World journal of experimental medicine最新文献

{"title":"Cytoplasmic and nuclear programmed death ligand 1 expression in peritumoral stromal cells in breast cancer: Prognostic and predictive value.","authors":"Evgeniya Yu Zubareva, Marina A Senchukova, Natalia V Saidler","doi":"10.5493/wjem.v15.i2.102761","DOIUrl":"10.5493/wjem.v15.i2.102761","url":null,"abstract":"<p><strong>Background: </strong>Breast cancer (BC) continues to occupy a leading position in terms of morbidity and mortality from malignant neoplasms among the female population. One of the promising markers associated with BC progression is programmed death ligand 1 (PD-L1). Previously, we investigated PD-L1 expression in BC <i>via</i> a new antibody against programmed cell death protein 1 ligand 1 (PDCD1 LG1) and reported that high PDCD1 LG1 expression in tumor cells is an independent factor for a high risk of regional metastasis in patients with BC. However, the prognostic significance of PDCD1 LG1 expression in BC stromal cells has not been adequately studied.</p><p><strong>Aim: </strong>To study the features of PDCD1 LG1 expression in BC stromal cells and its relationship with BC clinicopathological characteristics.</p><p><strong>Methods: </strong>In a prospective single-center observational study, tumor samples from 148 patients with newly diagnosed BC were examined. The tumor sections were immunohistochemically stained with antibodies against PDCD1 LG1. In the tumor samples, the PDCD1 LG1-positive lymphocyte (PDCD1 LG1+ LF) score, presence of nuclear PDCD1 LG1 expression in the LFs, PDCD1 LG1 expression in polymorphic cell infiltrates (PDCD1 LG1+ polymorphic cell infiltrates [PCIs]), and cells of the fibroblastic stroma and endothelial cells of the tumor microvessels were assessed. Statistical analyses were performed using Statistica 10.0 software.</p><p><strong>Results: </strong>A PDCD1 LG1+ LF score ≥ 3 was detected more often at stages N0 and N3 than at N1 and N2 (<i>P</i> = 0.03). Moderate and pronounced PDCD1 LG1+ PCIs and the presence of PDCD1 LG1+ fibroblastic stroma were associated with negative estrogen receptor status (<i>P</i> = 0.0008 and <i>P</i> = 0.03, respectively), human epidermal growth factor receptor 2-positive (HER2+) BC (<i>P</i> < 0.00001 and <i>P</i> = 0.0005), and luminal B HER2+, non-luminal HER2+ and triple-negative BC (<i>P</i> < 0.00001 and <i>P</i> = 0.004). The risk of metastasis to regional lymph nodes (RLNs) depend on lymphovascular invasion (LVI) and the PDCD1 LG1+ LF score. In the absence of LVI and a PDCD1 LG1+ LF score < 3 or ≥ 3, metastases in RLNs were absent in 66.6% and 93.9% of patients with BC, respectively. In the presence of LVI and a PDCD1 LG1+ LF score < 3 or ≥ 3, metastases in RLNs were detected in 82.6% and 92.7% of patients with BC, respectively.</p><p><strong>Conclusion: </strong>The results indicated that the combined assessment of the PDCD1 LG1+ LF score and LVI can improve the accuracy of predicting the risk of metastasis to RLNs in patients with BC.</p>","PeriodicalId":75340,"journal":{"name":"World journal of experimental medicine","volume":"15 2","pages":"102761"},"PeriodicalIF":0.0,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12019619/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144369679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating fever of unknown origin definitions in a tertiary care setting: Implications for diagnostic criteria revision.","authors":"Pathik Dhangar, Prasan Kumar Panda, Ravi Kant, Rohit Gupta, Ruchi Dua, Ashutosh Tiwari, Sandeep Saini, Kavita Khoiwal, Yogesh Bahurupi","doi":"10.5493/wjem.v15.i2.101388","DOIUrl":"10.5493/wjem.v15.i2.101388","url":null,"abstract":"<p><strong>Background: </strong>Fever of unknown origin (FUO) remains a diagnostic challenge and was originally defined in 1961. Its classic criteria include fever ≥ 38.3 °C (≥ 101 °F) on multiple occasions, fever lasting three weeks or longer, and a diagnosis after one week of inpatient evaluation. However, these criteria may not fully encompass the varied clinical presentations seen in resource-limited settings such as India. The adaptation of FUO definitions to local healthcare contexts is crucial for enhancing diagnostic accuracy and optimizing patient outcomes.</p><p><strong>Aim: </strong>To investigate the applicability of revised FUO criteria in a tertiary care setting in India.</p><p><strong>Methods: </strong>This longitudinal-exploratory study at All India Institute of Medical Sciences Rishikesh (January 2018-December 2022) analyzed 228 adult patients with fever ≥ 99.1 °F lasting over three days. Patients diagnosed within three days of admission were excluded. Data were collected retrospectively and prospectively using predefined FUO definitions based on durations of nondiagnosis (3-21 days, > 21 days), temperature ranges (99.1 °F-100.9 °F, ≥ 101 °F), and hospitalization durations (3-7 days, > 7 days). Descriptive statistics and comparative tests (Fisher's exact test, <i>χ</i> ² test) evaluated outcomes across definitions.</p><p><strong>Results: </strong>Among the proposed FUO definitions, Definition B (fever lasting 3-21 days, temperatures between 99.1 °F-100.9 °F, hospitalization > 7 days) predominated (40.8%), while only 2.2% met the classical criteria. Notably, 36.5% of Definition B patients remained undiagnosed after 7-10 days, despite 94% undergoing diagnostic workups within 21 days. Infection emerged as the leading etiology across definitions, without significant variation in outcomes or mortality during hospitalization (<i>χ</i> ² = 27.937, <i>P</i> = 0.142).</p><p><strong>Conclusion: </strong>Adapting FUO criteria to local contexts improves diagnostic accuracy and treatment. Definition B (40.8% prevalence) showed practical utility, with higher mortality in patients discharged on empirical 'Anti-tuberculosis therapy'.</p>","PeriodicalId":75340,"journal":{"name":"World journal of experimental medicine","volume":"15 2","pages":"101388"},"PeriodicalIF":0.0,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12019626/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144369684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-hypertensive effect of enriched white melon seed protein concentrate biscuit on sodium fluoride exposed rats.","authors":"Olamide Wilson Fasakin, Ayoola Awosika, Sanmi Tunde Ogunsanya, Iyanuoluwa Olushola Benson, Akinyode Isaac Olopoda","doi":"10.5493/wjem.v15.i2.105798","DOIUrl":"10.5493/wjem.v15.i2.105798","url":null,"abstract":"<p><strong>Background: </strong>Sodium fluoride (NaF) is a daily necessity consumed as the major ingredient of fluorinated drinking water, milk, salts, mouthwashes, toothpaste, and dentistry medications. However, the use of NaF products has also been associated with increased fluoride anion distribution in the body, leading to hypertension.</p><p><strong>Aim: </strong>This study evaluated the antihypertensive effect of sweet orange peels-enriched white melon seed protein concentrate (WSP) biscuit meal in eight-week-old albino rats exposed to NaF for 14 days.</p><p><strong>Methods: </strong>Forty-two (42) male Wistar albino rats were assigned at random into 7 groups of 6 rats per group (control group and six experimental groups). The experimental groups received various treatments that lasted for two weeks. Twenty-four hours after the last administration, hemodynamic parameters were evaluated, rats were sacrificed, blood samples were collected, and the heart was harvested. Blood serum was assessed for cardiac troponin I (cTnI), creatine kinase-MB (CK-MB), and lactate dehydrogenase (LDH). At the same time, the heart homogenate was assayed for angiotensin-1 converting enzyme (ACE) activity, proinflammatory cytokines, nitric oxide concentrations, and antioxidant status. Cardiac tissues were stained with Hematoxylin and Eosin, Masson's Trichrome, and cTnI. Also, the safety of the WSP biscuit diet was evaluated.</p><p><strong>Results: </strong>Results obtained showed that NaF administration elevated the collagen content of cardiac tissues, activities of ACE, and concentrations of cTnI, CK-MB, LDH, tumor necrosis factor-alpha, and interleukin 1 beta, while there was a reduction in the concentration of nitric oxide and antioxidants; however, their alterations were significantly prevented in WSP-biscuit-fed rats. The WSP biscuit meal is safe for consumption and possesses dose-dependent antihypertensive ability at 10% and 20% inclusion.</p><p><strong>Conclusion: </strong>The WSP biscuit diet may be recommended in diet formulation for the management of individuals or communities that are predisposed to NaF contaminations.</p>","PeriodicalId":75340,"journal":{"name":"World journal of experimental medicine","volume":"15 2","pages":"105798"},"PeriodicalIF":0.0,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12019614/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144369677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miRNA dysregulation in Duchenne muscular dystrophy comorbidities.","authors":"Subhashree Sivakumar, Archana Rajavel, Venkataraman Viswanathan, Evangeline Ann Daniel, Prakash Gangadaran, Raja Natesan Sella","doi":"10.5493/wjem.v15.i2.100548","DOIUrl":"10.5493/wjem.v15.i2.100548","url":null,"abstract":"<p><strong>Background: </strong>Duchenne muscular dystrophy (DMD) is a neuromuscular disorder caused by mutations in the dystrophin gene. DMD is reported to coexist with other comorbidities, although the occurrence of the triad, autism spectrum disorder (ASD), and epilepsy is very rare. Indeed, only one case of the triad has currently been reported. Here, we present a detailed case report of a ten-year-old boy with DMD, ASD, and epilepsy. We also investigated the dysregulation of miRNAs in this unusual triad (represented as DMD++) compared with a healthy individual and a DMD patient (represented as DMD+) without autism.</p><p><strong>Aim: </strong>To understand the differential expression of miRNAs in rare comorbid DMD cases.</p><p><strong>Methods: </strong>The Sequin Form Board test, Gesell's drawing test, multiplex ligation probe amplification, and Vineland Social Maturity Scale were applied to confirm the DMD and ASD. Total RNA was isolated from samples using TRIzol. cDNA was synthesized using the Mir-X™ miRNA First-Strand Synthesis kit. qRT-PCR was performed using SYBR Advantage qPCR Premix. The results were statistically analyzed using one-way analysis of variance with Tukey's <i>t</i>-test.</p><p><strong>Results: </strong>miR-146a-5p and miR-132-5p showed significant downregulation in both patient samples. miR-199a-5p and miR-146a-3p showed no change in expression between the diseased and controls. miR-132-3p showed downregulation only in the DMD+ sample (0.21 ± 0.04). The decrease in miR-132-3p can result in failed silencing of the phosphatase and tensin homolog-mediated apoptotic pathway, leading to severe skeletal muscle atrophy. Here, the downregulation of miR-132-3p in DMD+ is consistent with severe muscle loss and higher disease progression than that in DMD++. DMD++ has slower disease progression, and the expression of miRNA involved in inflammatory and apoptotic responses is more similar to that of the control.</p><p><strong>Conclusion: </strong>Our study shows marked difference in miRNA expression in this rare case of DMD with autism and epilepsy. These miRNAs also serve as regulators of several muscle regeneration, apoptosis, and inflammatory pathways. This study shows the significance of studying miRNAs in such rare cases in a larger cohort to progress in several intervention treatments utilizing miRNAs.</p>","PeriodicalId":75340,"journal":{"name":"World journal of experimental medicine","volume":"15 2","pages":"100548"},"PeriodicalIF":0.0,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12019623/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144369692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomarkers for tracking metabolic changes pre-post nutritional epigenetics diet/intervention to prevent autism and attention deficit/hyperactivity disorders in children.","authors":"Renee J Dufault","doi":"10.5493/wjem.v15.i2.101555","DOIUrl":"10.5493/wjem.v15.i2.101555","url":null,"abstract":"<p><p>The prevalence of autism and attention deficit/hyperactivity disorders is increasing worldwide. Recent studies suggest the excessive intake of ultra-processed food plays a role in the inheritance of these disorders <i>via</i> heavy metal exposures and nutritional deficits that impact the expression of genes. In the case of the metallothionein (MT) gene, biomarker studies show dietary zinc (Zn) deficits impact MT protein levels in children with autism and are associated with the bioaccumulation of lead and/or mercury in children exhibiting autism/attention deficit/hyperactivity disorders symptomology. The impact of dietary changes on lead and mercury exposures and <i>MT</i> gene behavior could be determined using a randomized test and control group design. Pregnant women serving in the test-group would participate in a nutritional epigenetics education intervention/course designed to reduce ultra-processed food intake and heavy metal levels in blood while increasing whole food intake and MT and Zn levels. Changes in maternal diet would be measured using data derived from an online diet survey administered to the test and control groups pre-post intervention. Changes in maternal lead, mercury, Zn, and MT levels would be measured <i>via</i> blood sample analyses prior to the intervention and after childbirth <i>via</i> cord blood analyses to determine infant risk factors.</p>","PeriodicalId":75340,"journal":{"name":"World journal of experimental medicine","volume":"15 2","pages":"101555"},"PeriodicalIF":0.0,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12019618/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144369678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Matrix metalloproteinases and their tissue inhibitors as indicators of aortic aneurysm and dissection development in extracellular matrix remodeling.","authors":"Marc Irqsusi, Fiona R Rodepeter, Madeline Günther, Andreas Kirschbaum, Sebastian Vogt","doi":"10.5493/wjem.v15.i2.100166","DOIUrl":"10.5493/wjem.v15.i2.100166","url":null,"abstract":"<p><p>Aneurysms and dissections represent some of the most serious cardiovascular diseases. The prevailing theory posits that mechanical overloading of the vessel wall is the underlying cause. Inspired by Barkhordarian <i>et al</i>, the authors present matrix metalloproteinases (MMPs) and their inhibitors in immunohistological analyses as contributing factors in the pathophysiology of aortic aneurysms (AA). Data analysis of MMP-1, MMP-9, tissue inhibitors of metalloproteinases (TIMPs), including TIMP-1 and TIMP-2 expression reveals a varied distribution between the adventitia and media and a non-uniform expression of the investigated markers. These elements, as key components of the extracellular matrix (ECM), indicate that the formation of AA is not solely driven by endoluminal pressure loading of the aortic wall. Instead, degenerative processes within ECM elements contribute significantly. Importantly, AA do not necessarily imply dissection. Tissue destruction, allowing blood flow entry, arises from reduced oxygen supply to the media, primarily due to incomplete capillarization or neocapillarization.</p>","PeriodicalId":75340,"journal":{"name":"World journal of experimental medicine","volume":"15 2","pages":"100166"},"PeriodicalIF":0.0,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12019616/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144369690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Platelet rich plasma, adipose tissue micrografts, and regenerative mimetic factors for abdominal wall defect reconstruction: Experimental study protocol.","authors":"Konstantinos Zapsalis, Orestis Ioannidis, Christos Xylas, Konstantinos Siozos, Georgios Gemousakakis, Elissavet Anestiadou, Savvas Symeonidis, Stefanos Bitsianis, Efstathios Kotidis, Angeliki Cheva, Chryssa Bekiari, Antonia Loukousia, Konstantinos Angelopoulos, Manousos-Georgios Pramateftakis, Ioannis Mantzoros, Freiderikos Tserkezidis, Barbara Driagka, Stamatios Angelopoulos","doi":"10.5493/wjem.v15.i2.99065","DOIUrl":"10.5493/wjem.v15.i2.99065","url":null,"abstract":"<p><strong>Background: </strong>Incisional hernias are a common complication of previous surgeries and remain a persistent issue in clinical practice, posing a significant burden on healthcare systems despite advances in education and technology. Surgical techniques, primarily involving the use of mesh to cover the abdominal wall gap, are widely used as a standard intervention strategy.</p><p><strong>Aim: </strong>To examine the regeneration of the aponeurosis defect in the anterior abdominal wall in rats using regenerative mimetic factors of the extracellular matrix [ReGeneraTing Agent (RGTA)], adipose tissue micrografts (ATM), and platelet rich plasma (PRP) as regenerative agents.</p><p><strong>Methods: </strong>Regenerative agents such as RGTA, ATM, and PRP are gaining popularity. ATM involves autologous adipose tissue cells with mesenchymal stem cell markers and a high percentage of stromal vascular fraction cells. RGTAs are heparan sulfate (HS) mimetics that replace degraded HSs in damaged tissue, enhancing the quality and speed of repair. PRP is a concentrated plasma preparation containing seven fundamental proteins responsible for tissue production. An acellular dermal matrix is a biological implant free of cellular or antigenic components, making it an excellent material for reconstructive surgery. Polyglactin is a synthetic, absorbable mesh that loses 50% of its strength after fourteen days, providing initial support for new tissue regeneration before being completely absorbed.</p><p><strong>Results: </strong>Rats will undergo a laparotomy with a precise 2 cm by 2 cm excision of the anterior abdominal wall fascia below the umbilicus. They will be divided into sixteen groups, each receiving different combinations of regenerative factor injections into the denervated area in both non-contaminated and contaminated environments. A collagen-elastin matrix will be used to join the aponeurosis edges, with an absorbable polyglactin mesh anchored over it. Samples will be taken for macroscopic, histological, and immunohistochemical evaluation of tissue regeneration.</p><p><strong>Conclusion: </strong>Our study aims to demonstrate how these factors promote cell proliferation and healing of the denervated anterior abdominal wall, potentially reducing the frequency and complications of incisional hernias. This approach could offer a more economical and efficient treatment option compared to current costly methods.</p>","PeriodicalId":75340,"journal":{"name":"World journal of experimental medicine","volume":"15 2","pages":"99065"},"PeriodicalIF":0.0,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12019615/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144369693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hypoxia-inducible factor-1α at the invasive tumor front in oral squamous cell carcinoma.","authors":"Felipe Martins Silveira, Lauren Frenzel Schuch, Vanesa Pereira-Prado, Nelly Molina-Frechero, Sandra Lopez-Verdin, Marcelo Gómez Palacio-Gastélum, Miguel Arocena, Sven Niklander, Estefania Sicco, Ronell Bologna-Molina","doi":"10.5493/wjem.v15.i2.102175","DOIUrl":"10.5493/wjem.v15.i2.102175","url":null,"abstract":"<p><strong>Background: </strong>Hypoxia in oral cancer promotes tumoral invasion by inducing epithelial-mesenchymal transition, leading to aggressive tumor progression.</p><p><strong>Aim: </strong>To characterize the expression of hypoxia-inducible factor 1-alpha (HIF-1α) at the invasive tumor front (ITF) in comparison to tumor islands (TI) in oral squamous cell carcinoma (OSCC) and to explore its relationship with E-cadherin and Vimentin expression.</p><p><strong>Methods: </strong>Thirty-eight cases of OSCC and five cases of normal oral mucosa (NOM) were included in this study. The ITF was identified based on the region and immune expression of AE1/AE3. Immunohistochemistry was performed to assess the expression of HIF-1α, Vimentin, and E-cadherin. The immunostaining was analyzed using an immunoreactive score, and the results were illustrated using immunofluorescence.</p><p><strong>Results: </strong>HIF-1α expression was significantly higher in the TI region compared to the ITF region (<i>P</i> = 0.0134). Additionally, a significant difference was observed between TI and NOM (<i>P</i> = 0.0115). In the ITF regions, HIF-1α expression showed a significant correlation with Vimentin expression, with higher levels of HIF-1α associated with increased Vimentin expression (<i>P</i> = 0.017).</p><p><strong>Conclusion: </strong>Based on the results of this study, HIF-1α appears to play a distinct role in OSCC tumor progression, underscoring the importance of exploring hypoxia-driven changes in cellular phenotype at the ITF of OSCC. Further research is needed to better understand their impact on OSCC prognosis.</p>","PeriodicalId":75340,"journal":{"name":"World journal of experimental medicine","volume":"15 2","pages":"102175"},"PeriodicalIF":0.0,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12019610/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144369687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the nutritional challenges in epilepsy: Risks, deficiencies, and management strategies: A systematic review.","authors":"Mohammed Al-Beltagi, Nermin Kamal Saeed, Adel Salah Bediwy, Reem Elbeltagi","doi":"10.5493/wjem.v15.i2.104328","DOIUrl":"10.5493/wjem.v15.i2.104328","url":null,"abstract":"<p><strong>Background: </strong>Malnutrition and epilepsy share a complex bidirectional relationship, with malnutrition serving as a potential risk factor for epilepsy development, while epilepsy, in turn, often exerts profound effects on nutritional status. Nutritional interventions have emerged as a critical adjunctive approach in epilepsy management.</p><p><strong>Aim: </strong>To explore the multifaceted associations between malnutrition and epilepsy, structured into three primary sections: (1) Elucidating the impact of malnutrition as a risk factor for epilepsy onset; (2) Examining the reciprocal influence of epilepsy on nutritional status, and (3) Evaluating diverse nutritional interventions in the management of epilepsy.</p><p><strong>Methods: </strong>A systematic search was conducted across PubMed, Scopus, and Web of Science databases utilizing defined keywords related to malnutrition, epilepsy, and nutritional interventions. Inclusion criteria encompassed various study types, including clinical trials, animal models, cohort studies, case reports, meta-analyses, systematic reviews, guidelines, editorials, and review articles. Four hundred sixteen pertinent references were identified, with 198 review articles, 153 research studies, 21 case reports, 24 meta-analyses, 14 systematic reviews, 4 guidelines, and 2 editorials meeting the predefined criteria.</p><p><strong>Results: </strong>The review revealed the intricate interplay between malnutrition and epilepsy, highlighting malnutrition as a potential risk factor in epilepsy development and elucidating how epilepsy often leads to nutritional deficiencies. Findings underscored the importance of nutritional interventions in managing epilepsy, showing their impact on seizure frequency, neuronal function, and overall brain health.</p><p><strong>Conclusion: </strong>This systematic review emphasizes the bidirectional relationship between malnutrition and epilepsy while emphasizing the critical role of nutritional management in epilepsy treatment. The multifaceted insights underscore the need for a holistic approach to addressing nutritional aspects alongside conventional epilepsy management strategies.</p>","PeriodicalId":75340,"journal":{"name":"World journal of experimental medicine","volume":"15 2","pages":"104328"},"PeriodicalIF":0.0,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12019608/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144369694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diarylpentanoid, a curcumin analog, inhibits malignant meningioma growth in both <i>in vitro</i> and <i>in vivo</i> models.","authors":"Anna Terasawa, Kazuhiro Shimazu, Hiroshi Nanjo, Masatomo Miura, Hiroyuki Shibata","doi":"10.5493/wjem.v15.i2.102897","DOIUrl":"10.5493/wjem.v15.i2.102897","url":null,"abstract":"<p><strong>Background: </strong>Malignant meningioma metastasizes systemically, primarily due to its role in epithelial-mesenchymal transition. Although the prognosis is extremely poor, drug development efforts have been limited, because this tumor is categorized as a rare form.</p><p><strong>Aim: </strong>To examine growth suppressive effect of GO-Y030, a diarylpentanoid curcumin analog, (1E,4E)-1,5-bis [3,5-bis (methoxymethoxy) phenyl] penta-1,4-dien-3-one against the malignant meningioma.</p><p><strong>Methods: </strong>The growth suppression of malignant meningioma cells by GO-Y022 and GO-Y030 were examined, using IOMM-Lee and HKBMM cell lines. Male nude mice aged eight weeks, specifically BALB/cSlc-nu/nu mice received a subcutaneous inoculation of IOMM-Lee (10⁷ cells/site) on their back and 30 μg/kg of recombinant hepatocellular growth factor (HGF) was injected into the tumor every three days. After confirmed the growth tumor mass, 500 μL of GO-Y030 diluted with PBS were administrated intraperitoneally daily at doses of 1 mg/kg and 2 mg/kg, respectively.</p><p><strong>Results: </strong>GO-Y030 exhibits a growth inhibitory effect on malignant meningioma cell lines, IOMM-Lee and HKBMM ranging from 0.8-2.0 μM <i>in vitro</i>. Notably, GO-Y030's inhibitory effect is about 10 to 16^th times more potent than that of curcumin, which has previously demonstrated potential in combating malignant meningioma. In mouse models, the intraperitoneal administration of GO-Y030 effectively suppresses the growth of malignant meningioma tumors that have been inoculated in the back (<i>P</i> = 0.002). High-performance liquid chromatography analysis has confirmed the distribution of GO-Y030 in the bloodstream and brain tissue. Moreover, GO-Y030 demonstrates the ability to significantly suppress HGF (<i>P</i> < 0.01), nuclear factor kappa B (<i>P</i> < 0.001), and N-cadherin (<i>P</i> < 0.001), all of which contribute to the epithelial-mesenchymal transition.</p><p><strong>Conclusion: </strong>GO-Y030 holds promise as a potent compound for the systemic inhibition of malignant meningioma. GO-Y030 has higher tumor growth inhibitory effect against meningiomas than curcumin, which is known to have antitumor activity through multi-molecular target control resulting in apoptosis induction. GO-Y030 controls at least three molecules of HGF, nuclear factor kappa B, and N-cadherin.</p>","PeriodicalId":75340,"journal":{"name":"World journal of experimental medicine","volume":"15 2","pages":"102897"},"PeriodicalIF":0.0,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12019622/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144369680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}