Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology最新文献

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[Investigation of the procalcitonin and microbiology test and antibiotics situation from our hospital]. [我院降钙素原、微生物学检测及抗生素使用情况调查]。
Kotaro Fujii, Kuniko Yamada, Junko Sano, Dai Mashima, Hiroko Takeda
{"title":"[Investigation of the procalcitonin and microbiology test and antibiotics situation from our hospital].","authors":"Kotaro Fujii,&nbsp;Kuniko Yamada,&nbsp;Junko Sano,&nbsp;Dai Mashima,&nbsp;Hiroko Takeda","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Procalcitonin (PCT) was first described as a sepsis-associated protein in 1993. PCT is increased in the blood at the time of infection by bacteria. Therefore, it is used as an auxiliary indicator of sepsis diagnosis. In addition, PCT is reduced quickly by antibiotics. And use as a stop or change marker is also expected. We have investigated the antimicrobial use and microbial testing of measurement patient and PCT running performance. Number of requests was 3,387 cases (inpatient 2,649 and outpatient 742 cases) for one year. It was subject to the 820 cases that had inspection request to July to October 2012. In 820 cases, 57 cases had exhibited a PCT >0.5 ng/ml and diagnosed with infectious diseases. In 57 cases, 44 cases (77%) were performed microbiology and blood culture. And only blood culture performed in 8 (14%), blood culture and microbiology is not performed for 5 cases (9%), In 21 (40%) cases of 52 cases performed the blood culture shown positive. Detecting bacteria accounted for more than half in 17 cases of Gram-negative bacilli. Also, it had exhibited a systemic inflammatory response syndrome (SIRS) in 18 cases. Antibiotics have been used in all cases regardless of implementation of the microbiology test. If sepsis is suspected, it is necessary for diagnosis is done correctly and quickly. Therefore, PCT has been suggested high usefulness by examining in the hospital. It is required that the reference identification and drug susceptibility results of the pathogenic bacterium combination of microbiology test and the PCT. We considered useful to PCT monitoring that as an indicator of antimicrobial agents change or shorten of antibiotic use period. Future, proactive use of clinical practice is expected. </p>","PeriodicalId":74740,"journal":{"name":"Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology","volume":"25 1","pages":"7-15"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32932464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Clinical utility of Pourmedia GBS agar on screening for vaginal colonization of Group B Streptococcus]. [Pourmedia GBS琼脂筛选阴道B群链球菌定植的临床应用]。
Mitsunori Kaneda, Hiromi Nagasaki, Megumi Tasaki, Kiyoshi Kamiyama
{"title":"[Clinical utility of Pourmedia GBS agar on screening for vaginal colonization of Group B Streptococcus].","authors":"Mitsunori Kaneda,&nbsp;Hiromi Nagasaki,&nbsp;Megumi Tasaki,&nbsp;Kiyoshi Kamiyama","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Group B Streptococcus (GBS) are normal flora of the vagina and intestinal, but if the pregnant woman was infected with GBS in the vagina, miscarriage or premature would occur or the newborn would be developed to severe GBS infection. It is recommended that the inspection of GBS on all pregnant women by Japan Society of Obstetrics and Gynecology (JSOG) and Center for Disease Control and Prevention (CDC). We examined the comparison of detection rate between Pourmedia GBS agar (Eiken Chemical Co., Ltd.) and Nissui Separated Plate Sheep Blood Agar/BTB Lactose Agar medium (Nissui Pharmaceutical Co., Ltd.) on 112 sample. The positive rate of Pourmedia GBS agar was 21.4% (24/112 samples), Whereas Nissui Separated Plate Sheep Blood Agar/BTB Lactose Agar medium was 17.8% (20/112 samples). It was found that the detection rate was improved by using Pourmedia GBS agar on GBS screening test of vaginal swab. </p>","PeriodicalId":74740,"journal":{"name":"Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology","volume":"25 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32932462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Evaluation of the sensitivity of a densitometry system, in judging the result of influenza virus antigen-detection kit using immunochromatography]. 【用免疫层析法判断流感病毒抗原检测试剂盒结果时,密度测定系统的灵敏度评价】。
Ikuo Yamaguchi, Tomoe Aoyama, Masaru Yamamoto, Keiko Kinosita, Yumi Ito
{"title":"[Evaluation of the sensitivity of a densitometry system, in judging the result of influenza virus antigen-detection kit using immunochromatography].","authors":"Ikuo Yamaguchi,&nbsp;Tomoe Aoyama,&nbsp;Masaru Yamamoto,&nbsp;Keiko Kinosita,&nbsp;Yumi Ito","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Immunochromatography viral antigen-detection kits have become popular in clinical settings in Japan. Influenza virus detection kit is one of them. It is sometimes used in early phase of the disease, combined with the early treatment with anti-influenza drugs. Most of them are invented to visually read the test line on their kits. However, we should be careful about their reliability of them. Sometimes human errors occur at the visual tests, and they have different sensitivities among the kits from different companies. In this report, we evaluated the sensitivity of BD Veritor System Flu with its reader by comparing with conventional visual tests. A total of 84 people including laboratory technologists were asked to visually read test line and their answers were compared with results of BD Veritor System Reader. This study showed that the lower the concentration of standard sample was applied, the greater the error ratio of visual test became, indicating the stable sensitivity of Veritor System. Moreover, the sensitivity was compared with three other major products approved in Japan, using four influenza viruses: type A of H1N1 seasonal 2009, H1N1 pandemic 2009, H3N2 seasonal 2012 and type B of seasonal 2012. It was indicated that Veritor System had the highest limit of detection from the kits.</p>","PeriodicalId":74740,"journal":{"name":"Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology","volume":"24 2","pages":"69-70"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32544125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Rapid diagnosis and viral infections topics, interpretation of the results and characteristics of the virus-detection]. 【快速诊断与病毒感染专题,解读病毒检测的结果和特点】。
Keiji Iida
{"title":"[Rapid diagnosis and viral infections topics, interpretation of the results and characteristics of the virus-detection].","authors":"Keiji Iida","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":74740,"journal":{"name":"Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology","volume":"24 2","pages":"63-8"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32544124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Abstracts of the 26th Annual Meeting of the Association for Rapid Method and Automation in Microbiology. July 26, 2014. Okayama, Japan]. 第26届微生物快速方法与自动化学会年会摘要。2014年7月26日。日本冈山,日本]。
{"title":"[Abstracts of the 26th Annual Meeting of the Association for Rapid Method and Automation in Microbiology. July 26, 2014. Okayama, Japan].","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":74740,"journal":{"name":"Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology","volume":"Suppl Sokai ","pages":"15-41"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33946802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[A retrospective study of the relationship between bacterial numbers from central venous catheter tip cultures and blood cultures for evaluating central line-associated bloodstream infections]. [中心静脉导管尖端培养细菌数量与血液培养评估中心静脉相关血流感染之间关系的回顾性研究]。
Hirofumi Ohtaki, Kiyofumi Ohkusu, Asami Nakayama, Jun Yonetamari, Kohei Ando, Takashi Miyazaki, Hirotoshi Ohta, Nobuyuki Furuta, Tamayo Watanabe, Hiroyasu Ito, Nobuo Murakami, Mitsuru Seishima
{"title":"[A retrospective study of the relationship between bacterial numbers from central venous catheter tip cultures and blood cultures for evaluating central line-associated bloodstream infections].","authors":"Hirofumi Ohtaki,&nbsp;Kiyofumi Ohkusu,&nbsp;Asami Nakayama,&nbsp;Jun Yonetamari,&nbsp;Kohei Ando,&nbsp;Takashi Miyazaki,&nbsp;Hirotoshi Ohta,&nbsp;Nobuyuki Furuta,&nbsp;Tamayo Watanabe,&nbsp;Hiroyasu Ito,&nbsp;Nobuo Murakami,&nbsp;Mitsuru Seishima","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Catheter-related bloodstream infection (CRBSI) is an infectious disease requiring special attention. It is a common cause of nosocomial infections; catheter insertion into the central veins particularly increases the risk of infection (CLA-BSI: central line-associated bloodstream infection). We examined the relationship between the number of bacterial colonies cultured from shredded central venous catheter (CVC) tips and from blood cultures in our hospital from 2011 to 2012. Coagulase-negative staphylococci topped the list of microbe isolated from the CVC tip culture, followed by Pseudomonas aeruginosa, Staphylococcus aureus, and Candida spp. S. aureus and Candida spp., with growth of over 15 colony-forming units in the CVC tip culture, were also detected at high rates in the blood culture. However, gramnegative bacilli (Enterobacteriaceae and P. aeruginosa) did not show a similar increase in colony number in the CVC tip culture. Because microbes adhering to shredded catheter tips are readily detected by culture, this method is useful as a routine diagnostic test. In addition, prompt clinical reporting of the bacterial number of serious CLA-BSI-causing S. aureus and Candida spp. isolated from CVC tips could contribute to earlier CLA-BSI diagnosis. </p>","PeriodicalId":74740,"journal":{"name":"Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology","volume":"24 2","pages":"39-43"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32230703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Contribution of rapid diagnostic test in the treatment of infectious diseases]. [快速诊断试验在传染病治疗中的贡献]。
Ai Suzaki
{"title":"[Contribution of rapid diagnostic test in the treatment of infectious diseases].","authors":"Ai Suzaki","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>According to aging of population and medical progress, the risk and mortality rate of pneumonia have been increased. In the causative organism inspection, smear-culture examination of sputum is the gold standard. When it is difficult to obtain high quality sputum and to culture the causative organism, or when antibiotics are previously administered, we have no choice but to start the treatment with pathogenic bacteria unknown. In these cases, it is necessary to select an appropriate test method for expecting pathogen. If bacteria are detected in laboratory culture, it is difficult to distinguish whether it is a causative or colonizers. Future development is expected in rapid diagnostic methods.</p>","PeriodicalId":74740,"journal":{"name":"Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology","volume":"24 2","pages":"57-61"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32544123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Development of norovirus rapid detection kit "QuickNavi™-Norovirus2"]. 诺如病毒快速检测试剂盒“QuickNavi™-Norovirus2”的研制
Yuji Saito, Daisuke Kato, Koichi Inano
{"title":"[Development of norovirus rapid detection kit \"QuickNavi™-Norovirus2\"].","authors":"Yuji Saito,&nbsp;Daisuke Kato,&nbsp;Koichi Inano","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We have evaluated our new Norovirus rapid detection kit \"QuickNavi™-Norovirus2\" (Denka Seiken Co., Ltd.). The kit is based on Immunochromatographic method using colored latex particles. In addition to simple preparations giving quick (15 min) results, our newly developed kit enables specimen collection directly from rectum and can also be used with neonatal stool samples, both of which cannot be used with the current version of the kit, \"QuickNavi™-Norovirus.\" Clinical study (stool samples, n=172) shows sensitivity, specificity and accuracy of the test compared to RT-PCR are 92.0%, 98.3%, and 94.2%, respectively. The result also showed good correlation with QuickNavi™-Norovirus. In this study, the new Norovirus rapid detection kit has shown sufficient sensitivity and specificity. Their performance, simple preparation enabling rectal swab samples, and quick results can be useful for day to day clinical practice when diagnosing Norovirus infection.</p>","PeriodicalId":74740,"journal":{"name":"Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology","volume":"24 2","pages":"45-50"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32229483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Differentiation of influenza (Flu) type A, type B, and respiratory syncytial virus (RSV) by QuickNavi™-Flu+RSV]. [用 QuickNavi™-Flu+RSV 鉴别甲型流感、乙型流感和呼吸道合胞病毒 (RSV)]。
Risa Kohiyama, Takashi Miyazawa, Nobuko Shibano, Koichi Inano
{"title":"[Differentiation of influenza (Flu) type A, type B, and respiratory syncytial virus (RSV) by QuickNavi™-Flu+RSV].","authors":"Risa Kohiyama, Takashi Miyazawa, Nobuko Shibano, Koichi Inano","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Because it is not easy to differentiate Influenza virus (Flu) from RS virus (RSV) just by clinical symptoms, to accurately diagnose those viruses in conjunction with patient's clinical symptoms, rapid diagnostic kits has been used separately for each of those viruses. In our new study, we have developed a new rapid diagnostic kit, QuickNavi™-Flu+RSV. The kit can detect Flu A, Flu B, and RSV antigens with a single sample collection and an assay. Total of 2,873 cases (including nasopharyngeal swabs and nasopharyngeal aspirates specimens) in 2010/2011 and 2011/2012 seasons were evaluated with QuickNavi™-Flu+RSV and a commercially available kit. Sensitivity, specificity, and accuracy of Flu type A, type B, and RSV were above 95% when compared to commercially available kits (QuickNavi™-Flu and QuickNavi™-RSV) and considered to be equivalent to the commercially available kits. In 2011/2012 season, RSV infections increased prior to Flu season and continued during the peak of the Flu season. The kit can contribute to accurate diagnosis of Flu and RSV infections since co-infection cases have also been reported during the 2011/2012 season. QuickNavi™-Flu+RSV is useful for differential diagnosis of respiratory infectious diseases since it can detect Flu type A, type B, and RSV virus antigens with a single sample collection. </p>","PeriodicalId":74740,"journal":{"name":"Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology","volume":"24 2","pages":"51-6"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32229484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[An immunochromatographic device for rapid detection of ribosomal protein L7/L12 as a diagnostic test for Mycoplasma pneumonia]. [一种快速检测核糖体蛋白L7/L12的免疫层析装置用于支原体肺炎的诊断试验]。
Hiroshi Itoh
{"title":"[An immunochromatographic device for rapid detection of ribosomal protein L7/L12 as a diagnostic test for Mycoplasma pneumonia].","authors":"Hiroshi Itoh","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":74740,"journal":{"name":"Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology","volume":"25 1","pages":"17"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34059157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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