Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology最新文献_第8页

[Influences of %T>MIC achievement probability due to the difference of the MIC measurement concentration range-analysis of meropenem for Pseudomonas aeruginosa-]. [MIC测定浓度范围差异对%T>MIC实现概率的影响-美罗培南对铜绿假单胞菌的分析]。

Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology Pub Date : 2011-01-01

Zenzo Nagasawa, Yukari Nakashima, Yumiko Fukutomi, Nozomi Uki, Koji Kusaba, Fumio Nagumo, Shoichiro Ohta, Eizaburo Sueoka, Hiroshi Miyamoto

{"title":"[Influences of %T>MIC achievement probability due to the difference of the MIC measurement concentration range-analysis of meropenem for Pseudomonas aeruginosa-].","authors":"Zenzo Nagasawa, Yukari Nakashima, Yumiko Fukutomi, Nozomi Uki, Koji Kusaba, Fumio Nagumo, Shoichiro Ohta, Eizaburo Sueoka, Hiroshi Miyamoto","doi":"","DOIUrl":"","url":null,"abstract":"We attempted to analyze any influences to %T>MIC achievement probability due to the difference of the MIC measurement concentration range of MEPM for 613 strains of Pseudomonas aeruginosa by the Monte Carlo simulation method. As for the analysis, we calculated the achievement probability of 30% and 50% for MEPM %T>MIC by the administration volume of MEPM: 250 mg, 500 mg, and 1,000 mg, the administration time: 0.5 h, and 3 h, the administration frequency: 2 times, and 3 times, and the renal excretion capability: Normal, Slight, Moderate, and High abnormal with the 3 types of MIC concentration measurement level 1) <=0.06~>=256 µg/ml: 13 levels, 2) <=0.5~>=32 µg/ml: 7 levels, and 3) <=1~>=16 µg/ml: 5 levels. As the result, we found the following findings; 1. In terms of the administration of normal renal excretion capability, 250 mg, in comparison with 500 mg and 1,000 mg, indicated the differential due to the difference of MIC measurement concentration range. 2. The administration volume of MEPM 500 mg which has been recommended shown the less differential of the achievement probability due to the difference of MIC measurement concentration range. As the renal excretion was shifted through Normal to Slight to Moderate to High abnormal, the differential of the achievement probability due to the difference of MIC measurement concentration range was gradually decreased. With these results, PK/PD analysis is possible for the 5 levels measurement concentration. It is significant that the facility using the automated microbiology analyzer can provide not only the MIC report, but also the information on the appropriate administration method for antibacterial drug by PK/PD analysis.","PeriodicalId":74740,"journal":{"name":"Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology","volume":"22 1-2","pages":"11-22"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30475490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Comparison of the quick Gram stain method to the B&M modified and favor methods]. [快速革兰氏染色法与B&M改良法及青睐法的比较]。

Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology Pub Date : 2011-01-01

Kayo Osawa, Nobumasa Kataoka, Toshio Maruo

引用次数: 0

[Rapid microbial ID system based on MALDI TOF MS-MALDI Biotyper-]. [基于MALDI TOF MS-MALDI Biotyper的微生物快速识别系统]。

Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology Pub Date : 2011-01-01

Yumiko Matsuyama

引用次数: 0

[Evolution of infectious disease diagnostic testing and the possible use of genetic testing]. [传染病诊断检测的演变和基因检测的可能应用]。

Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology Pub Date : 2011-01-01

Kazuyuki Sugahara

{"title":"[Evolution of infectious disease diagnostic testing and the possible use of genetic testing].","authors":"Kazuyuki Sugahara","doi":"","DOIUrl":"","url":null,"abstract":"A diagnostic test for infection has been developed which uses the culture method but there still remains the issue of non-culturable pathogens. Although genetic testing has emerged as a solution to this problem, it is not yet widely used. There are various reasons for this which includes the gene amplification and analysis methods used as well as the users not being familiar with the selection criteria. In recent years, by using inexpensive instrumentation it has become possible to observe specimens using fluorescent staining and to easily identify the pathogens. Also equipment for gene and protein analysis has been developed that can analyze each level of gene transcription and translation in the expressed proteins. Today, due to the many developments in both analytical methods and instrumentation, major breakthroughs are being made in clinical microbiological testing. That is, first, to classify the infecting microorganism by fluorescent staining and then to identify the microorganism using DNA sequencing and mass spectrometry. In addition, the DNA sequencing and Melting curve analysis methods are used to test for antimicrobial resistance of infectious microorganisms. For non-culturable microbes and the growth response of microbes under stress conditions, the Phenotype-Microarray method is used. Therefore, once the weaknesses of each method are understood, it is possible to provide accurate and timely information to clinicians.","PeriodicalId":74740,"journal":{"name":"Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology","volume":"22 1-2","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30475489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Abstracts of the 23rd Annual Meeting of the Association for Rapid Method and Automation in Microbiology. June 26, 2010. Gifu, Japan]. 第23届微生物快速方法与自动化学会年会摘要。2010年6月26日。日本岐阜]。

Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology Pub Date : 2010-01-01

引用次数: 0

[Comparison of the antimicrobial susceptibility testing with three automated systems for MRSA, VISA, ESBL-producing Escherichia coli and Klebsiella pneumoniae]. [MRSA、VISA、产esbl的大肠埃希菌和肺炎克雷伯菌3种自动化系统的药敏试验比较]。

Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology Pub Date : 2010-01-01

Makiko Kiyosuke, Zenzo Nagasawa, Koji Kusaba, Takayuki Masaki, Hisae Yoshimura, Hiromi To, Tomoko Mitsui, Chiasa Otsubo, Chika Narita, Tsuyoko Morooka, Hiroshi Miyamoto, Ariaki Nagayama

{"title":"[Comparison of the antimicrobial susceptibility testing with three automated systems for MRSA, VISA, ESBL-producing Escherichia coli and Klebsiella pneumoniae].","authors":"Makiko Kiyosuke, Zenzo Nagasawa, Koji Kusaba, Takayuki Masaki, Hisae Yoshimura, Hiromi To, Tomoko Mitsui, Chiasa Otsubo, Chika Narita, Tsuyoko Morooka, Hiroshi Miyamoto, Ariaki Nagayama","doi":"","DOIUrl":"","url":null,"abstract":"Some automated systems of the identification and susceptibility for microorganisms are used and prevail in hospital laboratories. One of the most serious problems is to perform accurate susceptibility testing for low-level resistant organisms, while antibiotic resistant microbes are increasing in medical fields. To evaluate automated machines for the susceptibility testing, several antibiotic resistant organisms were examined by plural technicians in some laboratories. Each strain of methicillin-resistant Staphylococcus aureus (MRSA) and vancomycinintermediate S. aureus (VISA), extended-spectrum β-lactamase (ESBL) producing Escherichia coli and Klebsiella pneumoniae was tested by three automated systems of WalkAway, VITEK2/VITEK2 compact and Phoenix for susceptibility. The results for antibiotics generated by the systems were compared to those generated by reference methods according to CLSI guidelines. The results of WalkAway, VITEK2/VITEK2 compact, and Phoenix demonstrated 92%, 91%, and 96% of reproducibilities, 92%, 94%, and 91% of MIC agreements, 0.5%, 0.8%, and 0.3% of very major error (VME) and 0.3%, 1.4%, and 2.3% of major error (ME), respectively. All automated systems had a high reproducibility even under the performance of plural technicians, although the differences of VMEs and MEs were observed among the systems. From these data, the automated systems for antimicrobial susceptibility testing were more useful for the detection of antibiotic resistant organisms by understanding the characteristics of each system.","PeriodicalId":74740,"journal":{"name":"Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology","volume":"21 1","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29510003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Epidemiology of Pandemic Influenza (H1N1) 2009 in Aichi Medical University Hospital]. 爱知医科大学附属医院2009年甲型H1N1流感流行病学研究

Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology Pub Date : 2010-01-01

Hiroya Tani, Yuka Yamagishi, Eriko Fuzimaki, Takahiko Kishi, Minehiro Goto, Hiroshige Mikamo

{"title":"[Epidemiology of Pandemic Influenza (H1N1) 2009 in Aichi Medical University Hospital].","authors":"Hiroya Tani, Yuka Yamagishi, Eriko Fuzimaki, Takahiko Kishi, Minehiro Goto, Hiroshige Mikamo","doi":"","DOIUrl":"","url":null,"abstract":"We have analyzed epidemiology of pandemic influenza (H1N1) 2009 in Aichi Medical University hospital. As a result, the characteristics of pandemic influenza (H1N1) 2009 was as follows. (1) The number of ordered rapid diagnostic test was 2.8 times compared with the seasonal influenza period. The number of ordered rapid diagnostic test of the seasonal influenza period had the peak in January to March. However, the peak in pandemic influenza (H1N1) 2009 was November. Also, the number of samples on the weekend had been more than that of the weekday. (2) Positive rate of each diagnostic kit did not have the difference between the seasonal influenza (31.3 ± 1.8%) and pandemic influenza (H1N1) 2009 (29.6%). (3) Age on most ordered samples were less than ten years old, and the number of samples in 11 to 20 years old was twice in comparison with the seasonal influenza. (4) Pandemic influenza (H1N1) 2009 in influenza A accounted for 96.9%. (5) Sensitivity and specificity of ESPLINE Influenza A&B-N (FUJIREBIO, Inc., Tokyo, Japan) to the pandemic influenza (H1N1) 2009 were 100% and 100%, respectively. Also, sensitivity and specificity of prorasuto Flu (Mitsubishi Chemical Medience Corporation, Tokyo, Japan) were 77.3%and 98.5%, respectively.","PeriodicalId":74740,"journal":{"name":"Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology","volume":"21 1","pages":"13-20"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29510004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Clinical significance on MicroScan Rapid plus series using various antibiotic-resistant bacteria]. [MicroScan Rapid plus系列使用多种耐药菌的临床意义]。

Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology Pub Date : 2010-01-01

Yumiko Fukutomi, Nozomu Uki, Megumi Oho, Mitsuhiko Sugimati, Kouji Kusaba, Zenzo Nagasawa, Yukari Nakajima, Asami Hukuoka, Yousuke Aoki, Hiroshi Miyamoto

{"title":"[Clinical significance on MicroScan Rapid plus series using various antibiotic-resistant bacteria].","authors":"Yumiko Fukutomi, Nozomu Uki, Megumi Oho, Mitsuhiko Sugimati, Kouji Kusaba, Zenzo Nagasawa, Yukari Nakajima, Asami Hukuoka, Yousuke Aoki, Hiroshi Miyamoto","doi":"","DOIUrl":"","url":null,"abstract":"MicroScan Rapid plus Neg II Series and MicroScan Rapid plus Pos Series by Siemens Healthcare Diagnostics K.K. are the panels which enable to measure identification and antimicrobial susceptibility testing quickly and we have confirmed that it is useful for detecting drug resistance bacteria. As the identification result of comparing Rapid plus series with the current panel by using 143 strains of various drug resistance bacteria, Gram positive cocci was 87. 7%, glucose fermenter was 100% and glucose non-fermenter was 77.3 in Gram negative bacilli. On the evaluation of antimicrobial susceptibility testing, Rapid plus series, in comparison with the current panel, confirmed the lower tendency of MIC value on some drugs, but it basically presented the good concordance rate. In terms of the reporting time of antimicrobial agent, non-fermenter or MRCNS reported the result as needed after 8 hours and it took a little longer time for the report of antimicrobial agent. On the other hand, 80% or higher of antimicrobial agent on panel was reported for intestinal bacteria in 4.5 hours and for MRSA in 6.5 hours. It enabled to report the testing result on the same day. Due to the results above, Rapid plus series was highly valued on the usability, such as the early detection of drug resistance bacteria and the selection of therapeutic agents.","PeriodicalId":74740,"journal":{"name":"Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology","volume":"21 2","pages":"95-105"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30265280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Adenovirus infection and rapid adenovirus antigen detection kits]. [腺病毒感染及快速腺病毒抗原检测试剂盒]。

Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology Pub Date : 2010-01-01

Kanji Sakamoto

引用次数: 0

[Evaluation of measurement method of a bacterial sepsis marker, procalcitonin]. 细菌性败血症标志物降钙素原测定方法的评价

Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology Pub Date : 2010-01-01

Shoko Adachi, Koichi Nakao

引用次数: 0