WormBook : the online review of C. elegans biology最新文献_第5页

Transcriptional regulation of gene expression in C. elegans. 秀丽隐杆线虫基因表达的转录调控。

WormBook : the online review of C. elegans biology Pub Date : 2013-06-04 DOI: 10.1895/wormbook.1.45.2

Valerie Reinke, Michael Krause, Peter Okkema

引用次数: 39

Pristionchus pacificus protocols. 和平监狱协议。

WormBook : the online review of C. elegans biology Pub Date : 2013-03-14 DOI: 10.1895/wormbook.1.114.2

Andre Pires-daSilva

引用次数: 18

Keeping track of worm trackers. 跟踪蠕虫跟踪器。

WormBook : the online review of C. elegans biology Pub Date : 2013-02-22 DOI: 10.1895/wormbook.1.156.1

Steven J Husson, Wagner Steuer Costa, Cornelia Schmitt, Alexander Gottschalk

{"title":"Keeping track of worm trackers.","authors":"Steven J Husson, Wagner Steuer Costa, Cornelia Schmitt, Alexander Gottschalk","doi":"10.1895/wormbook.1.156.1","DOIUrl":"https://doi.org/10.1895/wormbook.1.156.1","url":null,"abstract":"C. elegans is used extensively as a model system in the neurosciences due to its well defined nervous system. However, the seeming simplicity of this nervous system in anatomical structure and neuronal connectivity, at least compared to higher animals, underlies a rich diversity of behaviors. The usefulness of the worm in genome-wide mutagenesis or RNAi screens, where thousands of strains are assessed for phenotype, emphasizes the need for computational methods for automated parameterization of generated behaviors. In addition, behaviors can be modulated upon external cues like temperature, O(subscript)2(/subscript) and CO(subscript)2(/subscript) concentrations, mechanosensory and chemosensory inputs. Different machine vision tools have been developed to aid researchers in their efforts to inventory and characterize defined behavioral \"outputs\". Here we aim at providing an overview of different worm-tracking packages or video analysis tools designed to quantify different aspects of locomotion such as the occurrence of directional changes (turns, omega bends), curvature of the sinusoidal shape (amplitude, body bend angles) and velocity (speed, backward or forward movement).","PeriodicalId":75344,"journal":{"name":"WormBook : the online review of C. elegans biology","volume":" ","pages":"1-17"},"PeriodicalIF":0.0,"publicationDate":"2013-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4781246/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31261394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 113

Cell isolation and culture. 细胞分离和培养。

WormBook : the online review of C. elegans biology Pub Date : 2013-02-21 DOI: 10.1895/wormbook.1.157.1

Sihui Zhang, Jeffrey R Kuhn

引用次数: 36

Ascaroside signaling in C. elegans. 秀丽隐杆线虫的天蚕苷信号传导。

WormBook : the online review of C. elegans biology Pub Date : 2013-01-18 DOI: 10.1895/wormbook.1.155.1

Andreas H Ludewig, Frank C Schroeder

{"title":"Ascaroside signaling in C. elegans.","authors":"Andreas H Ludewig, Frank C Schroeder","doi":"10.1895/wormbook.1.155.1","DOIUrl":"https://doi.org/10.1895/wormbook.1.155.1","url":null,"abstract":"Over the past 10 years, the relevance of small-molecule signaling for many aspects of C. elegans development and behavior has become apparent. One prominent group of small-molecule signals are the ascarosides, which control dauer entry and exit as well as a variety of sex-specific and social behaviors, including male attraction, hermaphrodite repulsion, olfactory plasticity, and aggregation. This wide range of biological functions is facilitated by a great diversity of ascaroside chemical structures. These are based on the sugar ascarylose, which is linked to fatty acid-like side chains of varying lengths and often decorated further with building blocks derived from amino acids, folate, and other primary metabolites. Different ascarosides or combinations of ascarosides mediate different phenotypes, and even small differences in chemical structures are often associated with strongly altered activity profiles. Additional complexity arises from concentration-dependent effects and synergism between different ascarosides. The ascarosides are sensed by several types of chemosensory head neurons, including the ASK, ASI, and ADL neurons as well as the male-specific CEM neurons. Ascaroside perception is mediated by diverse families of G-protein coupled membrane receptors that act upstream of conserved signal transduction pathways, including insulin/IGF-1 signaling and transforming growth factor beta (TGF-β) signaling. Biosynthesis of the ascarosides appears to integrate input from several primary metabolic pathways, including peroxisomal β-oxidation of long-chain fatty acids and amino acid catabolism. Life stage, sex, as well as food availability and other environmental factors affect ascaroside biosynthesis, suggesting that ascaroside signaling communicates detailed information about life history and metabolic state.","PeriodicalId":75344,"journal":{"name":"WormBook : the online review of C. elegans biology","volume":" ","pages":"1-22"},"PeriodicalIF":0.0,"publicationDate":"2013-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3758900/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31192874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 177

Notch signaling: genetics and structure. 缺口信号:遗传和结构。

WormBook : the online review of C. elegans biology Pub Date : 2013-01-17 DOI: 10.1895/wormbook.1.10.2

Iva Greenwald, Rhett Kovall

{"title":"Notch signaling: genetics and structure.","authors":"Iva Greenwald, Rhett Kovall","doi":"10.1895/wormbook.1.10.2","DOIUrl":"https://doi.org/10.1895/wormbook.1.10.2","url":null,"abstract":"Receptors of the Notch family mediate cell-cell interactions during animal development, and aberrations in Notch signaling have been implicated in human disease. Studies in Caenorhabdits elegans have made essential contributions towards understanding the biological roles and molecular mechanism of this fundamental signaling system. A major development in the field since the original version of this chapter (LIN-12/Notch signaling in C. elegans) has been an explosion in information about the structural biology of Notch signaling; crystallographic determinations of structures, including structures of C. elegans components, have contributed much to the current understanding of molecular mechanism. Thus, here, we not only cover the genetics of Notch in C. elegans, focusing on conserved core components and modulators, we have also included structural information about these components, describing the key events occurring during ligand binding and transcriptional control of target genes. In addition to text, we include Tables listing core components and key modulators of the signaling pathway along with their orthologs in Drosophila and mammals, a Table listing validated target genes in various processes in C. elegans, and animated features to illustrate structural attributes.","PeriodicalId":75344,"journal":{"name":"WormBook : the online review of C. elegans biology","volume":" ","pages":"1-28"},"PeriodicalIF":0.0,"publicationDate":"2013-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5402211/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31192873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 100

Methods for measuring pharyngeal behaviors. 测量咽部行为的方法。

WormBook : the online review of C. elegans biology Pub Date : 2012-12-18 DOI: 10.1895/wormbook.1.154.1

David Raizen, Bo-Mi Song, Nick Trojanowski, Young-Jai You

引用次数: 78

Single molecule fluorescent in situ hybridization (smFISH) of C. elegans worms and embryos. 秀丽隐杆线虫蠕虫和胚胎的单分子荧光原位杂交技术。

WormBook : the online review of C. elegans biology Pub Date : 2012-12-13 DOI: 10.1895/wormbook.1.153.1

Ni Ji, Alexander van Oudenaarden

引用次数: 98

Morphogenesis of the vulva and the vulval-uterine connection. 外阴的形态发生和外阴与子宫的连接。

WormBook : the online review of C. elegans biology Pub Date : 2012-11-30 DOI: 10.1895/wormbook.1.152.1

Bhagwati P Gupta, Wendy Hanna-Rose, Paul W Sternberg

{"title":"Morphogenesis of the vulva and the vulval-uterine connection.","authors":"Bhagwati P Gupta, Wendy Hanna-Rose, Paul W Sternberg","doi":"10.1895/wormbook.1.152.1","DOIUrl":"https://doi.org/10.1895/wormbook.1.152.1","url":null,"abstract":"The C. elegans hermaphrodite vulva is an established model system to study mechanisms of cell fate specification and tissue morphogenesis. The adult vulva is a tubular shaped organ composed of seven concentric toroids that arise from selective fusion between differentiated vulval progeny. The dorsal end of the vulval tubule is connected to the uterus via a multinucleate syncytium utse (uterine-seam) cell. The vulval tubule and utse are formed as a result of changes in morphogenetic processes such as cell polarity, adhesion, and invagination. A number of genes controlling these processes are conserved all the way up to human and function in similar developmental contexts. This makes it possible to extend the findings to other metazoan systems. Gene expression studies in the vulval and uterine cells have revealed the presence of regulatory networks specifying distinct cell fates. Thus, these two cell types serve as a good system to understand how gene networks confer unique cell identities both experimentally and computationally. This chapter focuses on morphogenetic processes during the formation of the vulva and its connection to uterus.","PeriodicalId":75344,"journal":{"name":"WormBook : the online review of C. elegans biology","volume":" ","pages":"1-20"},"PeriodicalIF":0.0,"publicationDate":"2012-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5402222/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31095823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 46

Eph receptor signaling in C. elegans. 秀丽隐杆线虫的Eph受体信号传导。

WormBook : the online review of C. elegans biology Pub Date : 2012-11-29 DOI: 10.1895/wormbook.1.151.1

Michael A Miller, Ian D Chin-Sang

引用次数: 6