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Production, Purification and Characterization of Cellulase from Cossus cossus Larvae Cossus幼虫纤维素酶的生产、纯化及性质研究
Al-Kimia Pub Date : 2022-06-27 DOI: 10.24252/al-kimia.v10i1.28071
M. Baharuddin
{"title":"Production, Purification and Characterization of Cellulase from Cossus cossus Larvae","authors":"M. Baharuddin","doi":"10.24252/al-kimia.v10i1.28071","DOIUrl":"https://doi.org/10.24252/al-kimia.v10i1.28071","url":null,"abstract":"This study aimed to purify and characterize the biochemical properties of cellulase from bacterial isolates of C. cossus larvae, to determine the effect of adding crude extract cellulase and pure enzymes to the hydrolysis of rice straw. The research steps included purification of enzymes by ion exchange chromatography and gel filtration chromotography, as well as SDS-PAGE electrophoresis analysis. Pure cellulases are characterized by their biochemical properties and applied in the hydrolysis of cellulose. The results showed that the characteristics of an optimum pH of 5.6, an optimum temperature of 70⁰C  and a substrate concentration of 1.5% CMC, and a molecular weight of 36.49 kDa. \u0000 ","PeriodicalId":7535,"journal":{"name":"Al-Kimia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49374256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enkapsulasi Asap Cair Tandan Kosong Kelapa Sawit (Elaeis guineensis Jacq.) dan Uji Aktivitas Antijamur 对棕榈油空棕榈油液体烟的熏蒸(Jacq的几尼二烯酸)和抗真菌活性测试
Al-Kimia Pub Date : 2022-06-25 DOI: 10.24252/al-kimia.v10i1.25723
Riskawati, Nurul Magfira, Maswati Baharuddin, Sappewali, F. Azis
{"title":"Enkapsulasi Asap Cair Tandan Kosong Kelapa Sawit (Elaeis guineensis Jacq.) dan Uji Aktivitas Antijamur","authors":"Riskawati, Nurul Magfira, Maswati Baharuddin, Sappewali, F. Azis","doi":"10.24252/al-kimia.v10i1.25723","DOIUrl":"https://doi.org/10.24252/al-kimia.v10i1.25723","url":null,"abstract":"Oil palm empty bunches is  lignocellulose that can be processed into liquid smoke with the content of acid compounds and phenol compounds that function as a natural preservative. The purpose of this study  to find out the characteristics of liquid smoke, see the effect of encapsulan on the liquid smoke encapsulation  process, and see the antifungal activity of A. flavus and A. niger. The method used is pyrolysis process at a temperature of 400 oC to produce liquid smoke and encapsulation  process by using variation  of coating material using spray dryer with micro sized result. The result showed that the liquid smoke characteristics can be seen from its chemical content that is the dominant phenol compound and has the highest phenol content among the parts found in oil palm that is 68,15%. Based on analysis it can be seen that the best encapsulation process is by the addition of chitosan on maltodextrin with water content 5.78%, phenol content of 43.03 ppm and has a high rate of encapsulation efficiency of 45.87%. The liquid smoke microcapsules could inhibit the growth of fungi with the highest value of liquid smoke concentration, that is 3% with inhibitory value of 8.30 mm.","PeriodicalId":7535,"journal":{"name":"Al-Kimia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47245075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identifikasi Komponen Kimia Ekstrak Daun Ketapang (Terminalia cattapa) Berdasarkan Perbandingan Metode Ekstraksi 根据提取方法的比较,确定提取物的化学成分
Al-Kimia Pub Date : 2022-06-25 DOI: 10.24252/al-kimia.v10i1.25457
Ismail Marzuki, Melisa Mirsyah, Selfina Gala
{"title":"Identifikasi Komponen Kimia Ekstrak Daun Ketapang (Terminalia cattapa) Berdasarkan Perbandingan Metode Ekstraksi","authors":"Ismail Marzuki, Melisa Mirsyah, Selfina Gala","doi":"10.24252/al-kimia.v10i1.25457","DOIUrl":"https://doi.org/10.24252/al-kimia.v10i1.25457","url":null,"abstract":"Screening, characterization and identification of chemical components of typical Indonesian plants are important for the purpose of finding high potential chemical components in the use of primary sources of cosmetic, medicinal and food ingredients. The selection of the right extraction method and the appropriate type of solvent is very decisive in the acquisition of the target chemical components. The brand of this research focuses on the comparison of extraction between soxhletation and maceration methods in identifying the chemical components of Ketapang leaves (Terminalia Catappa). Sample preparation was carried out by selecting fresh, cleaned, dried and mashed Ketapang leaves. The extraction was carried out in two stages using two types of solvents, namely the first stage of extraction using ethanol. Obtained a dilute extract, then concentrated using a rotary evaporator. The second extraction used n-hexane as solvent. There are 4 kinds of extracts, each ethanol extract and n-hexane extract by applying the soxhletation and maceration method. The results obtained showed that: the application of the maceration method to the identification of chemical components of ketapang leaves gave relatively better separation results than the soxhletation method. The number of chemical components identified from the ethanol extract of ketapang leaves is ± 20 species which are dominated by fatty acid compounds. There are two groups of chemical compounds identified from ketapang leaves using n-hexane as solvent both in the application of soxhletation and maceration methods with high similarity 90%, namely 4 kinds of chemical components of the fatty acid group (tetradecanoic acid, Hexadecanoic, Octadecanoid, Octadeca-9,12 -Dienoite) and 2 kinds of aliphatic components (methyl cyclopentane, Tetraco Hexane)","PeriodicalId":7535,"journal":{"name":"Al-Kimia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47345027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In Vitro Anti-inflammatory Activity of Extract and Fraction Seed Coat Kebiul (Caesalpinia bonduc L.) 种皮提取物及部位体外抗炎活性的研究
Al-Kimia Pub Date : 2022-06-16 DOI: 10.24252/al-kimia.v10i1.23769
Raden Fatahillah, D. Fitriyani, F. Wijayanti
{"title":"In Vitro Anti-inflammatory Activity of Extract and Fraction Seed Coat Kebiul (Caesalpinia bonduc L.)","authors":"Raden Fatahillah, D. Fitriyani, F. Wijayanti","doi":"10.24252/al-kimia.v10i1.23769","DOIUrl":"https://doi.org/10.24252/al-kimia.v10i1.23769","url":null,"abstract":"Inflammation is the protective reaction of body and supporting element of tissues to injurious stimuli, such as physical injury, tissue and cell damage by pathogens, and protein denaturation. This study aims to compare the antiinflammatory activity of ethanol extract, ethanol fraction, and n-hexane fraction from kebiul (Caesalpinia bonduc L.) seed coat. Antiinflammatory activity was tested by measuring the % inhibition of the denatured protein using a UV-Vis spectrophotometer. From the result of the study obtained IC50 value 2,10 μg/mL, 7,89 μg/mL, 4,04 μg/mL & 11,03 μg/mL. \u0000  \u0000  \u0000 ","PeriodicalId":7535,"journal":{"name":"Al-Kimia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43837262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Modifikasi Lumpur PDAM-Alginat Sebagai Peng-Enkapsulasi Urea dan Aplikasinya Sebagai Pupuk Lepas Lambat PDAM-Alginat泥浆作为尿素分解和用于快速释放肥料的应用的改进
Al-Kimia Pub Date : 2022-06-07 DOI: 10.24252/al-kimia.v10i1.23740
Ismail Astar, Ida Ayu Suci
{"title":"Modifikasi Lumpur PDAM-Alginat Sebagai Peng-Enkapsulasi Urea dan Aplikasinya Sebagai Pupuk Lepas Lambat","authors":"Ismail Astar, Ida Ayu Suci","doi":"10.24252/al-kimia.v10i1.23740","DOIUrl":"https://doi.org/10.24252/al-kimia.v10i1.23740","url":null,"abstract":"Modification of sludge solid waste regional water company (PDAM) Pontianak with alginate for the application of slow-release urea fertilizer. Characterization of materials was performed with Fourier-Transform Infra-Red (FTIR) spectroscopy and scanning electron microscopy (SEM). Analysis of the FTIR spectrum obtained showed that the characteristics of the absorption band at wavelengths of 3639.89 cm-1 and 3690.9 cm-1 are characteristic of the vibration of NH2 stretching asymmetric and symmetric derived from the presence of urea. Nitrogen release tests on mud PDAM-alginate composites with concentrations of 10%, 20%, 30%, 40% and 50% for 72 consecutive hours of 12,80%, 19,33%, 4,00%, 8,39% and 6,20%. This shows that urea encapsulated mud PDAM-alginate at a concentration of 30% is the composition with the best results in holding urea encapsulated. \u0000 ","PeriodicalId":7535,"journal":{"name":"Al-Kimia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41701149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Penentuan Logam Cr(VI) Menggunakan Metoda Voltammetri Stripping Anoda (VSA) pada Sampel Air Laut dan Air Sungai 阳极溶出伏安法测定海水和河水中的Cr(VI)
Al-Kimia Pub Date : 2022-06-07 DOI: 10.24252/al-kimia.v10i1.21799
Hilfi Pardi, Nancy Willian
{"title":"Penentuan Logam Cr(VI) Menggunakan Metoda Voltammetri Stripping Anoda (VSA) pada Sampel Air Laut dan Air Sungai","authors":"Hilfi Pardi, Nancy Willian","doi":"10.24252/al-kimia.v10i1.21799","DOIUrl":"https://doi.org/10.24252/al-kimia.v10i1.21799","url":null,"abstract":"Penelitian dengan tujuan penentuan logam Cr(VI) dalam sampel air laut dan sungai menggunakan metoda Voltammetri Stripping Anoda (VSA) telah dilakukan dengan mencari kondisi optimum potensial deposisi, waktu deposisi, pH, konsentrasi elektrolit pengukuran. Serta melihat ketelitian dan ketepan metoda maka dilakukan penentuan nilai simpangan baku relatif (SRD) dan nilai perolehan kembali. Hasil yang diperoleh adalah kondisi potensial deposisi -1,3 V, waktu deposisi 60 detik, konsentrasi larutan elektrolit pendukung NH4Cl 0,2 M, pH 10, Nilai SDR adalah 2,36%, dan nilai persen recovery 99,91%. Sebagai aplikasi dari metoda ini dilakukan penentuan Cr(VI) dalam sampel air laut Senggarang, dan air sungai Carang Tanjungpinang. Diperoleh konsentrasi Cr(VI) dalam sampel air laut Senggarang 133,751 µg/L, dan dalam sampel air Sungai Carang 34,702 µg/L.","PeriodicalId":7535,"journal":{"name":"Al-Kimia","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41313472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identifikasi Komponen Senyawa Organik dan Uji Aktivitas Antiinflamasi dari Fraksi Etil Asetat Daun Cabai Rawit (Capsicum frutescens L.) 乙基资产组分Cabai Rawit(Capsicum frutescens L.)有机智能成分的鉴定及抗炎活性测试
Al-Kimia Pub Date : 2022-06-04 DOI: 10.24252/al-kimia.v10i1.20456
Uci Pradina, Ajuk Sapar, Warsidah Warsidah, Endah Sayekti, A. B. Aritonang
{"title":"Identifikasi Komponen Senyawa Organik dan Uji Aktivitas Antiinflamasi dari Fraksi Etil Asetat Daun Cabai Rawit (Capsicum frutescens L.)","authors":"Uci Pradina, Ajuk Sapar, Warsidah Warsidah, Endah Sayekti, A. B. Aritonang","doi":"10.24252/al-kimia.v10i1.20456","DOIUrl":"https://doi.org/10.24252/al-kimia.v10i1.20456","url":null,"abstract":"Cayenne pepper was known to treat swelling of tissue cells and is thought to have anti-inflammatory activity. The aim study is to examine the anti-inflammatory activity of the ethyl acetate fraction of cayenne pepper and identify the components of organic compounds in the ethyl acetate fraction. The stages of this research include preparation, extraction, partitioning, phytochemical tests, anti-inflammatory activity tests using Human Red Blood Cells (HRBC), and identifying the components of organic compounds they contain. Among the three fractions obtained at the extraction stage, the ethyl acetate fraction had the best activity as an anti-inflammatory at a concentration of 1000 mg/L of 57.77%. The fractionation of the ethyl acetate fraction was carried out using Vaccum Liquid Chromatography (VLC), and four combined fractions were obtained. One of the combined fractions, code EA-4-5, with a weight of 0.188 grams, was analyzed using GC-MS and FTIR. The two main compounds obtained from the GC-MS analysis were dodecanoic acid (25.79%) and (-)-Loliolide (12.86%). The results of the FTIR interpretation of the EA-4-5 fraction showed absorption at a wave number of 3234.62 cm-1 (stretching vibration of the -OH functional group), 3088.03 cm-1 and 3028.24 cm-1 (stretching vibration of the functional group =CH), 2931.8 cm-1 and 2858.51 cm-1 (functional group strain vibration –CH), 1712.79 cm-1 (functional group strain vibration –C=O), and 1606.7 cm-1 (strain vibration functional group –C=C).","PeriodicalId":7535,"journal":{"name":"Al-Kimia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48362405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Enkapsulasi Urea Menggunakan Biokomposit Zeolit Alam-Alginat-Pati Sagu sebagai Model Pupuk Lepas Lambat (Slow Release Fertilizer) 使用缓释肥料封装尿素
Al-Kimia Pub Date : 2022-06-03 DOI: 10.24252/al-kimia.v10i1.23739
Ida Ayu Suci, Ismail Astar
{"title":"Enkapsulasi Urea Menggunakan Biokomposit Zeolit Alam-Alginat-Pati Sagu sebagai Model Pupuk Lepas Lambat (Slow Release Fertilizer)","authors":"Ida Ayu Suci, Ismail Astar","doi":"10.24252/al-kimia.v10i1.23739","DOIUrl":"https://doi.org/10.24252/al-kimia.v10i1.23739","url":null,"abstract":"The urea fertilizer was encapsulation with the compositions of natural zeolite-alginate-sago starch biocomposite for developing of slowed-release properties. The characteristics of biocomposites such as FTIR, SEM, and nitrogen release tests were evaluated. Fourier transform infrared spectroscopy showed the presence of natural zeolite-alginate-sago starch biocomposite. The SEM images showed the surface morphology of urea has been coated by natural zeolite-alginate-sago starch biocomposite. Nitrogen release tests on natural zeolite-alginate-sago starch biocomposites with concentrations of 20% was the composition with the best results in holding urea encapsulated. This study indicates that natural zeolite-alginate-sago starch biocomposites are a alternative candidate for the development of the efficient slowed-release formulation of urea.","PeriodicalId":7535,"journal":{"name":"Al-Kimia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44052552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Potensi Senyawa Betalain pada Ekstrak Biji Binahong Berbatang Merah (Anredera cordifolia) sebagai Fotosensitizer Dye Sensitized Solar Cell (DSSC) 碧吉红提取物作为染料敏化太阳能电池(DSSC)的固溶性研究
Al-Kimia Pub Date : 2021-12-31 DOI: 10.24252/al-kimia.v9i2.20610
N. Cahyani, I. Sanjaya
{"title":"Potensi Senyawa Betalain pada Ekstrak Biji Binahong Berbatang Merah (Anredera cordifolia) sebagai Fotosensitizer Dye Sensitized Solar Cell (DSSC)","authors":"N. Cahyani, I. Sanjaya","doi":"10.24252/al-kimia.v9i2.20610","DOIUrl":"https://doi.org/10.24252/al-kimia.v9i2.20610","url":null,"abstract":"","PeriodicalId":7535,"journal":{"name":"Al-Kimia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47978400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Determination of Zirconium(IV) Based on Flow Injection Analysis with The Xylenol Orange As A Complexing 二甲酚橙络合流动注射法测定锆(IV)
Al-Kimia Pub Date : 2021-12-31 DOI: 10.24252/al-kimia.v9i2.23638
W. Rustiah
{"title":"Determination of Zirconium(IV) Based on Flow Injection Analysis with The Xylenol Orange As A Complexing","authors":"W. Rustiah","doi":"10.24252/al-kimia.v9i2.23638","DOIUrl":"https://doi.org/10.24252/al-kimia.v9i2.23638","url":null,"abstract":"The development of spectrophotometry method based on Flow Injection Analysis (FIA) to determine Zr(IV) in tin ore accompanying mineral has been done using xylenol orange as complexing agent. The complex between Zr(IV) and xylenol orange 0,05% was formed at pH 2 (1:1). The complex formed having maximum absorbance at wavelength 554 nm. The mineral decomposition has been done by fusion method using Na2CO3 and Na2O2 as fluxs for open system and the close system decomposition using HNO3, HClO4 and HF as solvents. FIA-spectrophotometry that has been developed was able to show good analytical performance, with precision of %KV not more than 5% at concentration level mg/L, was 3,98%, with sensitivity was 2,79 ppm and the detection limits of calculated (S/N = 3) was 0,21 ppm. This method has been aplicated for analyzing Zr(IV) in zircon mineral sample with analysis result 20,26% of ZrO2 and recovery percentage was 100,7%. ","PeriodicalId":7535,"journal":{"name":"Al-Kimia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45879594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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