Agriculture (Pol'nohospodárstvo)最新文献

筛选
英文 中文
Winter Barley Production in Relation to Crop Rotations, Fertilisation and Weather Conditions 冬大麦生产与轮作、施肥和天气条件的关系
Agriculture (Pol'nohospodárstvo) Pub Date : 2018-04-01 DOI: 10.2478/agri-2018-0004
M. Babulicová, B. Dyulgerova
{"title":"Winter Barley Production in Relation to Crop Rotations, Fertilisation and Weather Conditions","authors":"M. Babulicová, B. Dyulgerova","doi":"10.2478/agri-2018-0004","DOIUrl":"https://doi.org/10.2478/agri-2018-0004","url":null,"abstract":"Abstract The aim of the study was to evaluate the influence of crop rotations, fertilisation and weather conditions on the grain yield and yield components of winter barley. Long-term field experiment was situated in the area of continental climate. There were the crop rotations with 40%, 60% and 80% share of the cereals. Two treatments of fertilisation were applied: H1 and H2. In the first treatment (H1), nitrogen, phosphorus and potassium in mineral fertilisers + organic manure Veget® were applied. In the second treatment (H2), nitrogen, phosphorus and potassium were applied in fertilisers only. Winter barley cultivar Barcelona was grown in years 2010-2015. The number of spikes per 1 m2, proportion of grains over sieve 2.5 + 2.8 mm and grain yield of winter barley were statistically significantly influenced by fertilisation. In the variant H1 (mineral fertilisation with addition of organic manure Veget®), the statistically higher (P ≤ 0.05) grain yield was obtained (5.71 t/ha) than in the treatment with mineral fertilisation only (5.30 t/ha). In the year 2011, which was very unfavourable for winter barley development, the grain yield in the treatment with mineral fertilisation with the addition of organic manure Veget® was by 1.23 t/ha (32.1%) higher than that in treatment with mineral fertilisa tion only.","PeriodicalId":7527,"journal":{"name":"Agriculture (Pol'nohospodárstvo)","volume":"12 1","pages":"35 - 44"},"PeriodicalIF":0.0,"publicationDate":"2018-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79127884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
The In Silico Predicton of the Chloroplast Maturase K Gene Polymorphism in Several Barley Varieties 几种大麦品种叶绿体成熟酶K基因多态性的计算机预测
Agriculture (Pol'nohospodárstvo) Pub Date : 2018-04-01 DOI: 10.2478/agri-2018-0001
K. Mustafa, Mufeed J. Ewadh, M. Al-Shuhaib, H. Hasan
{"title":"The In Silico Predicton of the Chloroplast Maturase K Gene Polymorphism in Several Barley Varieties","authors":"K. Mustafa, Mufeed J. Ewadh, M. Al-Shuhaib, H. Hasan","doi":"10.2478/agri-2018-0001","DOIUrl":"https://doi.org/10.2478/agri-2018-0001","url":null,"abstract":"Abstract This study was conducted to describe the role of the chloroplast maturase K (matK) genetic polymorphism in the reciprocal crossing between five barley varieties using several in vitro / in silico tools. Besides, the final consequences of the matK gene polymorphism on its protein structure, function, and interactions were predicted computationally. Five parental varieties were crossed to each other by full reciprocal crossing design, DNA was extracted from seeds and two different primers’ pairs were designed to scan matK gene. Then, polymerase chain reaction - single-stranded conformation polymorphism (PCR-SSCP) were performed. Two distinct haplotypes in both parents and artificial F1 hybrids in the matK gene were observed in both amplified fragments. This finding indicated that the studied gene had no participation in the reciprocal crossing performed. Three SNPs were identified; two of them are non-synonymous (nsSNPs), namely G387V and L459M. The effect of these missense mutations on the matK protein was analyzed by several in silico tools. It was shown that the coding SNP, L459M was predicted to have much more effective consequences on matK protein structure and function. While the I-Mutant 2.0 prediction tool showed a decrease in stability for these two nsSNPs, which may destabilize the protein interactions to some extent. In conclusion, though the observed missense mutations in the matK gene have no suggestive role in the reciprocally crossed barley varieties, they caused dramatic alterations in several matK protein moieties, which may lead to potential subsequent changes in the matK protein-mediated RNA splicing mechanisms.","PeriodicalId":7527,"journal":{"name":"Agriculture (Pol'nohospodárstvo)","volume":"26 1","pages":"16 - 3"},"PeriodicalIF":0.0,"publicationDate":"2018-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74781785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
The Effect of Different Tillage Methods on Erosion 不同耕作方式对土壤侵蚀的影响
Agriculture (Pol'nohospodárstvo) Pub Date : 2018-04-01 DOI: 10.2478/agri-2018-0003
M. Vach, L. Hlisnikovský, M. Javůrek
{"title":"The Effect of Different Tillage Methods on Erosion","authors":"M. Vach, L. Hlisnikovský, M. Javůrek","doi":"10.2478/agri-2018-0003","DOIUrl":"https://doi.org/10.2478/agri-2018-0003","url":null,"abstract":"Abstract During the years 2012‒2016 at the site threatened by erosion, the effect of different intensity and depth of soil tillage on the progression of erosion were evaluated on the plots with silage maize. Three different tillage methods were compared and evaluated - conventional tillage, including ploughing (CT), no-tillage using mulch and direct drilling (NT), and minimum tillage treatment with a lower depth of soil cultivation and organic matter incorporation (MT). Water and soil runoff on all of the experimental plots were measured during erosion events. Besides an analysis of naturally occurring rainfall causing erosions, we also conducted the test of soil infiltration abilities with a rain simulator after silage maize harvest. The effect of the tillage on aboveground biomass yield and the input costs was also analyzed. The results showed that NT and MT can significantly reduce water and soil runoff comparing CT. The highest yields were recorded in MT, while the lowest were in CT. Total input costs were higher in the case of NT and MT, but the share of mechanized work was lower for these technologies. Our results showed that NT and MT technologies, as a part of silage maize with a higher plant density stand establishment, should be a useable erosion control measure in areas vulnerable to erosion.","PeriodicalId":7527,"journal":{"name":"Agriculture (Pol'nohospodárstvo)","volume":"103 1","pages":"28 - 34"},"PeriodicalIF":0.0,"publicationDate":"2018-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91336431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Phenotyping Root System Architecture of Cotton (Gossypium barbadense L.) Grown Under Salinity 棉花根系结构的表型分析盐度下生长
Agriculture (Pol'nohospodárstvo) Pub Date : 2017-12-01 DOI: 10.1515/agri-2017-0014
S. A. Mottaleb, E. Darwish, M. Mostafa, G. Safwat
{"title":"Phenotyping Root System Architecture of Cotton (Gossypium barbadense L.) Grown Under Salinity","authors":"S. A. Mottaleb, E. Darwish, M. Mostafa, G. Safwat","doi":"10.1515/agri-2017-0014","DOIUrl":"https://doi.org/10.1515/agri-2017-0014","url":null,"abstract":"Abstract Soil salinity causes an annual deep negative impact to the global agricultural economy. In this study, the effects of salinity on early seedling physiology of two Egyptian cotton (Gossypium barbadense L.) cultivars differing in their salinity tolerance were examined. Also the potential use of a low cost mini-rhizotron system to measure variation in root system architecture (RSA) traits existing in both cultivars was assessed. Salt tolerant cotton cultivar ‘Giza 90’ produced significantly higher root and shoot biomass, accumulated lower Na+/K+ ratio through a higher Na+ exclusion from both roots and leaves as well as synthesized higher proline contents compared to salt sensitive ‘Giza 45’ cultivar. Measuring RSA in mini-rhizotrons containing solid MS nutrient medium as substrate proved to be more precise and efficient than peat moss/sand mixture. We report superior values of main root growth rate, total root system size, main root length, higher number of lateral roots and average lateral root length in ‘Giza 90’ under salinity. Higher lateral root density and length together with higher root tissue tolerance of Na+ ions in ‘Giza 90’ give it an advantage to be used as donor genotype for desirable root traits to other elite cultivars.","PeriodicalId":7527,"journal":{"name":"Agriculture (Pol'nohospodárstvo)","volume":"19 1","pages":"142 - 150"},"PeriodicalIF":0.0,"publicationDate":"2017-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85228289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
The Effects of Foliar Feeding of Compatible Organic Solutes on Agronomic Traits of Safflower 叶面补施有机溶质对红花农艺性状的影响
Agriculture (Pol'nohospodárstvo) Pub Date : 2017-12-01 DOI: 10.1515/agri-2017-0013
M. Janmohammadi, Farzaneh Asadi, N. Sabaghnia, A. Abbasi, M. Nouraein, F. Shekari
{"title":"The Effects of Foliar Feeding of Compatible Organic Solutes on Agronomic Traits of Safflower","authors":"M. Janmohammadi, Farzaneh Asadi, N. Sabaghnia, A. Abbasi, M. Nouraein, F. Shekari","doi":"10.1515/agri-2017-0013","DOIUrl":"https://doi.org/10.1515/agri-2017-0013","url":null,"abstract":"Abstract Safflower is originated from Iran and is tolerant against water deficit stress. However, in semi-arid Mediterranean climate terminal drought and heat stress adversely affect the safflower production. In order to investigate the influence of foliar application of proline (Pr) (10 and 20 mM) and glycinebetaine (GB) (2 and 4 mM) under well and deficit irrigation (37.23° N,46.16° E). Foliar spray of compatible organic solutes started from middle vegetative growth and continued till seed filling stage. Comparison of well irrigated and stress conditions revealed that severity of water deficit stress (SI) was 0.25. Evaluation of growth-related morphological characteristics such as plant height, leaf area, canopy spread and percent ground cover showed that they considerably reduced by water deficit stress. However, foliar application of compatible solutes could somewhat increase growth related parameters. Results showed that water deficit stress noticeably reduced the chlorophyll content, while foliar spray could alleviate the water deficit stress effects when compared with intact plant (non-sprayed plants). The beneficial effect of GB was more prominent than Pr, especially under deficit irrigation condition. Principal component analysis (PCA) indicated that the best performance under well irrigated condition was obtained by application of 4 mM GB while under deficit irrigation condition the best performance was recorded for plants treated with 2 and 4 mM GB and 20 mM Pr. Overall, results of current experiments showed that foliar spray with high concentration of GB may can significantly alleviate the adverse effects of water deficit stress.","PeriodicalId":7527,"journal":{"name":"Agriculture (Pol'nohospodárstvo)","volume":"373 1","pages":"128 - 141"},"PeriodicalIF":0.0,"publicationDate":"2017-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76653043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Uptake and Translocation of Some Heavy Metals by Rice Crop (Oryza sativa) in Paddy Soils 水稻土壤中某些重金属的吸收和转运
Agriculture (Pol'nohospodárstvo) Pub Date : 2017-12-01 DOI: 10.1515/agri-2017-0016
G. Rahimi, Z. Kolahchi, A. Charkhabi
{"title":"Uptake and Translocation of Some Heavy Metals by Rice Crop (Oryza sativa) in Paddy Soils","authors":"G. Rahimi, Z. Kolahchi, A. Charkhabi","doi":"10.1515/agri-2017-0016","DOIUrl":"https://doi.org/10.1515/agri-2017-0016","url":null,"abstract":"Abstract Accumulation of heavy metals in edible crops is amongst major international concerns today. While consuming Lenjan variety of rice is very popular in Iran, limited evidence exists on its safety. Amid increasing public concern about the safety of locally grown and imported rice in the market, a field study was carried out to investigate uptake and translocation of Cd, Pb, Ni, and Zn by a local variety of rice crop (Oryza sativa) exposed to contaminated water. At harvest time and in paddy fields, 41 soil and plant samples were collected from four locations of Lenjan, central Iran; irrigated from Zayandeh Rood River. In the laboratory, different parts of the plant were milled, digested via acid digestion method, and then analysed for Cd, Pb, Ni and Zn using atomic absorption spectrophotometry. The results showed that average concentrations of Cd, Pb, Ni and Zn were 1.07, 17.22, 1.73 and 13.75 mg/kg in the plant’s stem; and 1.27, 12.32, 1.099 and 19.39 mg/kg in its grain, respectively. In general, both in the plant’s stem and grain, the Cd and Pb concentrations were much higher than the FAO/WHO standard and labelled as harmful for consumers. Moreover, among the studied heavy metals, Ni transported very weakly, while Cd and Zn conveyed most easily into the plant’s stem and grain. Of course, Pb was the least mobile metal. However, it had highly accumulated in the plant’s stem and grain.","PeriodicalId":7527,"journal":{"name":"Agriculture (Pol'nohospodárstvo)","volume":"1 1","pages":"163 - 175"},"PeriodicalIF":0.0,"publicationDate":"2017-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89933243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 26
Soil Organic-Matter in Water-Stable Aggregates Under Different Soil-Management Practices 不同土壤管理方式下土壤水稳性团聚体有机质
Agriculture (Pol'nohospodárstvo) Pub Date : 2017-12-01 DOI: 10.1515/agri-2017-0015
V. Šimanský, J. Horák, B. Clothier, N. Buchkina, D. Igaz
{"title":"Soil Organic-Matter in Water-Stable Aggregates Under Different Soil-Management Practices","authors":"V. Šimanský, J. Horák, B. Clothier, N. Buchkina, D. Igaz","doi":"10.1515/agri-2017-0015","DOIUrl":"https://doi.org/10.1515/agri-2017-0015","url":null,"abstract":"Abstract An experiment of different management practices in a commercial vineyard, which was established in 2006 in the locality of Nitra-Dražovce, Slovakia on Rendzic Leptosol, was used to evaluate the dynamics of soil organic-matter parameters during the years 2008–2015. The following treatments were established: 1. G (grass without fertilisation as control), 2. T (tillage), 3. T+FYM (tillage + farmyard manure), 4. G+NPK3 (grass + 3rd intensity of fertilisation for vineyards: it means 125 kg/ha N, 50 kg/ha P, 185 kg/ha K), and 5. G+NPK1 (grass + 1st intensity of fertilisation for vineyards: it means 100 kg/ha N, 30 kg/ha P, 120 kg/ha K). The results showed that the soil-management practices in the vineyard significantly influenced the soil organic carbon in water-stable aggregates (SOC in WSA). The content of SOC in WSAma increased on average in the following order: T < G < G+NPK1 < G+NPK3 < T+FYM. Intensive soil cultivation in the T treatment resulted in a statistically significant build-up of SOC in WSAma at an average rate of 1.33, 1.18, 0.97, 1.22 and 0.76 g/kg/y across the size fractions > 5 mm, 5‒3 mm, 2–1 mm, 1–0.5 mm and 0.5–0.25 mm, respectively. The content of non-labile carbon reflected the contents of SOC in WSA. The highest labile carbon (CL) in WSAma, as compared to others, was found in T+FYM. Overall, application of higher NPK doses resulted in higher content of CL in WSAma compared with the lower applications of NPK. On the other hand, lower applications of NPK to soil increased the content of CL in WSAmi, as compared to G+NPK3.","PeriodicalId":7527,"journal":{"name":"Agriculture (Pol'nohospodárstvo)","volume":"1 1","pages":"151 - 162"},"PeriodicalIF":0.0,"publicationDate":"2017-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89189924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Variability of quantitative and qualitative traits of coloured winter wheat 彩色冬小麦数量性状和质量性状的变异
Agriculture (Pol'nohospodárstvo) Pub Date : 2017-10-01 DOI: 10.1515/agri-2017-0010
A. Žofajová, M. Havrlentová, M. Ondrejovič, Maroš Juraška, B. Michalíková, Ľubomíra Deáková
{"title":"Variability of quantitative and qualitative traits of coloured winter wheat","authors":"A. Žofajová, M. Havrlentová, M. Ondrejovič, Maroš Juraška, B. Michalíková, Ľubomíra Deáková","doi":"10.1515/agri-2017-0010","DOIUrl":"https://doi.org/10.1515/agri-2017-0010","url":null,"abstract":"Abstract The aim of research was to analyse winter wheat of different grain colour and to compare newly bred coloured genotypes from our breeding in grain yield and technological and nutritional quality. The set of seven purple, five blue and four yellow wheats of different origin, including seven newly bred genotypes from Vígľaš-Pstruša, was evaluated in the field experiments established by randomised complete block design in two replications in Piešťany, in the vegetations 2012/13 and 2013/14. In seven wheat varieties differing in grain colours (selected after two of each colour plus control red variety Ilona) anthocyanin composition was evaluated by HPLC analysis. Significant differences were between growing years and among colour groups in most analysed traits. Blue grain newly bred K 3575 699/3 showed the highest anthocyanin content (by 33.5% higher compared to blue grain registered variety Scorpion). However, blue grain genotypes showed negative agronomic traits combined with low number and grain weight per spike and high plant height. In new purple variety PS Karkulka, declared grain yield and its quality were confirmed and the highest mineral content (Fe, Zn, Cu, Mn) was found in selected set. Purple grain newly bred PS 5711 had lower anthocyanin content (by 17.7%), but in quality it was comparable to PS Karkulka. Varieties with yellow endosperm showed the highest number and weight of grains per spike, however it was significantly lower to Ilona. The breeding goal of coloured winter wheat is still to improve the grain yield as well as additional agronomics traits.","PeriodicalId":7527,"journal":{"name":"Agriculture (Pol'nohospodárstvo)","volume":"81 1","pages":"102 - 111"},"PeriodicalIF":0.0,"publicationDate":"2017-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86850544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Impact of operating temperature of gas transit pipeline on soil quality and production potential of crops 输气管道运行温度对土壤质量和作物生产潜力的影响
Agriculture (Pol'nohospodárstvo) Pub Date : 2017-10-01 DOI: 10.1515/agri-2017-0012
D. Halmová, Z. Poláková, L. Končeková, A. Fehér
{"title":"Impact of operating temperature of gas transit pipeline on soil quality and production potential of crops","authors":"D. Halmová, Z. Poláková, L. Končeková, A. Fehér","doi":"10.1515/agri-2017-0012","DOIUrl":"https://doi.org/10.1515/agri-2017-0012","url":null,"abstract":"Abstract The aim of this study is to investigate the effects of gas transit pipeline temperature on soil moisture, soil temperature and yield of harvest crops. The study area was located in the village Ivanka pri Nitre (Nitra District, Southwestern Slovakia). Soil type in the site is Orthic Brown Chernozem. Temperature of the transported gas increased the soil temperature in the range of 2.07°C to 3.4°C measured in a depth ranging from 250 mm to 350 mm above the gas lines. The temperature also reduced soil moisture by 1.27‒3.18 percentiles of weight. Yield of the winter wheat grown above the gas lines was higher by 9.40% in 2004 and by 13.06% in 2006. Yield of the sunflower grown above the gas lines was higher by 8.05% in 2005. In treatment 1, organic fertilisation in a dose of 50 t/ha affected the yield of the winter wheat above the gas pipeline and the yield increased by 13.95% in 2004.","PeriodicalId":7527,"journal":{"name":"Agriculture (Pol'nohospodárstvo)","volume":"125 1","pages":"120 - 127"},"PeriodicalIF":0.0,"publicationDate":"2017-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73911352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecular detection of ‘Candidatus Phytoplasma australasia’ and ‘Ca. P. cynodontis’ in Iraq “澳洲植物候选菌”和“Ca.”的分子检测。伊拉克的犬齿假体
Agriculture (Pol'nohospodárstvo) Pub Date : 2017-10-01 DOI: 10.1515/agri-2017-0011
N. Alkuwaiti, T. A. Kareem, L. J. Sabier
{"title":"Molecular detection of ‘Candidatus Phytoplasma australasia’ and ‘Ca. P. cynodontis’ in Iraq","authors":"N. Alkuwaiti, T. A. Kareem, L. J. Sabier","doi":"10.1515/agri-2017-0011","DOIUrl":"https://doi.org/10.1515/agri-2017-0011","url":null,"abstract":"Abstract The association of phytoplasma was investigated in symptomatic tomato (Solanum lycopersicum L.), eggplant (Solanum melongen L.), mallow (Malva spp.) and Bermuda grass (Cynodon dactylon L.) plants exhibiting witches’ broom and white leaf diseases, respectively. Total DNA was extracted from tomato (n=3), eggplant (n=2), mallow (n=2) and Bermuda grass (n=8) samples. Direct polymerase chain reaction (PCR) was performed using P1/P7 primer set, then PCR products were sequenced. Sequences obtained from tomato, eggplant and mallow shared 99% maximum nucleotide identity with phytoplasma belonging to subgroup 16SrII-D, and resulted therefore ‘Candidatus Phytoplasma australasia’-related. Sequences obtained from Bermuda grass showed 100% maximum nucleotide identity to 16SrXIV-A subgroup and were ‘Ca. P. cynodontis’-related. The study presents the first molecular confirmation and sequence data of presence of ‘Ca. P. australasia’ and ‘Ca. P. cynodontis’ in Iraq.","PeriodicalId":7527,"journal":{"name":"Agriculture (Pol'nohospodárstvo)","volume":"68 1","pages":"112 - 119"},"PeriodicalIF":0.0,"publicationDate":"2017-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86070578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信