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Acta microbiologica Academiae Scientiarum Hungaricae最新文献

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New mechanism of plasmid curing by psychotropic drugs. 精神药物治疗质粒的新机制。
Acta microbiologica Academiae Scientiarum Hungaricae Pub Date : 1980-01-01
J Molnár, B Schneider, Y Mándi, S Farkas, I B Holland
{"title":"New mechanism of plasmid curing by psychotropic drugs.","authors":"J Molnár,&nbsp;B Schneider,&nbsp;Y Mándi,&nbsp;S Farkas,&nbsp;I B Holland","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Methylene blue enhanced the plasmid curing efficiency of chlorpromazine, imipramine and amitriptyline with strains of Escherichia coli K12 carrying F-prime lac or the resistance factor R-144. In contrast, methylene blue inhibited the elimination of plasmids by acridine orange and ethydium bromide at all concentrations tested. Two metabolic derivatives of chlorpromazine, chlorpromazine sulphoxide and 7.8-dioxochlorpromazine had no plasmid curing effect even in the presence of methylene blue. Amitriptyline, 7,8-dioxochlorpromazine and acridine orange were effective inhibitors of the conjugal transfer of the resistance plasmid, R-144, whilst methylene blue, chlorpromazine sulphoxide, and imipramine had only slight effects. We were therefore unable to demonstrate a simple correlation between curing ability and inhibition of plasmid transfer amongst the psychoactive drugs tested. A mechanism of plasmid curing by surface action of the drugs is suggested as an alternative to direct intercalation of the drugs into plasmid DNA.</p>","PeriodicalId":75387,"journal":{"name":"Acta microbiologica Academiae Scientiarum Hungaricae","volume":"27 4","pages":"309-15"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18057121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Lysosomal acridine orange uptake in fibroblasts transformed by SV40 or human cytomegalovirus. SV40或人巨细胞病毒转化成纤维细胞对溶酶体吖啶橙的摄取。
Acta microbiologica Academiae Scientiarum Hungaricae Pub Date : 1980-01-01
I Rédai, M Halmy
{"title":"Lysosomal acridine orange uptake in fibroblasts transformed by SV40 or human cytomegalovirus.","authors":"I Rédai,&nbsp;M Halmy","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Lysosomes of living human fibroblasts, SV40-transformed rat fibroblasts and human CMV-transformed hamster fibroblasts were examined by fluorescence microscopy after pretreatment with acridine orange at a supravital concentrations (5 x 10(6) M). Dye uptake by human primary fibroblast lysosomes was considerable and independent of the age of the cultures. In the transformed cultures, cytoplasmic granular red fluorescence indicating lysosomal acridine orange uptake could not be observed in part of the cells; cells showing no cytoplasmic granular fluorescence appeared as early as after 48 hr incubation and were growing in dependence on the age of the culture. Staining of living cells by acridine orange solutions at supravital concentration is a practicable method for the examination of functional changes of lysosomes.</p>","PeriodicalId":75387,"journal":{"name":"Acta microbiologica Academiae Scientiarum Hungaricae","volume":"27 1","pages":"41-5"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17314459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of methicillin on the phospholipid content of methicillin sensitive Staphylococcus aureus. 甲氧西林对甲氧西林敏感金黄色葡萄球菌磷脂含量的影响。
Acta microbiologica Academiae Scientiarum Hungaricae Pub Date : 1980-01-01
F Rozgonyi, J Kiss, P Jékel, L Váczi
{"title":"Effect of methicillin on the phospholipid content of methicillin sensitive Staphylococcus aureus.","authors":"F Rozgonyi,&nbsp;J Kiss,&nbsp;P Jékel,&nbsp;L Váczi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The effects of methicillin at concentrations of 1 microgram/ml (= 1/2 x minimum inhibitory concentration) and 2 microgram/ml (= 1 x MIC) on the phospholipid content of methicillin-sensitive Staphylococcus aureus 5814S growing exponentially were studied. During 2-hour treatment 1/2 x MIC of methicillin induced a significant increase in the phospholipid content of the cocci; accumulation of acid phospholipids was prevalent. In contrast, addition of 1 x MIC of methicillin to the cultures resulted in significantly less phospholipids in the cocci compared with that of the control cultures. There were some alterations in the amounts of individual phospholipids during treatment. The phosphatidic acid (PA) and diphosphatidylglycerol--cardiolipin (DPG) contents were significantly reduced in the first hour then reached the control values by the end of the 2nd hr of exposure. An opposite change was found in the amount of lysylphosphatidylglycerol (LPG), whereas the phosphatidylglycerol (PG) level was significantly lower in the treated cultures than in the controls of corresponding age. Phospholipid synthesis seems to be one of the sites of the action of methicillin in S. aureus.</p>","PeriodicalId":75387,"journal":{"name":"Acta microbiologica Academiae Scientiarum Hungaricae","volume":"27 1","pages":"31-40"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18423899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Difference in the virulence of Ureaplasma urealyticum isolates. 解脲支原体分离株的毒力差异。
Acta microbiologica Academiae Scientiarum Hungaricae Pub Date : 1980-01-01
M Fodor
{"title":"Difference in the virulence of Ureaplasma urealyticum isolates.","authors":"M Fodor","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The virulence of Ureaplasma urealyticum strains was tested by intrarenally infecting white mice of undefined, LATI (Gödöllö, Hungary) and BALB/c breed. Freshly isolated human strains differed in virulence; when subcultured in U-9 medium they showed a gradual loss of virulence. Ureaplasma strains of calf and swine origin were virulent to mice in a medium degree. Some of the inoculated mice showed the signs of latent infection.</p>","PeriodicalId":75387,"journal":{"name":"Acta microbiologica Academiae Scientiarum Hungaricae","volume":"27 2","pages":"161-9"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18426845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Membrane characteristics of the hemA mutant of Bacillus subtilis. 枯草芽孢杆菌hemA突变体的膜特性。
Acta microbiologica Academiae Scientiarum Hungaricae Pub Date : 1980-01-01
A Miczák, B Prágai, B Taródi
{"title":"Membrane characteristics of the hemA mutant of Bacillus subtilis.","authors":"A Miczák,&nbsp;B Prágai,&nbsp;B Taródi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The membrane of the delta-aminolaevulinic acid synthase (EC. 2.3.1.37) deficient mutant of Bacillus subtilis growing in the presence of delta-aminolaevulinic acid differs only to a limited extent from the wild type. In haemin-containing medium, however, significant differences are observed as regards the osmotic stability of the protoplasts and the membrane protein composition.</p>","PeriodicalId":75387,"journal":{"name":"Acta microbiologica Academiae Scientiarum Hungaricae","volume":"27 1","pages":"99-102"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17827632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In vitro transfer of multiple resistance observed in vivo during a Salmonella london epidemic. 伦敦沙门氏菌流行期间观察到体内多重耐药性的体外转移。
Acta microbiologica Academiae Scientiarum Hungaricae Pub Date : 1980-01-01
J Lantos, E Marjai
{"title":"In vitro transfer of multiple resistance observed in vivo during a Salmonella london epidemic.","authors":"J Lantos,&nbsp;E Marjai","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Between 1976 and 1978, waves of Salmonella london infections conveyed by raw meat and meat products were observed. The strains isolated during the epidemic were first susceptible then developed multiple antibiotic resistance. The identical antibiotic resistance patterns of the strain and their more frequent occurrence in hospital environments indicated plasmid-mediated resistance. R-plasmid transfer, minimum inhibition concentration and resistance elimination were studied in representative strains. The resistant S. london strain and transconjugants of Escherichia coli rendered resistant were compared. The results proved that multiple resistance was plasmid-mediated.</p>","PeriodicalId":75387,"journal":{"name":"Acta microbiologica Academiae Scientiarum Hungaricae","volume":"27 1","pages":"47-53"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18426836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Lack of correlation between intercalation and plasmid curing ability of some tricyclic compounds. 一些三环化合物的嵌入层与质粒固化能力之间缺乏相关性。
Acta microbiologica Academiae Scientiarum Hungaricae Pub Date : 1980-01-01
K Barabás, J Molnár
{"title":"Lack of correlation between intercalation and plasmid curing ability of some tricyclic compounds.","authors":"K Barabás,&nbsp;J Molnár","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Some tricyclic psychotropic drugs are known to have plasmid curing activity. The interaction with DNA of three plasmid curing (chlorpromazine, amitriptyline, imipramine) and four ineffective (methylene blue, 7,8-dioxo-chlorpromazine, thiazinamium, chlorpromazine sulphoxide) compounds was studied by fluorescence polarization and circular dichroism methods. Among the seven compounds three, namely chlorpromazine, 7,8-O2-chlorpromazine, and methylene blue showed an intercalation effect. Other phenothiazines such as chlorpromazine sulphoxide and thiazinamium were not able to intercalate into Escherichia coli DNA, neither did the plasmid curing drugs amitryptyline and imipramine. It is concluded that the plasmid curing ability is not necessarily related to the intercalation ability.</p>","PeriodicalId":75387,"journal":{"name":"Acta microbiologica Academiae Scientiarum Hungaricae","volume":"27 1","pages":"55-61"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17173516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Specific antibodies to viruses HL-23 and BILN in the blood plasma of patients with acute myelogenous leukaemia and with potential preleukaemia. 急性髓性白血病和潜在白血病前期患者血浆中HL-23和BILN病毒特异性抗体的研究
Acta microbiologica Academiae Scientiarum Hungaricae Pub Date : 1980-01-01
F D Tóth, J Kiss, L Váczi, Z Madár, J Jakó, K Rák
{"title":"Specific antibodies to viruses HL-23 and BILN in the blood plasma of patients with acute myelogenous leukaemia and with potential preleukaemia.","authors":"F D Tóth,&nbsp;J Kiss,&nbsp;L Váczi,&nbsp;Z Madár,&nbsp;J Jakó,&nbsp;K Rák","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Blood plasma samples from patients with acute myelogenous leukaemia (AML) or potential preleukaemia and from control subjects were tested for antibodies to the viruses HL-23 and BILN by membrane immunofluorescence. Of 15 patients with untreated AML, three, each having a low peripheral leucocyte count at the time of sampling, had detectable antibodies. Antibodies were present in the plasma of 5 out of 8 AML patients being in remission as a result of chemotherapy. In these cases, the antibody levels significantly exceeded those demonstrated in the untreated cases. Of 12 patients with potential preleukaemia, five proved to be positive. Of the 7 antibody-negative patients, four developed manifest leukaemia within 12-18 months after the first testing. The results are suggestive of a favourable prognostic role of the presence of the antibodies under study. In the majority of the antibody-positive AML and potential preleukameia cases antibodies were detectable to both components of the HL-23 virus. Of 30 control subjects, three had demonstrable antibodies to the BILN virus.</p>","PeriodicalId":75387,"journal":{"name":"Acta microbiologica Academiae Scientiarum Hungaricae","volume":"27 2","pages":"147-53"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17314460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
New selective medium for the isolation of Haemophilus species. 分离嗜血杆菌种的新选择培养基。
Acta microbiologica Academiae Scientiarum Hungaricae Pub Date : 1980-01-01
Z Csukás
{"title":"New selective medium for the isolation of Haemophilus species.","authors":"Z Csukás","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Chocolate agar containing lincomycin (5 micrograms/ml) was used for the isolation of haemophilus strains. One-hundred haemophilus strains of 3 species grew well on selective plates, whereas Gram-positive bacterial failed to form colonies on them. The selective culture proved especially advantageous when throat swabs were examined.</p>","PeriodicalId":75387,"journal":{"name":"Acta microbiologica Academiae Scientiarum Hungaricae","volume":"27 2","pages":"141-5"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18015831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
alpha-Haemolysin: an additive virulence factor in Escherichia coli. α -溶血素:大肠杆菌的附加毒力因子。
Acta microbiologica Academiae Scientiarum Hungaricae Pub Date : 1980-01-01
L Emödy, T Pál, N V Safonova, B Kuch, N K Golutva
{"title":"alpha-Haemolysin: an additive virulence factor in Escherichia coli.","authors":"L Emödy,&nbsp;T Pál,&nbsp;N V Safonova,&nbsp;B Kuch,&nbsp;N K Golutva","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Haemolytic Escherichia coli, including human intestinal and extraintestinal as well as porcine enterotoxigenic and oedema disease isolates, and Proteus morganii strains were studied for their virulence. Hly+ wild type strains and Hly+ transconjugants were more virulent than Hly- derivatives as shown in mice and chick embryos. This enhanced virulence seems to be connected with the ability of diffusible alpha-haemolysin production because clones producing only non-diffusible, beta-haemolysin behaved as non-haemolytic ones. Haemorrhagic lung symptoms and haemoglobinuria were frequently observed after parenteral challenge of mice with alpha-haemolytic clones. Though the Hly- clone exhibited a high resistance against blood clearance, the number of circulating bacteria was significantly higher in the case of alpha-haemolytic clone. A causal connection between this phenomenon and the leukocidin activity of alpha-haemolysin is suggested.</p>","PeriodicalId":75387,"journal":{"name":"Acta microbiologica Academiae Scientiarum Hungaricae","volume":"27 4","pages":"333-42"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18057123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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