Molecular biology international最新文献_第8页

Relative Copy Number Variations of CYP2C19 in South Indian Population. 南印度人群CYP2C19基因的相对拷贝数变异

Molecular biology international Pub Date : 2012-01-01 Epub Date: 2012-06-25 DOI: 10.1155/2012/643856

Anichavezhi Devendran, Chakradhara Rao Satyanarayana Uppugunduri, Rajan Sundaram, Deepak Gopal Shewade, Krishnamoorthy Rajagopal, Adithan Chandrasekaran

引用次数: 3

Virtual interactomics of proteins from biochemical standpoint. 从生化角度看蛋白质的虚拟相互作用组学。

Molecular biology international Pub Date : 2012-01-01 Epub Date: 2012-08-08 DOI: 10.1155/2012/976385

Jaroslav Kubrycht, Karel Sigler, Pavel Souček

{"title":"Virtual interactomics of proteins from biochemical standpoint.","authors":"Jaroslav Kubrycht, Karel Sigler, Pavel Souček","doi":"10.1155/2012/976385","DOIUrl":"10.1155/2012/976385","url":null,"abstract":"Virtual interactomics represents a rapidly developing scientific area on the boundary line of bioinformatics and interactomics. Protein-related virtual interactomics then comprises instrumental tools for prediction, simulation, and networking of the majority of interactions important for structural and individual reproduction, differentiation, recognition, signaling, regulation, and metabolic pathways of cells and organisms. Here, we describe the main areas of virtual protein interactomics, that is, structurally based comparative analysis and prediction of functionally important interacting sites, mimotope-assisted and combined epitope prediction, molecular (protein) docking studies, and investigation of protein interaction networks. Detailed information about some interesting methodological approaches and online accessible programs or databases is displayed in our tables. Considerable part of the text deals with the searches for common conserved or functionally convergent protein regions and subgraphs of conserved interaction networks, new outstanding trends and clinically interesting results. In agreement with the presented data and relationships, virtual interactomic tools improve our scientific knowledge, help us to formulate working hypotheses, and they frequently also mediate variously important in silico simulations.","PeriodicalId":74217,"journal":{"name":"Molecular biology international","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/976385","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30863728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Probing Retroviral and Retrotransposon Genome Structures: The "SHAPE" of Things to Come. 探测逆转录病毒和反转录转座子基因组结构:未来事物的“形状”。

Molecular biology international Pub Date : 2012-01-01 Epub Date: 2012-05-17 DOI: 10.1155/2012/530754

Joanna Sztuba-Solinska, Stuart F J Le Grice

{"title":"Probing Retroviral and Retrotransposon Genome Structures: The \"SHAPE\" of Things to Come.","authors":"Joanna Sztuba-Solinska, Stuart F J Le Grice","doi":"10.1155/2012/530754","DOIUrl":"https://doi.org/10.1155/2012/530754","url":null,"abstract":"Understanding the nuances of RNA structure as they pertain to biological function remains a formidable challenge for retrovirus research and development of RNA-based therapeutics, an area of particular importance with respect to combating HIV infection. Although a variety of chemical and enzymatic RNA probing techniques have been successfully employed for more than 30 years, they primarily interrogate small (100–500 nt) RNAs that have been removed from their biological context, potentially eliminating long-range tertiary interactions (such as kissing loops and pseudoknots) that may play a critical regulatory role. Selective 2′ hydroxyl acylation analyzed by primer extension (SHAPE), pioneered recently by Merino and colleagues, represents a facile, user-friendly technology capable of interrogating RNA structure with a single reagent and, combined with automated capillary electrophoresis, can analyze an entire 10,000-nucleotide RNA genome in a matter of weeks. Despite these obvious advantages, SHAPE essentially provides a nucleotide “connectivity map,” conversion of which into a 3-D structure requires a variety of complementary approaches. This paper summarizes contributions from SHAPE towards our understanding of the structure of retroviral genomes, modifications to which technology that have been developed to address some of its limitations, and future challenges.","PeriodicalId":74217,"journal":{"name":"Molecular biology international","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/530754","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30680110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Relationship between DNA Mismatch Repair Deficiency and Endometrial Cancer. DNA错配修复缺陷与子宫内膜癌的关系。

Molecular biology international Pub Date : 2011-01-01 Epub Date: 2011-12-08 DOI: 10.4061/2011/256063

Kenta Masuda, Kouji Banno, Megumi Yanokura, Yusuke Kobayashi, Iori Kisu, Arisa Ueki, Asuka Ono, Nana Asahara, Hiroyuki Nomura, Akira Hirasawa, Nobuyuki Susumu, Daisuke Aoki

{"title":"Relationship between DNA Mismatch Repair Deficiency and Endometrial Cancer.","authors":"Kenta Masuda, Kouji Banno, Megumi Yanokura, Yusuke Kobayashi, Iori Kisu, Arisa Ueki, Asuka Ono, Nana Asahara, Hiroyuki Nomura, Akira Hirasawa, Nobuyuki Susumu, Daisuke Aoki","doi":"10.4061/2011/256063","DOIUrl":"https://doi.org/10.4061/2011/256063","url":null,"abstract":"Some cases of endometrial cancer are associated with a familial tumor and are referred to as hereditary nonpolyposis colorectal cancer (HNPCC or Lynch syndrome). Lynch syndrome is thought to be induced by germline mutation of the DNA mismatch repair (MMR) gene. An aberration in the MMR gene prevents accurate repair of base mismatches produced during DNA replication. This phenomenon can lead to an increased frequency of errors in target genes involved in carcinogenesis, resulting in cancerization of the cell. On the other hand, aberrant DNA methylation is thought to play a key role in sporadic endometrial carcinogenesis. Hypermethylation of unmethylated CpG islands in the promoter regions of cancer-related genes associated with DNA repair leads to the cell becoming cancerous. Thus, both genetic and epigenetic changes are intricately involved in the process through which cells become cancerous. In this review, we introduce the latest findings on the DNA mismatch repair pathway in endometrial cancer.","PeriodicalId":74217,"journal":{"name":"Molecular biology international","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3235885/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30330760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22

Databases and bioinformatics tools for the study of DNA repair. DNA修复研究的数据库和生物信息学工具。

Molecular biology international Pub Date : 2011-01-01 Epub Date: 2011-07-14 DOI: 10.4061/2011/475718

Kaja Milanowska, Kristian Rother, Janusz M Bujnicki

引用次数: 11

Target Identification and Intervention Strategies against Kinetoplastid Protozoan Parasites. 着丝质体原生动物寄生虫的靶点鉴定及干预策略。

Molecular biology international Pub Date : 2011-01-01 Epub Date: 2011-08-09 DOI: 10.4061/2011/185413

Hemanta K Majumder, Wanderley de Souza, Kwang Poo Chang

{"title":"Target Identification and Intervention Strategies against Kinetoplastid Protozoan Parasites.","authors":"Hemanta K Majumder, Wanderley de Souza, Kwang Poo Chang","doi":"10.4061/2011/185413","DOIUrl":"https://doi.org/10.4061/2011/185413","url":null,"abstract":"The past few decades have been marked by numerous admirable research efforts and promising technological advancements in the field of research on protozoan parasites. The parasites of this genre cause some devastating diseases that pose alarming threat to the mankind. Though several intervention strategies have been developed to get rid of these parasites, they always seem to frustrate the efforts of the scientific community sooner or later. The intervention strategies include identification of novel drug targets, development of target-based therapy, and development of vaccines. that provide significant impetus in the field of research pertaining to these parasites. In this context, several reviews have appeared in the past few years elucidating different drug targets in these parasites. For example, Das et al. [1], Balana-Fouce et al. [2], and others have described the role of topoisomerases as potential drug targets in these kinetoplastid protozoa. Urbina [3] has described the lipid biosynthetic pathway as a possible chemotherapeutic target whereas McConville [4] has elucidated the potential of parasite surface glycoconjugates as possible drug targets. Other targets include cysteine peptidases [5] and histone deacetylases [6] of the trypanosomatid parasites.","PeriodicalId":74217,"journal":{"name":"Molecular biology international","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3196916/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30260431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Use of antimony in the treatment of leishmaniasis: current status and future directions. 锑在治疗利什曼病中的应用:现状和未来方向。

Molecular biology international Pub Date : 2011-01-01 Epub Date: 2011-06-08 DOI: 10.4061/2011/571242

Arun Kumar Haldar, Pradip Sen, Syamal Roy

引用次数: 288

Antiproliferative, Ultrastructural, and Physiological Effects of Amiodarone on Promastigote and Amastigote Forms of Leishmania amazonensis. 胺碘酮对亚马逊利什曼原虫promastigoi型和amastigoi型的抗增殖、超微结构和生理作用。

Molecular biology international Pub Date : 2011-01-01 Epub Date: 2011-06-13 DOI: 10.4061/2011/876021

Sara Teixeira de Macedo-Silva, Thais Larissa Araújo de Oliveira Silva, Julio A Urbina, Wanderley de Souza, Juliany Cola Fernandes Rodrigues

{"title":"Antiproliferative, Ultrastructural, and Physiological Effects of Amiodarone on Promastigote and Amastigote Forms of Leishmania amazonensis.","authors":"Sara Teixeira de Macedo-Silva, Thais Larissa Araújo de Oliveira Silva, Julio A Urbina, Wanderley de Souza, Juliany Cola Fernandes Rodrigues","doi":"10.4061/2011/876021","DOIUrl":"https://doi.org/10.4061/2011/876021","url":null,"abstract":"Amiodarone (AMIO), the most frequently antiarrhythmic drug used for the symptomatic treatment of chronic Chagas' disease patients with cardiac compromise, has recently been shown to have also specific activity against fungi, Trypanosoma cruzi and Leishmania. In this work, we characterized the effects of AMIO on proliferation, mitochondrial physiology, and ultrastructure of Leishmania amazonensis promastigotes and intracellular amastigotes. The IC(50) values were 4.21 and 0.46 μM against promastigotes and intracellular amastigotes, respectively, indicating high selectivity for the clinically relevant stage. We also found that treatment with AMIO leads to a collapse of the mitochondrial membrane potential (ΔΨm) and to an increase in the production of reactive oxygen species, in a dose-dependent manner. Fluorescence microscopy of cells labeled with JC-1, a marker for mitochondrial energization, and transmission electron microscopy confirmed severe alterations of the mitochondrion, including intense swelling and modification of its membranes. Other ultrastructural alterations included (1) presence of numerous lipid-storage bodies, (2) presence of large autophagosomes containing part of the cytoplasm and membrane profiles, sometimes in close association with the mitochondrion and endoplasmic reticulum, and (3) alterations in the chromatin condensation and plasma membrane integrity. Taken together, our results indicate that AMIO is a potent inhibitor of L. amazonensis growth, acting through irreversible alterations in the mitochondrial structure and function, which lead to cell death by necrosis, apoptosis and/or autophagy.","PeriodicalId":74217,"journal":{"name":"Molecular biology international","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3200143/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30115338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 58

Nucleotide Excision Repair in Caenorhabditis elegans. 秀丽隐杆线虫的核苷酸切除修复。

Molecular biology international Pub Date : 2011-01-01 Epub Date: 2011-08-17 DOI: 10.4061/2011/542795

Hannes Lans, Wim Vermeulen

引用次数: 53

Structure and Function of the Small MutS-Related Domain. muts相关小域的结构与功能。

Molecular biology international Pub Date : 2011-01-01 Epub Date: 2011-07-19 DOI: 10.4061/2011/691735

Kenji Fukui, Seiki Kuramitsu

引用次数: 36