Igniting New Confocal Imaging Potential – Nikon AX R Series with NSPARC

Abstract The Nikon AX/AX R microscope with the recently developed Nikon Spatial Array Detector (NSPARC) provides rapid, super-resolution image acquisition of fixed and living cells. Here, we discuss the structure and function of the NSPARC, which provides excellent resolution and signal-to-noise (S/N) capabilities, and show applications related to in vitro neutrophil movement within pancreatic blood vessels and dynamic changes in mitochondrial morphology occurring over a matter of seconds. The large field-of-view and associated AI software facilitates acquisition and analysis of large 3D samples.