{"title":"Rhizosphere Bacteriobiome of the Husk Tomato Grown in the Open Field in West Siberia","authors":"N. Naumova, O. Savenkov, T. Alikina, M. Kabilov","doi":"10.2478/agri-2019-0015","DOIUrl":"https://doi.org/10.2478/agri-2019-0015","url":null,"abstract":"Abstract The composition and structure of rhizosphere bacteriobiome of the husk tomato (Physalis philadelphica Lam.) plants grown on Phaeozem in the open field in West Siberia, Russia (55°15’ NL, 83°31’ EL) were studied using Illumina MiSeq sequencing of the V3-V4 hypervariable region of 16S rRNA genes. In total 5898 OTUs (Operational Taxonomic Units) were found in the study, representing 20 phyla and 53 identified and 15 non-identified (below the phylum level) classes. The most OTU-rich phyla were Proteobacteria, Acidobacteria and Actinobacteria, their relative abundance in the total number of sequence reads being 26, 22 and 19%, respectively. Bacteroidetes, Gemmatimonadetes and Verrucomicrobia phyla each accounted for 2 ‒ 4%. The rest 14 of the identified phyla were quite negligible, contributing less than 0.5% each. At the OTUs level, the structure was very even and equitable, as only 7 OTUs had relative abundance ranging from 0.5 to 1.1%. The main dominant OTU represented Bradyrhizobiaceae family, implying the importance of nitrogen-fixing bacteria for plant growth and development without any mineral fertilisation. The dominance biodiversity index was very low (0.001), while Shannon index was rather high (7.5). We believe the presented husk tomato rhizosphere bacteriobiome, as the first study using new generation sequencing platform for this species, will help get a better picture of Solanaceae microbiomes in different environments, thus contributing to a more comprehensive understanding of shaping microbial communities by plant roots.","PeriodicalId":7527,"journal":{"name":"Agriculture (Pol'nohospodárstvo)","volume":"70 1","pages":"147 - 154"},"PeriodicalIF":0.0,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89932624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Hospodarenko, I. Prokopchuk, O. Nikitina, V. Liubych
{"title":"Assessment of the Contamination Level of a Podzolized Chernozem with Nuclides in a Long-term Land Use","authors":"H. Hospodarenko, I. Prokopchuk, O. Nikitina, V. Liubych","doi":"10.2478/agri-2019-0013","DOIUrl":"https://doi.org/10.2478/agri-2019-0013","url":null,"abstract":"Abstract Prolonged systematic application of mineral fertilisers contributes to increasing the yielding capacity of agricultural crops. However, it can lead to significant changes in the composition, properties and formation of agricultural soil regimes. The findings of the research have shown that the application of mineral fertilisers leads to the change of radioactive nuclides content in the soil. The research was conducted under conditions of a long-term stationary field experiment (Uman, Ukraine), using different rates of mineral fertilisers N45P45K45, N90P90K90 and N135P135K135. Soil samples (podzolized chernozem) were selected from the depths of 0 – 20, 20 – 40 and 40 – 60 cm. Specific activity of radionuclides was determined by the spectrometric analysis. Using experimental results we have demonstrated that under a long-term application (50 years) different rates of mineral fertilisers effect the specific activity of radioactive isotopes in the soil (226Ra, 232Th, 40K, 137Cs, 90Sr). The specific activity of radionuclides in a podzolized chernozem and in winter wheat grain was established. Winter wheat plants accumulated 232Th at the highest levels, but the use of fertilisers reduced it in a larger mass of the crop. The absorption of radioactive nuclides by winter wheat grain grown after peas and silage corn depending on fertilisation changed similar to growing it after clover as a previous crop. According to the data of specific activity of radioactive nuclides in the soil and winter wheat grain, the coefficient of their biological absorption was calculated.","PeriodicalId":7527,"journal":{"name":"Agriculture (Pol'nohospodárstvo)","volume":"10 1","pages":"128 - 135"},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89412618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Growth of Potato Shoot Cultures on Media with Antibiotics for Elimination of Bacterial Contamination","authors":"M. Gubišová, J. Gubiš","doi":"10.2478/agri-2019-0010","DOIUrl":"https://doi.org/10.2478/agri-2019-0010","url":null,"abstract":"Abstract The aim of our work was to evaluate the effect of selected antibiotics on the growth of potato shoot cultures in the Gene Bank of the Slovak Republic collection and to determine the type and dose that may be used to treat potato cultures endangered by endophytic bacteria. Antibiotics Chloramphenicol at doses 20, 50 and 100 mg/L, Gentamycin and Rifampicin in doses 20, 50, 100, 200 mg/L and the combination of Gentamycin and Rifampicin with 100 mg/L of each were used. Growth parameters – the shoot length and the number of nodal segments per shoot and rooting of ten cultivars of Solanum tuberosum L. of different origin were evaluated. Chloramphenicol already at the lowest dose had the strong inhibitory effect on regeneration, growth, and rooting of shoots. Gentamycin inhibited the growth of shoots gradually with increasing dose of it, rooting of shoots was negatively affected using the dose 50 mg/L or higher. Rifampicin up to 100 mg/L had the minimal effect on the shoots growth, rooting of shoots was not affected, but shoots were characterised by smaller or stunted leaves. Although the growth of shoots was affected, all ten genotypes used in the experiments were able to regenerate and grow at the highest dose of Rifampicin and Gentamycin. According to the results, it is highly probable that these antibiotics up to 100 mg/L or their combination would be suitable for culture preservation of the most genotypes in the gene-bank collection. On the other hand, Chloramphenicol cannot be recommended due to its strong detrimental effect on potato shoot cultures.","PeriodicalId":7527,"journal":{"name":"Agriculture (Pol'nohospodárstvo)","volume":"1 1","pages":"106 - 99"},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88233457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of Planting Methods on the Quality of Three Egyptian Rice Varieties","authors":"Nessreen N. Bassuony, J. Zsembeli","doi":"10.2478/agri-2019-0012","DOIUrl":"https://doi.org/10.2478/agri-2019-0012","url":null,"abstract":"Abstract In Egypt, the traditional transplanting method of rice planting is substituted by broadcasting and dibbling recently. This paper studies the effects and the changes occur in different rice varieties due to different methods on grain quality and amino acids of rice. Three Egyptian rice cultivars belonging to japonica, japonica/indica and indica subspecies were investigated for grain quality, protein and amino acid composition under three planting methods, i.e., broadcasting, seedling transplanting and dibbling. A strip plot design with three replications was used; the investigated three rice cultivars occupied the horizontal main plot, while three planting methods were devoted to the vertical main plot. The method of planting did not affect significantly the grain dimension, but it affected significantly the milling characters and the 1,000-grain weight. The highest milling characters and 1,000-grain weight came from transplanting, while the lowest one from the dibbling method. There was no significant difference between transplanting and broadcasting in milling characters and 1,000-grain weight. The chemical and cooking characters of grains showed no significant differences in moisture content and gelatinization temperature characters, while the differences in protein content and elongation were significant among the varieties and planting methods. The interaction between the planting method and rice had significant effect on the protein content. The most important essential amino acids according to the FAO/WHO pattern were not affected significantly by the different methods of planting. This means that the biological value of rice grains is not affected by different methods of planting.","PeriodicalId":7527,"journal":{"name":"Agriculture (Pol'nohospodárstvo)","volume":"72 1","pages":"119 - 127"},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91257546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Z. Jabbarov, Tokhtasin Abdrakhmanov, A. Pulatov, P. Kováčik, Khabibullo Pirmatov
{"title":"Change in the Parameters of Soils Contaminated by Oil and Oil Products","authors":"Z. Jabbarov, Tokhtasin Abdrakhmanov, A. Pulatov, P. Kováčik, Khabibullo Pirmatov","doi":"10.2478/agri-2019-0009","DOIUrl":"https://doi.org/10.2478/agri-2019-0009","url":null,"abstract":"Abstract The oil well drilling and oil processing industries are globally the main contaminants of environmental condition caused by human economic activities. Oil spills have a negative impact on the environment, economy, and society. In this research, the effects of oil with different chemical contents on soil types formed in two soil-climatic conditions have been studied. The purpose of this research is to study the change of soil properties by oil pollution. The experiments have been conducted in irrigated and non-irrigated soils of the desert region of the Kashkadarya and Surkhandarya (Uzbekistan). The results have shown that aggregates (0.25; 0.5; 1; 2; 3; 5; 7; 10 mm) which are the important of soil fertility have changed by oil and oil production, and the changes have proven to be temporary. The effect of the 5% and 15% concentrations of oil, engine oil, petrol, kerosene has been studied. The aggregates 0.25 mm and 0.5 mm have the biggest change among aggregates, in fact, aggregates of 0.25 mm at the level of 5% of oil decreased by 27.02%, at the level of 15% of oil decreased by 99.8%, at the level of 5% of kerosene decreased by 2%, at the level of 15% of oil decreased by 98.1%. Aggregates of 0.5 mm at the level of 5% of oil decreased by 6.44%, at the level of 15% of oil decreased by 67.14%, at the level of 5% of kerosene decreased by 12.75%, at the level of 15% of oil decreased by 92.8%. Engine oil and Petrol at levels 5 and 15 have relatively rare changed. Also, as a result of oil and oil pollution, the total carbon dioxide in the soil has grown briefly, which is an anthropogenic carbon and insignificant for soil fertility and humus. As a result, an anthropogenic carbon increased in gray-brown soil (Durisols Technic, WRB) at 0 – 35 cm layer by 0.22%, irrigated meadow-alluvial soil (Fluvisols, WRB) by 0.31%, irrigated gray-brown soil (Durisols Technic, WRB) by 0.44%, irrigated Takyr-meadow soil (Calsisols, WRB) by 0.25%, Takyr soil (Calsisols, WRB) by 0.32%, sandy Desert soil (Durisols Technic, WRB) by 0.21%.","PeriodicalId":7527,"journal":{"name":"Agriculture (Pol'nohospodárstvo)","volume":"20 1","pages":"88 - 98"},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84994298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Responses of Two Barley Subspecies to In vitro-Induced Heavy Metal Stress: Seeds Germination, Seedlings Growth and Cytotoxicity Assay","authors":"M. Nouri, Taoufik El Rasafi, A. Haddioui","doi":"10.2478/agri-2019-0011","DOIUrl":"https://doi.org/10.2478/agri-2019-0011","url":null,"abstract":"Abstract In this work three heavy metals: cadmium (as CdSO4), cobalt (as CoCl2) and zinc (as ZnSO4), were used to determine and compare their toxicity towards two subspecies of barley (Hordeum vulgare subsp. vulgare L. and Hordeum vulgare subsp. distichum L.), focusing on seeds germination, seedlings growth, and cytological parameters. The results indicate that the effect of these heavy metals depends on the metal kind, the metal concentrations and the plant subspecies. Generally, in the case of H. vulgare, the heavy metal salts understudy did not influence significantly seed germination and seedling growth parameters. However, these metal salts influence significantly these parameters for H. distichum. The cytological test showed significant decrease (p < 0.05) in the mitotic index among the increase of the heavy metal concentrations when evaluated with the control for H. vulgare and H. distichum. Consequently, H. vulgare seemed to be more tolerant of the increase of the three heavy metals concentrations than H. distichum.","PeriodicalId":7527,"journal":{"name":"Agriculture (Pol'nohospodárstvo)","volume":"1 1","pages":"107 - 118"},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83491943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Genotype by Environment Interaction for Grain Yield of Barley Mutant Lines","authors":"B. Dyulgerova, N. Dyulgerov","doi":"10.2478/agri-2019-0006","DOIUrl":"https://doi.org/10.2478/agri-2019-0006","url":null,"abstract":"Abstract The aim of this study was to examine the genotype by environment interaction for grain yield and to identify high-yielding and stable mutant lines of 6-rowed winter barley under different growing seasons. The study was carried out during 7 growing seasons from 2010 – 2011 to 2016 – 2017 in the experimental field of the Institute of Agriculture – Karnobat, Southeastern Bulgaria. Fourteen advanced mutant lines and the check variety Vesletc were studied using a complete block design with 4 replications. The AMMI analysis of variance indicated that 20.54% of the variation for grain yield was explained by the effect of genotype and 37.34% and 42.12% were attributable to the environmental effects and genotype by environment interaction. The magnitude of the genotype by environment interaction was two times larger than that of genotypes, indicating that there was a substantial difference in genotype response across environments. The AMMI and GGE biplot analyses identified G9 as the highest yielding and stable genotype. This mutant line can be recommended for further evaluation for variety release. The mutant lines G6, G13 and G15 were suggested for inclusion in the breeding program of winter barley due to its high grain yield and intermediate stability.","PeriodicalId":7527,"journal":{"name":"Agriculture (Pol'nohospodárstvo)","volume":"29 1","pages":"51 - 58"},"PeriodicalIF":0.0,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86925866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. El-Mogy, A. Salama, Hanaa F. Y. Mohamed, Karima F. Abdelgawad, Emad A. Abdeldaym
{"title":"Responding of Long Green Pepper Plants to Different Sources of Foliar Potassium Fertiliser","authors":"M. El-Mogy, A. Salama, Hanaa F. Y. Mohamed, Karima F. Abdelgawad, Emad A. Abdeldaym","doi":"10.2478/agri-2019-0007","DOIUrl":"https://doi.org/10.2478/agri-2019-0007","url":null,"abstract":"Abstract The aim of this study was to explore the efficiency of foliar potassium fertiliser relative to morphological, physiological and biochemical properties of hybrid long pepper (Capsicum annuum). Pepper plants were grown in a greenhouse and supplied with three sources of foliar potassium fertilisers, i.e., potassium-humate (1 g/L = 1,250 g/ha), potassium sulfate (1 g/L = 1,250 g/ha), and potassium chloride (1 g/L = 1,250 g/ha). Water served as control. The impacts of these treatments on the phytosynthetic parameters (photosynthetic rate, stomatal conductance, intercellular CO2, leaf carotenoids) and chlorophyll a and b, metabolic compounds and nitrogen, phosphorus and potassium were measured. The phytosynthetic parameters significantly improved by different foliar potassium application and the highest level of photosynthetic activity was noted in plants supplied with potassium sulfate, followed by potassium-humate and potassium chloride. Plant biomass accumulation, cholorophyll (a and b), and total yield showed larger increases in plants fertilised with potassium sulfate than those fertilised with potassium-humate; smallest increases occurred with potassium chloride. Concentrations of total sugars, carotenoids, chlorophyll (a and b), and endogenous level of nitrogen, phosphorus, and potassium contents in plants and fruits were possitively influenced by varying sources of potassium. The fruit color parameters and total soluble solid were also significantly increased with all foliar potassium treatments compared with control. Foliar application of potassium sulfate recorded the highest values and significantly increase all anatomical characters for leaf, stem and fruit of pepper plant.","PeriodicalId":7527,"journal":{"name":"Agriculture (Pol'nohospodárstvo)","volume":"29 1","pages":"59 - 76"},"PeriodicalIF":0.0,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83580126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Characterization and Evaluation of Bacillus siamensis Isolate for its Growth Promoting Potential in Tomato","authors":"Amanul Islam, S. Kabir, A. Khair","doi":"10.2478/agri-2019-0005","DOIUrl":"https://doi.org/10.2478/agri-2019-0005","url":null,"abstract":"Abstract In quest of prospective rizospheric bacteria of agricultural importance, one of the isolates from bean (Lablab niger Medikus) was identified as Bacillus siamensis based on morphological, biochemical and 16S rRNA gene sequencing data. Study was carried out to evaluate growth promotion of two tomato cultivars, in vitro and in vivo. Experiments conducted for two consecutive years, following seed treatments revealed that the bacterial isolate increased plant height by 14.66‒15.68%, shoot fresh weight by 34.5‒65.09% and root fresh weight by 75.3‒92.48% over the non-treated control. The bacterial strain showed encouraging results for plant growth promotion in pot study and hence may be useful for the growth enhancement of tomato plant.","PeriodicalId":7527,"journal":{"name":"Agriculture (Pol'nohospodárstvo)","volume":"24 1","pages":"42 - 50"},"PeriodicalIF":0.0,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76600857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Zsembeli, Lúcia Sinka, Arzu Rivera-García, K. Czellér, Géza Tuba, K. Krištof, P. Findura
{"title":"Effect of Soil Conditioning on the Moisture Content and the Salt Profile of the Soil Under Irrigation with Saline Water","authors":"J. Zsembeli, Lúcia Sinka, Arzu Rivera-García, K. Czellér, Géza Tuba, K. Krištof, P. Findura","doi":"10.2478/agri-2019-0008","DOIUrl":"https://doi.org/10.2478/agri-2019-0008","url":null,"abstract":"Abstract The research work was started on the preliminary knowledge that the risk of secondary salinization is high in the hobby gardens around Karcag as the water of the aquifers used for irrigation is saline, nevertheless irrigation in the drought periods is essential for vegetable production. A complex experiment was set up in 12 simple drainage lysimeters at the lysimeter station of the Research Institute of Karcag in 2012 in order to simulate the conditions of irrigation characteristic in the region with the goal of finding a solution to mitigate the harmful effects by means of optimization of irrigation. In 2017–2018 three approaches were applied for the scientific establishment of the problem studying the effect of different irrigation frequencies, different irrigation water qualities, and soil conditioning on the moisture content and the salt profile of the soil. The soil conditioner (Neosol) applied was found to have a positive effect on the water and salt regime of the soil, partly by creating a more favourable vertical distribution of the soil water, and partly preserving more moisture in the soil.","PeriodicalId":7527,"journal":{"name":"Agriculture (Pol'nohospodárstvo)","volume":"1 1","pages":"77 - 87"},"PeriodicalIF":0.0,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82488087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}