inactivated and adsorbed in different ways provoked practically the same immunological response. Seronegative lambs developed a higher level of antibodies after the administration of monovalent vaccines, than in the case of a bivalent one. It is concluded that inactivated and adsorbed adenovirus vaccines provoke a significant production of antibodies in sheep.</p>","PeriodicalId":75387,"journal":{"name":"Acta microbiologica Academiae Scientiarum Hungaricae","volume":"27 2","pages":"135-9"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18426844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Susceptibility of birds to type-B influenza virus. 鸟类对b型流感病毒的易感性。
Acta microbiologica Academiae Scientiarum Hungaricae Pub Date : 1980-01-01
J Romváry, J Mészáros, K Barb
{"title":"Susceptibility of birds to type-B influenza virus.","authors":"J Romváry,&nbsp;J Mészáros,&nbsp;K Barb","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Among Zoo birds and migrating wild birds examined serologically during an outbreak due to influenza B virus, 4.1% were found to have serum antibodies to the agent. Evidence of avian susceptibility to the agent was emerging from reisolations of the virus and primary and secondary HI antibody response of the birds in subsequent infection experiments performed on adult pheasants and mallards, as well as one-day-old domestic ducklings and chicks. The virus persisted in the pheasants for at least three weeks, and adult pheasants, mallards and domestic ducklings were found to transmit it by contact to part of their susceptible mates.</p>","PeriodicalId":75387,"journal":{"name":"Acta microbiologica Academiae Scientiarum Hungaricae","volume":"27 4","pages":"279-87"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17321072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The procoagulant activity of leukocytes pretreated with radiodetoxified endotoxin. 放射性解毒内毒素预处理白细胞的促凝活性。
Acta microbiologica Academiae Scientiarum Hungaricae Pub Date : 1980-01-01
T Szilágyi, H Csernyánszky, E Gazdy, L Bertók
{"title":"The procoagulant activity of leukocytes pretreated with radiodetoxified endotoxin.","authors":"T Szilágyi,&nbsp;H Csernyánszky,&nbsp;E Gazdy,&nbsp;L Bertók","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Rabbits were treated with Escherichia coli 089 endotoxin detoxified by ionizing irradiation (60 Co-gamma). The leukocytes (PMNs in 90%) obtained from rabbits that had been treated with the mother endotoxin elicited a well defined, those obtained from rabbits pretreated with detoxified endotoxin elicited a less pronounced, procoagulant activity. It is suggested that the procoagulant effect may play a part in the mechanism of the local Shwartzman phenomenon.</p>","PeriodicalId":75387,"journal":{"name":"Acta microbiologica Academiae Scientiarum Hungaricae","volume":"27 3","pages":"191-4"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18052801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization of conidiation mutants in Trichoderma viride by hyphal anastomosis and protoplast fusion. 利用菌丝吻合和原生质体融合对绿木霉分生突变体的鉴定。
Acta microbiologica Academiae Scientiarum Hungaricae Pub Date : 1980-01-01
A Bojnanská, M Sipiczki, L Ferenczy
{"title":"Characterization of conidiation mutants in Trichoderma viride by hyphal anastomosis and protoplast fusion.","authors":"A Bojnanská,&nbsp;M Sipiczki,&nbsp;L Ferenczy","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Conidiation mutants were isolated from the fungus Trichoderma viride, and tested for complementation by both anastomosis and protoplast fusion. They could be grouped into three classes: (1) colour mutants; (2) mutants with reduced conidiation; and (3) nonconidiating mutants. Several mutants of classes (2) and (3) were incapable of anastomosis, but via protoplast fusion they produced heterokaryons suitable for complementation tests.</p>","PeriodicalId":75387,"journal":{"name":"Acta microbiologica Academiae Scientiarum Hungaricae","volume":"27 4","pages":"305-7"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18207974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enterotoxin production of Yersinia enterocolitica strains. 小肠结肠炎耶尔森菌菌株肠毒素的产生。
Acta microbiologica Academiae Scientiarum Hungaricae Pub Date : 1980-01-01
D Velin, L Emödy, S Pácsa, T Kontrohr
{"title":"Enterotoxin production of Yersinia enterocolitica strains.","authors":"D Velin,&nbsp;L Emödy,&nbsp;S Pácsa,&nbsp;T Kontrohr","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Eighteen Yersinia enterocolitica serogroup O3, rhamnose negative strains isolated in Hungary from human enteritis, have been studied for enterotoxin production. Freshly isolated strains cultivated at 25 degrees C produced heat stable (ST) enterotoxin demonstrable in sucking mice, whereas strains isolated earlier and maintained in subculture lost their toxin producing capacity. No heat labile enterotoxin (LT) was found in the filtrate of the cultures or in their sonicated lysate. The ST of one strain exerted a dilating effect on the intestinal loop of rabbits. Strains cultured at 37 degrees C produced no toxin. Toxin production was demonstrable in Syncase medium but not in the modified Sakazaki medium. None of the strains caused keratoconjunctivitis; three strains elicited mild conjunctivitis in guinea pigs.</p>","PeriodicalId":75387,"journal":{"name":"Acta microbiologica Academiae Scientiarum Hungaricae","volume":"27 4","pages":"299-304"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18475302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Salmonella typhi R-plasmids in Hungary. 匈牙利伤寒沙门氏菌r质粒。
Acta microbiologica Academiae Scientiarum Hungaricae Pub Date : 1980-01-01
V G László, H Milch
{"title":"Salmonella typhi R-plasmids in Hungary.","authors":"V G László,&nbsp;H Milch","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The antibiotic sensitivity of 4095 Salmonella typhi strains isolated in Hungary from January, 1974 to June 1979, was tested. Twelve strains derived from one patient and seven chronic carriers were resistant to antibiotics due to R-plasmids. One of the S. typhi strains carried two R-plasmids and the change in the phage type of this strain was caused by one of the plasmids. R-plasmid of the same restrictive property was found in an Escherichia coli strain isolated from the faeces of the corresponding carrier. All of the R-plasmids examined were fi-, E. coli and Shigella flexneri phages were restricted by four R-plasmids, S. typhi phages were restricted by one. R-plasmids belonged to incompatibility groups I1, I2, W and H.</p>","PeriodicalId":75387,"journal":{"name":"Acta microbiologica Academiae Scientiarum Hungaricae","volume":"27 4","pages":"325-32"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18475303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
New mechanism of plasmid curing by psychotropic drugs. 精神药物治疗质粒的新机制。
Acta microbiologica Academiae Scientiarum Hungaricae Pub Date : 1980-01-01
J Molnár, B Schneider, Y Mándi, S Farkas, I B Holland
{"title":"New mechanism of plasmid curing by psychotropic drugs.","authors":"J Molnár,&nbsp;B Schneider,&nbsp;Y Mándi,&nbsp;S Farkas,&nbsp;I B Holland","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Methylene blue enhanced the plasmid curing efficiency of chlorpromazine, imipramine and amitriptyline with strains of Escherichia coli K12 carrying F-prime lac or the resistance factor R-144. In contrast, methylene blue inhibited the elimination of plasmids by acridine orange and ethydium bromide at all concentrations tested. Two metabolic derivatives of chlorpromazine, chlorpromazine sulphoxide and 7.8-dioxochlorpromazine had no plasmid curing effect even in the presence of methylene blue. Amitriptyline, 7,8-dioxochlorpromazine and acridine orange were effective inhibitors of the conjugal transfer of the resistance plasmid, R-144, whilst methylene blue, chlorpromazine sulphoxide, and imipramine had only slight effects. We were therefore unable to demonstrate a simple correlation between curing ability and inhibition of plasmid transfer amongst the psychoactive drugs tested. A mechanism of plasmid curing by surface action of the drugs is suggested as an alternative to direct intercalation of the drugs into plasmid DNA.</p>","PeriodicalId":75387,"journal":{"name":"Acta microbiologica Academiae Scientiarum Hungaricae","volume":"27 4","pages":"309-15"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18057121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of methicillin on the phospholipid content of methicillin sensitive Staphylococcus aureus. 甲氧西林对甲氧西林敏感金黄色葡萄球菌磷脂含量的影响。
Acta microbiologica Academiae Scientiarum Hungaricae Pub Date : 1980-01-01
F Rozgonyi, J Kiss, P Jékel, L Váczi
{"title":"Effect of methicillin on the phospholipid content of methicillin sensitive Staphylococcus aureus.","authors":"F Rozgonyi,&nbsp;J Kiss,&nbsp;P Jékel,&nbsp;L Váczi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The effects of methicillin at concentrations of 1 microgram/ml (= 1/2 x minimum inhibitory concentration) and 2 microgram/ml (= 1 x MIC) on the phospholipid content of methicillin-sensitive Staphylococcus aureus 5814S growing exponentially were studied. During 2-hour treatment 1/2 x MIC of methicillin induced a significant increase in the phospholipid content of the cocci; accumulation of acid phospholipids was prevalent. In contrast, addition of 1 x MIC of methicillin to the cultures resulted in significantly less phospholipids in the cocci compared with that of the control cultures. There were some alterations in the amounts of individual phospholipids during treatment. The phosphatidic acid (PA) and diphosphatidylglycerol--cardiolipin (DPG) contents were significantly reduced in the first hour then reached the control values by the end of the 2nd hr of exposure. An opposite change was found in the amount of lysylphosphatidylglycerol (LPG), whereas the phosphatidylglycerol (PG) level was significantly lower in the treated cultures than in the controls of corresponding age. Phospholipid synthesis seems to be one of the sites of the action of methicillin in S. aureus.</p>","PeriodicalId":75387,"journal":{"name":"Acta microbiologica Academiae Scientiarum Hungaricae","volume":"27 1","pages":"31-40"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18423899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Difference in the virulence of Ureaplasma urealyticum isolates. 解脲支原体分离株的毒力差异。
Acta microbiologica Academiae Scientiarum Hungaricae Pub Date : 1980-01-01
M Fodor
{"title":"Difference in the virulence of Ureaplasma urealyticum isolates.","authors":"M Fodor","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The virulence of Ureaplasma urealyticum strains was tested by intrarenally infecting white mice of undefined, LATI (Gödöllö, Hungary) and BALB/c breed. Freshly isolated human strains differed in virulence; when subcultured in U-9 medium they showed a gradual loss of virulence. Ureaplasma strains of calf and swine origin were virulent to mice in a medium degree. Some of the inoculated mice showed the signs of latent infection.</p>","PeriodicalId":75387,"journal":{"name":"Acta microbiologica Academiae Scientiarum Hungaricae","volume":"27 2","pages":"161-9"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18426845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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