Urine (Amsterdam, Netherlands)最新文献

In addition to early biomarkers, what else can be done with urine? 除了早期生物标志物，尿液还能做什么？

Urine (Amsterdam, Netherlands) Pub Date : 2024-01-01 DOI: 10.1016/j.urine.2023.12.001

Yuzhen Chen, Youhe Gao

引用次数: 0

CreCENT: Creatinine and Chloride based Electrochemical Non-faradaic renal health mapping Technology CreCENT：基于肌酐和氯化物的电化学非法拉第肾脏健康绘图技术

Urine (Amsterdam, Netherlands) Pub Date : 2023-12-04 DOI: 10.1016/j.urine.2023.11.001

Antra Ganguly, Varun Gunda, Shalini Prasad

{"title":"CreCENT: Creatinine and Chloride based Electrochemical Non-faradaic renal health mapping Technology","authors":"Antra Ganguly, Varun Gunda, Shalini Prasad","doi":"10.1016/j.urine.2023.11.001","DOIUrl":"https://doi.org/10.1016/j.urine.2023.11.001","url":null,"abstract":"<div><p>In this work, we propose a novel biosensing scheme to enable the stratification of kidney diseases based on severity and progression for timely triage and efficient management. Here, using Creatinine and Chloride as target analytes for the biosensor, we have discussed an arbitrary three-class stratification mapping renal health for Chronic Kidney Disease (CKD) management. Our method is fully quantitative, fast (<5 min turn-around time), and can work with any combination of disease biomarkers to categorize diseases by subtypes and severity. At its core, the biosensor relies on electrochemical impedance spectroscopy to transduce subtle changes at the input Creatinine and Chloride levels in a drop of neat, unprocessed urine. It can operate over a wide dynamic range of 0.15-5 mg/mL for Creatinine and 15-105 mM for Chloride. Further, as proof of concept, the biosensing scheme utilizes a simple Support Vector Machine-based supervised machine learning model for 3-state output disease state classification (corresponding to low, medium, and high disease severity) with a 97.96% accuracy. This scheme is versatile and can be extended to more complex scenarios with more biomarker input stimuli for improved diagnostics and precision therapy for other chronic urological diseases.</p></div>","PeriodicalId":75287,"journal":{"name":"Urine (Amsterdam, Netherlands)","volume":"6 ","pages":"Pages 1-7"},"PeriodicalIF":0.0,"publicationDate":"2023-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S259028062300013X/pdfft?md5=40251a3a1c23df365d5168f1883b81ee&pid=1-s2.0-S259028062300013X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138557252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A facile strategy for comprehensive proteome analysis of urine using ultracentrifugation fractionation, solid-phase alkylation based sample preparation and liquid chromatography-mass spectrometry 使用超离心分离、固相烷基化样品制备和液相色谱-质谱法对尿液进行综合蛋白质组分析的简便策略

Urine (Amsterdam, Netherlands) Pub Date : 2023-01-01 DOI: 10.1016/j.urine.2023.05.002

Xinxin Liu , Mengchun Cheng , Zhigang Sui , Hongli Lin , Nan Wang , Xianan Guo , Huiming Yuan , Lihua Zhang , Yukui Zhang

{"title":"A facile strategy for comprehensive proteome analysis of urine using ultracentrifugation fractionation, solid-phase alkylation based sample preparation and liquid chromatography-mass spectrometry","authors":"Xinxin Liu , Mengchun Cheng , Zhigang Sui , Hongli Lin , Nan Wang , Xianan Guo , Huiming Yuan , Lihua Zhang , Yukui Zhang","doi":"10.1016/j.urine.2023.05.002","DOIUrl":"10.1016/j.urine.2023.05.002","url":null,"abstract":"<div><p>In this work, a facile strategy with combination of ultracentrifugation fraction, solid-phase alkylation sample preparation and liquid chromatography-mass spectrometry (LC-MS) was developed to achieve <u>c</u>omprehensive <u>p</u>roteome profiling of <u>u</u>rine (CPU). By this strategy, a total of 1659 proteins could be identified using a short LC gradient of <strong>∼</strong>1h, 2.3 times more than those (730) obtained from raw urine without fractionation. Compared to the current existing methods for urine sample preparation, not only the analysis time could be greatly shortened by 3–4 times, but also the identification coverage of urine proteome was increased by 130%–160%. Such a method was further combined with label-free quantification for the comparative proteome analysis of urine from IgA nephropathy (IgAN) patients and healthy donors, 227 differentially expressed proteins were identified. In addition to 90 proteins that were reported to be closely related to IgAN, we found that multiple members of solute carrier family 22 (SLC22) were up-regulated in IgAN, which might be related to the disturbance of renal metabolic function in patients. All these results demonstrated that our developed method would provide a promising tool for discovering disease related biomarkers in urine.</p></div>","PeriodicalId":75287,"journal":{"name":"Urine (Amsterdam, Netherlands)","volume":"5 ","pages":"Pages 29-33"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44379177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Urine; a new promising biological fluid to act as a non-invasive biomarker for different human diseases 尿;一种新的有前途的生物液体，可作为不同人类疾病的非侵入性生物标志物

Urine (Amsterdam, Netherlands) Pub Date : 2023-01-01 DOI: 10.1016/j.urine.2023.06.001

Namrata Balhara , Mamta Devi , Ajay Balda , Manisha Phour , Arup Giri

引用次数: 1

Changes in the urine proteome in patients with advanced lung cancer after different drug treatments 不同药物治疗后晚期肺癌患者尿蛋白质组的变化

Urine (Amsterdam, Netherlands) Pub Date : 2023-01-01 DOI: 10.1016/j.urine.2023.02.001

Yameng Zhang , Ziqi Heng , Jing Wei , Yingyi Wang , Youhe Gao

{"title":"Changes in the urine proteome in patients with advanced lung cancer after different drug treatments","authors":"Yameng Zhang , Ziqi Heng , Jing Wei , Yingyi Wang , Youhe Gao","doi":"10.1016/j.urine.2023.02.001","DOIUrl":"https://doi.org/10.1016/j.urine.2023.02.001","url":null,"abstract":"<div><p>Lung cancer is one of the most threatening diseases to human life and health because of its mortality. How to choose more efficient drugs and the appropriate timing of treatment for patients with advanced lung cancer is still a problem. As the urine proteome can sensitively reflect the pathological or physiological changes of the body, it has the potential to reflect the dynamic changes of the body after drug treatment. To investigate the changes in the urine proteome of patients with advanced lung cancer after different drug treatments, urine samples were collected and analyzed at different time points. The changes in the urine proteome from the pretreatment state were different in each patient and the changes in the biological processes were consistent with the changes in the clinical manifestations of the patients. This study demonstrates that the pathophysiological changes of patients with advanced lung cancer can be reflected by changes in urinary protein after different drug treatments. In addition, the changes in urinary proteins can reflect the different biological processes in patients after the same drug treatment, and the patients’ clinical condition assessment results are consistent with these changes. These findings may provide additional information for clinical treatment.</p></div>","PeriodicalId":75287,"journal":{"name":"Urine (Amsterdam, Netherlands)","volume":"5 ","pages":"Pages 1-12"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49896799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Urine biomarkers can outperform serum biomarkers in certain diseases 在某些疾病中，尿液生物标志物的表现优于血清生物标志物

Urine (Amsterdam, Netherlands) Pub Date : 2023-01-01 DOI: 10.1016/j.urine.2023.10.001

Cheng Xue , Bo Yang , Lili Fu , Huihui Hou , Jihua Qiang , Chenchen Zhou , Youhe Gao , Zhiguo Mao

{"title":"Urine biomarkers can outperform serum biomarkers in certain diseases","authors":"Cheng Xue , Bo Yang , Lili Fu , Huihui Hou , Jihua Qiang , Chenchen Zhou , Youhe Gao , Zhiguo Mao","doi":"10.1016/j.urine.2023.10.001","DOIUrl":"https://doi.org/10.1016/j.urine.2023.10.001","url":null,"abstract":"<div><p>Urinary biomarkers offer a non-invasive and easily accessible means of assessing an individual's health and susceptibility to various diseases. Urine biomarkers have advantages in no need or mechanism for stability, specific biomarkers produced by tubules, and non-invasive nature compared with serum biomarkers. Urine biomarkers can provide critical insights into an individual's predisposition to certain conditions, disease progression, and therapeutic response. In this review, we summarized the currently reported urinary biomarkers that outperformed serum biomarkers, including urinary protein biomarkers, gene biomarkers, urinary metabolites, electrolytes, and urinary extracellular vesicles. Combining urinary and serum biomarkers can offer a more comprehensive approach to disease diagnosis, monitoring, and personalized medicine. Despite some challenges in standardization and expanding the repertoire of diseases that can be diagnosed using urinary biomarkers, urinary biomarkers hold immense promise in improving patient outcomes and transforming healthcare.</p></div>","PeriodicalId":75287,"journal":{"name":"Urine (Amsterdam, Netherlands)","volume":"5 ","pages":"Pages 57-64"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590280623000086/pdfft?md5=bd4f7c211d784b4699f9c25680a72179&pid=1-s2.0-S2590280623000086-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92026115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Induced crystal formation in urine as an easily accessible indicator of tumor stage? – A translational explorative study 尿中诱导晶体形成作为肿瘤分期的一个容易接近的指标?-翻译探索性研究

Urine (Amsterdam, Netherlands) Pub Date : 2023-01-01 DOI: 10.1016/j.urine.2023.05.001

Norbert Laube , Florian Klein , Christian Fisang

引用次数: 0

A review on traditional natural compounds and conventional methods for the treatment of UTI 传统天然化合物和治疗尿路感染的传统方法综述

Urine (Amsterdam, Netherlands) Pub Date : 2023-01-01 DOI: 10.1016/j.urine.2023.04.001

Tannupriya, Vivek Kumar Garg

{"title":"A review on traditional natural compounds and conventional methods for the treatment of UTI","authors":"Tannupriya, Vivek Kumar Garg","doi":"10.1016/j.urine.2023.04.001","DOIUrl":"10.1016/j.urine.2023.04.001","url":null,"abstract":"<div><p>The intensity of the urinary tract infection (UTI), a recurrent infection that is common in coastal locations and that affects people of different ages, varies. One of the main bacteria that causes UTI is E<em>scherichia coli</em>. The objective of the present study is to regulate the prevalence of UTI and to evaluate the effectiveness of recent developments in alternative medicine. Quinolones and cephalosporin are well-known treatments for UTIs. The only costly, non-Indian plant source medicine is cranberry extract. The growth of opportunistic Uropathogens is facilitated by pH change in urethra that occur throughout puberty and menopause in females. Additionally adding to the defence is innate immunity. Although innate immunity can support the body's resistance, a recent study found that infection rates differ among people depending on their age, immunity, and lifestyle. The microorganisms that cause UTI are becoming more and more medication resistant. Understanding the value of enhancing innate immunity and being aware of the most effective UTI treatment plan are both necessary. Alternative, innovative methods to treatment have so raised some hope. Homeopathic, Ayurvedic, Unani, and other herbal-based medications were among the therapeutic options. Nanoparticles may play new, unproven roles in the fight against UTIs in the future. Nanotechnology techniques based on medicinal plants have produced encouraging results. Therefore, there needs to be ongoing study into herbal treatments for UTIs, like Ayurvedic Biology.</p></div>","PeriodicalId":75287,"journal":{"name":"Urine (Amsterdam, Netherlands)","volume":"5 ","pages":"Pages 13-22"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45621358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A new HPLC-MS/MS method for urinary creatinine determination: Comparison study with Jaffè’s method HPLC-MS/MS测定尿肌酐的新方法:与Jaffè方法的比较研究

Urine (Amsterdam, Netherlands) Pub Date : 2023-01-01 DOI: 10.1016/j.urine.2023.04.002

Lidia Caporossi, Enrico Paci, Silvia Capanna, Bruno Papaleo, Giovanna Tranfo, Daniela Pigini

引用次数: 1

Commentary: Why have different key biomarkers been reported in the same types of samples from patients with identical diseases? 评论：为什么在患有相同疾病的患者的相同类型样本中报告了不同的关键生物标志物？

Urine (Amsterdam, Netherlands) Pub Date : 2023-01-01 DOI: 10.1016/j.urine.2023.09.001

Takaaki Sato , Yasuhiko Takahashi , Yoichi Mizutani

{"title":"Commentary: Why have different key biomarkers been reported in the same types of samples from patients with identical diseases?","authors":"Takaaki Sato , Yasuhiko Takahashi , Yoichi Mizutani","doi":"10.1016/j.urine.2023.09.001","DOIUrl":"https://doi.org/10.1016/j.urine.2023.09.001","url":null,"abstract":"<div><p>Accurate biomarkers are crucial for early disease detection and improved prognosis. However, the inconsistent reporting of different key biomarkers in the same types of samples from patients with identical diseases in biomarker discovery studies is often questioned. In contrast to such instrumental analyses, the murine olfactory system consistently distinguishes subtle variations in genetically determined individual-unique body odors in urine samples and more pronounced differences in diet-modulated and fluctuating body odors. Interestingly, sniffer mouse behavioral assays revealed that prostate and bladder cancers alter olfactory cues in urine samples to be more intense compared with diet-modulated or genetically determined individual-specific body odors. The causes of inconsistent key biomarkers include high inter-individual and inter-sample variability due to diet-induced metabolites and cosmetic or environmental contaminations. Previously, we proposed experimental procedures tolerant to such noise-like variability or fluctuation, leading to the identification of ten urinary volatile biomarkers for prostate cancer, including 2,6-di(propan-2-yl)phenol as a unique biomarker for bladder cancer. This commentary discusses the theoretical basis of urinary volatile biomarkers and future directions for complementary biomarker development for diagnosis.</p></div>","PeriodicalId":75287,"journal":{"name":"Urine (Amsterdam, Netherlands)","volume":"5 ","pages":"Pages 53-56"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49847074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0