American Journal of Biochemistry and Biotechnology最新文献_第2页

Assessment of Thyroid Hormone Level Alterations in Hepatitis B-Positive Serum Using a Quantitative Immunofluorescence Assay: A Randomized Controlled Trial 使用定量免疫荧光法评估乙型肝炎阳性血清中甲状腺激素水平的改变:一项随机对照试验

American Journal of Biochemistry and Biotechnology Pub Date : 2023-02-01 DOI: 10.3844/ajbbsp.2023.186.193

Hyeokjun Yun, Hyeokjun Yun, Bo Kyeung Jung, Kyung Cheol Min, Jae Kyung Kim, Jae Kyung Kim

{"title":"Assessment of Thyroid Hormone Level Alterations in Hepatitis B-Positive Serum Using a Quantitative Immunofluorescence Assay: A Randomized Controlled Trial","authors":"Hyeokjun Yun, Hyeokjun Yun, Bo Kyeung Jung, Kyung Cheol Min, Jae Kyung Kim, Jae Kyung Kim","doi":"10.3844/ajbbsp.2023.186.193","DOIUrl":"https://doi.org/10.3844/ajbbsp.2023.186.193","url":null,"abstract":"This retrospective study aimed to assess the differences in serum biomarker levels, specifically thyroid hormone (free T4 and Thyroid-Stimulating Hormone [TSH]) levels, according to the presence or absence of Hepatitis B Virus (HBV) infection. Using a quantitative immunofluorescence assay, we analyzed serum samples from patients with confirmed HBV infection at Dankook University Hospital in Cheonan Province, Republic of Korea, between August 2022 and September 2022. The study population was divided into two groups: Young individuals (<60 years) and older adults (≥60 years). Our findings indicated that differences in thyroid hormone levels between HBV-positive and negative participants were not significant. However, when considering age groups, TSH and free T4 levels in young individuals with HBV-positive serum were 1.78±0.09 µIU/mL (normal range: 0.4-5.0 µIU/mL) and 1.24±0.02 ng/mL (normal range: 0.8-1.9 ng/mL), respectively. In older adults with HBV-positive serum, the corresponding values were 2.22±0.17 µIU/mL and 1.24±0.07 ng/mL, respectively. Our results suggest that the influence of HBV on anti-pituitary hormone levels is age-dependent rather than virus related. Furthermore, the quantitative immunofluorescence method used in this study provides a simpler alternative to enzyme-linked immunosorbent assay or electrochemiluminescence immunoassay for assessing serological thyroid hormone levels in patients with HBV. These findings can aid in the interpretation of diagnostic test results and the timely detection of metabolic changes associated with HBV infection.","PeriodicalId":7412,"journal":{"name":"American Journal of Biochemistry and Biotechnology","volume":"239 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135201888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Responses of Neural Cells (U87) to HCoV-229E and HCoV-OC43 Infections 神经细胞U87对HCoV-229E和HCoV-OC43感染的反应

American Journal of Biochemistry and Biotechnology Pub Date : 2023-02-01 DOI: 10.3844/ajbbsp.2023.169.174

Eun Ju Oh, Jae-Sik Jeon, Qian-Wen Wang, Jae Kyung Kim

引用次数: 0

Sonoporation Enhances Liposome Mediated EcTRAF4 siRNA Delivery in SaB-1 Cells 超声穿孔增强脂质体介导的eb -1细胞中EcTRAF4 siRNA的传递

American Journal of Biochemistry and Biotechnology Pub Date : 2023-02-01 DOI: 10.3844/ajbbsp.2023.152.158

Meng Han Chen, Wang Ping

引用次数: 0

Preparing and Antimicrobial Activity of Hydrogel with Biosynthesized Silver Nanoparticles Using Carex Meyeriana Kunth 银杏生物合成纳米银水凝胶的制备及抑菌活性研究

American Journal of Biochemistry and Biotechnology Pub Date : 2023-02-01 DOI: 10.3844/ajbbsp.2023.138.145

Qihui Shen, Henan Zhu, Hongli Zhou, T. Jiang, Yan Liu

引用次数: 0

Screening of Dominant Cellulose-Degrading Microbe in Humus and Optimisation of its Enzyme Producing Conditions and Application Optimization of Enzyme Production Conditions and Evaluation of Efficient Straw Degradation by Penicillium oxalicum 腐殖质中纤维素降解优势菌的筛选及其产酶条件的优化及应用草青霉产酶条件优化及秸秆高效降解评价

American Journal of Biochemistry and Biotechnology Pub Date : 2023-02-01 DOI: 10.3844/ajbbsp.2023.159.168

Gailing Wang, Chendi Li, Yanan Guo, Lei Guo, Mingcheng Wang, Tongbiao Li, Linglong Xu, Enzhong Li

{"title":"Screening of Dominant Cellulose-Degrading Microbe in Humus and Optimisation of its Enzyme Producing Conditions and Application Optimization of Enzyme Production Conditions and Evaluation of Efficient Straw Degradation by Penicillium oxalicum","authors":"Gailing Wang, Chendi Li, Yanan Guo, Lei Guo, Mingcheng Wang, Tongbiao Li, Linglong Xu, Enzhong Li","doi":"10.3844/ajbbsp.2023.159.168","DOIUrl":"https://doi.org/10.3844/ajbbsp.2023.159.168","url":null,"abstract":": The slow degradation of returned straw adversely affects seedling survival rate. To shorten the degradation time of lignin in maize straw in warm temperate regions, a cellulolytic microbial strain that can secrete polymer with high enzymatic activity was isolated from the humus, which helped to identify an effective strain to expedite the decomposition of returned maize straw. Using the molecular biology techniques of primary screening and re-screening using selective media, the dominant cellulolytic microbes in the humus were identified as Penicillium oxalicum ( P . oxalicum ) (KY781806.1). Inoculum size, initial pH, fermentation time, and temperature were optimized to increase cellulase activity. The activities of sodium Carboxymethyl Cellulose (CMC) and Filter Paper (FPA) were increased by 23.04 and 25.10%, respectively. The ability of P. oxalicum to utilize cellulose was also demonstrated using a filter paper degradation test and maize straw utilization experiment. Finally, the results of a field test showed that the addition of P . oxalicum X 5 caused the returned straw to degrade completely within 30 days.","PeriodicalId":7412,"journal":{"name":"American Journal of Biochemistry and Biotechnology","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78661568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Age-Based Association of Glaucoma with the Prevalence of Diabetes and Hypertension at a National Hospital in Seoul 首尔国立医院青光眼与糖尿病和高血压患病率的年龄相关性研究

American Journal of Biochemistry and Biotechnology Pub Date : 2023-02-01 DOI: 10.3844/ajbbsp.2023.146.151

Sun Jung Lee, T. Jang, Jae Kyung Kim

{"title":"Age-Based Association of Glaucoma with the Prevalence of Diabetes and Hypertension at a National Hospital in Seoul","authors":"Sun Jung Lee, T. Jang, Jae Kyung Kim","doi":"10.3844/ajbbsp.2023.146.151","DOIUrl":"https://doi.org/10.3844/ajbbsp.2023.146.151","url":null,"abstract":": Glaucoma can have profound consequences on vision. Although diabetes and hypertension have been suggested as risk factors for glaucoma, their relevance in epidemiological studies remains controversial. Accordingly, we investigated whether diabetes and hypertension, as well as factors such as age and sex, affected the risk of glaucoma. From May 30, 2022, to June 24, 2022, 1152 patients were selected from a national hospital (Seoul, Korea) to analyze the association among visual field test results, diabetes, and hypertension. Patients who visited the hospital for glaucoma treatment or those referred for glaucoma were randomly selected. The mean deviation value, obtained from visual field test results, is an index of the overall change in the visual field. Based on the mean deviation value, patients with glaucoma were classified into early- (-6<MD<0), middle- (-12<MD<-6), and late-stage (MD<-12) glaucoma groups. Among the 1152 patients, 110 (10%) had diabetes co-occurring with hypertension and 997 (87%) had glaucoma. Among the patients with glaucoma, those with early glaucoma were the most represented and most of them did not have comorbid diabetes or hypertension. Moreover, the incidence of glaucoma was higher in older adults aged ≥60 years. No major sex-based changes in the occurrence of diabetes and hypertension were detected in patients with glaucoma. This retrospective study suggests that diabetes and hypertension may affect glaucoma risk; however, other factors such as age may also play a role in glaucoma progression. Regular medical and ophthalmic examinations are necessary to diagnose and manage glaucoma.","PeriodicalId":7412,"journal":{"name":"American Journal of Biochemistry and Biotechnology","volume":"229 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77578277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Enzymatic Role of Lipid Metabolism in Yarrowia lipolytica Grown on Glycerol 甘油生长的多脂耶氏菌脂质代谢的酶促作用

American Journal of Biochemistry and Biotechnology Pub Date : 2023-02-01 DOI: 10.3844/ajbbsp.2023.128.137

Ruixue Wang, Feifei Xin, Yufei Chang, Wenrui Dang, Huaiyuan Zhang, Yuanda Song

{"title":"The Enzymatic Role of Lipid Metabolism in Yarrowia lipolytica Grown on Glycerol","authors":"Ruixue Wang, Feifei Xin, Yufei Chang, Wenrui Dang, Huaiyuan Zhang, Yuanda Song","doi":"10.3844/ajbbsp.2023.128.137","DOIUrl":"https://doi.org/10.3844/ajbbsp.2023.128.137","url":null,"abstract":": More attention has been paid to the production of commercial fatty acids by microbial fermentation. The basic biochemistry of lipid accumulation in oleaginous microorganisms under nitrogen limitation has been extensively studied. As an oleaginous microorganism, Yarrowia lipolytica can produce fatty acid-derived biofuels and biochemicals. However, the response of the enzymes in Y. lipolytica to glycerol remains unclear. Therefore, we aimed to identify the rate-limiting enzymes associated with the mechanism of lipid accumulation of Y. lipolytica grown on glycerol. The variations in key enzyme activities of fatty acid accumulation in Y. lipolytica were investigated with glycerol as the single carbon source. The nitrogen source was exhausted at 10 h in the medium, leading to substantial changes in key enzyme activities associated with lipid accumulation. Adenosine monophosphate deaminase activity increased immediately by approximately twofold. NAD + -isocitrate dehydrogenase activity decreased by 65% after the nitrogen source was exhausted when compared to the initial maximum activity. ATP citrate lyase activity, which provides the substrate acetyl coenzyme A for lipid biosynthesis, increased by approximately fourfold when compared to the activity before nitrogen depletion. Malic Enzyme (ME) activity decreased considerably after nitrogen was exhausted so that the reducing power Nicotinamide Adenine Dinucleotide Phosphate (NADPH) required for lipid synthesis cannot be produced by ME. However, glucose-6-phosphate dehydrogenase activity increased from 550 nmol·min -1 ·mg -1 -771 nmol·min -1 ·mg -1 , suggesting that the main source of NADPH required for fatty acid accumulation may be provided by the pentose phosphate pathway when Y. lipolytica grew on the medium with glycerol as the only carbon source.","PeriodicalId":7412,"journal":{"name":"American Journal of Biochemistry and Biotechnology","volume":"98 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73371676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Year Identification of Seeds in Peony (Paeonia suffruticosa Andr.) Using Hyperspectral Imaging 牡丹(Paeonia suffruticosa Andr.)种子年份鉴定使用高光谱成像

American Journal of Biochemistry and Biotechnology Pub Date : 2023-02-01 DOI: 10.3844/ajbbsp.2023.175.185

Yakun Zhang, Tingting Li, Libo Wang, Yalin Huang, Xingyang Yang, Hangxing Zhang, Gang Wang, Jinguang Li

{"title":"Year Identification of Seeds in Peony (Paeonia suffruticosa Andr.) Using Hyperspectral Imaging","authors":"Yakun Zhang, Tingting Li, Libo Wang, Yalin Huang, Xingyang Yang, Hangxing Zhang, Gang Wang, Jinguang Li","doi":"10.3844/ajbbsp.2023.175.185","DOIUrl":"https://doi.org/10.3844/ajbbsp.2023.175.185","url":null,"abstract":"Seed storage year is one of the important indicators for evaluating the quality of peony seeds. It is of great significance for the development of the peony industry to carry out rapid and non-destructive year identification of peony seeds to provide a basis for the screening of aged seeds during seed breeding and processing. This study explores the feasibility of using hyperspectral imaging technology combined with machine learning methods to identify the two states of peony seeds (shelled and non-shelled) and then determines the most suitable state for the year identification of peony seeds. The two states of peony seeds (shelled and non-shelled) in 2017, 2018, and 2019 are employed as the research objects. Hyperspectral imaging data of two kinds of peony seeds in the spectral range of 935-1720 nm are collected. The machine learning methods based on the two states of peony seeds (shelled and non-shelled), including partial least squares (PLS-DA), Support Vector Machine (SVM), and Convolutional Neural Network (CNN) classification models, are established and compared. It is found that the optimal year identification models of peony seeds (shelled and non-shelled) based on hyperspectral imaging technology have better recognition effects and the recognition accuracy is more than 99.5%. Moreover, the recognition accuracy of the year identification PLS-DA model established by non-shelled peony seeds is 99.96%, which is better than that of shelled peony seeds at 99.64%. This indicates that year identification of peony seeds based on hyperspectral imaging technology is feasible and efficient and that non-shelled peony seeds are more suitable for the year identification of peony seeds. The results can provide a theoretical and methodological justification for the screening of high-quality peony seeds.","PeriodicalId":7412,"journal":{"name":"American Journal of Biochemistry and Biotechnology","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135201745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial Diversity and Community Structure Characteristics in Rhizosphere Soil of Coconut (Cocos nucifera L.) under Intercropping Pineapple 菠萝间作下椰子根际土壤细菌多样性及群落结构特征

American Journal of Biochemistry and Biotechnology Pub Date : 2023-01-01 DOI: 10.3844/ajbbsp.2023.71.83

Lilan Lu, Hui Zhu, Yingying Liu, Yi Wu, Siting Chen

引用次数: 0

Tetrastigma hemsleyanum Diels et Gilg Roots from Different Origins: Pharmacognostical Identification, Content Determination of Functional Composition and in vitro Antioxidant Activity 不同来源赤柱根的生药学鉴定、功能成分含量测定及体外抗氧化活性研究

American Journal of Biochemistry and Biotechnology Pub Date : 2023-01-01 DOI: 10.3844/ajbbsp.2023.36.46

Guangkuo Li, Jian Ge, Yongli Zheng, Aichun Xu, Qian Li

引用次数: 1