Life metabolism最新文献

Brain-body mitochondrial distribution patterns lack coherence and point to tissue-specific regulatory mechanisms. 脑-体线粒体分布模式缺乏一致性，指向组织特异性调节机制。

Life metabolism Pub Date : 2025-04-12 eCollection Date: 2025-06-01 DOI: 10.1093/lifemeta/loaf012

Jack Devine, Anna S Monzel, David Shire, Ayelet M Rosenberg, Alex Junker, Alan A Cohen, Martin Picard

{"title":"Brain-body mitochondrial distribution patterns lack coherence and point to tissue-specific regulatory mechanisms.","authors":"Jack Devine, Anna S Monzel, David Shire, Ayelet M Rosenberg, Alex Junker, Alan A Cohen, Martin Picard","doi":"10.1093/lifemeta/loaf012","DOIUrl":"10.1093/lifemeta/loaf012","url":null,"abstract":"Energy transformation capacity is generally assumed to be a coherent individual trait driven by genetic and environmental factors. This predicts that some individuals should have consistently high, while others show consistently low mitochondrial oxidative phosphorylation (OxPhos) capacity across organ systems. Here, we test this assumption using multi-tissue molecular and enzymatic assays in mice and humans. Across up to 22 mouse tissues, neither mitochondrial OxPhos capacity nor mitochondrial DNA (mtDNA) density was correlated between tissues (median r = -0.01 to 0.16), indicating that animals with high mitochondrial content or capacity in one tissue may have low content or capacity in other tissues. Similarly, RNA sequencing (RNAseq)-based indices of mitochondrial expression across 45 tissues from 948 women and men (genotype-tissue expression [GTEx]) showed only small to moderate coherence between some tissues, such as between brain regions (r = 0.26), but not between brain-body tissue pairs (r = 0.01). The mtDNA copy number (mtDNAcn) also lacked coherence across human tissues. Mechanistically, tissue-specific differences in mitochondrial gene expression were partially attributable to (i) tissue-specific activation of energy sensing pathways, including the transcriptional coactivator peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α), the integrated stress response (ISR), and other molecular regulators of mitochondrial biology, and (ii) proliferative activity across tissues. Finally, we identify subgroups of individuals with distinct mitochondrial distribution strategies that map onto distinct clinical phenotypes. These data raise the possibility that tissue-specific energy sensing pathways may contribute to idiosyncratic mitochondrial distribution patterns among individuals.","PeriodicalId":74074,"journal":{"name":"Life metabolism","volume":"4 3","pages":"loaf012"},"PeriodicalIF":0.0,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12141818/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144251182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of DNA translocation of chromatin remodeler enzyme Chd1 by exit DNA unwrapping. 染色质重塑酶Chd1的DNA易位调控。

Life metabolism Pub Date : 2025-04-09 eCollection Date: 2025-06-01 DOI: 10.1093/lifemeta/loaf013

Yuanyuan Tian, Qi Jia, Meijing Li, Youyang Sia, Pengjing Hu, Kangjing Chen, Ming Li, Xueming Li, Zigang Xu, Lin Ma, Youpi Ye, Ying Lu, Zhucheng Chen

{"title":"Regulation of DNA translocation of chromatin remodeler enzyme Chd1 by exit DNA unwrapping.","authors":"Yuanyuan Tian, Qi Jia, Meijing Li, Youyang Sia, Pengjing Hu, Kangjing Chen, Ming Li, Xueming Li, Zigang Xu, Lin Ma, Youpi Ye, Ying Lu, Zhucheng Chen","doi":"10.1093/lifemeta/loaf013","DOIUrl":"10.1093/lifemeta/loaf013","url":null,"abstract":"Nucleosomes are the fundamental unit of chromatin. Chromatin remodeler plays a crucial role in the regulation of gene expression in eukaryotes. It is involved in important physiological processes, such as development, immune response, and metabolic regulation. During gene expression regulation, chromatin remodelers slide nucleosomes along genomic DNA and play a major role in chromatin organization. Chd1 senses the extranucleosomal linker DNA and controls nucleosome spacing in cells. However, the mechanism of linker DNA sensing by Chd1 is not completely understood. Here, we report the cryo-electron microscope (cryoEM) structures of Chd1 engaging nucleosomes in different states. Chd1 induces two exit-DNA conformations, either fully wrapped or partially unwrapped states. Notably, in the unwrapped conformation, the exit DNA interacts with a positively charged loop of the motor, named the exit-DNA binding loop, and traps Chd1 in the closed state in the ATPase cycle, suggesting attenuation of its remodeling activity. Explored single-molecule fluorescence resonance energy transfer (smFRET) and biochemical data supported the regulation of Chd1 remodeling activity by the exit-DNA conformations, which is important for the linker DNA sensitivity. Mutants of the Chd1 exit-DNA binding loop compromised nucleosome organization in yeast cells. Together, our findings provide valuable insights into Chd1 regulation by exit DNA unwrapping. These results provide a new perspective for the study of cell development and metabolism.","PeriodicalId":74074,"journal":{"name":"Life metabolism","volume":"4 3","pages":"loaf013"},"PeriodicalIF":0.0,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12125543/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144201013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transporters in vitamin uptake and cellular metabolism: impacts on health and disease. 维生素摄取和细胞代谢中的转运体：对健康和疾病的影响。

Life metabolism Pub Date : 2025-03-10 eCollection Date: 2025-06-01 DOI: 10.1093/lifemeta/loaf008

Yaxuan Yuan, Ligong Chen

{"title":"Transporters in vitamin uptake and cellular metabolism: impacts on health and disease.","authors":"Yaxuan Yuan, Ligong Chen","doi":"10.1093/lifemeta/loaf008","DOIUrl":"10.1093/lifemeta/loaf008","url":null,"abstract":"Vitamins are vital nutrients essential for metabolism, functioning as coenzymes, antioxidants, and regulators of gene expression. Their absorption and metabolism rely on specialized transport proteins that ensure bioavailability and cellular utilization. Water-soluble vitamins, including B-complex and vitamin C, are transported by solute carrier (SLC) family proteins and ATP-binding cassette (ABC) transporters for efficient uptake and cellular distribution. Fat-soluble vitamins (A, D, E, and K) rely on lipid-mediated pathways through proteins like scavenger receptor class B type I (SR-BI), CD36, and Niemann-Pick C1-like 1 (NPC1L1), integrating their absorption with lipid metabolism. Defective vitamin transporters are associated with diverse metabolic disorders, including neurological, hematological, and mitochondrial diseases. Advances in structural and functional studies of vitamin transporters highlight their tissue-specific roles and regulatory mechanisms, shedding light on their impact on health and disease. This review emphasizes the significance of vitamin transporters and their potential as therapeutic targets for deficiencies and related chronic conditions.","PeriodicalId":74074,"journal":{"name":"Life metabolism","volume":"4 3","pages":"loaf008"},"PeriodicalIF":0.0,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12121362/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144183635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dietary sulfur amino acid restriction improves metabolic health by reducing fat mass. 饮食硫氨基酸限制通过减少脂肪量改善代谢健康。

Life metabolism Pub Date : 2025-03-07 eCollection Date: 2025-06-01 DOI: 10.1093/lifemeta/loaf009

Chenhao Xin, Mingcheng Cai, Qianxi Jia, Rong Huang, Rui Li, Junyao Wang, Zi Li, Qiang Zhao, Tianyi Liu, Weidong Zhuang, Jinyu Zhou, Shengxian Li, Yongzhen Tao, Lin Wang, Lifeng Yang

{"title":"Dietary sulfur amino acid restriction improves metabolic health by reducing fat mass.","authors":"Chenhao Xin, Mingcheng Cai, Qianxi Jia, Rong Huang, Rui Li, Junyao Wang, Zi Li, Qiang Zhao, Tianyi Liu, Weidong Zhuang, Jinyu Zhou, Shengxian Li, Yongzhen Tao, Lin Wang, Lifeng Yang","doi":"10.1093/lifemeta/loaf009","DOIUrl":"10.1093/lifemeta/loaf009","url":null,"abstract":"Diet interventions such as calorie restriction or time-restricted feeding offer potential for weight management, but long-term success is often hindered by poor adherence due to the rewarding effects of sugars. In this study, we demonstrate that sulfur amino acid restriction (SAAR) diets promote rapid fat loss without impairing appetite and physiological locomotion, outperforming diets with restricted branched-chain amino acids. Weekly cycling of SAAR diets preserves metabolic benefits, such as reduced fat mass and improved glucose sensitivity. Metabolic analysis and in vivo isotope tracing revealed a shift toward carbohydrate oxidation in white and brown adipose tissue (WAT and BAT), and liver during the SAAR diet refeeding state, leading to decreased de novo lipogenesis. Enhanced lipolysis and fatty acid oxidation were observed in the heart, brain, BAT, lungs, etc. The reintroduction of methionine or cystine negated these metabolic benefits. Further 13C and 2H tracing experiments indicated that cystine, rather than its derivatives like taurine or H2S, directly regulates adiposity. In a high-fat diet model, SAAR diet led to sustained fat mass reduction, regardless of the timing of intervention. Additionally, cystine levels correlated positively with body mass index (BMI) and total triglycerides in diabetic patients. Our findings highlight SAAR diet as a promising strategy for long-term weight control by modulating systemic glucose and lipid metabolism homeostasis.","PeriodicalId":74074,"journal":{"name":"Life metabolism","volume":"4 3","pages":"loaf009"},"PeriodicalIF":0.0,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12141819/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144251183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intermittent fasting promotes HFSC death to inhibit hair growth. 间歇性禁食促进HFSC死亡，抑制头发生长。

Life metabolism Pub Date : 2025-02-19 eCollection Date: 2025-04-01 DOI: 10.1093/lifemeta/loaf006

Abigail Benvie, Valerie Horsley

引用次数: 0

Development of cyclopeptide inhibitors specifically disrupting FXR-coactivator interaction in the intestine as a novel therapeutic strategy for MASH. 环肽抑制剂在肠道中特异性破坏fxr -辅激活物相互作用的发展作为一种新的治疗MASH的策略。

Life metabolism Pub Date : 2025-02-08 eCollection Date: 2025-04-01 DOI: 10.1093/lifemeta/loaf004

Yazhou Li, Tingying Jiao, Xi Cheng, Lu Liu, Mengjiao Zhang, Jian Li, Jue Wang, Shulei Hu, Cuina Li, Tao Yu, Yameng Liu, Yangtai Li, Yu Zhang, Chuying Sun, Jina Sun, Jiang Wang, Cen Xie, Hong Liu

{"title":"Development of cyclopeptide inhibitors specifically disrupting FXR-coactivator interaction in the intestine as a novel therapeutic strategy for MASH.","authors":"Yazhou Li, Tingying Jiao, Xi Cheng, Lu Liu, Mengjiao Zhang, Jian Li, Jue Wang, Shulei Hu, Cuina Li, Tao Yu, Yameng Liu, Yangtai Li, Yu Zhang, Chuying Sun, Jina Sun, Jiang Wang, Cen Xie, Hong Liu","doi":"10.1093/lifemeta/loaf004","DOIUrl":"https://doi.org/10.1093/lifemeta/loaf004","url":null,"abstract":"Intestinal farnesoid X receptor (FXR) antagonists have been proven to be efficacious in ameliorating metabolic diseases, particularly for the treatment of metabolic dysfunction-associated steatohepatitis (MASH). All the reported FXR antagonists target to the ligand-binding pocket (LBP) of the receptor, whereas antagonist acting on the non-LBP site of nuclear receptor (NR) is conceived as a promising strategy to discover novel FXR antagonist. Here, we have postulated the hypothesis of antagonizing FXR by disrupting the interaction between FXR and coactivators, and have successfully developed a series of macrocyclic peptides as FXR antagonists based on this premise. The cyclopeptide DC646 not only exhibits potent inhibitory activity of FXR, but also demonstrates a high degree of selectivity towards other NRs. Moreover, cyclopeptide DC646 has high potential therapeutic benefit for the treatment of MASH in an intestinal FXR-dependent manner, along with a commendable safety profile. Mechanistically, distinct from other known FXR antagonists, cyclopeptide DC646 specifically binds to the coactivator binding site of FXR, which can block the coactivator recruitment, reducing the circulation of intestine-derived ceramides to the liver, and promoting the release of glucagon-like peptide-1 (GLP-1). Overall, we identify a novel cyclopeptide that targets FXR-coactivator interaction, paving the way for a new approach to treating MASH with FXR antagonists.","PeriodicalId":74074,"journal":{"name":"Life metabolism","volume":"4 2","pages":"loaf004"},"PeriodicalIF":0.0,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11992618/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144059897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PCSK9 V474I germline variant drives breast cancer metastasis. PCSK9 V474I种系变异驱动乳腺癌转移

Life metabolism Pub Date : 2025-01-04 eCollection Date: 2025-02-01 DOI: 10.1093/lifemeta/loae041

Hai Wang, Zhiming Shao

引用次数: 0

Glucose-6-phosphate dehydrogenase regulates mitophagy by maintaining PINK1 stability. 葡萄糖-6-磷酸脱氢酶通过维持PINK1的稳定性来调节线粒体自噬。

Life metabolism Pub Date : 2024-12-13 eCollection Date: 2025-02-01 DOI: 10.1093/lifemeta/loae040

Yik-Lam Cho, Hayden Weng Siong Tan, Jicheng Yang, Basil Zheng Mian Kuah, Nicole Si Ying Lim, Naiyang Fu, Boon-Huat Bay, Shuo-Chien Ling, Han-Ming Shen

{"title":"Glucose-6-phosphate dehydrogenase regulates mitophagy by maintaining PINK1 stability.","authors":"Yik-Lam Cho, Hayden Weng Siong Tan, Jicheng Yang, Basil Zheng Mian Kuah, Nicole Si Ying Lim, Naiyang Fu, Boon-Huat Bay, Shuo-Chien Ling, Han-Ming Shen","doi":"10.1093/lifemeta/loae040","DOIUrl":"10.1093/lifemeta/loae040","url":null,"abstract":"Glucose-6-phosphate dehydrogenase (G6PD) is the rate-limiting enzyme in the pentose phosphate pathway (PPP) in glycolysis. Glucose metabolism is closely implicated in the regulation of mitophagy, a selective form of autophagy for the degradation of damaged mitochondria. The PPP and its key enzymes such as G6PD possess important metabolic functions, including biosynthesis and maintenance of intracellular redox balance, while their implication in mitophagy is largely unknown. Here, via a whole-genome CRISPR-Cas9 screening, we identified that G6PD regulates PINK1 (phosphatase and tensin homolog [PTEN]-induced kinase 1)-Parkin-mediated mitophagy. The function of G6PD in mitophagy was verified via multiple approaches. G6PD deletion significantly inhibited mitophagy, which can be rescued by G6PD reconstitution. Intriguingly, while the catalytic activity of G6PD is required, the known PPP functions per se are not involved in mitophagy regulation. Importantly, we found a portion of G6PD localized at mitochondria where it interacts with PINK1. G6PD deletion resulted in an impairment in PINK1 stabilization and subsequent inhibition of ubiquitin phosphorylation, a key starting point of mitophagy. Finally, we found that G6PD deletion resulted in lower cell viability upon mitochondrial depolarization, indicating the physiological function of G6PD-mediated mitophagy in response to mitochondrial stress. In summary, our study reveals a novel role of G6PD as a key positive regulator in mitophagy, which bridges several important cellular processes, namely glucose metabolism, redox homeostasis, and mitochondrial quality control.","PeriodicalId":74074,"journal":{"name":"Life metabolism","volume":"4 1","pages":"loae040"},"PeriodicalIF":0.0,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11749863/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143054456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biphasic glucose-stimulated insulin secretion over decades: a journey from measurements and modeling to mechanistic insights. 几十年来，双相葡萄糖刺激胰岛素分泌：从测量和建模到机制见解的旅程。

Life metabolism Pub Date : 2024-11-19 eCollection Date: 2025-02-01 DOI: 10.1093/lifemeta/loae038

Xiaohong Peng, Kai Wang, Liangyi Chen

{"title":"Biphasic glucose-stimulated insulin secretion over decades: a journey from measurements and modeling to mechanistic insights.","authors":"Xiaohong Peng, Kai Wang, Liangyi Chen","doi":"10.1093/lifemeta/loae038","DOIUrl":"10.1093/lifemeta/loae038","url":null,"abstract":"Glucose-stimulated insulin release from pancreatic β-cells is critical for maintaining blood glucose homeostasis. An abrupt increase in blood glucose concentration evokes a rapid and transient rise in insulin secretion followed by a prolonged, slower phase. A diminished first phase is one of the earliest indicators of β-cell dysfunction in individuals predisposed to develop type 2 diabetes. Consequently, researchers have explored the underlying mechanisms for decades, starting with plasma insulin measurements under physiological conditions and advancing to single-vesicle exocytosis measurements in individual β-cells combined with molecular manipulations. Based on a chain of evidence gathered from genetic manipulation to in vivo mouse phenotyping, a widely accepted theory posits that distinct functional insulin vesicle pools in β-cells regulate biphasic glucose-stimulated insulin secretion (GSIS) via activation of different metabolic signal pathways. Recently, we developed a high-resolution imaging technique to visualize single vesicle exocytosis from β-cells within an intact islet. Our findings reveal that β-cells within the islet exhibit heterogeneity in their secretory capabilities, which also differs from the heterogeneous Ca2+ signals observed in islet β-cells in response to glucose stimulation. Most importantly, we demonstrate that biphasic GSIS emerges from the interactions among α-, β-, and δ-cells within the islet and is driven by a small subset of hypersecretory β-cells. Finally, we propose that a shift from reductionism to holism may be required to fully understand the etiology of complex diseases such as diabetes.","PeriodicalId":74074,"journal":{"name":"Life metabolism","volume":"4 1","pages":"loae038"},"PeriodicalIF":0.0,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11770817/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143054452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chronic cold exposure reprograms feeding-regulated LPL activity in white adipose tissues through hepatic ANGPTL3 and ANGPTL8. 慢性冷暴露通过肝脏ANGPTL3和ANGPTL8重新编程摄食调节的白色脂肪组织中的LPL活性。

Life metabolism Pub Date : 2024-10-16 eCollection Date: 2025-02-01 DOI: 10.1093/lifemeta/loae037

Yiliang Zhang, Shengyang Zhou, Runming Zhao, Yingzhen Huang, Yan Wang

引用次数: 0