American Journal of Biochemistry and Biotechnology最新文献_第6页

Phosphorylation, Characterization, and Antioxidant Activity of Polysaccharides from Flammulina Velutipes Scraps 金针菇多糖的磷酸化、表征及抗氧化活性研究

American Journal of Biochemistry and Biotechnology Pub Date : 2022-03-01 DOI: 10.3844/ajbbsp.2022.279.288

Yingyun Peng, Xue Bai, Hanwen Xu, Yuan Jin, Yufeng Wu, Yiyong Chen

引用次数: 0

Preparation, Characterization and Antioxidant Activity of a Novel Acetylated Polysaccharide from Flammulina Velutipes 金针菇乙酰化多糖的制备、表征及抗氧化活性研究

American Journal of Biochemistry and Biotechnology Pub Date : 2022-03-01 DOI: 10.3844/ajbbsp.2022.339.348

Yingyun Peng, Han Jiang, Yufeng Wu, Jinlong Zhang, Jianhua Zhou, Yiyong Chen

{"title":"Preparation, Characterization and Antioxidant Activity of a Novel Acetylated Polysaccharide from Flammulina Velutipes","authors":"Yingyun Peng, Han Jiang, Yufeng Wu, Jinlong Zhang, Jianhua Zhou, Yiyong Chen","doi":"10.3844/ajbbsp.2022.339.348","DOIUrl":"https://doi.org/10.3844/ajbbsp.2022.339.348","url":null,"abstract":": In this study, a novel polysaccharide FVP2a was extracted and purified from F. velutipes. Ac-FVP2a was prepared by chemical acetylation. The preparation process of Ac-FVP2a was optimized using the response surface method based on three experimental factors including reaction time, reaction temperature, and the ratio of FVP2a to acetic anhydride. The physicochemical characteristics and antioxidant activities of Ac-FVP2a in vitro were investigated. The results showed that the optimal preparation process of Ac-FVP2a was a reaction time of 2.5 h, reaction temperature of 45℃ and ratio of FVP2a to acetic anhydride of 1:37 (g/mL), respectively. The monosaccharides of Ac-FVP2a with an average molecular weight of 1.97 × 10 5 Da were galactose, glucose, rhamnose, xylose, and arabinose and the molar ratio were 1.65:1:0.13:0.26:1.12. FT-IR suggested that Ac-FVP2a was prepared successfully. Ac-FVP2a had stronger antioxidant activities with better thermal stability than FVP2a. Ac-FVP2a might have the potential to become a novel antioxidant in the food industry.","PeriodicalId":7412,"journal":{"name":"American Journal of Biochemistry and Biotechnology","volume":"72 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85530272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nuclear Factor Erythroid-2-Related Factor 2 (Nrf2) Signalling Pathway and Apoptosis Impact on the Effect of Tetramethylpyrazine against H2O2-Damage in Human Hepatic Cell Line (LO2 Cells) 核因子-红细胞-2相关因子-2 (Nrf2)信号通路及凋亡对川芎嗪抗h2o2损伤作用的影响

American Journal of Biochemistry and Biotechnology Pub Date : 2022-03-01 DOI: 10.3844/ajbbsp.2022.308.318

Songbo Guo, Xin-yue Han, Shanbin Chen, Xiaomeng Zhang, Hongjiao Li, Yishu Li, Wenmei Zhao, Liaofei Zhang, B-B. Sun, Feike Hao, Yefu Chen

{"title":"Nuclear Factor Erythroid-2-Related Factor 2 (Nrf2) Signalling Pathway and Apoptosis Impact on the Effect of Tetramethylpyrazine against H2O2-Damage in Human Hepatic Cell Line (LO2 Cells)","authors":"Songbo Guo, Xin-yue Han, Shanbin Chen, Xiaomeng Zhang, Hongjiao Li, Yishu Li, Wenmei Zhao, Liaofei Zhang, B-B. Sun, Feike Hao, Yefu Chen","doi":"10.3844/ajbbsp.2022.308.318","DOIUrl":"https://doi.org/10.3844/ajbbsp.2022.308.318","url":null,"abstract":": As an active component in traditional Chinese herbal medicine, Tetramethylpyrazine (TMP) played a critical role in the treatment of cardiovascular, cerebrovascular, and eye diseases. In this study, the TMP has obtained from the Chinese herbal medicine Ligusticum chuanxiong and investigated the protective effects of TMP on oxidative damage in human LO 2 cells with the treatment of different concentrations of TMP. The results showed that TMP reserved oxidative damage caused by hydrogen peroxide in LO 2 cells. Further other investigations suggested that TMP protected LO 2 cells from oxidative damage through elevating activities of antioxidant enzymes and glutathione levels. The TMP treatment promoted the nucleus translocation of the nuclear factor erythroid 2–related factor 2 (Nrf2) and further induced expression of antioxidant response element. Finally, it could be concluded that TMP treatment suppressed apoptosis induced by hydrogen peroxide in LO 2 cells. Thus, the TMP might protect LO 2 cells from oxidative damage through antioxidative and anti-apoptotic effects.","PeriodicalId":7412,"journal":{"name":"American Journal of Biochemistry and Biotechnology","volume":"110 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75740077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of Rotten Fresh-Cut Cauliflowers based on Machine Vision Technology and Watershed Segmentation Method 基于机器视觉和分水岭分割的腐鲜菜花检测

American Journal of Biochemistry and Biotechnology Pub Date : 2022-02-01 DOI: 10.3844/ajbbsp.2022.155.167

Jianxin Xue, Liang Huang, Bingyu Mu, Kai Wang, Zihui Li, Haixia Sun, Huamin Zhao, Zezhen Li

{"title":"Detection of Rotten Fresh-Cut Cauliflowers based on Machine Vision Technology and Watershed Segmentation Method","authors":"Jianxin Xue, Liang Huang, Bingyu Mu, Kai Wang, Zihui Li, Haixia Sun, Huamin Zhao, Zezhen Li","doi":"10.3844/ajbbsp.2022.155.167","DOIUrl":"https://doi.org/10.3844/ajbbsp.2022.155.167","url":null,"abstract":": In this study, machine vision technology was used to separate the samples and detect the rotting degrees of fresh-cut cauliflowers. First, the improved watershed algorithm was used for the segmentation of fresh-cut cauliflower samples and the extraction of single-sample. Then, three-color models, a gray co-occurrence matrix, and two feature extraction algorithms were used to extract the color, texture, and spectral feature parameters of the images. At the same time, the Partial Least Squares Discriminant Analysis (PLS-DA) and Extreme Learning Machines (ELM) discriminant models were established. The identification accuracy of PLS-DA and ELM discriminant models for rotting samples was 95 and 90.9%, respectively. Moreover, according to the size of rotten areas, the rotting grades were divided and the contours and feature areas of rotten cauliflower samples were identified by the region growth algorithm and the “Sobel” operator. Finally, the detection and identification of the rotting degree of cauliflower samples were realized. The results showed that machine vision technology can segment the cohesive fresh-cut cauliflower samples and can be used for qualitative and quantitative identification of the intact and rotten cauliflower","PeriodicalId":7412,"journal":{"name":"American Journal of Biochemistry and Biotechnology","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85498368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Different Arrangement of Dopamine Receptors/NMDA Receptors Heterocomplexes in the Brain Regions of a Healthy Male, Female and Audiogenic Seizure-Prone Male Rats 多巴胺受体/NMDA受体异复合体在健康雄性、雌性和听原性癫痫易发雄性大鼠脑区的不同排列

American Journal of Biochemistry and Biotechnology Pub Date : 2022-02-01 DOI: 10.3844/ajbbsp.2022.195.204

G. Tevzadze, E. Zhuravliova, Natia Okriashvili, N. Narmania, T. Barbakadze, D. Mikeladze

{"title":"Different Arrangement of Dopamine Receptors/NMDA Receptors Heterocomplexes in the Brain Regions of a Healthy Male, Female and Audiogenic Seizure-Prone Male Rats","authors":"G. Tevzadze, E. Zhuravliova, Natia Okriashvili, N. Narmania, T. Barbakadze, D. Mikeladze","doi":"10.3844/ajbbsp.2022.195.204","DOIUrl":"https://doi.org/10.3844/ajbbsp.2022.195.204","url":null,"abstract":": Differences in the composition and subcellular localization of heteroreceptors in the brain can trigger central nervous system diseases, including epilepsy and autism. Protein-protein interactions between Dopamine Receptors (DRs) and GLUN2A and GLUN2B subunits are critical mechanisms that regulate dopamine and glutamate coordinated signals. These interactions may be involved in the pathophysiological predisposition of epilepsy. We hypothesized that the specificity of dopaminergic neurotransmission in audiogenic Seizure-Prone (SP) rats could be underlined by the distribution and expression of the N-Methyl-D-Aspartate Glutamate Receptor (NMDAR) and Dopamine Receptor (NMDAR/DR) heterocomplex. We determined the oligomerization of synaptic and extra-synaptic NMDAR subunits with D1 and D2 receptors in the prefrontal cortex and Nucleus Accumbens (NAc) of healthy and epileptic males rats. Considering that the comorbidity of epilepsy and autism is more prevalent in females, we also studied the pattern of interaction. in NMDAR/DR heterocomplex formation in healthy female rats. The association of Dopamine Receptor type 1 (D1R) with the GLUN2A receptor, in the extra-synaptic fraction of epilepsy-prone rats, was lower than that in healthy female and male rats. In contrast, the interaction of D1R with the GLUN2B receptor was higher in the extra-synaptic membranes of epilepsy-prone rats than that in healthy rats. Furthermore, we found that the D2R/GLUN2B ratio was higher in the synaptic NAc fraction of SP and female rats. Therefore, we suggest that the different subunit proportions of the NMDAR/DR heterocomplex in the prefrontal cortex and NAc of male, female, and SP rats can be attributed to their cognitive or emotional flexibility.","PeriodicalId":7412,"journal":{"name":"American Journal of Biochemistry and Biotechnology","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81907257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Automatic Diagnosis of Soybean Leaf Disease by Transfer Learning 基于迁移学习的大豆叶片病害自动诊断

American Journal of Biochemistry and Biotechnology Pub Date : 2022-02-01 DOI: 10.3844/ajbbsp.2022.252.260

Xiao Yu, Qi Gong, Cong Chen, Lina Lu

引用次数: 2

Acer Buergerianumas a New Resource for the Extraction of Corilagin: Determination of Corilaginin Different Cultivars of A. Buergerianumby UPLC 棉毛槭:提取棉毛蛋白的新资源——棉毛槭不同品种棉毛蛋白的UPLC测定

American Journal of Biochemistry and Biotechnology Pub Date : 2022-02-01 DOI: 10.3844/ajbbsp.2022.205.212

Qian Qiao, Jiangyong Wang, A. Zhang, Jihan Tao, Zhen Feng, Yu Yan, Lin Zhang, Yi Lu

{"title":"Acer Buergerianumas a New Resource for the Extraction of Corilagin: Determination of Corilaginin Different Cultivars of A. Buergerianumby UPLC","authors":"Qian Qiao, Jiangyong Wang, A. Zhang, Jihan Tao, Zhen Feng, Yu Yan, Lin Zhang, Yi Lu","doi":"10.3844/ajbbsp.2022.205.212","DOIUrl":"https://doi.org/10.3844/ajbbsp.2022.205.212","url":null,"abstract":": Corilagin is a promising anticancer drug candidate. Acer nikoense and A. ammonium are both known to contain corilagin. To explore whether corilagin occurs in congeneric species and to fully develop the medicinal value of maple, the corilagin content in different varieties of maple was determined by Ultrahigh-Performance Liquid Chromatography (UPLC). Acer buergerianum leaves contained corilagin and the corilagin content in leaves of different varieties at the deciduous stage varied from 1.2371 to 6.8887 mg/g, with an average of 4.3791 mg/g. Among the cultivars, ‘Xingwang’ and ‘Qianlihong' showed relatively high corilagin contents and the content in ‘Qilujin’ was the lowest. The range of corilagin contents at the new leaf stage was 8.7452~11.2441 mg/g. Many parts of A. buergerianum contained corilagin, with the highest content in the leaves; based on this considerable corilagin concentration, this plant can be developed into raw material for corilagin extraction. There are large differences among varieties. To increase the output value and efficiency, it is necessary to further screen high-content varieties for industrial production; harvesting can be performed in spring and autumn, with autumn as the main season and spring as a supplemental season.","PeriodicalId":7412,"journal":{"name":"American Journal of Biochemistry and Biotechnology","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77438999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatial Distribution and Evaluation of Heavy Metal Pollution in Soil Based on Three-Dimensional Model and Kriging Interpolation 基于三维模型和Kriging插值的土壤重金属污染空间分布与评价

American Journal of Biochemistry and Biotechnology Pub Date : 2022-02-01 DOI: 10.3844/ajbbsp.2022.224.237

Baoshun Liu, Yonghong Zhang, Jun Yao, J. Su

{"title":"Spatial Distribution and Evaluation of Heavy Metal Pollution in Soil Based on Three-Dimensional Model and Kriging Interpolation","authors":"Baoshun Liu, Yonghong Zhang, Jun Yao, J. Su","doi":"10.3844/ajbbsp.2022.224.237","DOIUrl":"https://doi.org/10.3844/ajbbsp.2022.224.237","url":null,"abstract":"Corresponding Author: Yonghong Zhang Department of Civil and Resource Engineering, University of Science and Technology Beijing, Beijing 100083, China Email: yhzhang0623@163.com Abstract: Soil heavy metal pollution is an important content for soil environmental governance. Based on the three-Dimensional (3D) geological modeling technology and ordinary Kriging method, this study described and analyzed the soil pollution status and spatial distribution characteristics of a site in Zhongshan, Guangxi. The results showed that combining 3D geological model and the Kriging interpolation method can well evaluate soil heavy metal pollution. The 3D geological model showed that the pollutant concentration in soil ranked As>Pb>Mo and the pollution degree decreased gradually from top to bottom. As such, the miscellaneous fill soil layer was the layer that needs to be repaired. In addition, the single-factor pollution level and Nemerow comprehensive index method were used to evaluate the pollution risk of the site. The evaluation results showed that the soil quality was seriously threatened by As and the heavy pollution (Pi>3) accounted for 61.10%. Furthermore, attention should be paid to the potential risk of Pb in the miscellaneous fill layer. This study can provide a 3D visualization method for comprehensive analysis of soil heavy metal pollution and a scientific reference for the formulation and implementation of subsequent site remediation strategies.","PeriodicalId":7412,"journal":{"name":"American Journal of Biochemistry and Biotechnology","volume":"74 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74309207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of the Wnt/β-Catenin Signaling Pathway in Reducing Lipogenesis of Obese Rats Under Hypoxic Exercise Wnt/β-Catenin信号通路在低氧运动下减少肥胖大鼠脂肪生成中的作用

American Journal of Biochemistry and Biotechnology Pub Date : 2022-02-01 DOI: 10.3844/ajbbsp.2022.184.194

Pei-Yin Xu, Zhiyuan Sun, Xiaoyi Tian, Yingjie Yang, Qinglu Wang, Xuewen Tian

{"title":"Role of the Wnt/β-Catenin Signaling Pathway in Reducing Lipogenesis of Obese Rats Under Hypoxic Exercise","authors":"Pei-Yin Xu, Zhiyuan Sun, Xiaoyi Tian, Yingjie Yang, Qinglu Wang, Xuewen Tian","doi":"10.3844/ajbbsp.2022.184.194","DOIUrl":"https://doi.org/10.3844/ajbbsp.2022.184.194","url":null,"abstract":": Hypoxic exercise exerts a significant effect on body weight loss and body fat reduction. Methods: In the present study, we constructed an obese rat model and divided rats into the following groups: Normoxic quiet (C), Hypoxic quiet (H), normoxic Exercise (CE), and Hypoxic Exercise (HE). We employed isobaric Tags for Relative and Absolute Quantification (iTRAQ) using proteomic technology to screen the differentially expressed proteins in the four groups. Additionally, we used Reduced-Representation Bisulfite Sequencing (RRBS) to analyze high-throughput DNA methylation profiles in both HE and CE groups. Finally, we screened and analyzed the CpG islands and Differentially Methylated Regions (DMRs) of genes related to the Wnt/β-catenin-signaling pathway. Results: The mRNA levels of gene products related to the Wnt/β-catenin-signaling pathway (i.e., c-myc and β-catenin) were significantly augmented ( P <0.05) in the HE group compared to the CE group; the levels of APC and GSK3β were significantly diminished ( P <0.05). The expression levels of the Wnt/β-catenin-signaling pathway proteins c-myc and β-catenin were also significantly increased in the HE group relative to the CE group ( P <0.05). Among genes related to the Wnt-signaling pathway, the CpG-promoter methylation sites included Fzd2, Fzd9, Wnt16, Wnt2b, Sfrp2, Sfrp1, Sox2, and Sox1; the DMRs included CDC, Fzd10, Gsk-3β, PI3k, Wnt16 and Wnt6. Conclusions: Our present findings provide evidence that hypoxic exercise may inhibit adipogenesis and adipogenesis through canonical Wnt signaling.","PeriodicalId":7412,"journal":{"name":"American Journal of Biochemistry and Biotechnology","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83509062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrasound-Assisted Enzymatic Extraction of Polysaccharides from Waste Corn Bract: Process Optimization, Characterization, Antioxidant and Anti-Diabetic Potentials 超声辅助酶法从废玉米苞片中提取多糖:工艺优化、表征、抗氧化和抗糖尿病作用

American Journal of Biochemistry and Biotechnology Pub Date : 2022-02-01 DOI: 10.3844/ajbbsp.2022.238.251

Yihui Liu, Ya-Yun Li, Na Niu, Yuxing Xie, Wang Zhang, Shuai Dong, Gulei Pu, Chenqi Liu, C. Jiang, M. Cai, Yang Liu, Yanglong Zhang

{"title":"Ultrasound-Assisted Enzymatic Extraction of Polysaccharides from Waste Corn Bract: Process Optimization, Characterization, Antioxidant and Anti-Diabetic Potentials","authors":"Yihui Liu, Ya-Yun Li, Na Niu, Yuxing Xie, Wang Zhang, Shuai Dong, Gulei Pu, Chenqi Liu, C. Jiang, M. Cai, Yang Liu, Yanglong Zhang","doi":"10.3844/ajbbsp.2022.238.251","DOIUrl":"https://doi.org/10.3844/ajbbsp.2022.238.251","url":null,"abstract":": Corn bract is the main agricultural waste of corn production and processing. Little information is focused on its value-added utilization. In the present study, the Ultrasound-Assisted Enzymatic Extraction (UAEE) was used for the first time to extract Corn Bract Polysaccharides (CBPs). The optimum conditions of UAEE for CBPs extraction were: Cellulase amount of 0.7%, enzymolysis for 58 min, and ultrasound treatment for 30 min at 729 W, under which the CBPs yield reached 1.17±0.06%, significantly higher than other methods. CBPs are composed of ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, arabinose, and fructose at molar ratios of 3.82: 4.25: 3.54: 8.11: 32.89: 1.00: 27.96: 12.57: 13.25 and have a molecular weight of 215.7 kDa and a particle size of 255 nm. The surface of CBPs appears to be full of holes and bumps and the infrared spectroscopic analysis confirmed that CBPs possess the typical characteristics of polysaccharides and could be a kind of naturally occurring sulfated polysaccharides. CBPs extracted with the optimized UAEE preferentially scavenge reactive nitrogen species with IC 50 of 1.76±0.14 mg.mL -1 for scavenging hydroxyl radical and have medium inhibitory effects on α -glucosidase with IC 50 of 20.66±0.98% mg.mL -1 . The present contribution provides an efficient UAEE method to obtain the CBPs with antioxidant and anti-diabetic potentials. It will give a useful clue for the comprehensive and value-added exploitation of waste corn bract.","PeriodicalId":7412,"journal":{"name":"American Journal of Biochemistry and Biotechnology","volume":"109 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77156961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0