African Journal of Biotechnology最新文献_第7页

The effect of foliar application of zinc oxide nanoparticles and Moringa oleifera leaf extract on growth, biochemical parameters and in promoting salt stress tolerance in faba bean 叶面施用氧化锌纳米颗粒和辣木叶提取物对蚕豆生长、生化参数及提高耐盐性的影响

African Journal of Biotechnology Pub Date : 2022-06-30 DOI: 10.5897/ajb2022.17485

Sherif M. Ragab, L. Turoop, S. Runo, S. Nyanjom

{"title":"The effect of foliar application of zinc oxide nanoparticles and Moringa oleifera leaf extract on growth, biochemical parameters and in promoting salt stress tolerance in faba bean","authors":"Sherif M. Ragab, L. Turoop, S. Runo, S. Nyanjom","doi":"10.5897/ajb2022.17485","DOIUrl":"https://doi.org/10.5897/ajb2022.17485","url":null,"abstract":"Salinity is major abiotic stress limiting plant growth worldwide. Plant adaptation to salinity stress involves diverse physiological and metabolic pathways. In this study, we assessed the effects of foliar application of zinc oxide nanoparticles (ZnONPs) and Moringa leaf extract (MLE) on salt tolerance in faba beans (cultivar, Sakha 4). Morphological, chemical, and biochemical parameters of plants grown under saline condition (50 and 100 mM NaCl) were assessed 60 days after sowing. Salt stress caused a remarkable reduction in growth traits, photosynthetic pigments, proline, minerals, total phenol, and enzyme activity of the faba bean variety. The results showed that foliar spraying of MLE and ZnONPs on faba bean grown under salt-stressed conditions promoted growth parameters (that is, shoot length, numbers of leaves, relative water content, shoot, roots fresh and dry weights), photosynthetic pigments (that is, chl a, b, total chlorophyll and carotenoids), proline, mineral elements (Na + , K + , Ca +2 , and Zn +2 ), total phenol and enzyme activity (POX, PPO, APX, and CAT) compared to control plants. Based on these findings, the potential of foliar spraying application of MLE and ZnONPs may help alleviate the negative effect of salinity on growth, photosynthesis efficiency, and biochemical properties of faba bean .","PeriodicalId":7414,"journal":{"name":"African Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41713449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Preliminary verification of the adoption status of some yam (Dioscorea rotundata and Dioscorea alata) varieties in Nigeria using microsatellites markers 利用微卫星标记初步验证尼日利亚部分薯蓣（圆叶薯蓣和有叶薯蓣）品种的引种状况

African Journal of Biotechnology Pub Date : 2022-05-31 DOI: 10.5897/ajb2021.17425

J. Nwogha, J. Obidiegwu, R. N. Okereke, R. Bhattacharjee, H. Oselebe

{"title":"Preliminary verification of the adoption status of some yam (Dioscorea rotundata and Dioscorea alata) varieties in Nigeria using microsatellites markers","authors":"J. Nwogha, J. Obidiegwu, R. N. Okereke, R. Bhattacharjee, H. Oselebe","doi":"10.5897/ajb2021.17425","DOIUrl":"https://doi.org/10.5897/ajb2021.17425","url":null,"abstract":"The persistent low yield and farmers’ preference of their traditional yam varieties over the improved varieties necessitated this study to verify the adoption status of the released varieties in Nigeria. A total of 48 accessions of white yam ( Dioscorea rotundata ) were sampled from six states of Ebonyi, Enugu, Benue, Kogi, Nassarawa and Oyo within Nigeria yam-belt and were genotyped for relatedness to four released varieties from the National Root Crops Research Institute (NRCRI), Umudike yam breeding programme, while 14 accessions of water yam ( D. alata ) were sampled from four states of Benue, Kogi, Nassarawa and Oyo and were also genotyped for relatedness to three released varieties from International Institute for Tropical Agriculture (IITA), Ibadan. A total of 29 alleles were found in 5 sets of primers analyzed for 52 D. rotundata accessions and the number of alleles ranged from 5 (Dald08, SSR 51 and YM 34) to 8 (Dab2E07) with an average of 5.8 per locus. The observed heterozygosity ranged from 0.19 (YM34) to 0.77 (YM30). A total gene diversity of 0.63 according to Nei (1978) genetic distance coefficients was observed among the 52 D. rotundata accessions. Similarly, a total of 37 alleles were observed when 17 D. alata accessions were analysed with the 7 selected sets of primers. An average of 5.29 alleles was observed per locus. The observed heterozygosity varied from 0.47 (Dab2D06) to 0.82 (YM34). A total gene diversity of 0.58 was observed among 17 D. alata accessions according to Nei’ genetic distance coefficients. Cluster analysis showed that the D. rotundata accessions were classified into 8 clusters. While, 17 accessions of D. alata were classified into 4 clusters. There were relationships between some released varieties and farmers accessions and also among the farmers’ accessions from different locations, indicating that farmers might have given a preferred local name to the released varieties.","PeriodicalId":7414,"journal":{"name":"African Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49578317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Efficient callus-mediated system for commercial production of sugarcane (Saccharum spp.) planting material in Ghana 加纳甘蔗种植材料商业化生产的高效愈伤组织介导系统

African Journal of Biotechnology Pub Date : 2022-05-31 DOI: 10.5897/ajb2021.17440

E. Azu, W. Elegba, Abigail Tweneboah Asare, Kwame Kumi Asare, C. Akama, Patience Asare, C. Annor, Samuel Azure, K. Danso

{"title":"Efficient callus-mediated system for commercial production of sugarcane (Saccharum spp.) planting material in Ghana","authors":"E. Azu, W. Elegba, Abigail Tweneboah Asare, Kwame Kumi Asare, C. Akama, Patience Asare, C. Annor, Samuel Azure, K. Danso","doi":"10.5897/ajb2021.17440","DOIUrl":"https://doi.org/10.5897/ajb2021.17440","url":null,"abstract":"An efficient callus-mediated regeneration system was developed for high-frequency production of planting material of sugarcane genotypes LSC and B36464. Spindle leaf segments cultured on MS basal medium supplemented with 2,4-D or picloram at 1, 2, 3 or 4 mg/L resulted in callus induction. Callus induction was higher on 2,4-D amended medium compared to picloram. Nevertheless, for both auxins, callus induction improved significantly (p ≤ 0.05) with increasing concentration; the highest (82 and 82.5% for B36464 and LSC respectively) was achieved at 4 mg/L. For shoot induction, calli were transferred to MS medium supplemented with BAP (0.1, 0.5, 1.0 or 1.5 mg/L). The highest number of shoots (18.13 and 16.75 for B36464 and LSC respectively) was achieved at 1.5 mg/L. Serial subculture at four-week intervals on a higher concentration of BAP (2.5 mg/L), in combination with NAA (0.5 mg/L) and GA3 (0.5 mg/L), resulted in a four-fold increase in shoot number within 16 weeks. On this medium, 40% of shoot clusters of B36464 formed well-defined shoots. On MS medium containing solely NAA (3 mg/L), 88 and 72% (B36464 and LSC respectively) formed roots. Post-flask acclimatisation of the plantlets led to 85 and 91% survival rates in LSC and B36464 respectively after which plantlets were successfully transferred to field conditions. The callus-mediated regeneration system reported in this study has the potential to sustainably provide sugarcane planting material for the emerging sugar industry in Ghana.","PeriodicalId":7414,"journal":{"name":"African Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43944882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization and antimicrobial activities of lactic acid bacteria isolated from selected Nigerian traditional fermented foods 尼日利亚传统发酵食品中乳酸菌的鉴定及抑菌活性研究

African Journal of Biotechnology Pub Date : 2022-05-31 DOI: 10.5897/ajb2021.17450

C. Ohaegbu, A. Ngene, Unyime Inyang Asuquo, O. D. Coulthard, E. Nwachukwu

{"title":"Characterization and antimicrobial activities of lactic acid bacteria isolated from selected Nigerian traditional fermented foods","authors":"C. Ohaegbu, A. Ngene, Unyime Inyang Asuquo, O. D. Coulthard, E. Nwachukwu","doi":"10.5897/ajb2021.17450","DOIUrl":"https://doi.org/10.5897/ajb2021.17450","url":null,"abstract":"For production and preservation of traditional fermented foods, the genera, lactic acid bacteria (LAB) have been used. This study was carried out to determine the characteristics and the antimicrobial activities of lactic acid bacteria isolated from selected Nigerian traditional fermented foods. Changes in pH and titratable acidity (TA) of the samples were investigated for a period of four days (96 h). Eleven tentative LAB from fermented maize and cassava (Ogi and Fufu, respectively) were isolated and characterized. The spoilage organisms from fish were aseptically identified and the antimicrobial activity was determined by agar well diffusion method against three isolated food spoilage organisms (Pseudomonas aeruginosa, Enterobacter aerogene and Bacillus cereus). The isolates were selected and further identified as Lactobacillus amylolyticus strain L6, Lactobacillus plantarum strain ci-4w and Lactobacillus sakei strain MLS1 by the aide of genotypic characteristics (16S rRNA gene sequences). These strains were screened for their EPS producing activity, resistance to low pH and bile salts as well as bacteriocin activity. These strains can be used as starter culture or protective cultures to improve the hygiene, quality and increased safety of the food products by inhibiting the food borne pathogens and spoilage microorganisms.","PeriodicalId":7414,"journal":{"name":"African Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46668544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Commercialization of genetically modified crops in Africa: Opportunities and challenges 非洲转基因作物商业化：机遇与挑战

African Journal of Biotechnology Pub Date : 2022-05-31 DOI: 10.5897/ajb2021.17434

G. Endale, Maredia Karim, Guenthner Joseph, Koch Muffy

{"title":"Commercialization of genetically modified crops in Africa: Opportunities and challenges","authors":"G. Endale, Maredia Karim, Guenthner Joseph, Koch Muffy","doi":"10.5897/ajb2021.17434","DOIUrl":"https://doi.org/10.5897/ajb2021.17434","url":null,"abstract":"Genetically modified (GM) crops offer potential for enhancing agricultural productivity for smallholder farmers in Africa. After nearly three decades of research and development collaboration and regulatory capacity strengthening, several countries in Sub-Saharan Africa (SSA) are moving towards commercializing GM crops for the benefit of smallholder farmers. South Africa approved genetically modified (GM) cotton, maize and soybeans in the 1990s. Nigeria, Ethiopia, Kenya, Sudan, Eswatini and Malawi recently approved general release of GM crops, including GM cotton, GM cowpea, GM maize, and GM cassava through public-private partnerships. Collected data from a diverse group of 30 stakeholders from 14 countries in Africa and results indicated that while progress has been made towards commercializing GM crops in several countries in Africa, some key challenges and downstream issues remain to be addressed. These include building functional regulatory systems, vibrant seed systems, local seed production, effective extension services, reliable credit/financial and marketing services, and improved access to markets for smallholder farmers. Unless these downstream issues are effectively addressed, smallholder farmers in Africa will not benefit from GM crops. questions (160 questions) raised by stakeholders that attended the short courses. The questions were recorded and categorized into representative themes: product development, regulation, technology transfer (including IP, licensing, scaling up, seed systems), communication and outreach, public acceptance and trade to understand stakeholders areas of concern. The information included in this paper is part of a needs assessment survey on biotechnology and biosafety development, level of awareness of advances in the biotech product commercialization and genome editing technologies in developing countries, as well as the challenges faced and capacity building needs for commercialization and adoption of GM crops","PeriodicalId":7414,"journal":{"name":"African Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44018809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

New technique for improving fish packaging hygiene and prolonged shelf life 提高鱼品包装卫生和延长保质期的新技术

African Journal of Biotechnology Pub Date : 2022-05-31 DOI: 10.5897/ajb2022.17467

N. Elsharawy, Wafa A. Baabdullah, A. Alkaladi

{"title":"New technique for improving fish packaging hygiene and prolonged shelf life","authors":"N. Elsharawy, Wafa A. Baabdullah, A. Alkaladi","doi":"10.5897/ajb2022.17467","DOIUrl":"https://doi.org/10.5897/ajb2022.17467","url":null,"abstract":"Zinc oxide nanoparticles (ZnO NPs) have received great attention due to their optical, physical, and antimicrobial properties. They have toxic effect against microbes without any effect on mammalians cells. They are used in several applications including extending the shelf life of food. The study aims to determine the minimum inhibitory concentrations of ZnO NPs against different aquaculture fish fungus species and their storage period. A total of 160 samples were collected from different types of aquaculture fish samples as follows: rabbitfish, bream, red mullet, saddle grouper, spangled emperor, gilthead seabream, mackerel fish, and Asian seabass. ZnO NPs activity against the isolated fungus species was evaluated by estimating minimum fungicidal inhibitory concentration and inhibition of fungal enzymes (amylase, protease, and lipase). The storage period of the fish in a package containing ZnO NPs was determined by estimating the sensory characteristics of the treated fish. The results obtained recorded the following fungus species from aquaculture fish samples: Aspergillus niger (gi: JX112703), Aspergillus oryzae, Aspergillus awamori, Penicillium species, Aspergillus tubingensis, Trichosporon montevideense, A. niger (gi: MG889596), and Byssochlamys spectabilis, respectively. This study is the first to apply ZnO NPs for fish preservation which have a powerful antifungal effect against all the isolated fungi. The study recommends using 3% ZnO NPs in fish packaging film; it inhibited most of the fungus species, extending the shelf life of most of the fish species to more than 15 days.","PeriodicalId":7414,"journal":{"name":"African Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45766630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization of Escherichia co-resistance genes from chicken meat 鸡肉中埃希氏菌共抗基因的分子特征分析

African Journal of Biotechnology Pub Date : 2022-04-30 DOI: 10.5897/ajb2022.17453

T. E. Nagwa, A. Hind, A. Amr

{"title":"Molecular characterization of Escherichia co-resistance genes from chicken meat","authors":"T. E. Nagwa, A. Hind, A. Amr","doi":"10.5897/ajb2022.17453","DOIUrl":"https://doi.org/10.5897/ajb2022.17453","url":null,"abstract":"Escherichia coli multi-resistance to a variety of antimicrobials is a result of gene mutation on plasmids, integrons and transposons. The aims of this work were to: (1) detect genotype and phenotype antibiotic resistance genes in E. coli , and (2) determine whole-genome sequencing to discover E. coli gene multidrug resistance in chicken meat. Samples were gathered, processed, and analysed bacteriologically; thereafter an antimicrobial sensitivity test was performed and E. coli isolates were identified serologically. Results of E. coli were 40% from 100 chicken samples. The most potent antibiotics against E. coli were Cephalosporins, Quinolones and Oxytetracycline. The serological investigation was as follows: 30% (O157:H7) of STEC, 30% (O142) of ETEC, 10% (O26:H11) of EHEC and 10% EPEC. Subunit B of Shiga-like toxin (SLT) gene showed a symmetrical band, while, Heat-labile toxin (LT) gene was estimated in both plasmid preps in addition to DNA genomic strains. STEC is hazardous to the chicken meat consumers. The study recommended necessary improvement in the hygienic procedures during all processing steps, and minimized the non-important usage of antibiotics to prevent antibiotics resistant.","PeriodicalId":7414,"journal":{"name":"African Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43861716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of PCR protocol for ISSR marker based genetic diversity assessment of acid lime [Citrus aurantifolia (Christm.) Swingle] germplasm in Eastern Nepal 基于ISSR标记的尼泊尔东部酸橙种质遗传多样性评价PCR方案的优化

African Journal of Biotechnology Pub Date : 2022-04-30 DOI: 10.5897/ajb2021.17427

Narayan Munankarmi Nabin, Rana Neesha, Bhattarai Tribikram, L. Ram, Chaudhary Sujan, Shrestha Sangita

{"title":"Optimization of PCR protocol for ISSR marker based genetic diversity assessment of acid lime [Citrus aurantifolia (Christm.) Swingle] germplasm in Eastern Nepal","authors":"Narayan Munankarmi Nabin, Rana Neesha, Bhattarai Tribikram, L. Ram, Chaudhary Sujan, Shrestha Sangita","doi":"10.5897/ajb2021.17427","DOIUrl":"https://doi.org/10.5897/ajb2021.17427","url":null,"abstract":"Acid lime [ Citrus aurantifolia (Christm.) Swingle] is a valuable commercial fruit crop grown in Nepal's Terai to high hills which has high economic, cultural and medicinal importance. Due to low quality planting materials and poor orchard management, production and productivity of acid lime are extremely low in Nepal. The present study aimed at optimization of Inter-Simple Sequence Repeat (ISSR)-polymerase chain reaction (PCR) reaction and cycling conditions for PCR amplification and genetic diversity assessment of acid lime cultivars from eastern agro-ecological zone, Nepal. Five different parameters [ viz. Template DNA, MgCl 2 , Deoxynucleotide triphosphate (dNTPs), Primers and Taq DNA polymerase] were used in the ISSR-PCR reaction optimization. Moreover, 4 different cycling conditions were assessed for the determination of the optimum range for ISSR-PCR profiling. The optimized PCR reaction conditions were found to be 25 ng DNA, 3.0 mM MgCl 2 , 0.4 mM dNTPs, 0.4 µM Primers and 1.5 Unit Taq DNA polymerase and best PCR cycling condition consisted of initial denaturation of 94°C for 5 min followed by 40 cycles of denaturation at 94°C for 30 s, annealing at 50°C for 45 s, elongation at 72°C for 2 min and final elongation of 7 min at 72°C. The results from this study were successfully used for ISSR-PCR based genetic diversity assessment of Nepalese acid lime genotypes to find out the elite cultivars of Eastern Nepal.","PeriodicalId":7414,"journal":{"name":"African Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45768597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Metabolic engineering of Neurospora crassa for increasing carotenoids synthesis 粗脉孢菌的代谢工程提高类胡萝卜素的合成

African Journal of Biotechnology Pub Date : 2022-04-30 DOI: 10.5897/ajb2021.17442

E. B. Sylvia, Lin Chun, Liu Zhengjie, Wen-Bo Hao, C. Qin, Mao Zichao

{"title":"Metabolic engineering of Neurospora crassa for increasing carotenoids synthesis","authors":"E. B. Sylvia, Lin Chun, Liu Zhengjie, Wen-Bo Hao, C. Qin, Mao Zichao","doi":"10.5897/ajb2021.17442","DOIUrl":"https://doi.org/10.5897/ajb2021.17442","url":null,"abstract":"Carotenoids are essential nutrient compounds with numerous biological functions. Neurospora crassa is a model filamentous fungus with orange pigmentation which is attributed to the accumulation of carotenoids containing neurosporaxanthin (NX) and neutral carotenoids (NC). To enhance carotenoids synthesis in N. crassa , isoprene diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) were increased using the genes, xylulose-5-phosphate phosphoketolase ( XPK ), phosphotransacetylase ( PTA ), and NADH-specific-3-hydroxy-3-methyl glutaryl coenzyme A reductase ( HMGR ), as single, fused and three combined expressions to channel more carbon source into the mevalonate pathway (MVP). The single ( PTA , XPK , HMGR ), fused ( PTA:HMGR & XPK:HMGR ) and three combined gene ( PTA with fused XPK:HMGR ) expressions in engineered fungal resulted in carotenoid titers with contents of NX accumulated up to 4.5 mg/g DW and NC up to 1.7 mg/g DW as compared to the wild-type with NX up to 1.54 mg/g DW and NC up to 0.8 mg/g DW. The optimized MVP with metabolic engineering methods is a key method to increase the synthesis of carotenoid and other active terpenoids crassa .","PeriodicalId":7414,"journal":{"name":"African Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49016919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy of Sudanese isolates of entomopathogenic fungi against the Khapra beetle Trogoderma granarium (Everts) (Coleoptera: Dermestidae) 苏丹分离的昆虫病原真菌对卡普拉甲虫(鞘翅目:皮蝇科)的防治效果

African Journal of Biotechnology Pub Date : 2022-04-30 DOI: 10.5897/ajb2021.17420

Mohammed Ali Hammad Ahmed, Abdelkareem Geddo Abdelkareem Adam, Omer Abdelbagi Azhari, Elaziz Sulieman Ahmed Ishag Abd, Delmege Laing Mark, Hur Jang-Hyun

{"title":"Efficacy of Sudanese isolates of entomopathogenic fungi against the Khapra beetle Trogoderma granarium (Everts) (Coleoptera: Dermestidae)","authors":"Mohammed Ali Hammad Ahmed, Abdelkareem Geddo Abdelkareem Adam, Omer Abdelbagi Azhari, Elaziz Sulieman Ahmed Ishag Abd, Delmege Laing Mark, Hur Jang-Hyun","doi":"10.5897/ajb2021.17420","DOIUrl":"https://doi.org/10.5897/ajb2021.17420","url":null,"abstract":"The current study evaluated the bio-control activity of Sudanese isolates of entomopathogenic-fungi against 3 rd larval instars of Khapra beetle ( Trogoderma granarium ) (Everts) in Sudan and morphologically and molecularly characterized the virulent isolates. Fungi were isolated using Galleria-baiting method and tested against the larvae using immersion-technique at concentration of 1×10 7 conidia ml -1 . Commercial product, Eco-Bb® was used as standard treatment. Twenty of the 3 rd instar larvae were immersed in 10 ml of fungal suspension for five seconds. Control larvae were immersed in sterilized-distilled water. Dead insects were counted daily for seven days after inoculation. Microscopic examination of the cadavers was conducted to explain whether or not the test organisms caused the death of test larvae. Virulent isolates were identified morphologically and confirmed by molecular techniques as Beauveria bassiana isolate Sud-afro.18 (MK046654), Metarhizium anisopliae isolate Dmazeen F1 (MK046658), Metarhizium anisopliae isolate Dmazeen R1 (MK046659), Albifimbria viridis isolate Shmbat-fo1 (MK046656), Purpureocillium lilacinum isolate Khartoum f1 (MK046655), B. bassiana isolate Sud-afro.20 (MK046652), B. bassiana isolate Giddo6RF (MN598664), B. bassiana isolate GiddoR (MN598665), and B. bassiana isolate HammadR7,F (MN598666). Mortality induced by various isolates ranged from 40.0-90.4% compared to 96 and 7.9% induced by standard treatment Eco-Bb® and untreated control respectively. LT 50 values of B. bassiana isolate Sudafro.18 and M. anisopliae isolate DmazeenF1 is comparable to that induced by Eco-Bb®.","PeriodicalId":7414,"journal":{"name":"African Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46004418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1