Gabriel Cruz-González MD , James F. Meschia MD , Benjamin J. Madden BSc , Mercedes Prudencio PhD , Camilo A. Polania-Sandoval MD , Janelle Hartwell BS , Eniola Oyefeso BS , Ranya Benchaaboune , Tara Brigham MLIS , Sukhwinder J.S. Sandhu MD , Cristine Charlesworth PhD, MS , Ganesh P. Pujari MD , Leonard Petrucelli PhD , Akhilesh Pandey MD, PhD , Young Erben MD
{"title":"Recent advances in proteomic analysis to study carotid artery plaques","authors":"Gabriel Cruz-González MD , James F. Meschia MD , Benjamin J. Madden BSc , Mercedes Prudencio PhD , Camilo A. Polania-Sandoval MD , Janelle Hartwell BS , Eniola Oyefeso BS , Ranya Benchaaboune , Tara Brigham MLIS , Sukhwinder J.S. Sandhu MD , Cristine Charlesworth PhD, MS , Ganesh P. Pujari MD , Leonard Petrucelli PhD , Akhilesh Pandey MD, PhD , Young Erben MD","doi":"10.1016/j.jvssci.2024.100215","DOIUrl":"10.1016/j.jvssci.2024.100215","url":null,"abstract":"<div><h3>Objective</h3><p>We sought to identify differentially expressed proteins in serum, plasma, and plaque samples of patients with carotid atherosclerotic lesions.</p></div><div><h3>Methods</h3><p>We performed a systematic review of the proteomic profile of serum, plasma, and plaque samples of patients with carotid artery disease. We included full-length peer-reviewed studies of adult humans and reported them using PRISMA guidelines. The quality of the design and content of the articles included in the review was assessed using the Newcastle-Ottawa scale.</p></div><div><h3>Results</h3><p>We included six peer-reviewed articles reporting protein expression in serum, plasma, or plaque samples from patients with carotid atherosclerosis. Three were single-center cross-sectional studies, two were single-center case-control studies, and one was a single-center cohort study. Thirty-six proteins were found to be expressed differentially when comparing samples from healthy subjects and individuals with diseased carotid vessels and between patients with symptomatic and asymptomatic carotid artery atherosclerotic lesions. Some of these were shown to be related to inflammatory or anti-inflammatory pathways in atherogenesis. CD5L and S100A12 were both found to be upregulated in patients with unstable plaque, the former owing to its anti-inflammatory properties and the latter for its pro-oxidant effects in atherosclerosis. ACTB is involved in cellular structure and integrity and was found to be downregulated in patients with ruptured carotid plaques.</p></div><div><h3>Conclusions</h3><p>Atherosclerotic carotid disease places the patient at increased risk of ischemic neurological events. Proteomics may help to understand their pathophysiological processes and can identify differential protein expression in blood samples from healthy subjects and patients with carotid artery plaques. This patient-centered approach will allow for the timely identification of individuals at higher risk of experiencing stroke.</p></div>","PeriodicalId":74035,"journal":{"name":"JVS-vascular science","volume":"5 ","pages":"Article 100215"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666350324000269/pdfft?md5=0724ce0ddb26f766af2d098433cbd988&pid=1-s2.0-S2666350324000269-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142270515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Thijs J. Sluiter MSc , Lucinda Cruddas MBBS , Federica Ninno MRes , Thomas Schmitz-Rixen MD, PhD , Janice Tsui MD, FRCS , Margreet R. de Vries PhD
{"title":"Basic Vascular Science 2024 Meeting","authors":"Thijs J. Sluiter MSc , Lucinda Cruddas MBBS , Federica Ninno MRes , Thomas Schmitz-Rixen MD, PhD , Janice Tsui MD, FRCS , Margreet R. de Vries PhD","doi":"10.1016/j.jvssci.2024.100210","DOIUrl":"10.1016/j.jvssci.2024.100210","url":null,"abstract":"<div><p>The Basic Vascular Science (BVS) meeting was set up to provide a forum for researchers and clinicians in the field to exchange knowledge and ideas and to foster cross-disciplinary collaborations. The BVS 2024 meeting was held in Berlin. Attended by vascular surgeons and physicians, interventional radiologists, basic science researchers, and engineers, the meeting continues to successfully attract both early career researchers and established clinician-scientists. Here, we report on the scientific sessions encompassing keynote lectures and oral presentations.</p></div>","PeriodicalId":74035,"journal":{"name":"JVS-vascular science","volume":"5 ","pages":"Article 100210"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S266635032400021X/pdfft?md5=a901ea037bdb368d4a77d022a15f8357&pid=1-s2.0-S266635032400021X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141276653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Michael G. Levin, Shaunak Adkar, Jonas Ghouse, John Depaolo, Ole BV. Pedersen, Sisse Ostrowski, Julie Lynch, Henning Bundgaard, Scott M. Damrauer, Derek Klarin
{"title":"Genetic Variation Associated With Progression and Severity of Peripheral Artery Disease","authors":"Michael G. Levin, Shaunak Adkar, Jonas Ghouse, John Depaolo, Ole BV. Pedersen, Sisse Ostrowski, Julie Lynch, Henning Bundgaard, Scott M. Damrauer, Derek Klarin","doi":"10.1016/j.jvssci.2024.100237","DOIUrl":"10.1016/j.jvssci.2024.100237","url":null,"abstract":"","PeriodicalId":74035,"journal":{"name":"JVS-vascular science","volume":"5 ","pages":"Article 100237"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143131244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shaunak Adkar, Sabina Sorondo, Mozhgan Lotfi, Sharika Bamezai, Ryan Choi, Ole Dyg Pedersen, Sisse Ostrowski, Jonas Ghouse, Henning Bundgaard, Michael Levin, Scott M. Damrauer, Nicholas J. Leeper, Derek Klarin
{"title":"Soluble Guanylate Cyclase Activators as Potential Novel Pad Therapeutics","authors":"Shaunak Adkar, Sabina Sorondo, Mozhgan Lotfi, Sharika Bamezai, Ryan Choi, Ole Dyg Pedersen, Sisse Ostrowski, Jonas Ghouse, Henning Bundgaard, Michael Levin, Scott M. Damrauer, Nicholas J. Leeper, Derek Klarin","doi":"10.1016/j.jvssci.2024.100218","DOIUrl":"10.1016/j.jvssci.2024.100218","url":null,"abstract":"","PeriodicalId":74035,"journal":{"name":"JVS-vascular science","volume":"5 ","pages":"Article 100218"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143139820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jillian Poles, Lakshmi Arivazhagan, Vikas Kumar, Ann Marie Marie Schmidt
{"title":"Cofilin 1 Mediates Fatty Acid-Induced Foam Cell Formation And Inflammation In Mouse Bone Marrow-derived Macrophages","authors":"Jillian Poles, Lakshmi Arivazhagan, Vikas Kumar, Ann Marie Marie Schmidt","doi":"10.1016/j.jvssci.2024.100240","DOIUrl":"10.1016/j.jvssci.2024.100240","url":null,"abstract":"","PeriodicalId":74035,"journal":{"name":"JVS-vascular science","volume":"5 ","pages":"Article 100240"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143151970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dakota W. Gonring BA , Zachary R. Zottola BS , Adnan A. Hirad MD, PhD , Ronald Lakony BS , Michael S. Richards PhD , Grayson Pitcher MD , Michael C. Stoner MD , Doran S. Mix MD
{"title":"Ultrasound elastography to quantify average percent pressure-normalized strain reduction associated with different aortic endografts in 3D-printed hydrogel phantoms","authors":"Dakota W. Gonring BA , Zachary R. Zottola BS , Adnan A. Hirad MD, PhD , Ronald Lakony BS , Michael S. Richards PhD , Grayson Pitcher MD , Michael C. Stoner MD , Doran S. Mix MD","doi":"10.1016/j.jvssci.2024.100198","DOIUrl":"10.1016/j.jvssci.2024.100198","url":null,"abstract":"<div><h3>Objective</h3><p>Strain has become a viable index for evaluating abdominal aortic aneurysm stability after endovascular aneurysm repair (EVAR). In addition, literature has shown that healthy aortic tissue requires a degree of strain to maintain homeostasis. This has led to the hypothesis that too much strain reduction conferred by a high degree of graft oversizing is detrimental to the aneurysm neck in the seal zone of abdominal aortic aneurysms after EVAR. We investigated this in a laboratory experiment by examining the effects that graft oversizing has on the pressure-normalized strain (<span><math><mrow><mover><msub><mi>ε</mi><mrow><mi>ρ</mi><mo>+</mo></mrow></msub><mo>¯</mo></mover></mrow></math></span>/pulse pressure [PP]) reduction using four different infrarenal EVAR endografts and our ultrasound elastography technique. Approximate graft oversizing percentages were 20% (30 mm phantom-graft combinations), 30% (28 mm phantom-graft combinations), and 50% (24 mm phantom-graft combinations).</p></div><div><h3>Methods</h3><p>Axisymmetric, 10% by mass polyvinyl alcohol phantoms were connected to a flow simulator. Ultrasound elastography was performed before and after implantation with the four different endografts: (1) 36 mm polyester/stainless steel, (2) 36 mm polyester/electropolished nitinol, (3) 35 mm polytetrafluoroethylene (PTFE)/nitinol, and (4) 36 mm nitinol/polyester/platinum-iridium. Five ultrasound cine loops were taken of each phantom-graft combination. They were analyzed over two different cardiac cycles (end-diastole to end-diastole), yielding a total of 10 maximum mean principal strain (<span><math><mrow><mover><msub><mi>ε</mi><mrow><mi>ρ</mi><mo>+</mo></mrow></msub><mo>¯</mo></mover></mrow></math></span>) values. <span><math><mrow><mover><msub><mi>ε</mi><mrow><mi>ρ</mi><mo>+</mo></mrow></msub><mo>¯</mo></mover></mrow></math></span> was divided by pulse pressure to yield pressure-normalized strain (<span><math><mrow><mover><msub><mi>ε</mi><mrow><mi>ρ</mi><mo>+</mo></mrow></msub><mo>¯</mo></mover></mrow></math></span>/PP). An analysis of variance was performed for graft comparisons. We calculated the average percent <span><math><mrow><mover><msub><mi>ε</mi><mrow><mi>ρ</mi><mo>+</mo></mrow></msub><mo>¯</mo></mover></mrow></math></span>/PP reduction by manufacturer and percent oversizing. These values were used for linear regression analysis.</p></div><div><h3>Results</h3><p>Results from one-way analysis of variance showed a significant difference in <span><math><mrow><mover><msub><mi>ε</mi><mrow><mi>ρ</mi><mo>+</mo></mrow></msub><mo>¯</mo></mover></mrow></math></span>/PP between the empty phantom condition and all oversizing conditions for all graft manufacturers (<em>F</em>(3, 56) = 106.7 [graft A], 132.7 [graft B], 106.5 [graft C], 105.7 [graft D], <em>P</em> < .0001 for grafts A-D). There was a significant difference when comparing the 50% condition with the 30% and 20% conditions across all manufacturers by post hoc a","PeriodicalId":74035,"journal":{"name":"JVS-vascular science","volume":"5 ","pages":"Article 100198"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666350324000099/pdfft?md5=174462f0b3289da4a0f6e8331d8e0f44&pid=1-s2.0-S2666350324000099-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140270596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xiao Luo PhD , Fattah Muhammad Tahabi BS , Dave M. Rollins RVT , Alan P. Sawchuk MD
{"title":"Predicting future occlusion or stenosis of lower extremity bypass grafts using artificial intelligence to simultaneously analyze all flow velocities collected in current and previous ultrasound examinations","authors":"Xiao Luo PhD , Fattah Muhammad Tahabi BS , Dave M. Rollins RVT , Alan P. Sawchuk MD","doi":"10.1016/j.jvssci.2024.100192","DOIUrl":"10.1016/j.jvssci.2024.100192","url":null,"abstract":"<div><h3>Objective</h3><p>Routine surveillance with duplex ultrasound (DUS) examination is recommended after femoral-popliteal and femoral-tibial-pedal vein bypass grafts with various intervals postoperatively. The presently used methodology to analyze bypass graft DUS examination does not use all the available data and has been shown to have a significant rate for missing impending bypass graft failure. The objective of this research is to investigate recurrent neural networks (RNNs) to predict future bypass graft occlusion or stenosis.</p></div><div><h3>Methods</h3><p>This study includes DUS examinations of 663 patients who had bypass graft operations done between January 2009 and June 2022. Only examinations without missing values were included. We developed two RNNs (a bidirectional long short-term memory unit and a bidirectional gated recurrent unit) to predict bypass graft occlusion and stenosis based on peak systolic velocities collected in the 2 to 5 previous DUS examinations. We excluded the examinations with missing values and split our data into training and test sets. Then, we applied 10-fold cross-validation on training to optimize the hyperparameters and compared models using the test data.</p></div><div><h3>Results</h3><p>The bidirectional long short-term memory unit model can gain an overall sensitivity of 0.939, specificity of 0.963, and area under the curve of 0.950 on the prediction of bypass graft occlusion, and an overall sensitivity of 0.915, specificity of 0.909, and area under the curve of 0.912 predicting the development of a future critical stenosis. The results on different bypass types show that the system performs differently on different types. The results on subcohorts based on gender, smoking status, and comorbidities show that the performance on current smokers is lower than the never smoker.</p></div><div><h3>Conclusions</h3><p>We found that RNNs can gain good sensitivity, specificity, and accuracy for the detection of impending bypass graft occlusion or the future development of a critical bypass graft stenosis using all the available peak systolic velocity data in the present and previous bypass graft DUS examinations. Integrating clinical data, including demographics, social determinants, medication, and other risk factors, together with the DUS examination may result in further improvements.</p></div><div><h3>Clinical Relevance</h3><p>Detecting bypass graft failure before it occurs is important clinically to prevent amputations, salvage limbs, and save lives. Current methods evaluating screening duplex ultrasound examinations have a significant failure rate for detecting a bypass graft at risk for failure. Artificial intelligence using recurrent neural networks has the potential to improve the detection of at-risk bypass graft before they fail. Additionally, artificial intelligence is in the news and is being applied to many fields. Vascular surgeons need to know its potential to improve vascular outcomes.</p","PeriodicalId":74035,"journal":{"name":"JVS-vascular science","volume":"5 ","pages":"Article 100192"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666350324000038/pdfft?md5=e64b464b16fd4434244d34e5952f1fb4&pid=1-s2.0-S2666350324000038-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139814472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
