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A Short Historical Review of Water-Soluble Aminoplastic Resin Use for Lipid Ultrastructure Research 水溶性氨基塑料树脂用于脂质超微结构研究的历史回顾
Microscopy today Pub Date : 2023-11-01 DOI: 10.1093/mictod/qaad074
Johnson K Gao
{"title":"A Short Historical Review of Water-Soluble Aminoplastic Resin Use for Lipid Ultrastructure Research","authors":"Johnson K Gao","doi":"10.1093/mictod/qaad074","DOIUrl":"https://doi.org/10.1093/mictod/qaad074","url":null,"abstract":"Abstract In 1982, a water-soluble aminoplastic resin embedding method for electron microscopy (EM) was developed to study lipids. It involves a silylation technique for processing cured resins by converting them from hydrophilic to hydrophobic status, facilitating ultra-thin sectioning. The technique offers better preservation of lipovitellin material in frog (Rana amurensis) yolk platelets, and it shows membrane ultrastructure better than traditional epoxy embedding methods. An early (1982) pseudo-colored TEM image processing technique applied to the micrographs obtained by EM of silylated aminoplastic thin sections revealed the lipovitellin molecules, in relation to the biosynthesis of new membranes in the first cleavage of the egg, with remarkable clarity and explains the lipovitellin molecules in relation to the biosynthesis of new membranes. Because the original article was written in Chinese and published in China before its open policy, many scientists may not be aware of the publication. Thus, in this short review, the essential components of the procedure are presented in English to help guide investigators who might be interested in the use of water-soluble embedding methods for lipid research.","PeriodicalId":74194,"journal":{"name":"Microscopy today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135715344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Highlights from Microscopy and Microanalysis 显微镜和微量分析的亮点
Microscopy today Pub Date : 2023-11-01 DOI: 10.1093/mictod/qaad087
{"title":"Highlights from Microscopy and Microanalysis","authors":"","doi":"10.1093/mictod/qaad087","DOIUrl":"https://doi.org/10.1093/mictod/qaad087","url":null,"abstract":"","PeriodicalId":74194,"journal":{"name":"Microscopy today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135715339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Igniting New Confocal Imaging Potential – Nikon AX R Series with NSPARC 点燃新的共聚焦成像潜力-尼康AX R系列与NSPARC
Microscopy today Pub Date : 2023-11-01 DOI: 10.1093/mictod/qaad088
Sabrina Delattre
{"title":"Igniting New Confocal Imaging Potential – Nikon AX R Series with NSPARC","authors":"Sabrina Delattre","doi":"10.1093/mictod/qaad088","DOIUrl":"https://doi.org/10.1093/mictod/qaad088","url":null,"abstract":"Abstract The Nikon AX/AX R microscope with the recently developed Nikon Spatial Array Detector (NSPARC) provides rapid, super-resolution image acquisition of fixed and living cells. Here, we discuss the structure and function of the NSPARC, which provides excellent resolution and signal-to-noise (S/N) capabilities, and show applications related to in vitro neutrophil movement within pancreatic blood vessels and dynamic changes in mitochondrial morphology occurring over a matter of seconds. The large field-of-view and associated AI software facilitates acquisition and analysis of large 3D samples.","PeriodicalId":74194,"journal":{"name":"Microscopy today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135715077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
NetNotes 网络注释
Microscopy today Pub Date : 2023-11-01 DOI: 10.1093/mictod/qaad091
Bob Price, Bev Maleeff
{"title":"NetNotes","authors":"Bob Price, Bev Maleeff","doi":"10.1093/mictod/qaad091","DOIUrl":"https://doi.org/10.1093/mictod/qaad091","url":null,"abstract":"","PeriodicalId":74194,"journal":{"name":"Microscopy today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135715340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
PicturePuzzle PicturePuzzle
Microscopy today Pub Date : 2023-11-01 DOI: 10.1093/mictod/qaad085
{"title":"PicturePuzzle","authors":"","doi":"10.1093/mictod/qaad085","DOIUrl":"https://doi.org/10.1093/mictod/qaad085","url":null,"abstract":"","PeriodicalId":74194,"journal":{"name":"Microscopy today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135715335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dr. Lena F. Kourkoutis (1979–2023): Pioneer and Modern Role Model 莱娜·f·库尔库蒂斯博士(1979-2023):先驱和现代榜样
Microscopy today Pub Date : 2023-11-01 DOI: 10.1093/mictod/qaad084
Cameron Varano Casasanta, Bev Maleeff
{"title":"Dr. Lena F. Kourkoutis (1979–2023): Pioneer and Modern Role Model","authors":"Cameron Varano Casasanta, Bev Maleeff","doi":"10.1093/mictod/qaad084","DOIUrl":"https://doi.org/10.1093/mictod/qaad084","url":null,"abstract":"","PeriodicalId":74194,"journal":{"name":"Microscopy today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135715337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cover Art 封面
Microscopy today Pub Date : 2023-11-01 DOI: 10.1093/mictod/qaad082
{"title":"Cover Art","authors":"","doi":"10.1093/mictod/qaad082","DOIUrl":"https://doi.org/10.1093/mictod/qaad082","url":null,"abstract":"","PeriodicalId":74194,"journal":{"name":"Microscopy today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135715341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dear Abbe 亲爱的阿贝
Microscopy today Pub Date : 2023-11-01 DOI: 10.1093/mictod/qaad077
{"title":"Dear Abbe","authors":"","doi":"10.1093/mictod/qaad077","DOIUrl":"https://doi.org/10.1093/mictod/qaad077","url":null,"abstract":"","PeriodicalId":74194,"journal":{"name":"Microscopy today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135715342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Highly Integrated AFM-SEM Correlative Analysis Platform 一个高度集成的AFM-SEM相关分析平台
Microscopy today Pub Date : 2023-11-01 DOI: 10.1093/mictod/qaad083
A Alipour, K T Arat, H Alemansour, L Montes, J Gardiner, J Diederichs, B Colvin, A Amann, K Jensen, W Neils, S Spagna, L Stühn, S Seibert, H Frerichs, M Wolff, C H Schwalb
{"title":"A Highly Integrated AFM-SEM Correlative Analysis Platform","authors":"A Alipour, K T Arat, H Alemansour, L Montes, J Gardiner, J Diederichs, B Colvin, A Amann, K Jensen, W Neils, S Spagna, L Stühn, S Seibert, H Frerichs, M Wolff, C H Schwalb","doi":"10.1093/mictod/qaad083","DOIUrl":"https://doi.org/10.1093/mictod/qaad083","url":null,"abstract":"Abstract We describe the first truly correlative atomic force micro­scopy-scanning electron microscopy (AFM-SEM) platform designed from first principles and from the ground up for the study of sample properties under a wide range of magnifications. Combining these two microscopy techniques, “in situ,” into a highly integrated workstation opens unprecedented measurement capabilities at the nanoscale, while simplifying experiment workflows to yield a higher level of data throughput. Unlike SEM, the AFM offers true three-dimensional topo­graphy images, something SEM can only provide indirectly. This allows for the characterization of nano-mechanical properties, as well as for magnetic and electrical characterization of samples, which are increasingly of interest in material science, multi-component technologies (that is, solar cell and battery research), and pharmaceutical investigations. On the other hand, the SEM’s wide field-of-view is critical in identifying regions of interest with feature sizes of less than a micron, which are notoriously difficult to find over large spatial scales in conventional AFM systems. In addition, the SEM’s ability to visualize the AFM tip facilitates its navigation to aid the characterization of samples with challenging three-dimensional topographies. In this paper, we describe the major elements of the system design and demonstrate how correlative microscopy can help the characterization of samples with challenging morphologies such as the edge of a razor blade or the nanomechanical analysis of platinum nanopillars.","PeriodicalId":74194,"journal":{"name":"Microscopy today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135715076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Microscopy Helps Reveal How Benjamin Franklin Foiled Counterfeiters 显微镜有助于揭示本杰明·富兰克林如何挫败造假者
Microscopy today Pub Date : 2023-11-01 DOI: 10.1093/mictod/qaad079
Stephen W Carmichael
{"title":"Microscopy Helps Reveal How Benjamin Franklin Foiled Counterfeiters","authors":"Stephen W Carmichael","doi":"10.1093/mictod/qaad079","DOIUrl":"https://doi.org/10.1093/mictod/qaad079","url":null,"abstract":"","PeriodicalId":74194,"journal":{"name":"Microscopy today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135715336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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