The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)最新文献

{"title":"Sappanone A Aggrandises Ionising Radiation-induced Damage in Oral Epithelial Cells.","authors":"Xue Feng Zhao,&nbsp;Zhi Yong Wang,&nbsp;Xue Mei Qiu,&nbsp;Hang Zhao,&nbsp;Rui Liu,&nbsp;Qian Ming Chen","doi":"10.3290/j.cjdr.b3628117","DOIUrl":"https://doi.org/10.3290/j.cjdr.b3628117","url":null,"abstract":"<p><strong>Objective: </strong>To analyse the role played by Sappanone A, a bioactive ingredient isolated from the heartwood of Caesalpinia sappan, in the regulation of oral epithelial cell viability under radiation.</p><p><strong>Methods: </strong>Cell viability of human oral keratinocytes (HOKs) and mouse salivary gland cells under ionising radiation was analysed. Expression of Ki67 was measured by immunohistochemical staining. Fragmentation of deoxyribonucleic acid (DNA) was measured by comet assay. Cell death was analysed using trypan blue exclusion assay. Cell viability was measured using a Cell Counting Kit 8 (CCK8; Abcam, Cambridge, UK) assay.</p><p><strong>Results: </strong>Sappanone A decreased cell viability of HOK cells and mouse salivary gland cells under ionising radiation. In addition, Sappanone A enhanced radiation-induced genomic DNA fragmentation, accompanied by impaired homologous recombination and non-homologous end joining DNA repair. Mechanistic evaluation revealed that Sappanone A counteracted radiation-induced inosine monophosphate dehydrogenase 2 (IMPDH2) activation, and that this effect could be abolished by reconstituted expression of a Sappanone A-binding defective IMPDH2 mutant.</p><p><strong>Conclusion: </strong>The present study highlights a novel role played by Sappanone A in the modulation of radiosensitivity of oral epithelial cells.</p>","PeriodicalId":74983,"journal":{"name":"The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)","volume":"25 4","pages":"261-267"},"PeriodicalIF":0.0,"publicationDate":"2022-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10687532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MiR-146a-5p Promotes Dental Stem Cells Osteo/odontogenic Differentiation through NF-Kappa B Signaling Pathway by Targeting TRAF6.","authors":"Xin Yu,&nbsp;Jian Feng Lu,&nbsp;Mei Qin Gao,&nbsp;Bin Xiong,&nbsp;Wen Qian Xia","doi":"10.3290/j.cjdr.b3628171","DOIUrl":"https://doi.org/10.3290/j.cjdr.b3628171","url":null,"abstract":"<p><strong>Objective: </strong>To screen miRNAs that could simultaneously regulate osteo/odontogenic differentiation of multiple stem cells, including dental pulp stem cells (DPSCs), stem cells from the apical papilla (SCAPs) and periodontal ligament stem cells (PDLSCs).</p><p><strong>Methods: </strong>Differentially expressed miRNAs analysis on three miRNA microarrays data of dental stem cells undergoing osteo/odontogenic differentiation (GSE138180, GSE154466 and GSE159508) was performed, and miR-146a-5p were identified by bioinformatic prediction, dual-luciferase reporter assay and quantitative real-time polymerase chain reaction (PCR). In addition, differentially expressed genes between miR-146a-5p overexpressed group and control group (GSE79341) were applied for KEGG pathways enrichment analysis.</p><p><strong>Results: </strong>MiR-146a-5p expression increased in the osteo/odontogenic differentiation of DPSCs, SCAPs and PDLSCs. Tumour necrosis factor receptor (TNFR)-associated factor 6 (TRAF6) was identified as the target gene of miR-146a-5p. Furthermore, miR-146a-5p could influence the NF-Kappa B signalling pathway.</p><p><strong>Conclusion: </strong>This study suggests that miR-146a-5p could promote differentiation in multiple dental stem cells through the NF-Kappa B signalling pathway by targeting TRAF6.</p>","PeriodicalId":74983,"journal":{"name":"The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)","volume":"25 4","pages":"269-275"},"PeriodicalIF":0.0,"publicationDate":"2022-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10345284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Orthodontic and Orthognathic Treatment Combined withSurgically Assisted Rapid Maxillary Expansion in an Adult Patient with a Hyperdivergent Skeletal Pattern.","authors":"Yi Neng Han,&nbsp;Hao Liu,&nbsp;Zi Li Li,&nbsp;Yi Ping Huang,&nbsp;Wei Ran Li","doi":"10.3290/j.cjdr.b3628219","DOIUrl":"https://doi.org/10.3290/j.cjdr.b3628219","url":null,"abstract":"<p><p>In this case study, we report the successful treatment of a 35-year-old woman with a hyperdivergent skeletal pattern, open bite and severe transverse deficiency, exhibiting a skeletal Class III malocclusion. The treatment plan included 3D correction of these issues with surgically assisted rapid maxillary expansion (SARME) to solve the transverse deficiency, presurgical orthodontic treatment including aligning and levelling of the teeth in both arches, LeFort I osteotomy and bilateral sagittal split ramus osteotomy, and postsurgical correction of malocclusion. Orthodontic treatment was performed with labial brackets, and the patient achieved satisfactory occlusion and a significantly improved facial profile. Retention at the 1-year follow-up showed stable occlusion and arch forms with a harmonious facial profile.</p>","PeriodicalId":74983,"journal":{"name":"The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)","volume":"25 4","pages":"301-310"},"PeriodicalIF":0.0,"publicationDate":"2022-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10712347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comments on \"Sappanone A Aggrandises Ionising Radiation-induced Damage in Oral Epithelial Cells\".","authors":"De Meng Chen","doi":"10.3290/j.cjdr.b3628123","DOIUrl":"https://doi.org/10.3290/j.cjdr.b3628123","url":null,"abstract":"Dr Chen is currently a researcher at the Centre for Translational Medicine of the First Affiliated Hospital, Sun Yat-sen University. Using lineage tracing, gene knockout, single-cell sequencing, two-photon microscope live imaging and other scientific methods, his research is dedicated to the behaviour and regulation of stem cells in normal and malignant tissues. His work has been published in journals including Cell Stem Cell, Development, Molecular Therapy, Bone Research and Cancer Communications. 1 Centre for Translational Medicine, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, P.R. China","PeriodicalId":74983,"journal":{"name":"The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)","volume":"25 4","pages":"268"},"PeriodicalIF":0.0,"publicationDate":"2022-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10687533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Status Quo and Advanced Progress in Oral Health Care and Treatment of Children with Autism Spectrum Disorder: A Literature Review.","authors":"Lu Gao,&nbsp;Xue Nan Liu","doi":"10.3290/j.cjdr.b3628105","DOIUrl":"https://doi.org/10.3290/j.cjdr.b3628105","url":null,"abstract":"<p><p>Autism spectrum disorder (ASD) has become one of the fastest growing diseases in the world, causing a great burden to ASD children's families and society. Children with ASD face more disadvantages relating to their oral health than those without ASD. There is a positive correlation between prevalence of caries lesions and severity of ASD. Poorer oral hygiene, higher detection rates of dental calculus and far more frequent cases of gingivitis occur in children with ASD. Traumatic injuries and various types of malocclusions are more frequent in children with ASD. Poorer oral health care and treatment status are caused by multiple adverse factors. Ways of promoting effective oral health care and treatment include pretreatment counselling; improvement of the individualised treatment environment; routine behaviour guidance techniques (BGTs) including tell-show-do, distraction, role model presentation, voice control, visual education and social stories, encouragement and reinforcement; targeted BGTs including visual education, behaviour modelling, applied behaviour analysis (ABA) and systematic desensitisation; passive BGTs including protective restraint, pharmaceutically administrated sedation and general anaesthesia; oral health education for guardians; and interdisciplinary collaboration and professional dental care/treatment. Dentists, families with children with ASD and schools should cooperate to improve family-centred oral health care and treatment for ASD children not only in China, but also the whole world.</p>","PeriodicalId":74983,"journal":{"name":"The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)","volume":"25 4","pages":"251-259"},"PeriodicalIF":0.0,"publicationDate":"2022-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10527977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expressions of GLUT-1, PK-M2 and HIF-1α and Mutation Status of BRAF in Odontogenic Keratocysts.","authors":"Zhu You,&nbsp;Jing Du,&nbsp;Li Li Xu,&nbsp;He Yu Zhang,&nbsp;Xue Fen Li,&nbsp;Zhi Peng Sun,&nbsp;Li Sha Sun","doi":"10.3290/j.cjdr.b3628195","DOIUrl":"https://doi.org/10.3290/j.cjdr.b3628195","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the expressions and clinicopathological features of glucose transporter 1 (GLUT-1), pyruvate kinase M2 (PK-M2) and hypoxia-inducible factor 1α (HIF-1α) in odontogenic keratocysts (OKCs), and to investigate the mutation status of v-raf murine sarcoma viral oncogene homolog B1 (BRAF).</p><p><strong>Methods: </strong>Following a retrospective review of the clinicopathological data of 28 OKC cases, the expressions of GLUT-1, PK-M2 and HIF-1α in these tissue samples were detected through immunohistochemistry. The BRAF mutation statuses of all cases were examined using polymerase chain reaction amplification and direct sequencing.</p><p><strong>Results: </strong>The expression levels of HIF-1α varied in 96.4% of OKC tissues, and there were higher positive rates of PKM2 (100%) and GLUT-1 (100%) in these tissues. None of the 28 OKC samples carried the BRAF mutation.</p><p><strong>Conclusion: </strong>The positive expressions of GLUT-1, PK-M2 and HIF-1α indicate that patients with OKCs undergo anaerobic glycolysis to a certain extent, but these processes appear to be irrelevant to clinicopathological features and to the BRAF mutation.</p>","PeriodicalId":74983,"journal":{"name":"The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)","volume":"25 4","pages":"285-291"},"PeriodicalIF":0.0,"publicationDate":"2022-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10403325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Features of the Sensorimotor Cortex Altered after Tooth Loss and Subsequent Implant Placement in the Maxilla of Rats.","authors":"Sheng Hao Xue,&nbsp;Jian Li,&nbsp;Jing Wen Yang,&nbsp;Zhong Ning Liu,&nbsp;Ting Jiang","doi":"10.3290/j.cjdr.b3317969","DOIUrl":"https://doi.org/10.3290/j.cjdr.b3317969","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the synergistic changes of the astrocytes and neurons in the sensorimotor cortex during the process of implant osseointegration after insertion.</p><p><strong>Methods: </strong>A total of 75 rats were allocated into three groups (n = 25): non-operated, extraction and implant. The rats in the latter two groups underwent extraction surgery of three maxillary right molars. One month later, the implant group received one titanium implant in the healed extraction socket. The rats were sacrificed on days 1, 3, 7, 14 and 28 after implantation. The brain sections, including sensory centre S1 and motor centre M1, were selected for further immunofluorescence for measurement of the synergistic morphological and quantitative changes of astrocytes and neurons.</p><p><strong>Results: </strong>In layer IV of S1, the number of astrocytes in the implant group showed a descending trend with time; on days 1, 3, 7 and 14, the number of astrocytes in both the extraction group and the implant group was significantly higher than that in the non-operated group, and there was no difference between the extraction group and the implant group; however, on day 28, the number of astrocytes in the implant group was significantly lower than that in the extraction group. In layer V of M1, on days 7, 14 and 28, the number of astrocytes in the implant group was significantly lower than that in the extraction group; on days 14 and 28, the number of astrocytes in the extraction group was significantly higher than that in the non-operated group. In layer IV of S1 or layer V of M1, the number of neurons showed no significant changes between the three groups.</p><p><strong>Conclusion: </strong>The astrocytes in the face sensorimotor cortex were activated as a reaction to oral environment changes. This kind of neuroplasticity can be reversed by oral rehabilitation with dental implants. The motor cortex may be intimately related to osseointegration and osseoperception.</p>","PeriodicalId":74983,"journal":{"name":"The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)","volume":"25 3","pages":"169-177"},"PeriodicalIF":0.0,"publicationDate":"2022-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40356804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Fracture Line Width on Radiographic Diagnosis of Vertical Root Fractures: Analysis of the Generalised Estimating Equation Model.","authors":"Kaique Leite De Lima,&nbsp;Lorena Rosa Silva,&nbsp;Mozar Andrade Mota Neto,&nbsp;Marcelo Gusmão Paraíso Cavalcanti,&nbsp;Cláudio Rodrigues Leles,&nbsp;Maria Alves Garcia Santos Silva,&nbsp;Carlos Estrela,&nbsp;Brunno Santos De Freitas Silva,&nbsp;Fernanda P Yamamoto-Silva","doi":"10.3290/j.cjdr.b3317977","DOIUrl":"https://doi.org/10.3290/j.cjdr.b3317977","url":null,"abstract":"<p><strong>Objective: </strong>To undertake a joint analysis of the influence of fracture width, dental thickness and distance of the fracture from the cortical bone on the radiographic diagnosis of vertical root fractures.</p><p><strong>Methods: </strong>Thirty-six uniradicular bovine teeth were endodontically treated and distributed into three groups according to the remaining root dentine thickness: 1.2 mm, 1.5 mm and 1.8 mm. Each group comprised 12 teeth, six with vertical root fracture and six without. Scanning electron microscopy (SEM) images of the fractured tooth groups were obtained and the fracture lines were measured. All specimens were inserted into bone defects created in bovine ribs, at different distances from the external cortical bone. Digital periapical radiographs were randomly evaluated by three blinded examiners (presence or absence of fractures).</p><p><strong>Results: </strong>The specificity for periapical radiography was found to be 89% and the accuracy rate was 57.4%. The mixed-model regression using the generalised estimating equation (GEE) model showed that the width of the fracture line and the thickness of the dental remnant play an important role in radiographic detection of vertical root fractures. There is a lower chance of correct diagnosis with fracture line widths < 0.2 mm (odds ratio [OR] 0.294, 95% confidence interval [CI] 0.103 to 0.836; P = 0.022) and tooth thicknesses < 1.2 mm (OR 0.342, 95% CI 0.157 to 0.747; P = 0.007).</p><p><strong>Conclusion: </strong>Fracture line widths < 0.2 mm and smaller root thicknesses lead to a less accurate diagnosis of vertical root fractures on periapical radiographs.</p>","PeriodicalId":74983,"journal":{"name":"The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)","volume":"25 3","pages":"197-204"},"PeriodicalIF":0.0,"publicationDate":"2022-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40356808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Social Support on Perceived Stress in Latin American and Caribbean Dental Students and Dental Practitioners during Mandatory Social Isolation within the Coronavirus Pandemic in 2020.","authors":"María Claudia Garcés-Elías,&nbsp;Roberto A León-Manco,&nbsp;Andrés A Agudelo-Suárez","doi":"10.3290/j.cjdr.b3317983","DOIUrl":"https://doi.org/10.3290/j.cjdr.b3317983","url":null,"abstract":"<p><strong>Objective: </strong>To determine the impact of social support on perceived stress in Latin American and Caribbean dental students and dental practitioners during mandatory social isolation within the coronavirus (COVID-19) pandemic in 2020.</p><p><strong>Methods: </strong>A cross-sectional study was conducted with a sample of 1812 dental students and dental practitioners from 21 Latin American and Caribbean countries. Perceived stress was assessed using the perceived stress scale (PSS-14), and the influence of social support was addressed using the Duke-UNC-11. Additionally, sociodemographic variables, knowledge of and preventive behaviour against COVID-19 and health status were considered. A descriptive, bivariate and multivariate analysis was performed through multiple linear regression.</p><p><strong>Results: </strong>In the multivariate analysis, model 4 presented R2% = 21.20 (P < 0.001), a constant of 40.049; within the model, the social support variable had a non-standardised regression coefficient (b) of -4,527 (95% CI - 5.646 to -3,408; P < 0.001), the self-perceived level of concern regarding COVID-19 was b = 1.838 (95% confidence interval [CI] 0.887 to 2.790; P < 0.001), the self-perceived health status was b = -2.191(95% CI -2.944 to -1.437; P < 0.001), the number of days in compulsory isolation was b = -0.965 (95% CI -1.908 to -0.022; P = 0.045), while the level of confinement was b = 0.923 (95%CI: 0.106-1.740; P = 0.027), age was b = -1.743 (95% CI -2.625 to -0.860; P < 0.001), sex was b = 1.324 (95% CI 0.311 to 2.337; P = 0.011) and the economic income level was b = -1.539 (95% CI -2.434 to -0.644; P = 0.001).</p><p><strong>Conclusion: </strong>An association was determined between perceived stress and social support, as well as the variables of concern about the disease, self-perceived health status, number of days and level of confinement, age, sex and economic income level, based on the experience of dental practitioners and dental students in mandatory isolation.</p>","PeriodicalId":74983,"journal":{"name":"The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)","volume":"25 3","pages":"205-213"},"PeriodicalIF":0.0,"publicationDate":"2022-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40357809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comments on \"Features of Sensorimotor Cortex Altered after Teeth Loss and Subsequent Implants Placement in the Maxilla of Rats\".","authors":"Xu Liang Deng","doi":"10.3290/j.cjdr.b3317989","DOIUrl":"https://doi.org/10.3290/j.cjdr.b3317989","url":null,"abstract":"","PeriodicalId":74983,"journal":{"name":"The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)","volume":"25 3","pages":"178"},"PeriodicalIF":0.0,"publicationDate":"2022-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40356805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}