Al Mustansiriyah Journal of Pharmaceutical Sciences最新文献

{"title":"The Testicular Protection Effect of Thiamine Pyrophosphate Against Cisplatin-treated Male Rats","authors":"Maitham Abd Ali Mnati, Bahir Abdul Razzaq Mshimesh, Nadia Hamid Mohammed","doi":"10.32947/ajps.v20i4.784","DOIUrl":"https://doi.org/10.32947/ajps.v20i4.784","url":null,"abstract":"Infertility is a worldwide problem affecting both genders, it can be defined as the inability of the adult males to make a fertile woman pregnant after one year of regular intercourse. Cisplatin considers one of the most potent antineoplastic drugs that is extensively \u0000  \u0000used, alone or in combination with other antitumor agents, to manage solid and germ cell cancer. The major drawback in cisplatin treatment is its damaging consequence on various body tissue, including the testis, liver, renal and others. One of its pronounced adverse effects is testicular injury, which may proceed to end with infertility. Thiamine pyrophosphate is the active form of thiamine which has an important role in the oxidative phosphorylation pathway. It acts as a co-factor and energy source for many cellular enzymes, also it utilizes by pentose-phosphate shut that elevates NADPH and improves antioxidants level. This study aimed to evaluate the effect of thiamine pyrophosphate on sperm parameters and gonadotropic hormones (luteal and follicle-stimulating hormone) of male rats exposed to a single dose of cisplatin. \u0000Twenty-eight albino male rats were randomly grouped into four groups. Control group: received normal saline, Cisplatin group: received normal saline and cisplatin, TPP50 group: received thiamine pyrophosphate (50mg/kg) with cisplatin, and TPP100 group: as third group (TPP50) but thiamine pyrophosphate dose was 100 mg/kg. Semen samples used to measure the sperms viability and morphology, while serum samples were gathered to measure the levels of gonadotropic hormones (FSH and LH). \u0000This study revealed that rat’s testicular function was notably deteriorated by cisplatin administration, represented by a reduction in sperm parameters (viability and normal morphology), and serum gonadotropic hormones (FSH and LH). In this work, thiamine pyrophosphate was act as a protective agent that ameliorates rat’s testicular damage induced by cisplatin in a dose-dependent manner. The suggested mechanism may attribute to its antioxidant and anti-apoptotic action","PeriodicalId":7406,"journal":{"name":"Al Mustansiriyah Journal of Pharmaceutical Sciences","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80828325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of gel electrophoresis for proteins and biotechnological products –an overview.","authors":"Ammar Q. Muhammed, Nidhal K. Maraie, Basma T. Al-Sudani","doi":"10.32947/ajps.v20i4.773","DOIUrl":"https://doi.org/10.32947/ajps.v20i4.773","url":null,"abstract":"Nucleic acids or proteins electrophoresed within a matrix or gel that immersed in a buffer provides ions needed to carry a current and for pH maintenance at a relatively constant value. Sodium dodecyl sulphate–polyacrylamide gel electrophoresis \u0000  \u0000(SDS-PAGE) utilizes SDS as an anionic detergent that causes protein denaturation that linearized protein molecules. Each molecule of SDS has the ability to binds to two amino acids. As a result, the ratio of charge to mass becomes constant for all denatured proteins in the mixture. The molecules of protein migrate toward the positive pole and separated in the gel depending only on their molecular weights. The chains of polyacrylamide are cross linked by N, N-methylene bisacrylamide comonomers and ammonium persulfate used as an initiator for polymerization as they act as radical source and N, N, N', N'- tetramethylethylenediamine (TEMED) used to catalyse the polymerization. Electrophoresis of proteins and nucleic acids by using agarose or polyacrylamide gels were illustrated in this review.","PeriodicalId":7406,"journal":{"name":"Al Mustansiriyah Journal of Pharmaceutical Sciences","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86035405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extraction and Detection of Some Flavonoids from Tamarix aphylla (F. Tamaricaceae) Grown in Iraq","authors":"Saja Ismaeel, Huda Jaber Waheed, Widad Mustafa Kamel","doi":"10.32947/ajps.v20i4.785","DOIUrl":"https://doi.org/10.32947/ajps.v20i4.785","url":null,"abstract":"Objective: In the present study, screening for some flavonoids (aglycone and glycoside) in tamarix aphylla by using thin layer chromatography and high-performance liquid chromat-ography. Methods: The leaves of \u0000  \u0000Tamarix.aphylla was extracted by soxhlet with Ethylacetate and then with the ethanol /water 90%. The flavonoids were detected by Analyticl thin layer chromatography using standards and by HPLC. Results:Three  flavonoid aglycones were extracted  with ethylacetate (Kaempferol .Quercetin , Isorhamnetin) and one flavonoid glycoside (Rutin) was detected in the  ethanol /water  90% extract. Conclusion: From   this    study can be conclude that tamarix aphylla is a promising plant for many flavonoid compounds that have many pharmacological actions","PeriodicalId":7406,"journal":{"name":"Al Mustansiriyah Journal of Pharmaceutical Sciences","volume":"25 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91504245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Qualitative, Quantitative and Cytotoxic estimation of Phytochemicals in aerial parts of Iraqi Bauhinia variegata","authors":"Mais Baqer Mohammed, Monther Faisal Mahdi, Ibrahim S. Abbas","doi":"10.32947/ajps.v20i4.786","DOIUrl":"https://doi.org/10.32947/ajps.v20i4.786","url":null,"abstract":"Bauhinia variegata L., is a flowering evergreen small medicinal tree characteristic with an aesthetic appearance and the shape of their flowers which resemble the orchid plant. It is a member of Leguminosae family. Commonly called\" Khuf Al-Jamal\" as in \u0000  \u0000Arabic and \"Camel's feet\" as in English. Quantitative estimation of phenolic compounds using UV spectroscopy. Also, HPLC analysis of lupeol and β-sitosterol content of aerial parts B.variegata. \u0000Moreover, the anticancer activity of stem and leaves hexane extract against AMJ-13 cell was performed, which considered one of the first studies around the world of this plant extracts activity against AMJ-13 cell.","PeriodicalId":7406,"journal":{"name":"Al Mustansiriyah Journal of Pharmaceutical Sciences","volume":"63 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86258643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cuscuta Chinensis potentiate the effect of methotrexate in Rheumatoid Arthritis Induced Rats.","authors":"Ahmed Shawqy Saadoon, Mustafa Ghazi Alabbassi, Nadia Hameed Mohammed","doi":"10.32947/ajps.v20i4.771","DOIUrl":"https://doi.org/10.32947/ajps.v20i4.771","url":null,"abstract":"Background \u0000Rheumatoid arthritis (RA) is a chronic destructive inflammatory disease related to a breakdown in immune tolerance. This disease is characterized by joint inflammation, swelling, and in severe cases deformation may occur. Cuscuta \u0000Chinensis (C. Chinensis) is a parasitic plant, grow around other plants to absorb nutrient and water from them. C. Chinensis has a wide range of chemicals that produce a wide range of pharmacological activates. Because of its anti-oxidant and anti-inflammatory effect, it was considered as a good candidate to assess its role in RA. \u0000Methods: \u0000Rheumatoid arthritis was induced by injection of Complete Freund’s Adjuvant inside the foot-pad of male albino rats. The animals were grouped in four groups as follows group 1 considered as a normal control group, group 2 consider as positive control arthritis, group 3 treated with methotrexate (MTX), group 4 treated with MTX and C. Chinensis extract. On day 14 of immunization, treatments began and last for 21 days, at the end of the experiment all animals were sacrificed and serum was collected. The serum markers that had been evaluated were MMP3, VEGF, and SOD. Throughout the experiment time the body weight was evaluated. \u0000Results \u0000The combination significantly (P-value ≤ 0.05) improves objective parameter of RA which was the body weight. Also significantly decrease (P-value ≤ 0.05) the serum level of MMP3, VEGF, and considerably increase serum SOD. \u0000Conclusion: \u0000The combination has a significant beneficial role in suppression of destructive enzyme (MMP3), angiogenic stimulators (VEGF), and increase serum SOD enzyme.","PeriodicalId":7406,"journal":{"name":"Al Mustansiriyah Journal of Pharmaceutical Sciences","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79644901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GC-MS analysis of Iraqi Silybum marianum Flowers, Leaves and Seeds Extracts","authors":"Rasha Eldalawy, Wasan Abdul kareem, Widad M K Al-Ani","doi":"10.32947/ajps.v20i4.780","DOIUrl":"https://doi.org/10.32947/ajps.v20i4.780","url":null,"abstract":"Iraqi land is rich with medicinal plants and Iraqi people trust using herbals as treatments since ancient times, one of these important wild medicinal plants is Silybum marianum, which is known as Milk thistle. It has a long history in Greek and Arabian medicine as a liver tonic \u0000which has been confirmed by scientific in vivo/ in vitro study and made the plant in the top of medicine that can regenerate liver tissues and cure various liver diseases. The aim of this study is to investigate the active constituents in the seeds, leaves and the flowers of Iraqi Silybum marianum, identification  have been done by GC-MS instrument after the extraction process by hexane then ethanol as a solvent in the Soxhlet apparatus, An important compounds has been detected such as phytosterol, catechin, terpenes, fatty acids, fatty alcohol, and  monosaccharide,  in different concentration in the parts of the plants.","PeriodicalId":7406,"journal":{"name":"Al Mustansiriyah Journal of Pharmaceutical Sciences","volume":"71 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86079240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two-intervention social distancing strategy to control COVID-19 in Mosul city; A Comparative study","authors":"Muhammad A. Ahmed, Zena N. Al Jalelli, Muataz A. Alani, Firas I. Mostafa, Aws A. Al-Hamdani, Rowa fawaz, Saraa M. Ghanim, Zahraa A. Al-Kataan","doi":"10.32947/ajps.v20i3.760","DOIUrl":"https://doi.org/10.32947/ajps.v20i3.760","url":null,"abstract":"Background: Novel coronavirus what known now as COVID-19 pandemic represents worldwide health problem. It presented as an upper respiratory infection, acute respiratory distress syndrome, micro thrombosis, sepsis, septic shock, and multi-organ failure and death. Early recognition of suspected cases allows health staff and local gover- \u0000  \u0000Nment the sufficient time to initiate the appropriate international and national protocols to control the transmission of infection. \u0000Aim:  Evaluate the effectiveness of two- intervention social distancing strategy that was applied to control COVID-19 in Mosul city. \u0000Method: Using mathematical simulation model for COVID-19 spread implemented in Santa Clara City in US to estimate the expected burden of the pandemic in Mosul City. This model give a numerical data about the number of the infected cases and time interval of applying the appropriate intervention control strategies by comparing single-strategy (social distancing) to two-intervention strategy (social distancing & light switch approach). \u0000Results: The two interventions strategy shows that on average, the baseline epidemic scenario affected 12.3% of the population in comparison to 11.6% with single-strategy at 70% contact rate. While at the rate of 20% of social contact, only 0.11 % of the population will be infected in the two-intervention strategy in comparison to 0.165% when the single strategy applied. This means a significant reduction in the spread of the infection and the peak time incidence in all hypotheses will be significantly delayed. \u0000Conclusion: The two- intervention social distancing strategy is an efficient tool to control COVID-19 in Mosul city and this represents a big success for Crisis Cell in controlling the pandemic in Mosul city.","PeriodicalId":7406,"journal":{"name":"Al Mustansiriyah Journal of Pharmaceutical Sciences","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74693077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highlight on lipids and its use for covalent and non-covalent conjugations","authors":"Abdullah Q. Khudhur, Nidhal K. Maraie, Ayad M.R. Raauf","doi":"10.32947/ajps.v20i3.754","DOIUrl":"https://doi.org/10.32947/ajps.v20i3.754","url":null,"abstract":"Lipids are organic fatty or waxy compounds which are used to make nanocarriers that are promising for drug delivery. When lipids associated covalently (lipid-drug conjugate LDC) or non- covalently (drug-lipid complex) to drugs to form Lipid Drug Conjugates (LDC). Most common types of lipids \u0000  \u0000used for drug conjugation are fatty acids, glycerides, steroids, and phospholipids. Conjugation with lipids may change the properties of the drug and significantly increase the drug lipophilicity. Lipid-drug conjugation could improve the delivery of drugs by the lymphatic system, enhance bioavailability of oral administered drugs, improve drug targeting in tumor diseases, enhance the loading of drugs into some delivery carriers, increase drug stability, and many others. Lipid-drug conjugates can be prepared through different strategies for conjugation and by chemical linkers depending on the chemical structure of both drugs and lipids, careful selection of lipids and drug are necessary in designing the lipid-drug conjugate to achieve maximum benefits.","PeriodicalId":7406,"journal":{"name":"Al Mustansiriyah Journal of Pharmaceutical Sciences","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77243433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacological and Pharmacognosticl Activity of Silybum marianum","authors":"Huda Khalil Al-Bazaz, Muthanna I. Al-Ezzi, Ghaith Ali Jasim","doi":"10.32947/ajps.v20i3.762","DOIUrl":"https://doi.org/10.32947/ajps.v20i3.762","url":null,"abstract":"Herbs have been utilized for all time as the significant sours of medication. Medical plants are significant by optional metabolites, for example; Silybum marianum, is a remedial herb with a thousand years history of utilization. It is a blend of flavonoids, called silybin, which isn't just the major silymarin component but at the \u0000same time is the most dynamic element of this extract, which has been affirmed in different studies. \u0000This compound has a place with the flavonoid group known as flavonolignan. Silybin's structure comprises in two fundamental units. The first depends on a taxifolins, the second a phenyllpropanoid unit, which for this situation is conyferil liquor. These two units are connected together into one structure by an oxeran ring contains mixes (taxifolin, silychristin, silydianin, silybinin A and silybinin B. \u0000The present study is fundamentally centered on the medicinal important of Silybum marianum, its utility as a medicinal plant for the treatment of different issue of mind, cardiovascular, hepatic, kidney, and oxidative stress also, malignant growth is outstanding. As far as its medicinal properties, Silybum has no symptoms. In any case, it might cause mild nausea or gastrointestinal difficulties in uncommon cases. The leaves, seeds or some of the time the entire plant is utilized inmedicinal preparation.","PeriodicalId":7406,"journal":{"name":"Al Mustansiriyah Journal of Pharmaceutical Sciences","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91030234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipid Profile Changes in Pregnant Women with Pre-Eclampsia and Their Correlation with Severety of Pre-Eclampsia","authors":"Niran Kamel*, Wasan Munim, Wasan Munim, Alaa Raheem Kareem, Mustafa Rasool Hussein Aal-Saleh, Mohammed Luay Subhi, Sarah Abdulkareem Ali Al-Dujaili","doi":"10.32947/ajps.v20i3.766","DOIUrl":"https://doi.org/10.32947/ajps.v20i3.766","url":null,"abstract":"Objectives: To find out whether there is a lipid profile changes in pregnant women with preeclampsia and if we can use these lipid profile changes as markers of the severity of preeclampsia (for follow up to avoid leaving the patient reaching ecplamptic stage). \u0000  \u0000Design: A prospective case-control study. \u0000Setting: AL- Kadhimiya Teaching hospital/department of Obstetrics and Gynecology. \u0000Materials and Methods: The study included 120 pregnant women in the third trimester. They were divided into three groups. The study group consists of 60 preeclamptic and eclamptic women, 25 women had mild pre-eclampsia and 35 women with severe preeclampsia. The control group consists of 60 normotensive pregnant women. Blood sample was drown after 8-10 hours fasting. Triglyceride, total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol and very low-density lipoprotein cholesterol levels were measured. \u0000Results: Triglyceride and VLDL-C level of severe Preeclampsia group and mild Preeclampsia group was higher than the control group, but the difference between the severe PE group and the control group was statistically significant (p<0.05). Serum cholesterol in the severe PE group was significantly higher than in mild PE group and in the control group (p<0.05). LDL-C and HDL-C levels were determined similarly in all groups (p>0.05). A highly significant positive correlation between the level of proteinuria and serum cholesterol levels, LDL-C, TG and VLDL-C levels. While there was significantly negative correlation between proteinuria and HDL-C levels. Also, a significant positive correlation between the systolic-tension and serum cholesterol levels, TG, VLDL-C levels and among the diastolic-tension and with LDL-C, TG, VLDL-C levels. \u0000Conclusion: Changes in levels of lipid profile are related with preeclampsia, especially with severe preeclampsia.","PeriodicalId":7406,"journal":{"name":"Al Mustansiriyah Journal of Pharmaceutical Sciences","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89098110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}