Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology最新文献_第10页

[Evaluation of rapid antimicrobial susceptibility test for Staphylococcus aureus by chemiluminescent assay and its application for screening of beta-lactam antibiotic induced vancomycin-resistant MRSA]. [化学发光法快速金黄色葡萄球菌药敏试验评价及其在β -内酰胺类抗生素诱导万古霉素耐药MRSA筛选中的应用]。

Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology Pub Date : 2008-01-01

Zenzo Nagasawa, Yukari Nakashima, Megumi Oho, Kouji Kusaba, Isao Manome, Ariaki Nagayama

{"title":"[Evaluation of rapid antimicrobial susceptibility test for Staphylococcus aureus by chemiluminescent assay and its application for screening of beta-lactam antibiotic induced vancomycin-resistant MRSA].","authors":"Zenzo Nagasawa, Yukari Nakashima, Megumi Oho, Kouji Kusaba, Isao Manome, Ariaki Nagayama","doi":"","DOIUrl":"","url":null,"abstract":"Minimum inhibitory concentrations (MICs) of vancomycin (VCM) and teicoplanin (TEIC) were measured using a novel susceptibility test based on the chemiluminescence assay method (CA) (Rapid-Lumi Eiken; Eiken Chemicals, Tokyo, Japan) against 84 strains of Staphylococcus aureus, consisting of 82 strains of methicillin-resistant S. aureus (MRSA) from clinical isolated, S. aureus Mu3 involving beta-lactam antibiotic induced vancomycin (VCM) resistant MRSA (BIVR) and methicillin-susceptible S. aureus ATCC 29213. The results were in good accordance with the values determined by Clinical and Laboratory Standards Institute (CLSI): i.e., 100% (84/84) of consistency for VCM and 95% (80/84) for TEIC, respectively. In addition, BIVR strains were properly estimated from the results of the CA method and using the BIVR detection method with Mu3 agar (Mu3 Agar method), even though the incubation times was very short (2-4 h). In conclusion, it was found that the new method is reliable and rapid to detect BIVR strains in clinical laboratories.","PeriodicalId":74740,"journal":{"name":"Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology","volume":"19 1-2","pages":"7-15"},"PeriodicalIF":0.0,"publicationDate":"2008-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28291329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Epidemiological search for vancomycin-resistant enterococci in mainland of the Ryukyus]. [琉球大陆地区万古霉素耐药肠球菌的流行病学研究]。

Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology Pub Date : 2008-01-01

Kyoko Kisanuki, Miyako Higa, Isamu Nakasone, Chika M Shiohira, Nobuhisa Yamane

引用次数: 0

[The present condition of rapid diagnostic reagent for microbiology]. 微生物学快速诊断试剂的现状

Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology Pub Date : 2007-01-01

Mitsuharu Murase

引用次数: 0

[Cloning of Clostridium perfringens alpha-toxin gene and extracellular expression in Escherichia coli]. 产气荚膜梭菌α毒素基因的克隆及在大肠杆菌中的胞外表达

Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology Pub Date : 2007-01-01

Masaharu Inoue, Maho Kikuchi, Tomoe Komoriya, Kunitomo Watanabe, Hideki Kouno

{"title":"[Cloning of Clostridium perfringens alpha-toxin gene and extracellular expression in Escherichia coli].","authors":"Masaharu Inoue, Maho Kikuchi, Tomoe Komoriya, Kunitomo Watanabe, Hideki Kouno","doi":"","DOIUrl":"","url":null,"abstract":"Clostridium perfringens (C. perfringens) is a Gram-positive bacterial pathogen that widely propagets in the soil and the gastrointestinal tract of human and animals. This bacteria causes food poisoning, gas gangrene and other various range of infectious diseases. But there is no standard diagnosis method of C. perfringens. In order to develop a new type of immunoassay for clinical purpose, we studied expression and extracellular secretion of recombinant alpha-toxin having enzyme activity in E. coli expression system. Cloning was carried out after PCR amplification from C. perfringens GAI 94074 which was clinical isolate. Three kinds of fragment were cloned using pET100/D-TOPO vector. These fragments coded for ribosome binding site, signal peptide, and alpha-toxin gene respectively. Recombinant pET100 plasmid transformed into TOP 10 cells and the obtained plasmids were transformed into BL21 (DE3) cells. Then, the transformants were induced expression with IPTG. In conclusion, we successfully cloned, expressed and exteracellular secreted C. perfringens alpha-toxin containing signal peptide. Biologically, the obtained recombinant protein was positive for phospholipase C activity.","PeriodicalId":74740,"journal":{"name":"Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology","volume":"18 2","pages":"127-35"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27188549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of latex turbidimetric immunoassay for rapid and sensitive detection of influenza virus. 快速灵敏检测流感病毒的乳胶比浊免疫分析法的建立。

Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology Pub Date : 2007-01-01

Hiroshi Nemoto, Tomoe Komoriya, Hideki Kohno

{"title":"Development of latex turbidimetric immunoassay for rapid and sensitive detection of influenza virus.","authors":"Hiroshi Nemoto, Tomoe Komoriya, Hideki Kohno","doi":"","DOIUrl":"","url":null,"abstract":"For the rapid and sensitive detection of influenza A and B viruses, a latex turbidimetric immunoassay (LTIA) was developed using latex reagents prepared by the sensitization of anti-influenza A or B monoclonal antibodies on latex particles. We measured the immunoreactivity of these latex reagents to influenza A and B viral antigens. The sensitivity and specificity of LTIA and reverse transcription-polymerase chain reaction (RT-PCR) for the detection of these viruses in clinical specimens (96 nasal swabs) were compared. The absorbance change in the latex agglutination reaction increased for each latex reagent with increasing concentration of the viral antigens. Reaction curves were obtained with each concentration of viral antigens for 5 min. The effective concentration ranges were 0-10 microg/ml for influenza A and 0-20 microg/ml for influenza B. The LTIA using clinical specimens revealed 8 positive and 73 negative results for influenza A and 15 positive and 52 negative results for influenza B. The sensitivities and specificities were 89% (8/9) and 84% (73/87), respectively, for influenza A and 100% (15/15) and 64% (52/81), respectively, for influenza B. The corresponding positive predictive values (PPV) were 36% (8/22) for influenza A and 34% (15/44) for influenza B. The negative predictive values (NPV) were approximately 99% (73/74) for influenza A and 100% (52/52) for influenza B. The LTIA is a rapid and sensitive method for detection of the influenza virus; It can be used for high throughput assay by automatic measurement and can potentially be used during influenza pandemics.","PeriodicalId":74740,"journal":{"name":"Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology","volume":"18 2","pages":"117-26"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27188548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Laboratory-based evaluation of TOX A/B QUIK CHEK "NISSUI" to detect toxins A and B of clostridium difficile]. TOX A/B QUIK CHEK“NISSUI”检测艰难梭菌毒素A和毒素B的实验室评价

Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology Pub Date : 2007-01-01

Isamu Nakasone, Chika M Shiohira, Nobuhisa Yamane

{"title":"[Laboratory-based evaluation of TOX A/B QUIK CHEK \"NISSUI\" to detect toxins A and B of clostridium difficile].","authors":"Isamu Nakasone, Chika M Shiohira, Nobuhisa Yamane","doi":"","DOIUrl":"","url":null,"abstract":"The TOX A/B QUIK CHEK \"NISSUI\" which detects both toxin A (TcdA) and toxin B (TcdB) of Clostridium difficile in stool specimens through immunochromatography was first approved to be released in Japan, and we evaluated its accuracy. In the evaluation, the TOX A/B QUIK CHEK \"NISSUI\" could correctly detect TcdA and TcdB in solution and in stool specimens spiked with culture broth of TcdA and/or TcdB-producing isolates of C. difficile. The minimum detectable concentrations for TcdA and TcdB were determined to be < or =0.32 ng/ml and < or =0.63 ng/ml, respectively. The TOX A/B QUIK CHEK \"NISSUI\" gave the consistent results with the colon-endoscopic diagnosis, that is, all the 10 stool specimens from the patients with pseudomembranous colitis were read as being positive, but negative for five patients without any C. difficile-associated disease (CDAD). Of 10 positive stool specimens, one was read as being negative by the commercially available test reagents that can detect only TcdA. In clinical evaluation, a total of 240 stool specimens were tested. Of these, the TOX A/B QUIK CHEK \"NISSUI\" gave 19 positive results, and TcdA and/or TcdB-producing strains of C. difficile were successfully isolated from all the positive stool specimens, except one. Whereas, of 221 negative stool specimens, 28 isolates of C. difficile were recovered and 11 isolates were identified as TcdA and/or TcdB-producing strains. With these results, it can be concluded that the TOX A/B QUIK CHEK \"NISSUI\" can correctly detect both TcdA and TcdB of C. difficile, and should be promptly applied to clinical microbiology laboratory to make a definite diagnosis of CDAD, particularly for the CDAD caused by the TcdA-negative but TcdB-positive mutant strains.","PeriodicalId":74740,"journal":{"name":"Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology","volume":"18 2","pages":"109-16"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27188547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Evaluation for anaerobic culture system: Anoxomat Mart II]. 厌氧培养系统的评价:Anoxomat Mart II。

Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology Pub Date : 2007-01-01

Yuji Kikuchi, Hiromi Sasaki, Yukie Furuhata, Yoko Tazawa, Hajime Horiuchi, Jun Okada

{"title":"[Evaluation for anaerobic culture system: Anoxomat Mart II].","authors":"Yuji Kikuchi, Hiromi Sasaki, Yukie Furuhata, Yoko Tazawa, Hajime Horiuchi, Jun Okada","doi":"","DOIUrl":"","url":null,"abstract":"Anoxomat Mart II (Mart Microbiology BV, Lichtenvooorde, Netherlands, Central Scientific Commerce Inc., Tokyo, Japan) is an anaerobic jar apparatus which uses a vacuum pump in combination with catalyst as gas replacement procedure to remove all traces of oxygen. As we had a chance to use Anoxomat Mart II, we compared it with other two anaerobic culture methods; namely AnaeroPack anaero (Mitsubishi Gas Chemical Co., Tokyo, Japan) which employs anaerobic jar method, and Concept400 (RUSKINN TECHNOLOGY LTD, England; Central Scientific Commerce INc., Tokyo, Japan) which uses anaerobic chamber method. We used 10 different species of anaerobic bacteria obtained from ATCC. One strain each of 10 species was cultured and examined for measurement of the sensitibity of an anaerobic indicator, th number of bacteria after 48 hour culture, the diameter of colonies, and MIC value. As a result, the time to reach the anaerobic condition was around 30 minutes by the Mart II against around 60 minutes by the AnaeroPack anaero. There was no difference concerning the number of bacteria after 48 hour culture among three methods. But anaerobic bacteria cultured by Mart II tended to make bigger colonies compared to other two methods in the 5 strains out of 9, except for one strain in which the diameter of colonies could not be measured. On the other hand, the comparison of MIC value showed good correlation in 11 antibiotics out of 12 among three methods. The MIC value of 11 antibiotics fitted within 1-fold difference, and 2-fold difference was observed in only one antibiotic. Mart II is so small that it does cheep consumables. From these reasons, we concluded that Mart II can be one of the useful anerobic culture methods.","PeriodicalId":74740,"journal":{"name":"Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology","volume":"18 1","pages":"11-7"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26984473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Evaluation of beta-D-glucan density in blood after drinking an extraction element of Agaricus blazei murill]. [饮用姬松茸提取物后血液中β - d -葡聚糖密度的评价]。

Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology Pub Date : 2007-01-01

Hiroshi Shibata, Hideyuki Furuse, Yuki Taniguchi, Mutsuko Itoh, Atsushi Nagai, Junichi Masuda, Yoshiroh Iijima

{"title":"[Evaluation of beta-D-glucan density in blood after drinking an extraction element of Agaricus blazei murill].","authors":"Hiroshi Shibata, Hideyuki Furuse, Yuki Taniguchi, Mutsuko Itoh, Atsushi Nagai, Junichi Masuda, Yoshiroh Iijima","doi":"","DOIUrl":"","url":null,"abstract":"beta-D-Glucan (beta-G) measurement in blood is useful for prompt diagnosis, selection of treatment and therapeutic evaluation for deep fungal infection. A patient with high beta-G blood was not improved by strong anti-fungal treatment in our hospital, and the beta-G levels in the blood fell immediately after discontinuing ingestion of the agaricus mushroom extracted element (sennseiro). To verify the elevation of beta-G concentration in the blood after intake of an agaricus mushroom extraction element, beta-G concentration in the blood was measured 4 days after 9 volunteers drank 2 g of agaricus mushroom extraction element, \"SSG plus 35\" twice a day for 3 days. A low value of beta-G in blood was detected in one person (11.1%), which demonstrated that beta-G concentration in the blood increased by oral ingestion of the agaricus element although the reason was unclear why it was detected in only one person. Taken together, when high beta-D-glucan is identified by uncertain cause or in spite of enough anti-fungal medication, it is necessary to confirm whether a patient has ingested health food containing an agaricus mushroom element or other fungus elements to avoid needless treatment with anti-fungal medicine.","PeriodicalId":74740,"journal":{"name":"Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology","volume":"18 2","pages":"103-7"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27188051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[How to analyze the diversity of genetic alteration]. 【如何分析遗传变异的多样性】。

Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology Pub Date : 2006-01-01

Toshio Nikaido

引用次数: 0

Fundamental study of a newly developed medium on detection of Lactobacillus. 新型乳酸菌检测培养基的基础研究。

Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology Pub Date : 2006-01-01

Yoshihiro Tanaka, Masayuki Naganawa, Masatoshi Sakai, Shigeru Saito

{"title":"Fundamental study of a newly developed medium on detection of Lactobacillus.","authors":"Yoshihiro Tanaka, Masayuki Naganawa, Masatoshi Sakai, Shigeru Saito","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To investigate the specificity of a newly developed Lactobacillus medium (LB medium) and its usefulness for the detection of Lactobacillus.Method: Chlorophenol red was added to Rogosa SL Broth to make the LB medium whose pH was adjusted to 5.90. After incubation of Lactobacillus in LB medium for 24 hours, the correlation between the number of bacteria and the medium pH was examined. The change of pH was also examined when other microbes coexisted with Lactobacillus.Results: Six species of Lactobacillus were tested. The medium pH decreased to 4 approximately 5 after incubation of any of these species for 24 hours. Though there were some differences in the acid-producing ability among species, a significant negative correlation (r = -0.93 approximately -0.99) was found between the initial number of bacteria and the medium pH with all species tested. The color of the medium did not change after incubation of other bacteria that might also be found in the normal vagina. The performance of the medium was not affected by the presence of large numbers of other microbes. In L. gasseri JCM 1131, the test microbe for color sample, the number of microbes whose color changed (red-->orange-->yellow) were approximately consistent with that of microbes reported in bacterial vaginosis (BV).Conclusion: This method can be easily applied to detect Lactobacillus species as the number of Lactobacillus can be estimated based on the change in color of the medium for less than 24 hours after incubation.","PeriodicalId":74740,"journal":{"name":"Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology","volume":"17 1","pages":"23-32"},"PeriodicalIF":0.0,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27005738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0